FastQCFastQC Report
Thu 26 May 2016
SRR936503_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936503_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2057834
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT76410.37131274923050156No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63840.31022910497153805No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57660.2801975280804963No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT37240.18096697790006386No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30970.15049804794750207No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT27000.13120591845600763No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC26110.12688098262542072No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC21100.10253499553413928No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT20730.10073698850344587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC1205.3830936E-627.709193
TACATGG102350.023.1623742
GTACATG105350.023.0450611
ACATGGG102800.022.2258833
GAGTACT85000.021.98985312-13
GTACTTT87300.020.83970614-15
AGTACTT87900.019.80530712-13
GTATCAA189400.019.5912671
TAAGGTG8750.019.5425115
TAAGGTA5650.019.3364124
CATGGGG55700.018.8466244
GTAAGGT8350.018.772584
AGAGTAC151900.018.57439210-11
TACTTTT93450.018.34995314-15
ACTTTTT101100.018.27569216-17
AGGCGAC6250.018.239685
GCGACAG6400.017.8100227
GGCGACA6700.017.7214156
GTGTAAG8150.017.4886041
GTATAGC6000.017.4205931
Produced by FastQC (version 0.11.5)