##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936505_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2325229 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.88911113701059 31.0 31.0 34.0 30.0 34.0 2 32.02797745942443 31.0 31.0 34.0 30.0 34.0 3 32.150004580194036 33.0 31.0 34.0 30.0 34.0 4 35.866294029534295 37.0 35.0 37.0 35.0 37.0 5 35.64879115132316 37.0 35.0 37.0 33.0 37.0 6 35.7609340843418 37.0 35.0 37.0 33.0 37.0 7 35.708504409673196 37.0 35.0 37.0 33.0 37.0 8 35.68712802050895 37.0 35.0 37.0 33.0 37.0 9 37.32481961991701 39.0 37.0 39.0 34.0 39.0 10-11 37.18801997566691 39.0 37.0 39.0 33.0 39.0 12-13 37.110253011638854 39.0 37.0 39.0 33.0 39.0 14-15 38.18779935223584 40.0 37.0 41.0 33.0 41.0 16-17 38.14116480570301 40.0 37.0 41.0 33.0 41.0 18-19 38.300350417098706 40.0 38.0 41.0 33.5 41.0 20-21 38.328017799537164 40.0 38.0 41.0 34.0 41.0 22-23 38.235807526914556 40.0 38.0 41.0 33.5 41.0 24-25 38.1042882227944 40.0 37.0 41.0 33.0 41.0 26-27 37.8848803709226 40.0 37.0 41.0 33.0 41.0 28-29 37.838595252338585 40.0 37.0 41.0 33.0 41.0 30-31 37.869118912588824 40.0 37.0 41.0 33.0 41.0 32-33 37.747384021100714 40.0 37.0 41.0 33.0 41.0 34-35 37.56528174214239 40.0 37.0 41.0 32.5 41.0 36-37 37.30914761513812 39.5 36.0 41.0 31.5 41.0 38-39 37.16287040975319 39.0 36.0 41.0 31.0 41.0 40-41 37.02822539199365 39.0 36.0 41.0 31.0 41.0 42-43 36.789393646819306 39.0 35.5 41.0 31.0 41.0 44-45 36.5736144698006 39.0 35.0 40.0 30.0 41.0 46-47 36.28501966903045 39.0 35.0 40.0 30.0 41.0 48-49 35.955787365459486 39.0 35.0 40.0 29.0 41.0 50-51 35.64850257759558 38.0 35.0 40.0 28.0 41.0 52-53 35.41502084310835 38.0 34.5 40.0 28.0 41.0 54-55 35.17224002453092 38.0 34.0 40.0 27.0 41.0 56-57 34.81773838189701 38.0 34.0 40.0 26.5 41.0 58-59 34.47671842214251 37.5 33.5 40.0 26.0 41.0 60-61 34.05163620443406 37.0 33.0 39.5 26.0 41.0 62-63 33.69123815331737 36.0 33.0 39.0 25.0 40.5 64-65 33.29379579387664 36.0 32.0 39.0 23.5 40.0 66-67 32.7875559783574 35.5 31.5 38.5 22.5 40.0 68-69 32.47875800620068 35.0 31.0 38.0 22.0 40.0 70-71 32.003113241749524 35.0 31.0 37.5 21.5 39.5 72-73 31.534297482097465 34.5 30.5 37.0 20.5 39.0 74-75 31.029392803891575 34.0 30.0 36.0 18.5 39.0 76-77 29.593046104276183 32.5 28.5 35.0 18.0 37.0 78-79 30.39924261223303 34.0 30.0 35.0 18.0 37.0 80-81 30.162125751915188 34.0 30.0 35.0 17.0 37.0 82-83 29.818095766051428 34.0 29.5 35.0 13.0 36.5 84-85 29.400011138687844 34.0 29.0 35.0 8.5 36.0 86-87 29.101221213050415 34.0 29.0 35.0 7.0 36.0 88-89 28.700458750514464 33.0 29.0 35.0 2.0 35.0 90-91 28.191434263033877 33.0 27.5 35.0 2.0 35.0 92-93 27.712962250169767 33.0 27.0 35.0 2.0 35.0 94-95 27.1041521071688 32.5 25.5 34.0 2.0 35.0 96-97 26.32697446144014 32.0 25.0 34.0 2.0 35.0 98-99 25.399613758472825 32.0 22.0 34.0 2.0 35.0 100-101 22.132794447342604 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4279087063840272 1101 2 0.4976784136491972 1101 3 0.41732839810963895 1101 4 0.2883979869447373 1101 5 0.2678701404710253 1101 6 0.397657556508598 1101 7 0.36396402501176084 1101 8 0.31898824805099935 1101 9 0.43664362805455426 1101 10-11 0.41303963736866933 1101 12-13 0.4681463186187429 1101 14-15 0.6106947926105093 1101 16-17 0.6098348621439484 1101 18-19 0.6062691324684835 1101 20-21 0.46029545634236513 1101 22-23 0.5309978177124748 1101 24-25 0.42930171173414067 1101 26-27 0.3983996016950684 1101 28-29 0.4199823067772783 1101 30-31 0.5819410524527839 1101 32-33 0.6339656500591246 1101 34-35 0.5066244107491613 1101 36-37 0.5631958594196789 1101 38-39 0.4397959695988618 1101 