##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936504_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2396805 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.91426211143585 31.0 31.0 34.0 30.0 34.0 2 32.063694376472014 33.0 31.0 34.0 30.0 34.0 3 32.16446853206665 33.0 31.0 34.0 30.0 34.0 4 35.83060449223028 37.0 35.0 37.0 35.0 37.0 5 35.66464147062444 37.0 35.0 37.0 33.0 37.0 6 35.76368749230747 37.0 35.0 37.0 33.0 37.0 7 35.70836133936636 37.0 35.0 37.0 33.0 37.0 8 35.68558101305697 37.0 35.0 37.0 33.0 37.0 9 37.31816814467593 39.0 37.0 39.0 34.0 39.0 10-11 37.20028746602247 39.0 37.0 39.0 33.0 39.0 12-13 37.11342453808299 39.0 37.0 39.0 33.0 39.0 14-15 38.22515786640966 40.0 38.0 41.0 33.0 41.0 16-17 38.15055521830103 40.0 37.0 41.0 33.0 41.0 18-19 38.307811857869126 40.0 38.0 41.0 33.5 41.0 20-21 38.32151927253156 40.0 38.0 41.0 34.0 41.0 22-23 38.237781963906116 40.0 38.0 41.0 33.5 41.0 24-25 38.086631995510686 40.0 37.0 41.0 33.0 41.0 26-27 37.896251885322336 40.0 37.0 41.0 33.0 41.0 28-29 37.844411414362035 40.0 37.0 41.0 33.0 41.0 30-31 37.87699395653797 40.0 37.0 41.0 33.0 41.0 32-33 37.742293386403986 40.0 37.0 41.0 33.0 41.0 34-35 37.553338298276245 40.0 37.0 41.0 32.0 41.0 36-37 37.36627781567545 39.5 36.0 41.0 31.5 41.0 38-39 37.211740003880166 39.0 36.0 41.0 31.0 41.0 40-41 37.089581964323344 39.0 36.0 41.0 31.0 41.0 42-43 36.82181570882905 39.0 36.0 41.0 31.0 41.0 44-45 36.580945675597306 39.0 35.0 40.5 30.0 41.0 46-47 36.274208581841236 39.0 35.0 40.0 30.0 41.0 48-49 35.91927983294427 39.0 35.0 40.0 29.0 41.0 50-51 35.64864601000082 38.0 35.0 40.0 28.0 41.0 52-53 35.33003185490685 38.0 34.0 40.0 27.0 41.0 54-55 35.16691929464433 38.0 34.0 40.0 27.0 41.0 56-57 34.827720444508415 38.0 34.0 40.0 26.5 41.0 58-59 34.44893869129946 37.5 33.5 40.0 26.0 41.0 60-61 33.97321851381318 37.0 33.0 39.5 25.0 41.0 62-63 33.691998306078304 36.0 33.0 39.0 25.0 40.5 64-65 33.28907003281452 36.0 32.0 39.0 23.5 40.0 66-67 32.82758818510475 35.5 31.5 38.5 22.5 40.0 68-69 32.44487745144056 35.0 31.0 38.0 22.0 40.0 70-71 32.033677750171584 35.0 31.0 37.5 21.5 39.5 72-73 31.55824962815081 34.5 31.0 37.0 20.5 39.0 74-75 31.087764127661615 34.0 30.0 36.0 19.0 39.0 76-77 29.57620728428053 32.5 28.5 35.0 18.0 37.0 78-79 30.37054996130265 34.0 30.0 35.0 18.0 37.0 80-81 30.113546366934315 34.0 30.0 35.0 15.5 37.0 82-83 29.812840844374072 34.0 29.0 35.0 13.0 36.5 84-85 29.424818664847578 34.0 29.0 35.0 8.5 36.0 86-87 29.126003992815434 34.0 29.0 35.0 7.0 36.0 88-89 28.669132657850763 33.5 29.0 35.0 2.0 35.0 90-91 28.185496525583016 33.0 27.5 35.0 2.0 35.0 92-93 27.6081608641504 33.0 26.5 35.0 2.0 35.0 94-95 27.06410200245744 32.5 25.5 34.0 2.0 35.0 96-97 26.342213071150972 32.0 25.0 34.0 2.0 35.0 98-99 25.500378420438878 32.0 23.5 34.0 2.0 35.0 100-101 22.266383998698267 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.39248583670149273 1101 2 0.34919893405301394 1101 3 0.30838054183174535 1101 4 0.28553510810713334 1101 5 0.3468447131817527 1101 6 0.3241966203905804 1101 7 0.3372910692554001 1101 8 0.2473739926193872 1101 9 0.36260201985082574 1101 10-11 0.43301518929195737 1101 12-13 0.5141050680744854 1101 14-15 0.6161993555029852 1101 16-17 0.5534687885369749 1101 18-19 0.4803535224159745 1101 20-21 0.4639400070776247 1101 22-23 0.4722169754855656 1101 24-25 0.5098254374034781 1101 26-27 0.6271002962296635 1101 28-29 0.6017576732603089 1101 30-31 0.6052240765164001 1101 32-33 