##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936503_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2057834 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.239938206871887 31.0 31.0 34.0 28.0 34.0 2 31.30426944058656 31.0 31.0 34.0 28.0 34.0 3 31.316371485746664 31.0 31.0 34.0 28.0 34.0 4 34.942312645237664 37.0 35.0 37.0 32.0 37.0 5 34.95355796434504 37.0 35.0 37.0 32.0 37.0 6 34.93665086688236 37.0 35.0 37.0 32.0 37.0 7 34.84074468591733 37.0 35.0 37.0 32.0 37.0 8 34.78701780610098 37.0 35.0 37.0 32.0 37.0 9 36.262803024928154 39.0 35.0 39.0 32.0 39.0 10-11 36.110486560140416 39.0 35.0 39.0 31.0 39.0 12-13 35.99814999654977 39.0 35.0 39.0 30.0 39.0 14-15 37.06627623996882 39.0 36.0 41.0 31.0 41.0 16-17 36.94700908819662 39.0 36.0 41.0 31.0 41.0 18-19 36.89326082667504 39.0 36.0 41.0 30.5 41.0 20-21 36.735591646362145 39.0 36.0 41.0 30.0 41.0 22-23 36.84361396497482 39.0 36.0 41.0 30.0 41.0 24-25 36.87866781285565 39.0 36.0 41.0 30.0 41.0 26-27 36.7692289562715 39.0 36.0 41.0 30.0 41.0 28-29 36.62778776130631 39.0 36.0 41.0 30.0 41.0 30-31 36.39942507510324 39.0 35.5 41.0 30.0 41.0 32-33 36.2144640918558 39.0 35.0 40.5 29.5 41.0 34-35 36.02050262557621 38.5 35.0 40.0 29.0 41.0 36-37 35.79307490302911 38.0 35.0 40.0 27.5 41.0 38-39 35.56441238700498 38.0 35.0 40.0 27.0 41.0 40-41 35.33520196478433 38.0 34.0 40.0 26.5 41.0 42-43 35.07071391569971 38.0 34.0 40.0 25.5 41.0 44-45 34.77251590750274 38.0 33.5 40.0 24.5 41.0 46-47 34.57614438288025 38.0 33.0 40.0 24.0 41.0 48-49 34.39079998678222 38.0 33.0 40.0 23.5 41.0 50-51 33.39133890294358 37.0 32.0 39.5 22.5 40.5 52-53 33.47151908268597 37.5 32.5 39.5 22.5 40.0 54-55 33.87907333633325 38.0 33.0 40.0 22.5 41.0 56-57 33.73337742500124 37.5 33.0 40.0 22.0 41.0 58-59 33.45995984126999 37.0 33.0 40.0 21.5 41.0 60-61 33.11152332987014 36.5 32.0 40.0 20.0 41.0 62-63 32.71360930959446 36.0 32.0 39.0 18.5 41.0 64-65 32.32587492479957 35.5 31.0 39.0 18.0 41.0 66-67 31.840215245738968 35.0 31.0 38.5 14.0 40.0 68-69 31.4186020349552 35.0 31.0 38.0 12.0 40.0 70-71 31.01841232091607 35.0 30.0 37.0 9.0 39.5 72-73 30.617929337351796 35.0 30.0 37.0 7.5 39.0 74-75 30.191775186919838 34.0 29.5 36.0 6.5 39.0 76-77 29.740319189983254 34.0 29.0 35.5 2.0 38.0 78-79 29.257437917732915 34.0 29.0 35.0 2.0 37.0 80-81 28.806368249334007 34.0 28.5 35.0 2.0 37.0 82-83 28.40803194038003 34.0 27.5 35.0 2.0 36.0 84-85 28.039721376942943 33.5 27.0 35.0 2.0 36.0 86-87 27.68387221709817 33.0 26.0 35.0 2.0 35.5 88-89 27.261228310932758 33.0 25.5 35.0 2.0 35.0 90-91 26.833656407659703 33.0 25.0 35.0 2.0 35.0 92-93 26.362919701006007 33.0 24.5 35.0 2.0 35.0 94-95 25.833302394653796 32.0 23.5 35.0 2.0 35.0 96-97 25.192236108451898 32.0 19.5 35.0 2.0 35.0 98-99 24.372772536560284 32.0 8.5 34.0 2.0 35.0 100-101 21.436581376340364 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5074753019301674 1101 2 0.4943349337358782 1101 3 0.4018197022565637 1101 4 0.5293666005225077 1101 5 0.3559284267524703 1101 6 0.5571285181490708 1101 7 0.6398421260431917 1101 8 0.7653466676071687 1101 9 0.6960746864243603 1101 10-11 0.8089437251037594 1101 12-13 0.9410586019627516 1101 14-15 0.9602332730732499 1101 16-17 