##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936504_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2396805 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.322116734569562 31.0 31.0 34.0 28.0 34.0 2 31.360335530007657 31.0 31.0 34.0 28.0 34.0 3 31.316370751896795 31.0 31.0 34.0 28.0 34.0 4 34.95263611349275 37.0 35.0 37.0 32.0 37.0 5 35.005045049555555 37.0 35.0 37.0 32.0 37.0 6 35.07816322145523 37.0 35.0 37.0 32.0 37.0 7 34.939356351476235 37.0 35.0 37.0 32.0 37.0 8 34.92649923544052 37.0 35.0 37.0 32.0 37.0 9 36.40236773538106 39.0 37.0 39.0 32.0 39.0 10-11 36.269250731703245 39.0 35.0 39.0 32.0 39.0 12-13 36.187066949543244 39.0 35.0 39.0 31.5 39.0 14-15 37.156150792409065 39.0 36.5 41.0 31.0 41.0 16-17 37.10133511069945 39.0 36.0 41.0 31.0 41.0 18-19 36.978772157100806 39.0 36.0 41.0 31.0 41.0 20-21 36.93377642319672 39.0 36.0 41.0 30.5 41.0 22-23 36.94538833989415 39.0 36.0 41.0 30.5 41.0 24-25 37.05225435527713 39.0 36.0 41.0 31.0 41.0 26-27 36.98331236792313 39.0 36.0 41.0 30.5 41.0 28-29 36.90417347260207 39.0 36.0 41.0 30.0 41.0 30-31 36.76133519414387 39.0 36.0 41.0 30.0 41.0 32-33 36.60765811152764 39.0 36.0 41.0 30.0 41.0 34-35 36.39241782289339 39.0 35.0 40.5 30.0 41.0 36-37 36.19291264829638 39.0 35.0 40.0 29.5 41.0 38-39 36.00504713566602 38.0 35.0 40.0 29.0 41.0 40-41 35.84232008861797 38.0 35.0 40.0 28.5 41.0 42-43 35.57287826919587 38.0 35.0 40.0 27.0 41.0 44-45 35.439631509446954 38.0 34.5 40.0 27.0 41.0 46-47 35.33539566214189 38.0 34.0 40.0 26.5 41.0 48-49 35.11066419671187 38.0 34.0 40.0 26.0 41.0 50-51 34.1077970464848 37.5 33.0 39.5 24.0 40.5 52-53 34.24305794589047 37.5 33.5 39.5 24.0 40.5 54-55 34.70588053679795 38.0 34.0 40.0 25.5 41.0 56-57 34.4955428163743 38.0 34.0 40.0 24.5 41.0 58-59 34.24703052605447 38.0 33.0 40.0 23.5 41.0 60-61 33.88151873014283 37.0 33.0 40.0 23.0 41.0 62-63 33.58461243196672 37.0 33.0 40.0 22.0 41.0 64-65 33.24192456207326 36.0 32.0 39.0 22.0 41.0 66-67 32.80473046409699 36.0 32.0 39.0 21.0 41.0 68-69 32.340758843543796 35.0 31.0 39.0 19.5 40.0 70-71 31.881686870646547 35.0 31.0 38.0 18.5 40.0 72-73 31.454877847801555 35.0 31.0 37.0 17.5 39.5 74-75 31.070129401432325 35.0 30.0 37.0 14.5 39.0 76-77 30.629787779982102 34.5 30.0 36.0 11.0 39.0 78-79 30.14435988743348 34.0 29.5 36.0 7.5 37.5 80-81 29.637557707030815 34.0 29.0 35.0 7.0 37.0 82-83 29.229570615882395 34.0 29.0 35.0 2.0 37.0 84-85 28.85454532179297 34.0 29.0 35.0 2.0 36.0 86-87 28.594497675029885 34.0 29.0 35.0 2.0 36.0 88-89 28.22549226991766 34.0 27.5 35.0 2.0 35.5 90-91 27.825331639411637 34.0 27.0 35.0 2.0 35.0 92-93 27.393346559273702 33.0 26.0 35.0 2.0 35.0 94-95 26.88716666562361 33.0 25.0 35.0 2.0 35.0 96-97 26.282570964262842 33.0 25.0 35.0 2.0 35.0 98-99 25.580264977751632 33.0 22.0 35.0 2.0 35.0 100-101 22.986172216763563 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6835358812001537 1101 2 0.696114564439803 1101 3 0.8491597043751895 1101 4 0.8895068600321636 1101 5 0.7333681163349866 1101 6 0.7732234344495552 1101 7 0.7953654424526704 1101 8 0.7484406757212554 1101 9 0.9892321569991367 1101 10-11 0.9714780576716535 1101 12-13 1.0554879981798848 1101 14-15 1.3230344181700175 1101 16-17 1.3745342678147807 1101 18-19 1.5043910949503498 1101 20-21 1.4375949440802458 1101 22-23 1.3208460569414342 1101 24-25 1.1583506081609798 1101 26-27 1.1830491442142872 1101 28-29 1.1955105993742166 1101 30-31 1.0847682762435653 1101 32-33 1.0477580541086198 1101 34-35 0.9585448534299061 1101 36-37 1.0172065616866988 1101 38-39 1.1053898308264039 1101 40-41 0.987983495537172 1101 42-43 1.0501613071892635 1101 44-45 0.989116177236852 1101 46-47 1.0936050992602446 1101 48-49 