0.6047373520473869 1101 34-35 0.547510443997993 1101 36-37 0.574765933357618 1101 38-39 0.44184490977650626 1101 40-41 0.493774435853112 1101 42-43 0.631022637061271 1101 44-45 0.7607421194366353 1101 46-47 0.5843744437476275 1101 48-49 0.4975955912914216 1101 50-51 0.48437534683232286 1101 52-53 0.6515324809786378 1101 54-55 0.6502922317865725 1101 56-57 0.5662526954857512 1101 58-59 0.4118351592691454 1101 60-61 0.504235575935347 1101 62-63 0.7419492031178265 1101 64-65 0.7035250529798489 1101 66-67 0.4659193459214279 1101 68-69 0.4731214471860312 1101 70-71 0.4999674348559111 1101 72-73 0.3873743784364656 1101 74-75 0.497994538191346 1101 76-77 0.3924400092324234 1101 78-79 0.4450679840355072 1101 80-81 0.5571537407642353 1101 82-83 0.5381146347224579 1101 84-85 0.4260043520786958 1101 86-87 0.42497090416790684 1101 88-89 0.37194762349551524 1101 90-91 0.5008431428260671 1101 92-93 0.5728706699938684 1101 94-95 0.5837566294305887 1101 96-97 0.8441628770374692 1101 98-99 0.5338390690435268 1101 100-101 1.2231040637663 1102 1 0.29021640995443576 1102 2 0.3352549261404576 1102 3 0.23594039866567584 1102 4 0.2634890080112058 1102 5 0.32394085865586675 1102 6 0.29718123708885 1102 7 0.2980219588295441 1102 8 0.23625423496412168 1102 9 0.37047447189646476 1102 10-11 0.2847727772813258 1102 12-13 0.3305000154739517 1102 14-15 0.4659551930523236 1102 16-17 0.5840416533526707 1102 18-19 0.4799899794373701 1102 20-21 0.39972680270501826 1102 22-23 0.35416350879277303 1102 24-25 0.42853929738250685 1102 26-27 0.43912852320726614 1102 28-29 0.360579250635773 1102 30-31 0.44708676206401776 1102 32-33 0.48585879804281973 1102 34-35 0.3118194503548182 1102 36-37 0.338793549686514 1102 38-39 0.3905882607242077 1102 40-41 0.30908910429479874 1102 42-43 0.3037408065124154 1102 44-45 0.3413458077806837 1102 46-47 0.3242510331969939 1102 48-49 0.40775519442151875 1102 50-51 0.3448508506983643 1102 52-53 0.39158890129641577 1102 54-55 0.25757492914610225 1102 56-57 0.09235728072007987 1102 58-59 0.19444874866093897 1102 60-61 0.3212214423934583 1102 62-63 0.3432809865419557 1102 64-65 0.27771078462950527 1102 66-67 0.10321277491443226 1102 68-69 0.16548387495451067 1102 70-71 0.15411903745297906 1102 72-73 0.2395365858797831 1102 74-75 0.12691063027251204 1102 76-77 0.04285041820534019 1102 78-79 0.2053317661632974 1102 80-81 0.2804302591868719 1102 82-83 0.2775516735419892 1102 84-85 0.41218325015534774 1102 86-87 0.6092529100195279 1102 88-89 0.3241145531163099 1102 90-91 0.3167464662687003 1102 92-93 0.44434917082542214 1102 94-95 0.20880765158795356 1102 96-97 0.37580696458017115 1102 98-99 0.4611634638770781 1102 100-101 1.0941818263856717 1103 1 0.3675158632501869 1103 2 0.41652775139342424 1103 3 0.36290019016080777 1103 4 0.31569575797564653 1103 5 0.3801297951568401 1103 6 0.35237332480192407 1103 7 0.2942132592404221 1103 8 0.33500577000307885 1103 9 0.4123188825516877 1103 10-11 0.452564404632966 1103 12-13 0.4863916602544194 1103 14-15 0.6234030117444362 1103 16-17 0.5982876161751847 1103 18-19 0.48963052489956027 1103 20-21 0.42994598523291927 1103 22-23 0.35858001369187065 1103 24-25 0.49698420526014075 1103 26-27 0.47468062638592556 1103 28-29 0.5039722226114236 1103 30-31 0.5382452381835137 1103 32-33 0.46012333158129337 1103 34-35 0.5479959863453487 1103 36-37 0.5112542997671525 1103 38-39 0.4983581120583125 1103 40-41 0.4864111950328791 1103 42-43 0.6263602190207038 1103 44-45 0.7038740294593424 1103 