0.9504918600988077 1101 18-19 0.9027615869270065 1101 20-21 1.0169132553463456 1101 22-23 0.8495325211995137 1101 24-25 0.9451097327708524 1101 26-27 0.8691278997447114 1101 28-29 0.8715678963868001 1101 30-31 0.894796037183859 1101 32-33 0.8680911862375922 1101 34-35 0.8519080887464909 1101 36-37 0.7126790955516853 1101 38-39 0.686827136261364 1101 40-41 0.7663231857838397 1101 42-43 0.8487537679818189 1101 44-45 0.7850253812544494 1101 46-47 0.9092320358114705 1101 48-49 1.0759685936780627 1101 50-51 0.9250442905842746 1101 52-53 1.1771356501626755 1101 54-55 1.0151110029789407 1101 56-57 0.978045330753595 1101 58-59 0.9466039662642558 1101 60-61 1.0185067819608022 1101 62-63 1.0328431497402661 1101 64-65 0.9549156035847659 1101 66-67 1.0611693847851207 1101 68-69 0.8195820452155509 1101 70-71 0.8517170961469631 1101 72-73 0.8793954618197439 1101 74-75 0.8429752169319826 1101 76-77 0.7438774915486199 1101 78-79 0.7377033345828146 1101 80-81 0.9262421963497616 1101 82-83 0.8233747630274273 1101 84-85 0.862952175347818 1101 86-87 1.0593608944195871 1101 88-89 1.0008406815050606 1101 90-91 0.8154780181045815 1101 92-93 0.5784642024912863 1101 94-95 0.6624650729078674 1101 96-97 0.6206986935997314 1101 98-99 0.5953978080083182 1101 100-101 0.82030829136691 1102 1 0.41377391464630264 1102 2 0.5555843945132892 1102 3 0.6334175883743107 1102 4 0.4980710681519085 1102 5 0.5569311537985797 1102 6 0.6096609253295 1102 7 0.57760902987701 1102 8 0.6780736696177527 1102 9 0.6862764639717938 1102 10-11 0.7991850607727073 1102 12-13 0.8441928243551331 1102 14-15 0.9074034666382715 1102 16-17 0.7407694854805413 1102 18-19 0.7167631905224638 1102 20-21 0.7354063788478342 1102 22-23 0.6788141949983171 1102 24-25 0.8040524751784801 1102 26-27 0.5412893840380661 1102 28-29 0.476449446401098 1102 30-31 0.629142499403784 1102 32-33 0.5840419546638316 1102 34-35 0.7267599403389511 1102 36-37 0.5000225459070222 1102 38-39 0.5182651816284363 1102 40-41 0.41564004944995503 1102 42-43 0.4558472006332721 1102 44-45 0.5919416713407699 1102 46-47 0.8146759337053453 1102 48-49 0.7578774585104284 1102 50-51 0.8913789024879719 1102 52-53 1.0464083799350519 1102 54-55 0.9561984544424291 1102 56-57 0.8860843711176614 1102 58-59 0.8915132510611983 1102 60-61 0.7122061718837784 1102 62-63 0.624116576254373 1102 64-65 0.7979924264943392 1102 66-67 0.7974346104467038 1102 68-69 0.7512917576033651 1102 70-71 0.9285239230746036 1102 72-73 0.6037103735195295 1102 74-75 0.6756569436997566 1102 76-77 0.7715828325694609 1102 78-79 0.6384110228917912 1102 80-81 0.8726799810223262 1102 82-83 1.0288436668420466 1102 84-85 0.7097735311304518 1102 86-87 0.9124853504954125 1102 88-89 0.848031938148555 1102 90-91 0.798961010835999 1102 92-93 0.9982102419451273 1102 94-95 0.5612812345145954 1102 96-97 0.6794540472402559 1102 98-99 0.8663832617736951 1102 100-101 1.1647236054700691 1103 1 0.3550619410661042 1103 2 0.22802919137379973 1103 3 0.2712625125444461 1103 4 0.5248688889825459 1103 5 0.5008264617913483 1103 6 0.3935226587685605 1103 7 0.5026588480148462 1103 8 0.554338732655566 1103 9 0.3622407244213832 1103 10-11 0.5038534876191534 1103 12-13 0.12326334191396882 1103 14-15 