40-41 0.4881640233865241 1101 42-43 0.6586440115383638 1101 44-45 0.682487431582409 1101 46-47 0.6360440132743364 1101 48-49 0.5455036239980302 1101 50-51 0.519956945206097 1101 52-53 0.6046678028775432 1101 54-55 0.5007834987296604 1101 56-57 0.5256186139187307 1101 58-59 0.38027250060808626 1101 60-61 0.46374964918941686 1101 62-63 0.4114436624474891 1101 64-65 0.4151187863877439 1101 66-67 0.28336342103514056 1101 68-69 0.24377272898365732 1101 70-71 0.4174102182259922 1101 72-73 0.34876704324148733 1101 74-75 0.1258634807736101 1101 76-77 0.1024441471736246 1101 78-79 -0.19496280737928018 1101 80-81 -0.04115161980825022 1101 82-83 0.29208960552784546 1101 84-85 0.31554223562032746 1101 86-87 0.3565540887383811 1101 88-89 0.2653473702196081 1101 90-91 0.24011126015557593 1101 92-93 0.13740567912961765 1101 94-95 0.4330049370839788 1101 96-97 0.5001343409212851 1101 98-99 0.5349498927194531 1101 100-101 1.0412979918454504 1102 1 0.299665524401302 1102 2 0.3451631662811039 1102 3 0.34034853324153147 1102 4 0.20490019299052875 1102 5 0.21233640891954053 1102 6 0.21059650439384825 1102 7 0.27027464882636565 1102 8 0.33983566860008096 1102 9 0.38531083840236846 1102 10-11 0.4016947329548799 1102 12-13 0.3767763689648618 1102 14-15 0.552072557924852 1102 16-17 0.5084430110774534 1102 18-19 0.39450620242908485 1102 20-21 0.25661144086091525 1102 22-23 0.38123024823457285 1102 24-25 0.39506882612788985 1102 26-27 0.06896502423739292 1102 28-29 0.21091368176563918 1102 30-31 0.3970403815939605 1102 32-33 0.38449142641960776 1102 34-35 0.2731626312803641 1102 36-37 0.3356528084004964 1102 38-39 0.48012438735986507 1102 40-41 0.4864087685013416 1102 42-43 0.40554892217345895 1102 44-45 0.5027157269273417 1102 46-47 0.4344795932051184 1102 48-49 0.44812712948470335 1102 50-51 0.21305049680809418 1102 52-53 0.228225101231061 1102 54-55 0.4406165733948839 1102 56-57 0.3193796287798065 1102 58-59 0.09630174698521898 1102 60-61 0.3068712922851873 1102 62-63 0.21036047630421706 1102 64-65 0.3407883426045899 1102 66-67 0.46699397388627517 1102 68-69 0.37644741972841445 1102 70-71 0.3828360195068221 1102 72-73 0.4847399231923575 1102 74-75 0.29693579914829726 1102 76-77 0.16133627095885927 1102 78-79 0.32576654888545775 1102 80-81 0.6326403727792638 1102 82-83 0.6202699695053973 1102 84-85 0.7032721755714633 1102 86-87 0.6146336375259587 1102 88-89 0.5219570674539966 1102 90-91 0.5564373845422494 1102 92-93 0.49858130277736734 1102 94-95 0.6265483038519442 1102 96-97 0.8352604950484519 1102 98-99 0.6734917929533779 1102 100-101 1.1253676657753182 1103 1 0.14728176234291723 1103 2 0.1874905729810834 1103 3 0.21017924324515747 1103 4 0.2052217064587296 1103 5 0.030081750473023305 1103 6 0.13611622074544982 1103 7 0.12906977757076987 1103 8 0.14877792287785496 1103 9 0.16738348626451227 1103 10-11 0.17428926453887073 1103 12-13 0.15420395892565608 1103 14-15 0.20295828741055288 1103 16-17 0.2453072825714102 1103 18-19 0.22422264919583768 1103 20-21 0.17070912534816074 1103 22-23 0.18093603448801332 1103 24-25 0.23312932178858148 1103 26-27 0.30477425385715406 1103 28-29 0.32507540770517096 1103 30-31 0.11256226065077612 1103 32-33 0.1791706490922209 1103 34-35 0.24504046586569928 1103 36-37 0.26805231584857836 1103 38-39 0.3924850967346458 1103 40-41 0.38860289732580355 1103 42-43 0.13234271261572417 1103 44-45 0.25114711253843325 1103 46-47 0.2956266405526904 1103 48-49 0.24045182114060282 1103 50-51 0.3245154655552298 1103 52-53 0.2831410197677968 1103 54-55 0.12845517251423644 1103 56-57 0.09008949058499383 