46-47 0.7229900475266859 1103 48-49 0.6221476996251241 1103 50-51 0.6313718564418735 1103 52-53 0.7945664261224792 1103 54-55 0.7867154275304316 1103 56-57 0.6159200535463398 1103 58-59 0.7585837279166299 1103 60-61 0.7181996443394851 1103 62-63 0.7897265074334996 1103 64-65 0.7672066107258644 1103 66-67 0.6121098907440157 1103 68-69 0.7034508723682364 1103 70-71 0.6465321888053168 1103 72-73 0.6519052436397139 1103 74-75 0.5430475206892602 1103 76-77 0.627869039004139 1103 78-79 0.4633084596142645 1103 80-81 0.6150641436317414 1103 82-83 0.7225250595224004 1103 84-85 1.2040943711494698 1103 86-87 1.0894643797905275 1103 88-89 0.9773329763884036 1103 90-91 0.9802317709626678 1103 92-93 1.0061569008108187 1103 94-95 0.8709858944015672 1103 96-97 0.9907793288707047 1103 98-99 0.7592820520218702 1103 100-101 1.2464520813874458 1104 1 0.22458057878314364 1104 2 0.19207032994444972 1104 3 0.2367018352221848 1104 4 0.24529144150105964 1104 5 0.2759989334412154 1104 6 0.12408139050776867 1104 7 0.13413990592754033 1104 8 0.13693528913755415 1104 9 0.16838307227441618 1104 10-11 0.2964666782512211 1104 12-13 0.2723699924141272 1104 14-15 0.4426983888878553 1104 16-17 0.44401749836024607 1104 18-19 0.29983217219052705 1104 20-21 0.3025328786235022 1104 22-23 0.32290778483250904 1104 24-25 0.2759717697554933 1104 26-27 0.35073926072858086 1104 28-29 0.35676468364479774 1104 30-31 0.4355679723223531 1104 32-33 0.28640260183845356 1104 34-35 0.2681180746480223 1104 36-37 0.29616029493266893 1104 38-39 0.28799091815697153 1104 40-41 0.29068976668706625 1104 42-43 0.46890867066752406 1104 44-45 0.38290739332558843 1104 46-47 0.4333787011738295 1104 48-49 0.573234250217169 1104 50-51 0.7090655190352066 1104 52-53 0.6716160176441193 1104 54-55 0.4788110878310121 1104 56-57 0.433209176706697 1104 58-59 0.47000765172430903 1104 60-61 0.5294129653605566 1104 62-63 0.4782165588439966 1104 64-65 0.5506463757277089 1104 66-67 0.45738549519352745 1104 68-69 0.42969066040912196 1104 70-71 0.40370048709392137 1104 72-73 0.5657240193379955 1104 74-75 0.4618141148325172 1104 76-77 0.39581951251204117 1104 78-79 0.1823511505734885 1104 80-81 0.5798965439461128 1104 82-83 0.36668568618413744 1104 84-85 0.799808832045354 1104 86-87 0.731301408349637 1104 88-89 0.7295630248632747 1104 90-91 0.8844970580123146 1104 92-93 0.8645332620182877 1104 94-95 0.9300364260644471 1104 96-97 0.996664687378523 1104 98-99 1.1876645631651463 1104 100-101 1.6096498250484608 1105 1 0.07433078382588931 1105 2 0.13581666356033395 1105 3 0.1399012233224255 1105 4 0.1588008391218807 1105 5 0.20341431638687624 1105 6 0.1599405161807681 1105 7 0.15537642702874166 1105 8 0.176685590347752 1105 9 0.1428549068883811 1105 10-11 0.15960622615429543 1105 12-13 0.2548538637585622 1105 14-15 0.31366923022942217 1105 16-17 0.2468103385560454 1105 18-19 0.26645828824815254 1105 20-21 0.19036925863331078 1105 22-23 0.368188058003021 1105 24-25 0.2513851867636774 1105 26-27 0.2627208838153692 1105 28-29 0.18851156884971232 1105 30-31 0.2776537172835205 1105 32-33 0.3364843785183993 1105 34-35 0.3853962575502692 1105 36-37 0.3138926286282313 1105 38-39 0.23473741869207743 1105 40-41 0.3335129614522572 1105 42-43 0.3858073699340494 1105 44-45 0.462990066423572 1105 46-47 0.31966240614856645 1105 48-49 0.3542641383744254 1105 50-51 0.3358271865513558 1105 52-53 0.5588943688394039 1105 54-55 0.4359210051682183 1105 56-57 0.27412992167473504 1105 58-59 0.31585998385371994 