0.3864321992721713 1103 16-17 0.4668873849450321 1103 18-19 0.7413624156686822 1103 20-21 0.7298673105539208 1103 22-23 0.5018712876227909 1103 24-25 0.5631733790035085 1103 26-27 0.3762928111349737 1103 28-29 0.41727954914070864 1103 30-31 0.2948898569959937 1103 32-33 0.36897824487112985 1103 34-35 0.5876422864525281 1103 36-37 0.5724105299310054 1103 38-39 0.416753349286175 1103 40-41 0.4880263229799411 1103 42-43 0.492300515753918 1103 44-45 0.42432498815968955 1103 46-47 0.551764452991776 1103 48-49 0.5250595540366305 1103 50-51 0.4330074393924903 1103 52-53 0.5694179452618755 1103 54-55 0.7094346480068054 1103 56-57 0.5293174735750341 1103 58-59 0.45332195185548585 1103 60-61 0.5929930479963659 1103 62-63 0.7449086082845966 1103 64-65 0.6786306118068097 1103 66-67 0.6929602024914381 1103 68-69 0.5175895277300739 1103 70-71 0.4313820363752825 1103 72-73 0.3422867782321859 1103 74-75 0.3684062817996683 1103 76-77 0.3657522472261938 1103 78-79 0.2988938464775508 1103 80-81 0.472422307318606 1103 82-83 0.39892162871514003 1103 84-85 0.4781752088807103 1103 86-87 0.40648163872342735 1103 88-89 0.23043339842172017 1103 90-91 0.16966562440486044 1103 92-93 0.06140578684131981 1103 94-95 0.03729008998640637 1103 96-97 0.18349093526521187 1103 98-99 0.30526401224071975 1103 100-101 0.4544034620817996 1104 1 0.46049984650401043 1104 2 0.46549522883983485 1104 3 0.35870579998773167 1104 4 0.4785160426296997 1104 5 0.506190267155155 1104 6 0.6094762747221765 1104 7 0.6521719975279936 1104 8 0.5488761271929619 1104 9 0.7105656727463341 1104 10-11 0.7246704084360758 1104 12-13 0.7196493382999662 1104 14-15 0.4328162456562197 1104 16-17 0.4842398022974521 1104 18-19 0.5219988963051634 1104 20-21 0.41364719433379804 1104 22-23 0.5642687500202541 1104 24-25 0.5566408724710001 1104 26-27 0.3514900863322481 1104 28-29 0.40149493335608355 1104 30-31 0.5247677868739231 1104 32-33 0.4994957753886595 1104 34-35 0.40506880387903976 1104 36-37 0.2961394536599258 1104 38-39 0.4240450565778744 1104 40-41 0.23932957428318957 1104 42-43 0.5427561662095712 1104 44-45 0.41191127574597886 1104 46-47 0.35825275948008084 1104 48-49 0.6717114006884728 1104 50-51 0.6063171127021647 1104 52-53 0.5945898704338006 1104 54-55 0.5514129899851525 1104 56-57 0.41327285753041565 1104 58-59 0.5028065013400322 1104 60-61 0.44387379887711376 1104 62-63 0.6356044989804914 1104 64-65 0.5280852612614666 1104 66-67 0.5604783700096085 1104 68-69 0.508432318572865 1104 70-71 0.4275119325051797 1104 72-73 0.2309390668844742 1104 74-75 0.3602539736473602 1104 76-77 0.08209196356591164 1104 78-79 0.019075066658768236 1104 80-81 0.22562096051726144 1104 82-83 0.22784535763886637 1104 84-85 0.24499527570077717 1104 86-87 0.15369118593297415 1104 88-89 0.12566369365201524 1104 90-91 0.09850615324539191 1104 92-93 -0.02449699906146563 1104 94-95 0.1700696227659364 1104 96-97 0.16631921809349848 1104 98-99 0.2763623833390909 1104 100-101 0.18636659404493017 1105 1 0.41057009699738956 1105 2 0.2511908579769333 1105 3 0.3780828602674724 1105 4 0.31960949444247433 1105 5 0.22884633959966294 1105 6 0.4667052913306051 1105 7 0.5794751180610191 1105 8 0.5670238886411454 1105 9 0.5243920281805856 1105 