1.1859383608355216 1101 50-51 1.122312711486046 1101 52-53 1.2110583201579672 1101 54-55 1.2681651185869 1101 56-57 1.3409143215960668 1101 58-59 1.3155240347477601 1101 60-61 1.140377925560145 1101 62-63 1.3548702128728323 1101 64-65 1.110376384458604 1101 66-67 1.1326607789137597 1101 68-69 1.2548369679322278 1101 70-71 1.218589734000929 1101 72-73 1.2410748327796952 1101 74-75 1.1523819635105603 1101 76-77 1.1720356499315976 1101 78-79 1.2313042656599684 1101 80-81 1.2470588000152354 1101 82-83 1.1972250412124978 1101 84-85 1.2284079538848331 1101 86-87 1.3311645010718642 1101 88-89 1.2779817811696397 1101 90-91 1.3807762066443665 1101 92-93 1.4661510215791758 1101 94-95 1.4880346381293492 1101 96-97 1.3570444182370416 1101 98-99 1.3071810279119873 1101 100-101 1.6120514986501782 1102 1 0.41510467265262463 1102 2 0.5227686041332618 1102 3 0.5762488959578107 1102 4 0.40529154685152946 1102 5 0.4840507297303134 1102 6 0.5189102428203611 1102 7 0.5323084600017012 1102 8 0.4367717870436749 1102 9 0.5044999917901976 1102 10-11 0.6575027410755325 1102 12-13 0.5823785348715305 1102 14-15 0.8662452530445321 1102 16-17 0.7285876103078479 1102 18-19 0.7934747143635477 1102 20-21 0.7682139956729443 1102 22-23 0.6406985438794379 1102 24-25 0.8250066014127952 1102 26-27 0.7350448463859109 1102 28-29 0.5205956470843773 1102 30-31 0.5877409839983514 1102 32-33 0.7146605062124749 1102 34-35 0.33275219065871653 1102 36-37 0.40865308108612197 1102 38-39 0.3397547335333755 1102 40-41 0.4607054815427105 1102 42-43 0.4902045312850518 1102 44-45 0.4971697538981701 1102 46-47 0.49165846245326605 1102 48-49 0.8149188168426136 1102 50-51 0.7339330199542076 1102 52-53 0.6313670470653889 1102 54-55 0.7495911126861259 1102 56-57 0.6756927706130469 1102 58-59 0.4683036183810785 1102 60-61 0.6248626616065991 1102 62-63 0.721916012172521 1102 64-65 0.8644376188099159 1102 66-67 0.7858398070120032 1102 68-69 0.7404786374613153 1102 70-71 0.5206903914185972 1102 72-73 0.7620270695716655 1102 74-75 0.7226473175412629 1102 76-77 0.793606815410282 1102 78-79 0.7861009306382947 1102 80-81 1.0359017824390335 1102 82-83 0.7189263576650191 1102 84-85 0.7966783496485874 1102 86-87 0.6943196789394754 1102 88-89 0.8482493992580409 1102 90-91 0.9262740632286004 1102 92-93 0.9395124421654124 1102 94-95 1.0204779380387237 1102 96-97 1.1784057379109925 1102 98-99 1.136825564384985 1102 100-101 1.5672260672016414 1103 1 0.5710732446707425 1103 2 0.6211942000689383 1103 3 0.7562714532676011 1103 4 0.6747143722046616 1103 5 0.6916398821884826 1103 6 0.7456522741401628 1103 7 0.7547906455365805 1103 8 0.6395471775395549 1103 9 0.7836418924939608 1103 10-11 0.7052029371999211 1103 12-13 0.7742853487416639 1103 14-15 1.1462934527668622 1103 16-17 1.301130896903878 1103 18-19 1.3271388949181855 1103 20-21 1.2482706093119091 1103 22-23 1.0404116693890302 1103 24-25 0.9348152248760044 1103 26-27 1.0061695867063989 1103 28-29 0.9616231065022589 1103 30-31 0.944161102874979 1103 32-33 1.0569698859265273 1103 34-35 0.9118340921237333 1103 36-37 1.0291269448567988 1103 38-39 0.8956556937215225 1103 40-41 1.126434397169156 1103 42-43 1.0865145280196487 1103 44-45 1.0749521059083946 1103 46-47 1.1459541675494975 1103 48-49 1.2049085598775946 1103 50-51 1.1262698450629358 1103 52-53 1.2041208819082172 1103 54-55 1.2104978524336687 1103 56-57 1.3095077005070905 1103 58-59 1.1194615335728102 1103 60-61 1.1067361804066849 1103 62-63 1.2473005982779384 1103 64-65 1.2057512515584818 1103 66-67 1.0356874550218151 1103 68-69 1.1613082897061204 1103 70-71 1.1285012105133845 1103 72-73 1.2862922041041855 1103 74-75 1.0471234009832706 1103 76-77 1.1342012586193455 1103 78-79 1.0911190305755945 