FastQCFastQC Report
Thu 26 May 2016
SRR936504_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936504_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2396805
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT305461.2744466070456295No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT189390.7901769230287822No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT137870.5752241004170134No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC120290.5018764563658704No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT94100.3926059900575975No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT86110.35926994478065594No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC77010.3213027342649903No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC70740.2951429089976031No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC65300.2724460271069194No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG61080.2548392547578964No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA57980.24190536985695543No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA47680.19893149421834483No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44380.1851631651302463No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT38230.1595040063751536No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA37330.1557490075329449No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC36060.15045028694449486No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35730.149073454035685No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC34380.14344095577237195No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG31550.13163357052409355No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC28580.11924207434480485No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC27090.11302546515048158No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG26520.11064729921708273No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT26420.110230077123504No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGG300.00395457931.6627666-67
GTACACG2850.026.6820321
GTACATG105650.024.8320941
TACATGG109050.023.6200682
ACATGGG108750.023.207923
GTAAGCG3650.022.77496594-95
GTATTCG1353.782861E-421.1232761
GAGTACT58400.020.5163112-13
CATGGGG55150.020.5125924
TACACCG3003.6907295E-919.0129325
GTACTTT63400.018.85608314-15
AGTACTT65250.018.21675712-13
AGAGTAC121500.018.0021710-11
CATGGGA54200.017.5396064
ACCGTCG1700.001775734416.7824258
GAGTACA86300.016.4666081
GTGTAGC13700.016.305011
ACTTTTT75500.016.27210616-17
GTAAGGT8000.016.0421644
ATACCGT2103.983996E-415.8444416
Produced by FastQC (version 0.11.5)