##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936502_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2042560 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.15201854535485 31.0 31.0 34.0 28.0 34.0 2 31.182727557574808 31.0 31.0 34.0 28.0 34.0 3 31.230266430361898 31.0 31.0 34.0 27.0 34.0 4 34.87135849130503 37.0 35.0 37.0 32.0 37.0 5 34.78064732492558 37.0 35.0 37.0 32.0 37.0 6 34.90368067523108 37.0 35.0 37.0 32.0 37.0 7 34.721992989190035 37.0 35.0 37.0 31.0 37.0 8 34.70097524674918 37.0 35.0 37.0 31.0 37.0 9 36.169426601911326 39.0 35.0 39.0 31.0 39.0 10-11 36.03793646208679 39.0 35.0 39.0 30.0 39.0 12-13 35.92976803618988 39.0 35.0 39.0 30.0 39.0 14-15 36.96098278630738 39.0 36.0 41.0 31.0 41.0 16-17 36.8821814291869 39.0 36.0 41.0 30.5 41.0 18-19 36.82894407018644 39.0 36.0 41.0 30.0 41.0 20-21 36.71710304715651 39.0 36.0 41.0 30.0 41.0 22-23 36.606979966316786 39.0 36.0 41.0 30.0 41.0 24-25 36.44142154942817 39.0 36.0 40.5 30.0 41.0 26-27 36.21561251566661 39.0 35.0 40.0 29.0 41.0 28-29 36.333547117342945 39.0 35.0 40.0 30.0 41.0 30-31 36.2614305087733 39.0 35.0 40.0 29.5 41.0 32-33 36.12249505522482 39.0 35.0 40.0 29.0 41.0 34-35 35.94289567993107 38.0 35.0 40.0 28.5 41.0 36-37 35.69048473484255 38.0 35.0 40.0 27.0 41.0 38-39 35.476975706955976 38.0 34.5 40.0 27.0 41.0 40-41 35.32397334717218 38.0 34.0 40.0 26.5 41.0 42-43 35.046161679461065 38.0 34.0 40.0 25.5 41.0 44-45 34.758881256854146 38.0 33.5 40.0 24.5 41.0 46-47 34.41906749373335 38.0 33.0 40.0 24.0 41.0 48-49 34.14352528199906 38.0 33.0 40.0 23.0 41.0 50-51 33.41931473249256 37.0 32.0 39.5 22.5 40.5 52-53 33.58544889746201 37.5 33.0 39.5 23.0 40.5 54-55 33.91045648597838 38.0 33.0 40.0 22.5 41.0 56-57 33.7982791203196 37.5 33.0 40.0 22.0 41.0 58-59 33.52945592785524 37.0 33.0 40.0 22.0 41.0 60-61 33.162033183847726 36.5 32.0 40.0 20.5 41.0 62-63 32.819469685101055 36.0 32.0 39.0 20.0 41.0 64-65 32.47622419904434 35.5 31.5 39.0 19.0 41.0 66-67 32.07535886338712 35.0 31.0 39.0 18.0 40.0 68-69 31.682904786150715 35.0 31.0 38.0 16.0 40.0 70-71 31.277087821165594 35.0 30.5 37.0 13.0 39.5 72-73 30.86386617773774 35.0 30.0 37.0 9.5 39.0 74-75 30.447910220507598 34.5 30.0 36.0 7.5 39.0 76-77 30.04235640568698 34.0 29.5 36.0 7.0 38.5 78-79 29.611050838163873 34.0 29.0 35.0 2.0 37.0 80-81 29.216695715180947 34.0 29.0 35.0 2.0 37.0 82-83 28.828824857042143 34.0 29.0 35.0 2.0 36.0 84-85 28.460928932320225 34.0 28.0 35.0 2.0 36.0 86-87 28.133811736252547 34.0 27.0 35.0 2.0 35.5 88-89 27.758146884302054 33.0 26.5 35.0 2.0 35.0 90-91 27.401286865502115 33.0 26.0 35.0 2.0 35.0 92-93 27.0314242910857 33.0 25.0 35.0 2.0 35.0 94-95 26.589660768839103 33.0 25.0 35.0 2.0 35.0 96-97 25.98192415400282 33.0 24.0 35.0 2.0 35.0 98-99 25.290091845527183 32.0 20.0 35.0 2.0 35.0 100-101 23.173596858843805 30.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.44651306246770517 1101 2 0.48704880399753137 1101 3 0.5843312194995036 1101 4 0.49093141761126446 1101 5 0.5456707883727887 1101 6 0.531032016584021 1101 7 0.5479578772063647 1101 8 0.6588331703511372 1101 9 0.9255668547124927 1101 10-11 0.6331653472819809 1101 12-13 0.7669494281134135 1101 14-15 0.8965370442236207 1101 16-17 0.9270540581081974 1101 18-19 1.099282133320166 1101 20-21 1.1117513603314038 1101 22-23 0.9684520273052257 1101 24-25 0.8100279096992153 1101 26-27 