1103 58-59 0.14115175619397746 1103 60-61 -0.0035003254089929214 1103 62-63 0.1722409774173812 1103 64-65 -0.019364774685868724 1103 66-67 0.18888752960318556 1103 68-69 0.27711272682042676 1103 70-71 0.3592618794900453 1103 72-73 0.17875501435906216 1103 74-75 0.10767921927160629 1103 76-77 -0.024126244731949242 1103 78-79 0.03213397104346072 1103 80-81 0.23018817481579035 1103 82-83 0.4288353831279039 1103 84-85 0.3169057274705125 1103 86-87 0.18842013877459962 1103 88-89 0.32219121434889075 1103 90-91 0.2439313316733447 1103 92-93 0.3528423078960188 1103 94-95 0.5165810877760499 1103 96-97 0.6270521610386908 1103 98-99 0.9463262000819803 1103 100-101 1.329673240535218 1104 1 0.15936299792243958 1104 2 0.23220759958583415 1104 3 0.2127133787831781 1104 4 0.11240695565200554 1104 5 0.1778181101344103 1104 6 0.16716649843857567 1104 7 0.15954225159109114 1104 8 0.16161529880763226 1104 9 0.10415955305267488 1104 10-11 0.19555045221100187 1104 12-13 0.11122768450777443 1104 14-15 0.1530800612803631 1104 16-17 0.28230542865779995 1104 18-19 0.08921059356482886 1104 20-21 0.12478358126909939 1104 22-23 0.1609191748679848 1104 24-25 0.18644647351549537 1104 26-27 0.22885824613496197 1104 28-29 0.28958963184572895 1104 30-31 0.1827426671796175 1104 32-33 0.19578308660724275 1104 34-35 0.12861266768981494 1104 36-37 0.3584272919897771 1104 38-39 0.34314483500945414 1104 40-41 0.207230915307008 1104 42-43 0.1345666390235536 1104 44-45 0.3174255985101766 1104 46-47 0.28596763245555934 1104 48-49 0.28655570189710744 1104 50-51 0.10023900932703356 1104 52-53 0.2012416016538765 1104 54-55 0.28966758976046947 1104 56-57 0.319094823138137 1104 58-59 0.33375706883643375 1104 60-61 0.46976770494835307 1104 62-63 0.40361392632178195 1104 64-65 0.41537026822057044 1104 66-67 0.1563279255822252 1104 68-69 0.3542935641505167 1104 70-71 0.23957370725835503 1104 72-73 0.21380032011156302 1104 74-75 0.34587506803780954 1104 76-77 0.35752069090318983 1104 78-79 0.30426177469836446 1104 80-81 0.48051682639355064 1104 82-83 0.45605482426086397 1104 84-85 0.40015941211039063 1104 86-87 0.4024528001701846 1104 88-89 0.6142556619295476 1104 90-91 0.7127554483785836 1104 92-93 0.6524471202647035 1104 94-95 0.7569021152817967 1104 96-97 1.0411393619442677 1104 98-99 1.156324799888388 1104 100-101 1.4718734639216073 1105 1 0.0935624848868919 1105 2 0.13273810967587707 1105 3 0.11242457032365394 1105 4 0.02717779989215785 1105 5 0.0600005280510274 1105 6 0.1234712813944796 1105 7 0.09273473704167401 1105 8 0.11212299983487384 1105 9 0.012523124196924584 1105 10-11 0.09790896601712973 1105 12-13 0.04562920979073937 1105 14-15 0.09198314208115477 1105 16-17 0.17312879506280154 1105 18-19 0.09603160214719964 1105 20-21 0.1753556332730568 1105 22-23 0.13966580711961285 1105 24-25 0.08728287670883361 1105 26-27 0.08715432939176537 1105 28-29 0.04519663825104914 1105 30-31 0.12549378042847792 1105 32-33 0.2715354932557901 1105 34-35 0.2482731405232883 1105 36-37 0.1149776755612919 1105 38-39 0.15327994547656942 1105 40-41 0.24144681345418206 1105 42-43 0.2186832548320936 1105 44-45 0.3754470159897494 1105 46-47 0.31809013006034803 1105 48-49 0.272528482727715 1105 50-51 0.41011350808592084 1105 52-53 0.3295358452986221 1105 54-55 0.4150679889456512 1105 56-57 0.23585601065557427 1105 58-59 0.17224878898713314 1105 60-61 0.1316772258091632 1105 62-63 0.2785928806635951 1105 64-65 0.21858228758647158 1105 66-67 0.3924551593905363 1105 68-69 0.3421109193838632 1105 70-71 0.206966051849232 1105 72-73 0.3497265612382705 1105 74-75 