10-11 0.5338275314154828 1105 12-13 0.39256663674618153 1105 14-15 0.4718040822463152 1105 16-17 0.44339037851432295 1105 18-19 0.4806108062569834 1105 20-21 0.4852976094256789 1105 22-23 0.19371893729836387 1105 24-25 0.28442546837971605 1105 26-27 0.10093734283169908 1105 28-29 0.2878495806416268 1105 30-31 0.18419502316998404 1105 32-33 0.021944725905314044 1105 34-35 0.18420952942314983 1105 36-37 0.3106695351857809 1105 38-39 0.2693057093138478 1105 40-41 0.2163277106607353 1105 42-43 0.06823494486183534 1105 44-45 0.3554663102742168 1105 46-47 0.3480781407017943 1105 48-49 0.18255629940362184 1105 50-51 0.2431817629659676 1105 52-53 0.3695481305824657 1105 54-55 0.20002653493156686 1105 56-57 0.1557052410065225 1105 58-59 0.3012170966476617 1105 60-61 0.3085576445200573 1105 62-63 0.2733911233679578 1105 64-65 0.2926141555505808 1105 66-67 0.5443350033725842 1105 68-69 0.582240112408865 1105 70-71 0.4250112727407682 1105 72-73 0.2943612530146993 1105 74-75 0.6739019043615819 1105 76-77 0.46096600181935443 1105 78-79 0.6396172471445318 1105 80-81 0.6522829732159465 1105 82-83 0.5201186174720682 1105 84-85 0.4108176474469829 1105 86-87 0.43694789161563463 1105 88-89 0.5746752681925038 1105 90-91 0.4430710852574329 1105 92-93 0.21447611579317183 1105 94-95 0.3661834916583686 1105 96-97 0.369685746565807 1105 98-99 0.551428512424966 1105 100-101 0.3586553354450963 1106 1 0.46533387867074794 1106 2 0.5404295276195654 1106 3 0.6035833262036192 1106 4 0.5035341878174933 1106 5 0.45688386440860285 1106 6 0.5783737681248553 1106 7 0.6529875676178349 1106 8 0.7605653002393495 1106 9 0.7835154478638771 1106 10-11 0.8274902680891074 1106 12-13 0.7749547600050093 1106 14-15 0.8316770542719851 1106 16-17 0.8721930976494292 1106 18-19 0.6082393131299355 1106 20-21 0.35622022503124384 1106 22-23 0.6308985369610696 1106 24-25 0.6832353482643541 1106 26-27 0.6057971033631162 1106 28-29 0.7270857909921844 1106 30-31 0.7871271418345955 1106 32-33 0.6462048888744008 1106 34-35 0.7012024621710395 1106 36-37 0.5241715360746682 1106 38-39 0.5215148625773693 1106 40-41 0.46693743784769737 1106 42-43 0.44534722195802345 1106 44-45 0.4500532311829417 1106 46-47 0.8039007251563248 1106 48-49 0.6922245298255447 1106 50-51 0.2593348807057936 1106 52-53 0.2799442671454031 1106 54-55 0.5393404899296144 1106 56-57 0.41846752672131515 1106 58-59 0.5146804356927603 1106 60-61 0.7978475383362493 1106 62-63 0.7769521118172591 1106 64-65 0.7945848017949402 1106 66-67 0.6994197337389103 1106 68-69 0.6834248043570561 1106 70-71 0.5814281196447411 1106 72-73 0.38046051447378204 1106 74-75 0.6613756885296773 1106 76-77 0.6813369605488901 1106 78-79 0.5230160065092271 1106 80-81 0.7780816338544625 1106 82-83 0.5791806213209725 1106 84-85 0.687878168630796 1106 86-87 0.881055715842276 1106 88-89 0.6481566996511461 1106 90-91 0.5344819744709746 1106 92-93 0.7005434507669186 1106 94-95 0.6904392677821427 1106 96-97 0.9730719480318974 1106 98-99 0.9428751389187617 1106 100-101 0.9117161127497759 1107 1 0.2331555482828378 1107 2 0.30302458658727716 1107 3 0.23361333141502882 1107 4 0.40454118446583465 1107 5 0.30369627638310703 1107 6 0.413086247800571 1107 7 0.5192740340984514 