1103 80-81 1.161944657354141 1103 82-83 1.1075878036967524 1103 84-85 1.1992957788765324 1103 86-87 1.1767963954285499 1103 88-89 1.1950497069669908 1103 90-91 1.4143935127981884 1103 92-93 1.1788157498362999 1103 94-95 1.29813348030563 1103 96-97 1.3736814238956114 1103 98-99 1.5467381598178314 1103 100-101 1.7087404319609512 1104 1 0.43818502300550577 1104 2 0.45992170217310857 1104 3 0.49194386792563094 1104 4 0.5113408630067013 1104 5 0.5263048964856907 1104 6 0.3691675105596701 1104 7 0.4374708655965165 1104 8 0.4954658869077093 1104 9 0.6072150980969084 1104 10-11 0.6343061613641581 1104 12-13 0.6641981534282522 1104 14-15 0.7366780002635025 1104 16-17 0.6941771031738355 1104 18-19 0.6644410425649525 1104 20-21 0.7168498255016544 1104 22-23 0.5672642214265906 1104 24-25 0.6263662163905792 1104 26-27 0.4582105850397866 1104 28-29 0.28010617188294873 1104 30-31 0.5172660029431668 1104 32-33 0.45685803513028134 1104 34-35 0.5541494593455027 1104 36-37 0.4894789961669588 1104 38-39 0.3853257524524807 1104 40-41 0.5320113298933151 1104 42-43 0.6900208275233766 1104 44-45 0.8572682534845626 1104 46-47 0.768264964124107 1104 48-49 0.8890338290534103 1104 50-51 0.659215571414947 1104 52-53 0.7301071476709069 1104 54-55 0.804684212210482 1104 56-57 0.8232545721220745 1104 58-59 0.7420253879289476 1104 60-61 0.7470889563316376 1104 62-63 0.660948439204553 1104 64-65 0.7500002254853584 1104 66-67 0.9323995618486194 1104 68-69 0.7962804291963224 1104 70-71 0.7712898069345258 1104 72-73 0.8903385274273035 1104 74-75 0.8790586612964937 1104 76-77 0.8649958080297147 1104 78-79 0.6581538882631044 1104 80-81 0.8643671003464668 1104 82-83 0.8767130821625031 1104 84-85 0.8745824197667318 1104 86-87 0.7863662343596864 1104 88-89 0.958978289676196 1104 90-91 1.0509249675831533 1104 92-93 1.0820866424316655 1104 94-95 1.036869250450387 1104 96-97 0.8784487389376849 1104 98-99 0.776109955952105 1104 100-101 1.393022329411238 1105 1 0.35009288909095204 1105 2 0.30977202440276486 1105 3 0.48181709174713205 1105 4 0.3881996424768701 1105 5 0.405774107102026 1105 6 0.39860144832834266 1105 7 0.401475559942007 1105 8 0.3804173292134223 1105 9 0.3834488921639263 1105 10-11 0.5434636383148757 1105 12-13 0.5137711355946877 1105 14-15 0.7621932002162524 1105 16-17 0.684484797806455 1105 18-19 0.5616414706474941 1105 20-21 0.6662316576839586 1105 22-23 0.5995440288718754 1105 24-25 0.43735149315363486 1105 26-27 0.3781291140349552 1105 28-29 0.4029849067863722 1105 30-31 0.4024656774850257 1105 32-33 0.5727508915371615 1105 34-35 0.4796995371724577 1105 36-37 0.5834635331275422 1105 38-39 0.31601615233048364 1105 40-41 0.42515065798506413 1105 42-43 0.4070324720280283 1105 44-45 0.4420672532056784 1105 46-47 0.5069706120385788 1105 48-49 0.8133108902310227 1105 50-51 0.6541879885289106 1105 52-53 0.7405183047439508 1105 54-55 0.6699636576540016 1105 56-57 0.557217704955427 1105 58-59 0.6806408445114087 1105 60-61 0.533139558271202 1105 62-63 0.8071204765944628 1105 64-65 0.8410798184772261 1105 66-67 0.7419066933521137 1105 68-69 0.7122081806501726 1105 70-71 0.9473732706663469 1105 72-73 0.765109700409198 1105 74-75 0.7141318241203543 1105 76-77 0.8045308658744368 1105 78-79 0.9185905468837596 1105 80-81 0.8864182112866139 1105 82-83 0.8187290885480216 1105 84-85 0.8864469074621013 1105 86-87 0.8633629626055743 1105 88-89 0.9020517087176252 1105 90-91 0.9726250844953022 1105 92-93 0.9939417022821679 1105 94-95 0.9785339021799615 1105 96-97 0.9692965855423559 1105 98-99 1.3357629700461295 1105 100-101 1.5962413288026376 1106 1 0.30596837680265665 1106 2 0.22768457228628947 1106 3 0.17214826886982237 1106 4 0.4058997801605031 1106 5 