FastQCFastQC Report
Thu 26 May 2016
SRR936502_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936502_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2042560
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT66640.32625724580918064No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55700.27269700767664107No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44130.21605240482531723No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT43920.21502428325238915No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT28700.14050994830017233No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC26930.13184435218549273No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24090.11794023186589378No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCGT1153.8554754E-628.9236538
GTACCGT2202.1609594E-923.7598786
TACATGG98750.022.713242
GTACATG100650.022.7093941
ACATGGG104050.020.7799033
TAAGGTA5800.020.4826554
GAGTACT61650.020.15392512-13
GTACTTT62750.019.68608514-15
AGTACTT63250.018.81777212-13
GTAGCCC9350.018.804613
GTATCAA174150.018.745951
GTGTAAG7450.018.4976231
TCGCGTG1300.007549118.2759349
CATGGGG52850.018.0727924
TACCTGG16350.017.7290822
GTACCTG17550.017.3291421
TAGACCG1650.001460266317.2799114
TACTTTT70100.016.97811514-15
TAGCCCT10650.016.9554064
AGAGTAC133200.016.85522510-11
Produced by FastQC (version 0.11.5)