1.054867233792308 1101 28-29 0.9572442484384069 1101 30-31 0.8052621751156437 1101 32-33 0.817028919328763 1101 34-35 0.8486636577773581 1101 36-37 1.021683386012306 1101 38-39 0.8878249226656934 1101 40-41 0.8081670899198485 1101 42-43 0.6903069454774169 1101 44-45 0.6677795047808956 1101 46-47 0.7102618376317267 1101 48-49 0.56508061182803 1101 50-51 0.6969781570935822 1101 52-53 0.8487458223344717 1101 54-55 0.7875194960796321 1101 56-57 0.6636120810515109 1101 58-59 0.7625842209349756 1101 60-61 0.752326127316941 1101 62-63 0.8163299705631673 1101 64-65 0.9942962133601938 1101 66-67 0.8505767086385703 1101 68-69 0.6943027710111913 1101 70-71 0.7016780519831087 1101 72-73 0.8272892129335858 1101 74-75 0.6076507245854792 1101 76-77 0.6868286633142837 1101 78-79 0.7144880876156812 1101 80-81 0.6428777593116273 1101 82-83 0.6822383317790006 1101 84-85 0.5834678418246639 1101 86-87 0.7282440223658817 1101 88-89 0.6281708591295505 1101 90-91 0.5305019948290592 1101 92-93 0.24190000041311066 1101 94-95 0.19833334619227116 1101 96-97 0.16498748224269377 1101 98-99 0.06264671663117838 1101 100-101 0.46685879540992303 1102 1 0.40018211206517407 1102 2 0.4153098444142671 1102 3 0.47356804833212607 1102 4 0.4170773494226623 1102 5 0.4384480587381461 1102 6 0.49803800099844864 1102 7 0.29060821816462123 1102 8 0.34470978443767564 1102 9 0.7132273477342324 1102 10-11 0.5973447011666551 1102 12-13 0.6780915943905583 1102 14-15 0.6501875522846134 1102 16-17 0.6616910979938666 1102 18-19 0.6115744852797249 1102 20-21 0.36058641009215364 1102 22-23 0.10880459118769181 1102 24-25 0.38843190307309783 1102 26-27 0.4429728762946965 1102 28-29 0.6753507073788185 1102 30-31 0.48006384553258385 1102 32-33 0.41147744596332814 1102 34-35 0.4072194323214333 1102 36-37 0.32402869961988756 1102 38-39 0.33697494963867314 1102 40-41 0.12507361387010008 1102 42-43 0.308524882544539 1102 44-45 0.3458457827620123 1102 46-47 0.34447432974393877 1102 48-49 0.4912120461417757 1102 50-51 0.5358213025622334 1102 52-53 0.37054891426920733 1102 54-55 0.364226900714705 1102 56-57 0.569987241191626 1102 58-59 0.5433366563238238 1102 60-61 0.07078197595085811 1102 62-63 0.2939778812829914 1102 64-65 0.47224208580815485 1102 66-67 0.4254146991022765 1102 68-69 0.5233486601694217 1102 70-71 0.5027124865921451 1102 72-73 0.5680106236774556 1102 74-75 0.40557659048667816 1102 76-77 0.407107076311366 1102 78-79 0.3134808167390908 1102 80-81 0.4458174703230817 1102 82-83 0.43048699591089346 1102 84-85 0.37006658389968194 1102 86-87 0.2234728195848028 1102 88-89 -0.025871920696953765 1102 90-91 -0.08332205590912878 1102 92-93 0.07885618210795542 1102 94-95 0.15710704101689288 1102 96-97 -0.12982905192447447 1102 98-99 -0.20165868918048702 1102 100-101 0.24871494818607687 1103 1 0.5162716643907892 1103 2 0.5621230894961684 1103 3 0.5408379538573413 1103 4 0.5878141577530442 1103 5 0.6197323380795154 1103 6 0.41387246364765673 1103 7 0.5546296683806915 1103 8 0.37417620449664923 1103 9 0.6422898313761181 1103 10-11 0.6628545945553626 1103 12-13 0.7837878055115581 1103 14-15 0.8166633742617719 1103 16-17 0.9978266372252378 1103 18-19 0.7368780192144655 1103 20-21 0.7374703803984204 1103 22-23 0.7810908160543519 1103 24-25 0.7956554533320173 1103 26-27 0.6130305198729147 1103 28-29 0.7194924176186603 1103 30-31 0.6005383423811637 1103 32-33 