0.4686141245130173 1106 6 0.23603071615915638 1106 7 0.4810061008532429 1106 8 0.5721322465177678 1106 9 0.576749406735118 1106 10-11 0.6632069707846711 1106 12-13 0.6122801876027992 1106 14-15 0.5434201460988248 1106 16-17 0.525955316809295 1106 18-19 0.6579582034820746 1106 20-21 0.35729851183004513 1106 22-23 0.32638980479867286 1106 24-25 0.09745868123841461 1106 26-27 0.21210125052454032 1106 28-29 0.1948880870546148 1106 30-31 0.26293007699636206 1106 32-33 0.37079131621290884 1106 34-35 0.43694413535850174 1106 36-37 0.17234516926141197 1106 38-39 0.3685512669156097 1106 40-41 0.3514148783893276 1106 42-43 0.2861235299564058 1106 44-45 0.23904759942674048 1106 46-47 0.25372495393845185 1106 48-49 0.2803496665168268 1106 50-51 0.2083743696850533 1106 52-53 0.33263581844327916 1106 54-55 0.40280523008781444 1106 56-57 0.4804443707366417 1106 58-59 0.3599331677615254 1106 60-61 0.5124658092919958 1106 62-63 0.3995712681766932 1106 64-65 0.20937374146118515 1106 66-67 0.11417233530367099 1106 68-69 0.3625155195688805 1106 70-71 0.19387578434594843 1106 72-73 0.23990953642496748 1106 74-75 0.46107963978998967 1106 76-77 0.283394719516874 1106 78-79 0.34552493938598516 1106 80-81 0.44751178683968007 1106 82-83 0.38333009533489104 1106 84-85 0.5339414796763116 1106 86-87 0.7363983901678104 1106 88-89 0.835505690562325 1106 90-91 1.0260833500498059 1106 92-93 0.9835385831628152 1106 94-95 1.0900180251668132 1106 96-97 1.0302237276273871 1106 98-99 0.9843662768202428 1106 100-101 1.6079418070789977 1107 1 0.33205622817251523 1107 2 0.3128517970284257 1107 3 0.3569866644622124 1107 4 0.3517153223644627 1107 5 0.40681134284322695 1107 6 0.4337528183225956 1107 7 0.3856535633909459 1107 8 0.5002589336076255 1107 9 0.525609587060778 1107 10-11 0.534197495375615 1107 12-13 0.42813435971169866 1107 14-15 0.41646620909406806 1107 16-17 0.3633421778920436 1107 18-19 0.6617851763382347 1107 20-21 0.4142825294984789 1107 22-23 0.5348627361593472 1107 24-25 0.2986780563522089 1107 26-27 0.21020949341798456 1107 28-29 0.3580297748964796 1107 30-31 0.2655060108822269 1107 32-33 0.3195975096632466 1107 34-35 0.4274944531844582 1107 36-37 0.509785146463436 1107 38-39 0.557784051672634 1107 40-41 0.4653468960894216 1107 42-43 0.3539102263871001 1107 44-45 0.40844091993621134 1107 46-47 0.3836893320998982 1107 48-49 0.5138314241774964 1107 50-51 0.3625754510057533 1107 52-53 0.40583030576586765 1107 54-55 0.4777389338882756 1107 56-57 0.6828246219893401 1107 58-59 0.698177090280069 1107 60-61 0.5318765449225396 1107 62-63 0.5521075430822933 1107 64-65 0.6610764653444363 1107 66-67 0.5223938556437346 1107 68-69 0.5394399300274486 1107 70-71 0.46220337860528815 1107 72-73 0.5713738314687831 1107 74-75 0.515996165948291 1107 76-77 0.5718362244999717 1107 78-79 0.7046138910748567 1107 80-81 0.6799458666484313 1107 82-83 0.6515128274507482 1107 84-85 0.8550433818824921 1107 86-87 0.9038638567743185 1107 88-89 0.978644766136032 1107 90-91 0.7867542280093822 1107 92-93 0.8746281364649597 1107 94-95 0.8856345600326776 1107 96-97 1.0724367145581297 1107 98-99 1.3399272416179535 1107 100-101 1.7260324765320867 1108 1 0.10027014326501416 1108 2 0.11124540516806292 1108 3 0.15341371439081897 1108 4 0.20378838557303425 1108 5 0.0756302687422803 1108 6 0.2818902682886488 1108 7 0.21524088578507872 1108 8 0.2691490188896495 1108 9 -0.13981288276280424 1108 10-11 0.1732448023206743 1108 12-13 0.19131257807424618 1108 14-15 0.5481851139261948 1108 16-17 0.5530043268741949 1108 18-19 0.41241484324678623 1108 20-21 0.4101449859016597 1108 22-23 0.4654437930946571 1108 24-25 0.41177731276786034 1108 26-27 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2315 