1105 60-61 0.5092324830945287 1105 62-63 0.47498404449100207 1105 64-65 0.4886370014618464 1105 66-67 0.3334938250808577 1105 68-69 0.4355500917572357 1105 70-71 0.4221685876631014 1105 72-73 0.5164683382457547 1105 74-75 0.43475173512769416 1105 76-77 0.3191958624626494 1105 78-79 0.2675709687620511 1105 80-81 0.2842814878324056 1105 82-83 0.5830755050733494 1105 84-85 0.7599835717406656 1105 86-87 0.8262011675188603 1105 88-89 0.6402738637594396 1105 90-91 0.6685276282715655 1105 92-93 0.6141088372713774 1105 94-95 0.7375367489123228 1105 96-97 0.793876123199798 1105 98-99 0.4475228432514733 1105 100-101 0.9818581354275366 1106 1 0.07592147683348216 1106 2 0.15013224311372397 1106 3 0.08628893489429146 1106 4 0.1539493651706465 1106 5 0.09993971158623083 1106 6 0.06849432523053878 1106 7 0.07334354865190562 1106 8 0.05750065243688596 1106 9 0.11983810343669177 1106 10-11 0.07446091864113669 1106 12-13 0.11540554837757355 1106 14-15 0.1978151807921904 1106 16-17 0.12053262265772702 1106 18-19 0.23163333844942002 1106 20-21 0.1055477772258584 1106 22-23 0.054096296533664656 1106 24-25 0.042013363382700675 1106 26-27 -0.04738395365351522 1106 28-29 -0.31531179831058154 1106 30-31 -0.05482964101346255 1106 32-33 0.03363283487058055 1106 34-35 0.04224110309344553 1106 36-37 -0.22973504893695917 1106 38-39 -0.06368830973584494 1106 40-41 0.01059011688484901 1106 42-43 0.07996207859574866 1106 44-45 -0.0846035768535387 1106 46-47 -0.08581883560784576 1106 48-49 -0.2479645578129137 1106 50-51 -0.13025564520766864 1106 52-53 -0.294946964916484 1106 54-55 -0.24495025801002157 1106 56-57 -0.2887032740188076 1106 58-59 -0.33025446451097196 1106 60-61 -0.3858864054685327 1106 62-63 -0.21682744531948828 1106 64-65 -0.177413053177645 1106 66-67 -0.22863393927630682 1106 68-69 -0.27818689109083294 1106 70-71 -0.12998631891060342 1106 72-73 -0.09104069600946119 1106 74-75 -0.24456129984289632 1106 76-77 -0.23886615829115243 1106 78-79 -0.28115647683187817 1106 80-81 -0.0478231418617554 1106 82-83 -0.12857395004402505 1106 84-85 0.038019433262761027 1106 86-87 0.11949786850342647 1106 88-89 0.02628732111762133 1106 90-91 0.16618687510136 1106 92-93 0.14357578005048666 1106 94-95 0.23772553788816353 1106 96-97 0.507875290128375 1106 98-99 0.5225690501135638 1106 100-101 1.0426438296120821 1107 1 0.08520611115551802 1107 2 0.11804282438296809 1107 3 0.0707139588400878 1107 4 0.1152414088351108 1107 5 0.0928481159653387 1107 6 0.03470899034567765 1107 7 0.04853475876534219 1107 8 0.1129361492032146 1107 9 0.1029261210417971 1107 10-11 0.13553408589753957 1107 12-13 0.007629459338808431 1107 14-15 0.07706371831781667 1107 16-17 0.14314552409605596 1107 18-19 0.01924873341644684 1107 20-21 -0.021473207995036603 1107 22-23 -0.03620965547975885 1107 24-25 -0.019926047827887317 1107 26-27 0.039486707408649124 1107 28-29 0.0346511549773183 1107 30-31 -0.002127051701599214 1107 32-33 -0.004175709621087265 1107 34-35 0.13284612023150544 1107 36-37 0.16518709755587224 1107 38-39 0.059670196363398986 1107 40-41 0.03606740994175084 1107 42-43 0.10796598908202526 1107 44-45 0.22839419876054023 1107 46-47 0.16333296883617976 1107 48-49 0.014521371560853424 1107 50-51 0.08859479848212004 1107 52-53 0.17129010237712095 1107 54-55 0.10836352289192774 1107 56-57 0.056166231312595016 1107 58-59 0.03879189929090643 1107 60-61 0.039256913061947785 1107 62-63 0.14226688922498454 1107 64-65 0.11129316164578995 1107 66-67 