0.04375618231103218 1105 76-77 -0.09701164887422209 1105 78-79 0.006034076095581042 1105 80-81 0.1258681550790577 1105 82-83 0.19551950069649848 1105 84-85 0.10710212219568405 1105 86-87 0.19010166607948165 1105 88-89 0.1528045731025145 1105 90-91 0.32283008913141487 1105 92-93 0.1498751193020098 1105 94-95 0.06246734937863252 1105 96-97 0.30776224182681844 1105 98-99 0.49315103311079156 1105 100-101 0.9769407267312751 1106 1 0.10766311196305267 1106 2 0.14324332663728967 1106 3 0.15529784543055314 1106 4 0.06858199948104726 1106 5 0.02122571170715304 1106 6 0.04155535990476267 1106 7 -0.008618290357134128 1106 8 0.02710899253199983 1106 9 0.09454746134359482 1106 10-11 0.10980008200453995 1106 12-13 0.14127636453023484 1106 14-15 0.2560013732580373 1106 16-17 0.19874004253671274 1106 18-19 0.12250228221692794 1106 20-21 0.16382853369544392 1106 22-23 0.1735947037951533 1106 24-25 0.03547831978193727 1106 26-27 0.13783171041642106 1106 28-29 0.18100867152164568 1106 30-31 0.2207811686519321 1106 32-33 0.19803921841776884 1106 34-35 0.20165414783483726 1106 36-37 0.015393471288852822 1106 38-39 0.15092359622202878 1106 40-41 0.2540153104662366 1106 42-43 0.29023854248051606 1106 44-45 0.09361817395569716 1106 46-47 0.07917506722262857 1106 48-49 0.09786921145438754 1106 50-51 0.06434327989264688 1106 52-53 0.06707316196774826 1106 54-55 0.09098739708289827 1106 56-57 0.24847594005529317 1106 58-59 0.0792083803034771 1106 60-61 0.1615218878558764 1106 62-63 0.08136970920206466 1106 64-65 0.194582877367651 1106 66-67 0.14847682517542182 1106 68-69 0.17505201932575432 1106 70-71 0.18962579147818914 1106 72-73 0.12743396039002874 1106 74-75 0.05943568828256929 1106 76-77 -0.11904838877205037 1106 78-79 -0.016437107022799324 1106 80-81 0.17458841666435276 1106 82-83 0.2771165934222424 1106 84-85 0.04157726031722575 1106 86-87 0.16290572863097807 1106 88-89 0.10673919412474575 1106 90-91 0.24895687473728145 1106 92-93 0.10489337649864083 1106 94-95 0.27197930545728255 1106 96-97 0.30097238606300536 1106 98-99 0.6049169940638066 1106 100-101 0.8299277949117148 1107 1 0.043970344075756174 1107 2 0.1209847589075963 1107 3 0.09925693261152446 1107 4 0.06697475611088777 1107 5 0.03340402984291302 1107 6 0.09142075287046225 1107 7 0.047710813388384565 1107 8 -0.04244955429098951 1107 9 0.1477360559303662 1107 10-11 0.14579612581213297 1107 12-13 0.06210282277310597 1107 14-15 0.14930672376703313 1107 16-17 0.1053722935458623 1107 18-19 0.04952950918337251 1107 20-21 0.09114427323002872 1107 22-23 0.11340831245374261 1107 24-25 0.1334210997307963 1107 26-27 0.202504198797115 1107 28-29 0.2406923369499978 1107 30-31 0.11763390501834436 1107 32-33 0.1587297830596981 1107 34-35 0.03730233618819767 1107 36-37 0.1021640282113907 1107 38-39 0.1554449130229898 1107 40-41 0.18364365352923784 1107 42-43 -0.04748568196102809 1107 44-45 0.04178497732007003 1107 46-47 0.11865046967675141 1107 48-49 -0.05554790953112132 1107 50-51 -0.009570616635237172 1107 52-53 -0.010431542041516195 1107 54-55 0.028837544233958567 1107 56-57 -0.1503865742175563 1107 58-59 -0.08269895473456046 1107 60-61 0.04637015340323103 1107 62-63 0.04488380514777646 1107 64-65 -0.0018576752127188456 1107 66-67 -0.014104027496614435 1107 68-69 -0.04746016285208299 1107 70-71 -0.2840528241761042 1107 72-73 -0.16455378506276475 1107 74-75 -0.09216447897109603 1107 76-77 -0.2185931050036949 1107 78-79 -0.16198751631181452 1107 80-81 0.10157334775263038 1107 82-83 -0.06906954060583814 1107 84-85 -0.07730374835101372 1107 86-87 0.054324007526460605 1107 