90-91 -2.129614038121531 2315 92-93 -2.1249574356510976 2315 94-95 -2.3731990925705375 2315 96-97 -2.3396726470578386 2315 98-99 -2.4975589363321795 2315 100-101 -2.8403979304202096 2316 1 -5.070281429654123 2316 2 -5.0267570881554064 2316 3 -5.91421742036038 2316 4 -7.668718863710321 2316 5 -8.551407452203577 2316 6 -9.436942747769212 2316 7 -11.444836719524702 2316 8 -11.215365165456273 2316 9 -11.145916837503734 2316 10-11 -11.287225940251957 2316 12-13 -9.943868801162928 2316 14-15 -9.882533078840769 2316 16-17 -8.871745961881139 2316 18-19 -9.16058756372324 2316 20-21 -10.416584453255648 2316 22-23 -9.888985715533106 2316 24-25 -9.706273922667922 2316 26-27 -7.632906746877957 2316 28-29 -5.988253592303362 2316 30-31 -8.191102648524843 2316 32-33 -5.888007346273817 2316 34-35 -4.373821998994288 2316 36-37 -4.887404974934995 2316 38-39 -4.067733411291293 2316 40-41 -3.719036820196031 2316 42-43 -3.6392831253557247 2316 44-45 -3.8771432340088055 2316 46-47 -3.9990330145890027 2316 48-49 -4.6466368020855455 2316 50-51 -4.648197052858503 2316 52-53 -4.671322475630134 2316 54-55 -3.9980994794221374 2316 56-57 -4.379479864021182 2316 58-59 -4.729777198609973 2316 60-61 -5.0302676395891766 2316 62-63 -4.6778877677081 2316 64-65 -5.423941509959057 2316 66-67 -4.883788085689041 2316 68-69 -4.960988351116718 2316 70-71 -4.634382008428176 2316 72-73 -3.782766219591501 2316 74-75 -4.222760672482384 2316 76-77 -4.64910196477042 2316 78-79 -4.905595733962048 2316 80-81 -5.5749065969233556 2316 82-83 -4.935579515243639 2316 84-85 -4.890075150915926 2316 86-87 -5.388761405908255 2316 88-89 -7.1073702740695985 2316 90-91 -6.150237383515648 2316 92-93 -5.452836564207157 2316 94-95 -5.100844017226393 2316 96-97 -6.428779527474553 2316 98-99 -8.250815911267647 2316 100-101 -8.769336147445404 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5454.0 3 2096.0 4 2185.0 5 2329.0 6 2647.0 7 3049.0 8 3847.0 9 4999.0 10 6222.0 11 7854.0 12 9598.0 13 11162.0 14 12657.0 15 14694.0 16 17081.0 17 20540.0 18 17524.0 19 15432.0 20 16545.0 21 18255.0 22 20310.0 23 23172.0 24 26353.0 25 30189.0 26 34977.0 27 41648.0 28 49364.0 29 59251.0 30 71815.0 31 87493.0 32 107951.0 33 136681.0 34 177320.0 35 237462.0 36 325850.0 37 415371.0 38 312898.0 39 44519.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.27185719850905 18.252025374646816 14.108457283102343 22.367660143741787 2 15.337586157245973 20.774272710768326 38.7476915179326 25.1404496140531 3 21.53010417240441 23.68006836887814 29.13443097430659 25.655396484410858 4 12.642834906176109 15.77086823091285 38.118965170442074 33.46733169246897 5 13.492352641926528 38.17894847105004 33.79898981173005 14.529709075293374 6 31.714701158904468 35.94256561616256 16.956657106511702 15.386076118421272 7 28.71953666206118 32.56000390819255 20.428406380207846 18.292053049538428 8 26.326500368399852 32.36925979807931 19.626632491405744 21.677607342115095 9 25.524660778859005 18.06144078853702 21.28779405115153 35.12610438145244 10-11 25.63713612498998 25.587602198947284 26.856593335025515 21.918668341037222 12-13 27.572869025146897 23.273343496857553 26.77566889867676 22.37811857931879 14-15 23.314066314887395 25.080239819905486 26.685203188637985 24.920490676569138 16-17 22.175000083492804 28.39329369094971 26.724961342831477 22.706744882726007 18-19 21.950550058380955 28.344068165243357 27.873430424805456 21.831951351570233 20-21 23.541603115074736 26.01522233633109 27.276141560466826 23.167032988127346 22-23 22.558104502561697 26.17594383043642 27.922709265090397 23.34324240191149 24-25 23.783756263196455 26.212602616473596 27.574715476180288 22.428925644149658 26-27 22.955268335525357 