0.2266646113106603 1107 68-69 0.2133074846166494 1107 70-71 0.13457485961813376 1107 72-73 0.18762261428841853 1107 74-75 0.26908621810655475 1107 76-77 0.09380753775165829 1107 78-79 0.11842809337085214 1107 80-81 0.33973288337433516 1107 82-83 0.23711778648102566 1107 84-85 0.4041911146927042 1107 86-87 0.30452268505095503 1107 88-89 0.2796314993218054 1107 90-91 0.46425094691498714 1107 92-93 0.4836424324684181 1107 94-95 0.47694771525325663 1107 96-97 0.769228840834085 1107 98-99 0.740942240326266 1107 100-101 1.3087903382761041 1108 1 0.07307234399963036 1108 2 0.07480444825257138 1108 3 0.07366535666940877 1108 4 0.1305292844310273 1108 5 0.16095934931662015 1108 6 0.08633855582089467 1108 7 0.04459742895876673 1108 8 0.07901060126815906 1108 9 0.12925478063934293 1108 10-11 0.12261608304180527 1108 12-13 0.12075937983068741 1108 14-15 0.0553587800751103 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-1.2751840047084677 2316 86-87 -1.5341513106766556 2316 88-89 -1.5357511922845077 2316 90-91 -1.6040479120440096 2316 92-93 -1.6002284232254986 2316 94-95 -1.5986560639023395 2316 96-97 -1.9701382895433106 2316 98-99 -2.4840983126531206 2316 100-101 -3.3749915961096946 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 10.0 4 21.0 5 82.0 6 144.0 7 345.0 8 771.0 9 1430.0 10 2452.0 11 3935.0 12 5539.0 13 7808.0 14 9919.0 15 12916.0 16 16038.0 17 20259.0 18 18578.0 19 13807.0 20 13104.0 21 14003.0 22 15322.0 23 17380.0 24 20495.0 25 24732.0 26 30851.0 27 38092.0 28 47683.0 29 58950.0 30 73690.0 31 90952.0 32 113513.0 33 144937.0 34 190216.0 35 257611.0 36 367390.0 37 469524.0 38 277865.0 39 16438.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.99602717545529 19.00070108668436 14.741933329994824 22.261338407865523 2 15.55768180359616 21.4093797582392 37.97006552469621 25.062872913468425 3 21.754151859089028 23.537779090761532 29.46489931264466 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25.956086485241585 28.402596459782657 23.169310606383007 24-25 23.63590279559664 26.076067932101278 28.159424734177374 22.128604538124712 26-27 22.919765893020493 25.372465205740646 29.617036279071705 22.09073262216715 28-29 21.805021842260082 27.850425371234977 28.443462551059152 21.901090235445793 30-31 22.424849682676175 25.883994370740872 28.17345728384492 23.517698662738034 32-33 22.446743835061422 26.295629141062438 28.600645837792822 22.656981186083318 34-35 23.919749577291725 25.80876792403038 28.118690016365928 22.152792482311966 36-37 23.141121536464944 25.332837945330276 29.198387249196784 22.327653269007993 38-39 22.431749756338537 26.50803081483064 28.974039206130925 22.0861802226999 40-41 22.710304061982708 26.72724750422178 28.21058099401389 22.351867439781625 42-43 22.243476417717893 26.862985057065046 27.21729196282646 23.676246562390602 44-45 23.05896445560215 25.573370428025633 27.6311161494174 23.73654896695482 46-47 24.04452111162592 26.830011154476885 27.355108101629867 21.770359632267333 48-49 23.595350681737155 26.528367312581473 27.40331148250268 22.472970523178688 50-51 21.833748400472132 26.95814128916001 28.678809796891773 22.529300513476088 52-53 23.31839884045418 27.663962853812823 26.488625148400963 22.529013157332034 54-55 22.430509182448823 26.37326806225119 27.383730669585592 23.8124920857144 56-57 23.461692215760767 26.22242149996328 27.10629802973754 23.209588254538414 58-59 24.805062612837073 26.005215023742213 26.562441312202807 22.62728105121791 60-61 