FastQCFastQC Report
Thu 26 May 2016
SRR936503_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936503_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2057834
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT67370.3273830639400457No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54210.2634323273888953No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43450.2111443391449456No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT42890.2084230312065988No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT29160.14170239193248824No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC26550.1290191531484075No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23640.11487807082592669No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT21190.10297234859565933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG111000.024.5664331
GTATAGG5250.024.4319211
TACATGG109700.024.2518632
ACATGGG112000.023.2007143
TACCGTA1302.9379415E-421.9282177
GAGTACT63450.021.453812-13
GTACTTT67450.020.14679114-15
GTATCAA183450.020.0695271
CATGGGG57550.019.8110964
AGTACTT67150.019.45798912-13
ATGGGAG24800.018.9642945
CATGGGA55500.018.8309544
AGAGTAC138000.017.95526110-11
TACTTTT72300.017.64524814-15
TCAACGC207500.017.60564
ATCAACG208850.017.559613
CAACGCA208500.017.521595
TATCAAC209100.017.5171762
ACTTTTT80450.017.45232416-17
GTACACG4405.456968E-1217.2750971
Produced by FastQC (version 0.11.5)