25.59375024126858 29.150148245849607 22.300833177356456 28-29 21.944602014612542 28.041576549163942 27.848994394025468 22.16482704219804 30-31 22.46992477203283 26.209251865470424 27.61490774190305 23.7059156205937 32-33 22.537894395783294 26.52756876883819 28.052315786596754 22.882221048781766 34-35 23.977194441442407 26.135009874730912 27.48680274447835 22.40099293934833 36-37 23.256428171026243 25.551962878904362 28.711874866091726 22.479734083977675 38-39 22.62286059539894 26.814170037445056 28.297180396381844 22.26578897077416 40-41 22.791915925626515 27.080793803948154 27.681810832659657 22.445479437765666 42-43 22.256572035584917 27.066426730241755 26.66196167637288 24.015039557800453 44-45 23.031330035449503 25.97810529460347 27.0879441018913 23.902620568055728 46-47 24.022807996191574 26.992069813346266 26.964741606589808 22.020380583872353 48-49 23.51568381901509 26.685763077254933 27.055925530257085 22.742627573472888 50-51 21.784582635610334 27.158303134887596 28.437496153700927 22.61961807580114 52-53 23.171354997200435 27.812852292346218 26.20890252928694 22.806890181166402 54-55 22.311331775700936 26.55753504672897 27.104556074766357 24.02657710280374 56-57 23.44292411578568 26.38650830473851 26.715814718880598 23.454752860595217 58-59 24.657275902260448 26.206812528724193 26.344104528007378 22.79180704100798 60-61 23.133167853523247 26.718522014113766 28.227784669292816 21.920525463070174 62-63 22.344086523978763 27.08966657292846 27.81865495968284 22.74759194340994 64-65 22.109951666594 28.31224853079505 26.80571239151373 22.772087411097218 66-67 22.780941032454617 26.961902842673524 26.082978379860087 24.17417774501177 68-69 22.391331286917783 26.842055633741406 27.030125868325385 23.73648721101543 70-71 23.757519595383393 26.56466831168029 26.509384838893528 23.168427254042786 72-73 23.125676570080767 25.922242744790047 28.15238882967669 22.799691855452497 74-75 22.14419545985645 28.436425471540645 26.704452512101483 22.714926556501418 76-77 22.702017720180894 26.69781188463624 26.637591397526368 23.962578997656493 78-79 22.95333275049435 26.95109348034786 26.94827665711103 23.14729711204676 80-81 23.828490035741847 26.786857698789124 26.45161747981058 22.933034785658446 82-83 23.208033058844343 26.388161035024883 27.72957020832012 22.67423569781065 84-85 22.69460224635373 27.3661576603274 27.125600584296468 22.8136395090224 86-87 22.746272741818828 27.659972166690277 26.675313030858007 22.91844206063289 88-89 22.38285833017629 27.57429208480129 25.776570497789514 24.26627908723291 90-91 22.97428196437032 26.398116852215754 26.45978689620051 24.167814287213417 92-93 23.89454309812092 27.029725734109505 26.786124847332175 22.289606320437397 94-95 23.068866326456735 26.668027005649787 27.043810217689707 23.219296450203768 96-97 21.684510304035552 26.845593336149925 28.37623421534417 23.09366214447035 98-99 22.51812413719425 27.67784857352232 26.215731901319227 23.5882953879642 100-101 22.041259863023534 26.5300101321858 26.969141067735414 24.45958893705526 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 136.0 1 105.5 2 101.5 3 139.5 4 143.5 5 150.5 6 260.5 7 480.5 8 713.5 9 850.0 10 869.0 11 868.0 12 932.5 13 1070.0 14 1260.0 15 1398.5 16 1520.0 17 1681.5 18 1853.0 19 2066.0 20 2294.0 21 2648.0 22 3224.0 23 3974.0 24 4937.0 25 6329.0 26 8557.5 27 11415.0 28 14077.5 29 17688.5 30 22726.0 31 27845.0 32 33902.0 33 38855.0 34 42679.5 35 46997.0 36 52952.0 37 58607.5 38 60772.5 39 62315.5 40 64733.5 41 67951.0 42 69924.0 43 72822.0 44 76595.0 45 81010.5 46 86129.0 47 108919.5 48 154090.5 49 150689.5 50 106848.0 51 86095.0 52 79533.5 53 77219.0 54 75715.5 55 71441.0 56 65969.5 57 60304.0 58 55773.0 59 49886.0 60 41551.0 61 34208.0 62 28427.5 63 22623.5 64 16822.0 65 12863.5 66 9704.5 67 7449.0 68 5574.0 69 3974.0 70 2992.5 71 2294.0 72 1764.0 73 1416.5 74 1205.5 75 969.5 76 677.5 77 419.0 78 283.5 79 192.5 80 125.0 81 88.5 82 58.5 83 36.0 84 29.0 85 21.0 86 12.0 87 8.5 88 8.0 89 6.5 90 5.0 91 5.5 92 4.5 93 3.0 94 2.5 95 1.0 96 1.