0.7492561882641127 1103 34-35 0.7712082390077555 1103 36-37 0.8630752807493565 1103 38-39 0.7064156135406918 1103 40-41 0.526274105728362 1103 42-43 0.6948216764506938 1103 44-45 0.6929828216085383 1103 46-47 1.0612402114149049 1103 48-49 1.0888052946746356 1103 50-51 0.3336477087786065 1103 52-53 0.7994197050229701 1103 54-55 0.9977256085446982 1103 56-57 0.9930058249810543 1103 58-59 1.1216005488478586 1103 60-61 1.245938989632542 1103 62-63 1.1308415384440238 1103 64-65 1.1051914032748442 1103 66-67 0.9984253340589078 1103 68-69 1.0235456845917277 1103 70-71 1.094857391875017 1103 72-73 1.1371435368858904 1103 74-75 0.9278407137207303 1103 76-77 0.9149321932658374 1103 78-79 1.0022110682517926 1103 80-81 1.0028810018537762 1103 82-83 1.1914725009235063 1103 84-85 0.9134244374434068 1103 86-87 0.6976034887020397 1103 88-89 0.8406293714730602 1103 90-91 0.9680967138054299 1103 92-93 0.961879050512124 1103 94-95 0.8837672807614432 1103 96-97 0.9643492277930115 1103 98-99 0.7699696728364991 1103 100-101 0.8186439563850065 1104 1 0.3335772880531245 1104 2 0.43676050589792936 1104 3 0.31893030046871473 1104 4 0.3003303395530139 1104 5 0.38365888661749636 1104 6 0.2715245540209281 1104 7 0.38156323119574864 1104 8 0.3759353377085617 1104 9 0.5380252547736077 1104 10-11 0.584165375992562 1104 12-13 0.4919311920238201 1104 14-15 0.4764210923775991 1104 16-17 0.32713646722823597 1104 18-19 0.5355233382694777 1104 20-21 0.4272726935148512 1104 22-23 0.40744725891452305 1104 24-25 0.427705432374502 1104 26-27 0.5265763556327414 1104 28-29 0.33984884378700286 1104 30-31 0.21246493172139225 1104 32-33 0.03173825382219775 1104 34-35 0.15849226529924465 1104 36-37 0.21018810452445535 1104 38-39 0.17674820748786857 1104 40-41 -0.016538346746294508 1104 42-43 0.1583778518706751 1104 44-45 0.007072718230489272 1104 46-47 0.0694994206673698 1104 48-49 0.013510312980002936 1104 50-51 -0.14654256878794314 1104 52-53 0.02769575447529604 1104 54-55 0.05513344183413693 1104 56-57 0.23943029631375623 1104 58-59 0.1903363376109155 1104 60-61 0.2782991121134657 1104 62-63 0.16839513775854442 1104 64-65 0.17140927614246237 1104 66-67 0.11402482106339562 1104 68-69 0.23458834890422864 1104 70-71 0.21593249397336223 1104 72-73 -0.08412171295367799 1104 74-75 -0.24095238365197602 1104 76-77 -0.06680346938118831 1104 78-79 0.39443425283002753 1104 80-81 0.4508694974749581 1104 82-83 0.23711375459106776 1104 84-85 0.43008079536071264 1104 86-87 0.3729052398602626 1104 88-89 0.21982782989000427 1104 90-91 0.13071313930608852 1104 92-93 0.0029291241416302682 1104 94-95 0.061824110872020555 1104 96-97 0.3053607247532426 1104 98-99 -0.022325796630436656 1104 100-101 0.255494366076654 1105 1 0.19513117582739525 1105 2 0.2745158655645561 1105 3 0.3654518743566868 1105 4 0.35497777991405854 1105 5 0.4448230131272837 1105 6 0.40423835442685885 1105 7 0.4165505491297097 1105 8 0.09886970633215242 1105 9 0.44246366341916143 1105 10-11 0.45244057907184043 1105 12-13 0.40177172054656296 1105 14-15 0.5316148094763307 1105 16-17 0.4784589421134342 1105 18-19 0.36910749768166085 1105 20-21 0.11088883886759504 1105 22-23 -0.13764797562957654 1105 24-25 0.07231153360281439 1105 26-27 0.035126305362702226 1105 28-29 0.018070955595831606 1105 30-31 -0.04760075625935656 1105 32-33 -0.0988573765029912 1105 34-35 -0.3216798208579448 1105 