88-89 0.0038556766515611685 1107 90-91 -0.007306614109790388 1107 92-93 0.014672569499719401 1107 94-95 0.2278171015686361 1107 96-97 0.4627669856995489 1107 98-99 0.7282554736636868 1107 100-101 1.226060858066056 1108 1 0.04277693616153755 1108 2 0.03789203455670531 1108 3 0.04779346757676706 1108 4 -0.009421044559339009 1108 5 -0.05657930604974837 1108 6 0.06331926675058241 1108 7 0.0028017708509935346 1108 8 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2316 56-57 -1.0641150494090823 2316 58-59 -0.9970168462492381 2316 60-61 -1.0636448033693497 2316 62-63 -1.0609645205187022 2316 64-65 -1.2081622784564132 2316 66-67 -2.033056918285858 2316 68-69 -1.447603436358328 2316 70-71 -1.1155466096122062 2316 72-73 -0.9277423086735546 2316 74-75 -0.8798841010049863 2316 76-77 -0.9277546438372504 2316 78-79 -1.242898464128821 2316 80-81 -1.6878784292797988 2316 82-83 -1.2684960198541155 2316 84-85 -1.1867801404047924 2316 86-87 -1.5411996644287704 2316 88-89 -1.2350432047553852 2316 90-91 -1.7060970926980659 2316 92-93 -1.4734448685466717 2316 94-95 -1.5963410732357097 2316 96-97 -1.5621179403202525 2316 98-99 -1.7379342547389598 2316 100-101 -1.7337068105550166 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 16.0 4 19.0 5 66.0 6 133.0 7 310.0 8 781.0 9 1427.0 10 2355.0 11 3669.0 12 5238.0 13 7431.0 14 9723.0 15 12629.0 16 15828.0 17 19495.0 18 17774.0 19 13406.0 20 12866.0 21 13814.0 22 14996.0 23 16909.0 24 19778.0 25 23845.0 26 29826.0 27 37137.0 28 45989.0 29 57427.0 30 71612.0 31 88775.0 32 110621.0 33 140113.0 34 184571.0 35 250817.0 36 357347.0 37 454920.0 38 268406.0 39 15159.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.04568556091387 18.95541196296728 14.770623532099455 22.228278944019397 2 15.551022356755864 21.442073332017245 38.013710560847876 24.99319375037902 3 21.80208953375149 23.55688672201597 29.440904352134435 25.200119392098102 4 13.086146738763475 16.31729992211446 37.6750113000692 32.921542039052866 5 13.377048306091396 38.25768294875224 33.940875979587254 14.424392765569106 6 31.877917998438 36.406556959673566 16.555781292469028 15.15974374941941 7 28.691324596416095 32.645515775005386 20.367628306717318 18.2955313218612 8 26.622367087284736 32.45086828007048 19.31779622566207 21.608968406982708 9 25.392397703071403 17.94380851129713 21.035381164222137 35.628412621409325 10-11 25.64329452120188 25.714040575980402 26.807013363908244 21.83565153890947 12-13 27.67596375207562 23.30915199450888 26.732156120159523 22.282728133255976 14-15 23.229703901984195 25.23791747377403 26.79343413143779 24.738944492803984 16-17 22.121976158706847 28.351527363082447 27.010290656479587 22.516205821731123 18-19 21.797681002602324 28.303018756432163 28.36724898923934 21.532051251726177 20-21 23.36520402936657 25.705446646330323 27.980469880601007 22.948879443702104 22-23 22.428426218671856 25.962285914083722 28.45047459246075 23.158813274783668 24-25 23.64470814350277 26.07810177964609 28.192341378271507 22.084848698579634 26-27 22.94337115184462 25.322432197631112 29.662557953499576 22.071638697024696 28-29 21.788727399953594 27.848680516624096 28.489288894594655 21.873303188827652 30-31 22.405207636932815 25.91608766641845 28.19139682792946 23.487307868719277 32-33 22.467749818888066 26.280852194814297 28.618136911375462 22.633261074922174 34-35 23.87063767633484 25.806057587553994 28.20067172720082 22.12263300891034 36-37 23.224898930781322 25.302411962414872 29.20148274280322 22.271206364000584 38-39 22.453660196133164 26.513739686794235 28.97099549988753 22.061604617185072 