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0757675321938998 2 0.03129165701840575 3 0.016396828277644614 4 0.02115316014444229 5 0.025784325383166336 6 0.03124993480904788 7 0.07660197638105729 8 0.054656094258815374 9 0.07034364497737614 10-11 0.061623703221580395 12-13 0.04099207069411154 14-15 0.04898187378614447 16-17 0.05795214879808745 18-19 0.05557398286468862 20-21 0.01910877188590645 22-23 0.014477606647182395 24-25 0.023552187182520064 26-27 0.025721742069129527 28-29 0.006905025648728203 30-31 9.80471919910047E-4 32-33 0.0101593579786424 34-35 0.011849107457636312 36-37 0.0045268597153293655 38-39 0.009470941524237475 40-41 0.004902359599550235 42-43 0.0024616103521145857 44-45 6.466942450470522E-4 46-47 5.841109310102407E-4 48-49 8.135830824785496E-4 50-51 8.55305291836424E-4 52-53 1.2516662807362302E-4 54-55 2.086110467893717E-4 56-57 0.0042139431451453085 58-59 0.0036924155281718787 60-61 0.005757664891386658 62-63 0.0024616103521145857 64-65 6.466942450470522E-4 66-67 5.006665122944921E-4 68-69 0.003921887679640188 70-71 0.0027953880269775805 72-73 0.021987604331599776 74-75 0.016897494789939106 76-77 0.025471408812982283 78-79 0.020381299271321617 80-81 0.01312163484305148 82-83 0.0045477208200083025 84-85 0.0031917490158773864 86-87 0.0015228606415624132 88-89 0.0020443882585358423 90-91 0.007489136579738443 92-93 0.010931218851763076 94-95 0.014331578914429833 96-97 0.012203746237178244 98-99 0.023072381774904508 100-101 0.020068382701137557 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2396805.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.084466213761715 #Duplication Level Percentage of deduplicated Percentage of total 1 76.46756971259275 39.82772551144006 2 13.264227018122574 13.817203679541409 3 4.441663559897919 6.940250268551991 4 1.945787363714204 4.0538118481814776 5 1.045931283150274 2.7238386289578442 6 0.6163534673510572 1.9261464807588617 7 0.41783844146263865 1.5234024531020158 8 0.28673196170276477 1.1947424937370617 9 0.21034216521411409 0.9860003457681609 >10 1.1655954217131745 11.136295275934568 >50 0.0899977536131562 3.254190224708077 >100 0.04269102409812345 3.9726391768939573 >500 0.002351599902317482 0.8126880308138031 >1k 0.0020272412951009506 2.486930117368423 >5k 5.676275626282662E-4 2.163789273186133 >10k+ 3.243586072161521E-4 3.1803461910561563 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 30546 1.2744466070456295 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 18939 0.7901769230287822 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 13787 0.5752241004170134 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 12029 0.5018764563658704 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 9410 0.3926059900575975 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8611 0.35926994478065594 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 7701 0.3213027342649903 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 7074 0.2951429089976031 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6530 0.2724460271069194 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 6108 0.2548392547578964 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5798 0.24190536985695543 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4768 0.19893149421834483 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4438 