FastQCFastQC Report
Thu 26 May 2016
SRR936505_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936505_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2325229
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT344621.482090581185767No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT161910.6963185131442968No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT126230.5428712612822221No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC117830.5067457871891328No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT93960.4040892316412706No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC90980.39127328964157937No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC87810.3776402238231159No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT81290.3495999748841942No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG71820.30887280349591373No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA64560.27765007231545796No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC64290.2764888963624658No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57350.24664237371888964No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA52510.22582721959858576No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51150.2199783333168475No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC42400.18234763113654612No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA36340.1562856819693888No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC34710.1492756197346584No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT34390.1478994111977788No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT31840.13693274941951955No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG30210.1299226871847891No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28390.12209550113128642No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG28060.12067628607762934No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC27320.11749380383609528No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC26350.11332217170867902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG101050.024.1718391
GTACCGT1201.7117341E-423.7496226
TACATGG104200.022.7496972
ACATGGG102000.022.5398273
GAGTACT71600.021.46205712-13
GTAAGGT9650.020.672364
GTATAGG7200.020.4603041
CATGGGG51650.020.2311424
CGCGGAA2350.020.21179482-83
TACCGTG1707.959288E-519.5585167
GTACTTT78350.019.55222114-15
GGTAAGG10700.019.5332413
TAAGGTG11700.019.0804845
CTCGCGC2500.018.99990360-61
AGTACTT77450.018.70632712-13
AGAGTAC134150.018.2187610-11
CATGGGA50450.017.6996524
TACCTGG15350.017.6404322
CGATCGT2850.017.50047524-25
ACTTTTT90450.017.197916-17
Produced by FastQC (version 0.11.5)