1107 8 0.4611246809680978 1107 9 0.5597730053702961 1107 10-11 0.6221262852195295 1107 12-13 0.5415768848981202 1107 14-15 0.12299599035663533 1107 16-17 0.3673310072877882 1107 18-19 0.4887662649353217 1107 20-21 0.47518979631256997 1107 22-23 0.5333883443102252 1107 24-25 0.5434152168100965 1107 26-27 0.4458606979677455 1107 28-29 0.42166297101617545 1107 30-31 0.5768597455992293 1107 32-33 0.13440952638600834 1107 34-35 0.24614808066935723 1107 36-37 0.4057815573956276 1107 38-39 0.3538219366046391 1107 40-41 0.5660101967384605 1107 42-43 0.6921162126618512 1107 44-45 0.46357216975125937 1107 46-47 0.506923320917565 1107 48-49 0.5680008349152175 1107 50-51 0.5977940507942776 1107 52-53 0.5942019668200871 1107 54-55 0.656065451293685 1107 56-57 0.39068604961875764 1107 58-59 0.4982892271725987 1107 60-61 0.47546237453656914 1107 62-63 0.5181276163968391 1107 64-65 0.6062946063949397 1107 66-67 0.7472295931079422 1107 68-69 0.7977485351948523 1107 70-71 0.6895433367446699 1107 72-73 0.5233031132239852 1107 74-75 0.5957980079192424 1107 76-77 0.5721962692086073 1107 78-79 0.575479093076428 1107 80-81 0.5942718798269695 1107 82-83 0.473786192175794 1107 84-85 0.39770910401660586 1107 86-87 0.456814913636439 1107 88-89 0.32091404882967467 1107 90-91 0.14306480525520016 1107 92-93 0.5177762996861617 1107 94-95 0.6097785836016598 1107 96-97 0.4936268239108692 1107 98-99 0.4190722320916471 1107 100-101 0.3320285532007574 1108 1 0.10588409188825665 1108 2 0.17475633810094493 1108 3 0.19643103771296921 1108 4 0.31651508062874 1108 5 0.20962757059245973 1108 6 0.18462704987295098 1108 7 0.22041856577456542 1108 8 0.14926092757776388 1108 9 0.17479033697100022 1108 10-11 0.1940380778037465 1108 12-13 0.02289794154856395 1108 14-15 0.3116448244848016 1108 16-17 0.14413176218941715 1108 18-19 0.2080794823857488 1108 20-21 0.29207987276647884 1108 22-23 0.10644509219660137 1108 24-25 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2316 94-95 -3.8421313193960955 2316 96-97 -4.078283914398742 2316 98-99 -4.92642833185435 2316 100-101 -5.442828825033754 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5828.0 3 2460.0 4 2616.0 5 2784.0 6 3167.0 7 3782.0 8 4686.0 9 5906.0 10 7104.0 11 8863.0 12 10352.0 13 11751.0 14 12926.0 15 15284.0 16 17676.0 17 21116.0 18 18170.0 19 15299.0 20 16258.0 21 17319.0 22 19126.0 23 21553.0 24 24763.0 25 28213.0 26 32900.0 27 38127.0 28 44881.0 29 53596.0 30 64247.0 31 78229.0 32 96934.0 33 121500.0 34 158572.0 35 211718.0 36 285474.0 37 336867.0 38 213219.0 39 24562.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.44872660963521 17.583537537823595 13.902219016727436 23.065516835813757 2 16.19066550090324 19.61913750678584 38.87498645261721 25.315210539693712 3 20.46745369447833 23.471103702011636 28.60808833893066 27.45335426457937 4 13.404753155906546 15.648105483976957 35.770521412337445 35.17661994777905 5 14.792371311823649 36.89449936855368 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26.630313159015657 23.618610851186716 52-53 24.23816613157536 25.995377957996325 26.093057533512965 23.67339837691535 54-55 23.721883875131454 25.739120461715803 26.330771593358847 24.20822406979389 56-57 