0.1851631651302463 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3823 0.1595040063751536 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3733 0.1557490075329449 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3606 0.15045028694449486 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3573 0.149073454035685 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3438 0.14344095577237195 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3155 0.13163357052409355 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2858 0.11924207434480485 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2709 0.11302546515048158 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2652 0.11064729921708273 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2642 0.110230077123504 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 8.344441871574867E-5 0.0 12-13 0.0 0.0 0.0 1.2516662807362302E-4 0.0 14-15 0.0 0.0 0.0 1.2516662807362302E-4 0.0 16-17 0.0 0.0 0.0 1.6688883743149735E-4 0.0 18-19 0.0 0.0 0.0 2.920554655051204E-4 0.0 20-21 0.0 0.0 0.0 3.337776748629947E-4 0.0 22-23 0.0 0.0 0.0 4.3808319825768054E-4 0.0 24-25 0.0 0.0 0.0 5.632498263313036E-4 0.0 26-27 0.0 0.0 0.0 7.30138663762801E-4 0.0 28-29 0.0 0.0 0.0 9.596108152311097E-4 0.0 30-31 0.0 0.0 0.0 0.001376832908809853 0.0 32-33 0.0 0.0 0.0 0.0022947215146830882 0.0 34-35 0.0 0.0 0.0 0.002920554655051204 0.0 36-37 0.0 2.086110467893717E-5 0.0 0.003921887679640188 0.0 38-39 0.0 4.172220935787434E-5 0.0 0.005236137274413229 0.0 40-41 0.0 4.172220935787434E-5 0.0 0.007635164312491003 0.0 42-43 0.0 4.172220935787434E-5 0.0 0.010263663502037087 0.0 44-45 0.0 4.172220935787434E-5 0.0 0.016772328161865482 0.0 46-47 0.0 4.172220935787434E-5 0.0 0.03191749015877387 0.0 48-49 0.0 4.172220935787434E-5 0.0 0.04249407023099501 0.0 50-51 0.0 4.172220935787434E-5 0.0 0.056909093564140596 0.0 52-53 0.0 4.172220935787434E-5 0.0 0.07343108846985884 0.0 54-55 0.0 6.258331403681151E-5 0.0 0.09218522157622336 0.0 56-57 0.0 8.344441871574867E-5 0.0 0.11650926963186409 0.0 58-59 0.0 8.344441871574867E-5 0.0 0.1501373703743108 0.0 60-61 0.0 8.344441871574867E-5 0.0 0.19071221897484358 0.0 62-63 0.0 8.344441871574867E-5 0.0 0.2427398140441129 0.0 64-65 0.0 8.344441871574867E-5 0.0 0.28517130096107113 0.0 66-67 0.0 8.344441871574867E-5 0.0 0.3568917788472571 0.0 68-69 0.0 8.344441871574867E-5 0.0 0.44993230571531684 0.0 70-71 0.0 8.344441871574867E-5 0.0 0.5088649264333144 0.0 72-73 0.0 8.344441871574867E-5 0.0 0.6079551736582659 0.0 74-75 0.0 8.344441871574867E-5 0.0 0.6886250654517159 0.0 76-77 0.0 8.344441871574867E-5 0.0 0.760658459908086 0.0 78-79 0.0 8.344441871574867E-5 0.0 0.8577251799791806 0.0 80-81 0.0 8.344441871574867E-5 0.0 0.9574829825538582 0.0 82-83 0.0 8.344441871574867E-5 0.0 1.0835049159193175 0.0 84-85 0.0 8.344441871574867E-5 0.0 1.2740293849520508 0.0 86-87 0.0 8.344441871574867E-5 0.0 1.5312885278527038 0.0 88-89 0.0 8.344441871574867E-5 0.0 1.8519445678726472 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGCGG 30 0.003954579 31.66276 66-67 GTACACG 285 0.0 26.682032 1 GTACATG 10565 0.0 24.832094 1 TACATGG 10905 0.0 23.620068 2 ACATGGG 10875 0.0 23.20792 3 GTAAGCG 365 0.0 22.774965 94-95 GTATTCG 135 3.782861E-4 21.123276 1 GAGTACT 5840 0.0 20.51631 12-13 CATGGGG 5515 0.0 20.512592 4 TACACCG 300 3.6907295E-9 19.012932 5 GTACTTT 6340 0.0 18.856083 14-15 AGTACTT 6525 0.0 18.216757 12-13 AGAGTAC 12150 0.0 18.00217 10-11 CATGGGA 5420 0.0 17.539606 4 ACCGTCG 170 0.0017757344 16.782425 8 GAGTACA 8630 0.0 16.466608 1 GTGTAGC 1370 0.0 16.30501 1 ACTTTTT 7550 0.0 16.272106 16-17 GTAAGGT 800 0.0 16.042164 4 ATACCGT 210 3.983996E-4 15.844441 6 >>END_MODULE