23.104624593094357 26.682347874513333 28.457636430368403 21.755391102023903 62-63 22.46352696943933 26.800002086408615 28.109452995821965 22.627017948330092 64-65 22.210630465889842 28.05901001486017 27.153399414690494 22.576960104559497 66-67 22.90237895262998 26.686435292797217 26.469180678483617 23.942005076089185 68-69 22.493612762000883 26.696266206436448 27.212147674486282 23.597973357076384 70-71 23.79575943100825 26.484489116952137 26.81207159042407 22.907679861615545 72-73 23.282499678217395 25.697840604020893 28.355420727889992 22.664238989871723 74-75 22.361947947207042 28.307289135036108 26.778808382486517 22.551954535270337 76-77 22.871050999980806 26.46066792225793 26.806636998664025 23.861644079097243 78-79 23.023151982741894 26.743408768002404 27.144505342043058 23.088933907212645 80-81 23.940640082091015 26.499036352775214 26.708812453197332 22.851511111936436 82-83 23.301482976803385 26.20730377812205 27.829888007344465 22.6613252377301 84-85 22.711388069446773 27.127673892897587 27.38870130463625 22.772236733019394 86-87 22.84778761172727 27.325307378438662 26.735680726587947 23.09122428324612 88-89 22.404065266485198 27.483060520070783 25.960735175620254 24.152139037823765 90-91 23.041249883383816 26.112528825329097 26.627706252025785 24.218515039261305 92-93 23.903724858529998 26.789612748430674 26.876483405680336 22.430178987358996 94-95 23.10672994817338 26.351548746987767 27.256657370873754 23.28506393396509 96-97 21.654991163017083 26.536829389041806 28.521685880947434 23.286493566993684 98-99 22.49338613985379 27.348559561267567 26.34728114254108 23.810773156337564 100-101 21.857044990872076 26.26437287848319 27.063781076790193 24.814801053854538 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 179.0 1 141.5 2 132.5 3 169.0 4 161.5 5 159.0 6 261.5 7 507.0 8 822.0 9 1069.0 10 1075.0 11 997.0 12 1085.5 13 1230.0 14 1416.5 15 1635.5 16 1839.0 17 2066.0 18 2258.5 19 2479.5 20 2762.5 21 3198.0 22 3753.5 23 4520.5 24 5585.5 25 7092.5 26 9309.5 27 12045.0 28 14885.5 29 18536.0 30 23242.5 31 28311.5 32 34324.0 33 39209.0 34 42835.5 35 46567.5 36 52671.5 37 58268.0 38 60276.0 39 62112.0 40 64344.5 41 67331.0 42 69241.5 43 71617.0 44 75325.0 45 79045.5 46 82599.0 47 107892.0 48 158777.5 49 153748.0 50 105101.5 51 84042.0 52 77866.0 53 76321.0 54 75499.0 55 71212.0 56 65634.5 57 60576.5 58 55936.5 59 50012.0 60 41974.0 61 34525.5 62 28584.0 63 22370.5 64 16563.5 65 12718.0 66 9662.5 67 7428.5 68 5404.0 69 3764.0 70 2904.0 71 2242.5 72 1760.5 73 1450.0 74 1182.5 75 981.5 76 708.0 77 417.5 78 286.5 79 217.0 80 153.0 81 108.5 82 68.0 83 42.5 84 21.5 85 14.5 86 10.0 87 4.0 88 4.0 89 6.0 90 3.0 91 0.5 92 0.0 93 0.0 94 0.5 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021904159912884028 2 0.0126835516447938 3 0.009512663733595349 4 0.0 5 0.0036715544234929417 6 0.0 7 4.172220935787434E-5 8 0.0 9 0.0 10-11 0.0 12-13 0.0011264996526626071 14-15 2.9205546550512034E-4 16-17 2.086110467893717E-5 18-19 0.0015645828509202877 20-21 0.0 22-23 1.2516662807362302E-4 24-25 0.0 26-27 0.0015645828509202877 28-29 0.0031291657018405754 30-31 0.0017523327930307221 32-33 0.009512663733595349 34-35 0.002399027038077774 36-37 0.0012725273854151673 38-39 0.0015437217462413505 40-41 3.129165701840575E-4 42-43 0.006425220241112648 44-45 0.0023364437240409627 46-47 3.9636098889980623E-4 48-49 9.80471919910047E-4 50-51 1.6688883743149735E-4 52-53 1.877499421104345E-4 54-55 0.0013142495947730416 56-57 