23.987190296503815 25.94914994788161 25.99871708584105 24.064942669773522 58-59 24.442328280945734 25.481543290662863 26.04819345028228 24.027934978109126 60-61 23.85995741028898 25.659620541420004 26.751868766929704 23.728553281361318 62-63 23.763448698136862 25.8071891613455 26.68583743962057 23.743524700897066 64-65 23.59978035007921 26.22510715222906 26.27950938371675 23.895603113974985 66-67 23.647545057124727 26.0164721601247 26.076643102954282 24.259339679796295 68-69 23.75220108296685 25.780746370096967 26.392504018100656 24.074548528835525 70-71 24.010524519368452 25.756872225255638 26.024093690879802 24.208509564496108 72-73 23.84303490475826 25.774843835623095 26.33724215107696 24.044879108541682 74-75 23.590098442463912 26.54208419585634 25.986934128397866 23.880883233281878 76-77 23.910761543974807 25.917178286125647 25.925320126215535 24.24674004368401 78-79 23.879202958517162 26.074752541199373 26.090408643724476 23.95563585655898 80-81 24.04333673292217 26.155993951747234 25.836996767229326 23.963672548101265 82-83 23.944685700505826 26.106898583644327 26.05353972232972 23.89487599352012 84-85 23.8194215097961 26.285938138297084 25.852421643697998 24.042218708208818 86-87 23.943587010489217 26.301175975388336 25.692713395095794 24.06252361902665 88-89 23.77407306956122 26.2778861679552 25.4177396322122 24.530301130271383 90-91 23.832167682585194 26.053041666636283 25.686902635894747 24.427888014883774 92-93 24.08424056024274 26.049464777641813 25.87552510605411 23.990769556061338 94-95 23.75816165179755 25.912529109051018 26.072961503758446 24.256347735392985 96-97 23.496548667146868 25.782005632170836 26.310811454478472 24.410634246203823 98-99 23.56652006196099 26.14881139335407 25.621068486730213 24.663600057954724 100-101 23.70216974897552 25.85540939490268 25.718836634469827 24.723584221651972 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 195.0 1 155.5 2 173.5 3 236.5 4 222.5 5 217.5 6 347.5 7 632.0 8 997.0 9 1261.5 10 1206.5 11 1089.0 12 1173.0 13 1293.5 14 1386.5 15 1528.0 16 1686.0 17 1805.5 18 1958.5 19 2195.5 20 2506.0 21 2889.5 22 3378.5 23 4041.5 24 5001.5 25 6127.5 26 7636.5 27 9746.5 28 11800.5 29 13849.5 30 16385.5 31 19782.5 32 23815.0 33 26124.5 34 27395.0 35 29512.5 36 33244.0 37 37202.5 38 40177.0 39 42582.5 40 45213.0 41 48718.5 42 52113.5 43 55761.0 44 59602.5 45 64239.0 46 68754.5 47 76865.5 48 92540.0 49 94551.0 50 83291.5 51 78789.5 52 79019.0 53 81469.0 54 83301.0 55 80481.0 56 74885.0 57 69130.0 58 65426.0 59 59922.5 60 51169.0 61 44165.5 62 38066.0 63 30135.5 64 21880.0 65 16598.0 66 12916.0 67 10416.0 68 8147.0 69 6312.5 70 5075.0 71 3747.0 72 2957.5 73 2564.0 74 2023.5 75 1522.0 76 1118.5 77 707.5 78 462.5 79 294.5 80 184.0 81 127.5 82 88.5 83 57.0 84 43.5 85 28.5 86 16.0 87 12.0 88 13.5 89 13.5 90 9.0 91 6.5 92 8.0 93 5.5 94 3.0 95 3.0 96 2.