40-41 22.736164448897114 26.74051418880082 28.18791874149131 22.335402620810758 42-43 22.193754125962545 26.877751089684182 27.26898372838641 23.659511055966867 44-45 23.04438496096253 25.587088777663187 27.6375772943598 23.730948967014477 46-47 23.97452551437418 26.858994718153507 27.378470546013006 21.78800922145931 48-49 23.587618943378747 26.548529844678782 27.43948028858425 22.424370923358218 50-51 21.82025162012144 26.98395393279278 28.706915478441424 22.488878968644357 52-53 23.282074307846177 27.67322903475322 26.513412136800547 22.531284520600057 54-55 22.372149133181658 26.44610931673813 27.410275377714317 23.77146617236589 56-57 23.49346396093214 26.259652457761423 27.07657670249894 23.17030687880749 58-59 24.733577082608036 26.038774670690113 26.5878448918557 22.639803354846148 60-61 23.1033797592915 26.667773411963857 28.514224544284723 21.71462228445992 62-63 22.47444858665116 26.801049926111215 28.136316639685933 22.58818484755169 64-65 22.15289940095448 28.08585000120428 27.215603914268705 22.545646683572524 66-67 22.890287941120242 26.719424655360278 26.482987838825572 23.90729956469391 68-69 22.48477460218064 26.671267097593287 27.2529513757102 23.591006924515874 70-71 23.729564352024276 26.519830760536827 26.835133445093078 22.91547144234582 72-73 23.296569312615315 25.681019615601308 28.368326889415492 22.654084182367892 74-75 22.28268361114773 28.332724394629267 26.847852991086125 22.536739003136876 76-77 22.85518356308959 26.488512832224064 26.823196382733993 23.83310722195235 78-79 22.983097864653892 26.761886329914198 27.1909984302088 23.064017375223102 80-81 23.912749896886552 26.51331614990953 26.776852181983728 22.79708177122019 82-83 23.323943116792073 26.190403485419 27.875976026992213 22.609677370796714 84-85 22.669591849286288 27.15217902736961 27.431909292386514 22.74631983095759 86-87 22.800702149607414 27.319258085139186 26.78402276330664 23.09601700194676 88-89 22.372368949627145 27.519933144718628 25.94001261063336 24.167685295020863 90-91 23.005771545551383 26.099248561414797 26.680897855235436 24.214082037798388 92-93 23.84403293313768 26.78903197837946 26.963382871809515 22.403552216673347 94-95 23.10059552814147 26.356603816592646 27.260196381931074 23.282604273334815 96-97 21.656824812267907 26.54268675421369 28.5351703743589 23.265318059159505 98-99 22.478743124040378 27.380210998954897 26.407211639779284 23.733834237225444 100-101 21.834116915152745 26.318263603045615 27.052077242680838 24.7955422391208 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 163.0 1 135.0 2 144.5 3 174.5 4 159.0 5 161.0 6 252.0 7 510.5 8 850.5 9 1063.0 10 1051.5 11 977.0 12 1035.5 13 1202.5 14 1382.5 15 1570.0 16 1766.0 17 1944.5 18 2170.5 19 2489.5 20 2803.5 21 3127.0 22 3664.0 23 4537.5 24 5557.0 25 6930.5 26 9138.0 27 11772.5 28 14517.0 29 18117.0 30 22596.0 31 27421.0 32 33376.5 33 38176.0 34 41385.0 35 45067.0 36 51153.5 37 56923.0 38 59007.5 39 60303.5 40 62657.5 41 65650.0 42 67297.0 43 69707.5 44 72998.0 45 76470.0 46 80238.0 47 104845.5 48 154950.5 49 150080.5 50 101580.0 51 81090.0 52 75441.0 53 73955.0 54 73006.5 55 68762.0 56 63521.5 57 58207.0 58 53716.0 59 48126.5 60 40450.0 61 33341.5 62 27495.5 63 21581.5 64 15835.5 65 12104.5 66 9204.0 67 7094.5 68 5274.5 69 3729.5 70 2846.5 71 2134.5 72 1581.0 73 1345.5 74 1163.5 75 944.5 76 710.0 77 450.0 78 276.0 79 197.5 80 157.0 81 122.0 82 79.0 83 45.0 84 24.0 85 12.5 86 11.5 87 9.5 88 5.0 89 4.0 90 2.5 91 0.5 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.038963904200403486 2 0.006881042684397967 3 