0.015562384090487128 58-59 0.027161158291976194 60-61 0.021632965552057844 62-63 0.014289856705071961 64-65 0.01944254956076944 66-67 0.0032543323299141984 68-69 0.0013976940134887903 70-71 1.6688883743149735E-4 72-73 2.086110467893717E-5 74-75 0.0019192216304622196 76-77 0.0024616103521145857 78-79 0.010430552339468584 80-81 0.018378633222143646 82-83 0.018462077640859394 84-85 0.029059518817759474 86-87 0.012036857399746746 88-89 0.04157618162512178 90-91 0.04695834663228756 92-93 0.029789657481522278 94-95 0.030832712715469134 96-97 0.049691151345228335 98-99 0.05288290036110572 100-101 0.05874487077588707 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2396805.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.540155286635255 #Duplication Level Percentage of deduplicated Percentage of total 1 74.86089774328423 37.086204990992236 2 13.99095491618851 13.862281583125835 3 4.732567726051083 7.033564201592668 4 2.13892430621169 4.238505691043428 5 1.1259397687089308 2.7889615492619306 6 0.7187835765916357 2.1365190001099634 7 0.4422352520627875 1.5335882142290345 8 0.3302628215324838 1.3089017171297246 9 0.22865286357222828 1.0194748531291813 >10 1.2744810821704584 11.642426013446658 >50 0.10026398004839221 3.4358854772498546 >100 0.050021205368614506 4.444470522094999 >500 0.0027108769392886188 0.8862360422475803 >1k 0.0021178726088191438 2.235168128714415 >5k 8.471490435276577E-4 3.093159777514383 >10k+ 3.3885961741106303E-4 3.254652238118095 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 35433 1.4783430441775613 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 16877 0.7041457273328452 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 13061 0.5449337764231967 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 12180 0.5081765099789094 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 9891 0.4126743727587351 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 9398 0.39210532354530303 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 9124 0.38067343818124544 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8165 0.340661839407044 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 7416 0.3094119045979961 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 6690 0.27912158060417935 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6624 0.2763679147865596 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5894 0.24591070195531134 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5326 0.2222124870400387 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5175 0.21591243342699967 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4299 0.17936377802950176 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3724 0.155373507648724 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3693 0.15408011915862993 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3525 0.14707078798650705 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3236 0.13501306948208136 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3109 0.12971434889363131 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2943 0.12278846214022418 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2889 0.12053546283489897 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2837 0.1183659079482895 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2707 0.11294202073176583 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 4.172220935787434E-5 0.0 7 0.0 0.0 0.0 4.172220935787434E-5 0.0 