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012440264861014058 2 0.011711343091814016 3 0.014772814522454191 4 0.020506999106827857 5 0.031683799567895174 6 0.05000403336712291 7 0.00607434807666702 8 0.025220693214321466 9 0.0350854344908287 10-11 0.04098970082134905 12-13 0.031149256937148477 14-15 0.027796216798828283 16-17 0.03173239435250851 18-19 0.024613258406654764 20-21 0.05632135536685661 22-23 0.014238271891707495 24-25 0.0029885792537201737 26-27 0.0013363565768667444 28-29 4.8594784613336165E-5 30-31 0.002648415761426821 32-33 0.0019923861691467826 34-35 0.0 36-37 3.6446088460002115E-4 38-39 7.289217692000423E-5 40-41 0.0013849513614800804 42-43 0.004203448869053578 44-45 0.0017494122460801017 46-47 0.0011662748307200677 48-49 5.831374153600339E-4 50-51 0.003766095807533552 52-53 0.004154854084440242 54-55 0.0057341845843736665 56-57 9.23300907653387E-4 58-59 0.006171537645893691 60-61 0.0029156870768001692 62-63 6.803269845867062E-4 64-65 6.803269845867062E-4 66-67 0.01761560942233436 68-69 0.043346547875095856 70-71 0.05408599527464314 72-73 0.014894301483987533 74-75 0.03122214911406848 76-77 0.027188781991161582 78-79 0.054814917043843184 80-81 0.08275691819651147 82-83 0.04888635332101618 84-85 0.017372635499267677 86-87 0.021163028699107897 88-89 0.021964842645227942 90-91 0.04026077905214901 92-93 0.024856232329721444 94-95 0.013096294453294095 96-97 0.017882880737707704 98-99 0.051316092551682985 100-101 0.06905318893555068 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2057834.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.25523703378793 #Duplication Level Percentage of deduplicated Percentage of total 1 77.59753643473833 42.87670268939462 2 12.557106096131875 13.876917476003806 3 4.067632913255917 6.742740623651793 4 1.8639127587023532 4.119637651696004 5 0.9875776032981226 2.7284417279748974 6 0.6235698501149933 2.0673299925136543 7 0.4239695455161223 1.6398576412820474 8 0.2908287791331376 1.2855850501798942 9 0.2192317661420254 1.090233288316222 >10 1.2381609801299895 12.355647194489624 >50 0.0829648606272128 3.122820772665915 >100 0.04158310571925844 4.145884261050428 >500 0.003714371232984779 1.3481723698156098 >1k 0.0020340604371107043 2.005912777141643 >5k 1.7687482061832212E-4 0.5941164838239201 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6737 0.3273830639400457 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5421 0.2634323273888953 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4345 0.2111443391449456 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4289 0.2084230312065988 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2916 0.14170239193248824 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2655 0.1290191531484075 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2364 0.11487807082592669 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2119 0.10297234859565933 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 4.859478461333616E-5 0.0 0.0 0.0 8 0.0 4.859478461333616E-5 0.0 0.0 0.0 9 0.0 4.859478461333616E-5 0.0 0.0 0.0 10-11 0.0 4.859478461333616E-5 0.0 0.0 0.0 12-13 0.0 4.859478461333616E-5 0.0 0.0 0.0 14-15 0.0 9.718956922667232E-5 0.0 4.859478461333616E-5 0.0 16-17 0.0 1.4578435384000847E-4 0.0 9.718956922667232E-5 0.0 18-19 0.0 1.7008174614667655E-4 0.0 1.9437913845334463E-4 0.0 20-21 0.0 1.9437913845334463E-4 0.0 2.672713153733489E-4 0.0 22-23 0.0 1.9437913845334463E-4 0.0 3.644608846000212E-4 0.0 24-25 0.0 1.9437913845334463E-4 0.0 5.102452384400296E-4 0.0 26-27 0.0 