0.005031762462966013 4 9.461433691047204E-4 5 6.450977516623094E-4 6 2.1503258388743646E-4 7 0.0 8 0.0 9 0.0015912411207670298 10-11 4.730716845523602E-4 12-13 8.601303355497458E-4 14-15 0.0022793453892068264 16-17 0.0026449007818154686 18-19 0.0 20-21 0.0 22-23 0.0010106531442709513 24-25 8.386270771610023E-4 26-27 0.0038275799931963687 28-29 0.004107122352250036 30-31 0.001225685728158388 32-33 4.085619093861293E-4 34-35 0.007655159986392737 36-37 0.009181891331993536 38-39 0.007569146952837763 40-41 0.0013977117952683371 42-43 6.020912348848221E-4 44-45 0.001161175952992157 46-47 2.1503258388743646E-5 48-49 6.66601010051053E-4 50-51 7.526140436060276E-4 52-53 0.0033330050502552657 54-55 0.00253738448987175 56-57 0.0018922867382094408 58-59 0.0010966661778259261 60-61 0.003999606060306318 62-63 0.004859736395856064 64-65 0.008472283805164996 66-67 0.0035910441509201894 68-69 0.0036340506676976764 70-71 1.2901955033246189E-4 72-73 0.0016772541543220046 74-75 0.0062574481911244016 76-77 0.002042809546930646 78-79 0.0033975148254214963 80-81 0.005160782013298475 82-83 0.004902742912633552 84-85 0.005676860214628323 86-87 0.0035910441509201894 88-89 0.00890234897293987 90-91 0.012407380090305085 92-93 0.0014192150536570807 94-95 0.0028384301073141613 96-97 0.0017417639294882354 98-99 0.004257645160971242 100-101 0.0049672526877997825 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2325229.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.96312580443026 #Duplication Level Percentage of deduplicated Percentage of total 1 74.97422243001154 37.459465073600036 2 14.087564021036354 14.077174669220094 3 4.6419256448694135 6.957753449082647 4 2.07972012784869 4.156372735428395 5 1.1484736482806024 2.869066668605836 6 0.6800643514573833 2.038688444818411 7 0.4447856247534531 1.555601608789122 8 0.31678662963014587 1.2662120183497947 9 0.22391123251720388 1.0068574571353885 >10 1.2515050569263313 11.538645445476268 >50 0.09711144996799956 3.3455017282055155 >100 0.04787370041885421 4.274739705133032 >500 0.0026819792963158296 0.8753230312841198 >1k 0.002162886529286929 2.240351475971562 >5k 8.651546117147718E-4 3.0937590121020406 >10k+ 3.460618446859086E-4 3.244487476797729 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 34462 1.482090581185767 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 16191 0.6963185131442968 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 12623 0.5428712612822221 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 11783 0.5067457871891328 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 9396 0.4040892316412706 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 9098 0.39127328964157937 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 8781 0.3776402238231159 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8129 0.3495999748841942 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 7182 0.30887280349591373 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 6456 0.27765007231545796 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6429 0.2764888963624658 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5735 0.24664237371888964 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5251 0.22582721959858576 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5115 0.2199783333168475 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4240 0.18234763113654612 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3634 0.1562856819693888 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3471 0.1492756197346584 