8 0.0 0.0 0.0 4.172220935787434E-5 0.0 9 0.0 0.0 0.0 8.344441871574867E-5 0.0 10-11 0.0 0.0 0.0 1.2516662807362302E-4 0.0 12-13 0.0 2.086110467893717E-5 0.0 1.6688883743149735E-4 0.0 14-15 0.0 4.172220935787434E-5 0.0 1.6688883743149735E-4 0.0 16-17 0.0 4.172220935787434E-5 0.0 1.6688883743149735E-4 0.0 18-19 0.0 4.172220935787434E-5 0.0 2.920554655051204E-4 0.0 20-21 0.0 4.172220935787434E-5 0.0 3.9636098889980623E-4 0.0 22-23 0.0 4.172220935787434E-5 0.0 5.006665122944921E-4 0.0 24-25 0.0 4.172220935787434E-5 0.0 5.841109310102407E-4 0.0 26-27 0.0 4.172220935787434E-5 0.0 6.884164544049265E-4 0.0 28-29 0.0 4.172220935787434E-5 0.0 9.80471919910047E-4 0.0 30-31 0.0 4.172220935787434E-5 0.0 0.0013976940134887903 0.0 32-33 0.0 4.172220935787434E-5 0.0 0.0022738604100041512 0.0 34-35 0.0 8.344441871574867E-5 0.0 0.002941415759730141 0.0 36-37 0.0 8.344441871574867E-5 0.0 0.003984470993676999 0.0 38-39 0.0 1.0430552339468584E-4 0.0 0.005340442797807914 0.0 40-41 0.0 1.2516662807362302E-4 0.0 0.007718608731206752 0.0 42-43 0.0 1.2516662807362302E-4 0.0 0.010409691234789648 0.0 44-45 0.0 1.2516662807362302E-4 0.0 0.01685577258058123 0.0 46-47 0.0 1.2516662807362302E-4 0.0 0.03202179568216855 0.0 48-49 0.0 1.6688883743149735E-4 0.0 0.042577514649710754 0.0 50-51 0.0 1.6688883743149735E-4 0.0 0.05703426019221422 0.0 52-53 0.0 1.6688883743149735E-4 0.0 0.07351453288857458 0.0 54-55 0.0 1.6688883743149735E-4 0.0 0.09243555483237059 0.0 56-57 0.0 1.6688883743149735E-4 0.0 0.11671788067865346 0.0 58-59 0.0 1.6688883743149735E-4 0.0 0.1500330648509161 0.0 60-61 0.0 2.0861104678937168E-4 0.0 0.1904827468233753 0.0 62-63 0.0 2.0861104678937168E-4 0.0 0.24265636962539716 0.0 64-65 0.0 2.0861104678937168E-4 0.0 0.2847540788674924 0.0 66-67 0.0 2.0861104678937168E-4 0.0 0.35626594570688896 0.0 68-69 0.0 2.0861104678937168E-4 0.0 0.4491604448421962 2.086110467893717E-5 70-71 0.0 2.0861104678937168E-4 0.0 0.5078635934087254 4.172220935787434E-5 72-73 0.0 2.0861104678937168E-4 0.0 0.6063697297026667 4.172220935787434E-5 74-75 2.086110467893717E-5 2.0861104678937168E-4 0.0 0.6868727326586852 4.172220935787434E-5 76-77 8.344441871574867E-5 2.0861104678937168E-4 0.0 0.7585306272308343 4.172220935787434E-5 78-79 8.344441871574867E-5 2.0861104678937168E-4 0.0 0.8553261529411029 4.172220935787434E-5 80-81 8.344441871574867E-5 2.0861104678937168E-4 0.0 0.9548336222596332 4.172220935787434E-5 82-83 8.344441871574867E-5 2.0861104678937168E-4 0.0 1.0802297224847246 4.172220935787434E-5 84-85 8.344441871574867E-5 2.0861104678937168E-4 0.0 1.2690644420384638 4.172220935787434E-5 86-87 8.344441871574867E-5 2.0861104678937168E-4 0.0 1.524529529936728 4.172220935787434E-5 88-89 8.344441871574867E-5 2.0861104678937168E-4 0.0 1.8424944874530886 4.172220935787434E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10555 0.0 23.812534 1 TAAGGTG 885 0.0 23.075554 5 TACATGG 10800 0.0 22.961037 2 ACATGGG 10800 0.0 21.77067 3 GAGTACT 7445 0.0 20.445576 12-13 CATGGGG 5365 0.0 19.740679 4 GTACTTT 7805 0.0 19.380434 14-15 AGTACTT 7885 0.0 19.154087 12-13 GTAAGGT 860 0.0 18.776186 4 AGAGTAC 13725 0.0 18.097403 10-11 ACCGTCC 215 2.399779E-5 17.671335 8 GGTAAGG 1045 0.0 16.818058 3 GTAAGCG 285 0.0 16.673847 94-95 ACTTTTT 9250 0.0 16.583593 16-17 CATGGGA 5225 0.0 16.452019 4 GTGTACG 260 6.612285E-6 16.446646 1 TACTTTT 9075 0.0 16.380405 14-15 GAGTACA 8590 0.0 15.819075 1 AAGGTGT 1575 0.0 15.679479 6 GTACCTG 1705 0.0 15.6052885 1 >>END_MODULE