1.9437913845334463E-4 0.0 7.532191615067104E-4 2.429739230666808E-5 28-29 0.0 2.1867653076001271E-4 0.0 0.00126346439994674 4.859478461333616E-5 30-31 0.0 2.429739230666808E-4 0.0 0.002697010546040157 4.859478461333616E-5 32-33 0.0 2.429739230666808E-4 0.0 0.00544261587669365 1.2148696153334038E-4 34-35 0.0 2.429739230666808E-4 0.0 0.008649871661173836 1.4578435384000847E-4 36-37 0.0 2.429739230666808E-4 0.0 0.011954317014880695 1.4578435384000847E-4 38-39 0.0 2.672713153733489E-4 0.0 0.015210167583974216 1.4578435384000847E-4 40-41 0.0 2.9156870768001693E-4 0.0 0.01798007030693438 1.4578435384000847E-4 42-43 0.0 2.9156870768001693E-4 0.0 0.021041541737574557 1.4578435384000847E-4 44-45 0.0 2.9156870768001693E-4 0.0 0.024078715775908067 1.7008174614667655E-4 46-47 0.0 2.9156870768001693E-4 0.0 0.030347442991028428 1.9437913845334463E-4 48-49 0.0 2.9156870768001693E-4 0.0 0.03741798415226884 1.9437913845334463E-4 50-51 0.0 2.9156870768001693E-4 0.0 0.04337084526740252 1.9437913845334463E-4 52-53 0.0 2.9156870768001693E-4 0.0 0.05284682826700307 2.672713153733489E-4 54-55 0.0 2.9156870768001693E-4 0.0 0.07065681682779078 2.9156870768001693E-4 56-57 0.0 4.130556692133573E-4 0.0 0.10532919564940613 2.9156870768001693E-4 58-59 0.0 4.6165045382669354E-4 0.0 0.1430144511170483 2.9156870768001693E-4 60-61 0.0 4.859478461333616E-4 0.0 0.17936335000782377 3.401634922933531E-4 62-63 0.0 4.859478461333616E-4 0.0 0.22496955536743973 3.401634922933531E-4 64-65 0.0 4.859478461333616E-4 0.0 0.28150958726505637 3.401634922933531E-4 66-67 0.0 4.859478461333616E-4 0.0 0.3700735822228615 3.8875827690668926E-4 68-69 0.0 4.859478461333616E-4 0.0 0.476690539664521 3.8875827690668926E-4 70-71 0.0 4.859478461333616E-4 0.0 0.5710373139913132 3.8875827690668926E-4 72-73 0.0 4.859478461333616E-4 0.0 0.6670606083872654 4.130556692133573E-4 74-75 0.0 4.859478461333616E-4 0.0 0.7726813727443516 4.3735306152002543E-4 76-77 0.0 4.859478461333616E-4 0.0 0.8956747726007055 4.3735306152002543E-4 78-79 0.0 4.859478461333616E-4 0.0 0.9978453072502447 4.3735306152002543E-4 80-81 0.0 4.859478461333616E-4 0.0 1.1031502055073443 4.3735306152002543E-4 82-83 2.429739230666808E-5 4.859478461333616E-4 0.0 1.2237381635253377 4.3735306152002543E-4 84-85 4.859478461333616E-5 4.859478461333616E-4 0.0 1.4021053204485883 4.3735306152002543E-4 86-87 4.859478461333616E-5 4.859478461333616E-4 0.0 1.6335379821696017 4.3735306152002543E-4 88-89 4.859478461333616E-5 4.859478461333616E-4 0.0 1.9049398542350842 4.3735306152002543E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11100 0.0 24.566433 1 GTATAGG 525 0.0 24.431921 1 TACATGG 10970 0.0 24.251863 2 ACATGGG 11200 0.0 23.200714 3 TACCGTA 130 2.9379415E-4 21.928217 7 GAGTACT 6345 0.0 21.4538 12-13 GTACTTT 6745 0.0 20.146791 14-15 GTATCAA 18345 0.0 20.069527 1 CATGGGG 5755 0.0 19.811096 4 AGTACTT 6715 0.0 19.457989 12-13 ATGGGAG 2480 0.0 18.964294 5 CATGGGA 5550 0.0 18.830954 4 AGAGTAC 13800 0.0 17.955261 10-11 TACTTTT 7230 0.0 17.645248 14-15 TCAACGC 20750 0.0 17.6056 4 ATCAACG 20885 0.0 17.55961 3 CAACGCA 20850 0.0 17.52159 5 TATCAAC 20910 0.0 17.517176 2 ACTTTTT 8045 0.0 17.452324 16-17 GTACACG 440 5.456968E-12 17.275097 1 >>END_MODULE