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3439 0.1478994111977788 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3184 0.13693274941951955 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3021 0.1299226871847891 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2839 0.12209550113128642 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2806 0.12067628607762934 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2732 0.11749380383609528 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2635 0.11332217170867902 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.2901955033246189E-4 0.0 5 0.0 0.0 0.0 1.2901955033246189E-4 0.0 6 0.0 0.0 0.0 1.2901955033246189E-4 0.0 7 0.0 0.0 0.0 1.2901955033246189E-4 0.0 8 0.0 0.0 0.0 1.2901955033246189E-4 0.0 9 0.0 0.0 0.0 1.2901955033246189E-4 0.0 10-11 0.0 0.0 0.0 1.7202606710994917E-4 0.0 12-13 0.0 0.0 0.0 1.7202606710994917E-4 0.0 14-15 0.0 0.0 0.0 1.7202606710994917E-4 0.0 16-17 0.0 0.0 0.0 2.1503258388743646E-4 0.0 18-19 0.0 0.0 0.0 2.795423590536674E-4 0.0 20-21 0.0 0.0 0.0 3.65555392608642E-4 0.0 22-23 0.0 0.0 0.0 3.8705865099738563E-4 0.0 24-25 0.0 0.0 0.0 4.730716845523602E-4 0.0 26-27 0.0 0.0 0.0 5.375814597185912E-4 0.0 28-29 0.0 0.0 0.0 8.386270771610022E-4 0.0 30-31 0.0 0.0 0.0 0.0014837248288233117 0.0 32-33 0.0 0.0 0.0 0.002236338872429339 0.0 34-35 0.0 0.0 0.0 0.0033545083086440088 0.0 36-37 0.0 0.0 0.0 0.00436516145291496 0.0 38-39 0.0 0.0 0.0 0.005440324372352143 0.0 40-41 0.0 0.0 0.0 0.00808522515416761 0.0 42-43 0.0 0.0 0.0 0.011224700878924184 0.0 44-45 0.0 0.0 0.0 0.018858357606928177 0.0 46-47 0.0 0.0 0.0 0.03474926555620973 0.0 48-49 0.0 0.0 0.0 0.045629914300914017 0.0 50-51 0.0 2.1503258388743646E-5 0.0 0.05977905832070734 0.0 52-53 0.0 4.300651677748729E-5 0.0 0.07551944346126768 0.0 54-55 0.0 4.300651677748729E-5 0.0 0.09461433691047205 0.0 56-57 0.0 4.300651677748729E-5 0.0 0.12078380236957306 0.0 58-59 0.0 4.300651677748729E-5 0.0 0.15471594410701053 0.0 60-61 0.0 4.300651677748729E-5 0.0 0.19694834358250304 0.0 62-63 0.0 4.300651677748729E-5 0.0 0.25335139033617765 0.0 64-65 0.0 6.450977516623094E-5 0.0 0.29444411711706675 0.0 66-67 0.0 8.601303355497459E-5 0.0 0.3677057184475163 0.0 68-69 0.0 8.601303355497459E-5 0.0 0.46128789895532873 0.0 70-71 0.0 8.601303355497459E-5 0.0 0.5196692454807676 0.0 72-73 0.0 8.601303355497459E-5 0.0 0.6187992666528759 0.0 74-75 0.0 8.601303355497459E-5 0.0 0.703436091670971 0.0 76-77 0.0 8.601303355497459E-5 0.0 0.773214165142444 0.0 78-79 0.0 8.601303355497459E-5 0.0 0.8712905266535038 0.0 80-81 0.0 8.601303355497459E-5 0.0 0.973689043100701 0.0 82-83 0.0 8.601303355497459E-5 0.0 1.1036332335438788 0.0 84-85 0.0 8.601303355497459E-5 0.0 1.2932274627574316 2.1503258388743646E-5 86-87 0.0 8.601303355497459E-5 0.0 1.5509010080297467 4.300651677748729E-5 88-89 0.0 8.601303355497459E-5 0.0 1.8754281836326658 4.300651677748729E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10105 0.0 24.171839 1 GTACCGT 120 1.7117341E-4 23.749622 6 TACATGG 10420 0.0 22.749697 2 ACATGGG 10200 0.0 22.539827 3 GAGTACT 7160 0.0 21.462057 12-13 GTAAGGT 965 0.0 20.67236 4 GTATAGG 720 0.0 20.460304 1 CATGGGG 5165 0.0 20.231142 4 CGCGGAA 235 0.0 20.211794 82-83 TACCGTG 170 7.959288E-5 19.558516 7 GTACTTT 7835 0.0 19.552221 14-15 GGTAAGG 1070 0.0 19.533241 3 TAAGGTG 1170 0.0 19.080484 5 CTCGCGC 250 0.0 18.999903 60-61 AGTACTT 7745 0.0 18.706327 12-13 AGAGTAC 13415 0.0 18.21876 10-11 CATGGGA 5045 0.0 17.699652 4 TACCTGG 1535 0.0 17.640432 2 CGATCGT 285 0.0 17.500475 24-25 ACTTTTT 9045 0.0 17.1979 16-17 >>END_MODULE