36-37 -0.22789888465513286 1105 38-39 -0.313691051121161 1105 40-41 -0.5330045921449411 1105 42-43 -0.413300754117337 1105 44-45 -0.29109105517608214 1105 46-47 -0.3232618734694057 1105 48-49 -0.24397763164478903 1105 50-51 -0.1730394613897559 1105 52-53 0.026439677371207893 1105 54-55 -0.04298006153381806 1105 56-57 0.10122475250335583 1105 58-59 -0.011846028971326916 1105 60-61 -0.15480781886248707 1105 62-63 -0.08649642001965674 1105 64-65 0.07851011002312447 1105 66-67 -0.2709765131815942 1105 68-69 -0.07562338724051543 1105 70-71 0.04356960449287328 1105 72-73 0.0349583049081339 1105 74-75 0.05326772993405271 1105 76-77 0.19492440933099786 1105 78-79 0.16470781348516184 1105 80-81 0.20516176810835773 1105 82-83 0.2128680073285203 1105 84-85 -0.15366890926350152 1105 86-87 0.10840958895985153 1105 88-89 0.08727283608503811 1105 90-91 0.04830149822068819 1105 92-93 0.17817456681896005 1105 94-95 0.1901962241633477 1105 96-97 0.22043266167265685 1105 98-99 0.19577451702944515 1105 100-101 0.43182113121089927 1106 1 0.12627160054326225 1106 2 0.15673805243724104 1106 3 0.04684007724359418 1106 4 0.31726004332733027 1106 5 0.33530484059237153 1106 6 0.4058942593684165 1106 7 0.6014317282842612 1106 8 0.5368264057987702 1106 9 0.48295103634308845 1106 10-11 0.3652698324056374 1106 12-13 0.5284770596082851 1106 14-15 0.4059264324487586 1106 16-17 0.4176822597294816 1106 18-19 0.37339440248933187 1106 20-21 0.4246291381809755 1106 22-23 0.4407293524136975 1106 24-25 0.1823525479047987 1106 26-27 0.3671570082043232 1106 28-29 0.1535903706670183 1106 30-31 0.08561740385903249 1106 32-33 0.15948599830860388 1106 34-35 0.1912926864804021 1106 36-37 0.3041730887726217 1106 38-39 0.2773513537432919 1106 40-41 0.29459526698714456 1106 42-43 0.45446766928882454 1106 44-45 0.27346935342923473 1106 46-47 0.2817907686213772 1106 48-49 0.2888633047114624 1106 50-51 0.5530311104287975 1106 52-53 0.38897703189128663 1106 54-55 0.5096304355999948 1106 56-57 0.4100228549401308 1106 58-59 0.5238731330855373 1106 60-61 0.6266865210136032 1106 62-63 0.4858706027494222 1106 64-65 0.50202497649245 1106 66-67 0.4555518351598309 1106 68-69 0.38519927163688195 1106 70-71 0.3483088066449582 1106 72-73 0.3690266877065156 1106 74-75 0.2834113418526769 1106 76-77 0.2802630556999617 1106 78-79 0.3289204489125588 1106 80-81 0.36088057225114767 1106 82-83 0.29999014837936144 1106 84-85 0.3648149225204165 1106 86-87 0.465427665388205 1106 88-89 0.41756655098128803 1106 90-91 0.44620750203048587 1106 92-93 0.30957819094201966 1106 94-95 0.3499100784870137 1106 96-97 0.5441947760699506 1106 98-99 0.6692824682453171 1106 100-101 0.8077151983653046 1107 1 0.40449965785571607 1107 2 0.4546288708238606 1107 3 0.49272665244693314 1107 4 0.507328407837889 1107 5 0.46788862729586356 1107 6 0.423148238898051 1107 7 0.520241620930399 1107 8 0.6148550468104403 1107 9 0.7728649649583303 1107 10-11 0.6946395646818857 1107 12-13 0.7321297680848531 1107 14-15 0.6963066880477768 1107 16-17 0.6115264107076896 1107 18-19 0.9059369650791353 1107 20-21 1.0737226593859575 1107 22-23 0.9858617926054478 1107 24-25 0.9302489056331069 1107 26-27 0.9161551005786706 1107 28-29 0.73013361224222 1107 30-31 0.8768345042109829 1107 32-33 0.9128998256466119 1107 34-35 0.8850298544646265 1107 36-37 0.9449792317836838 1107 38-39 0.9903833801723678 1107 40-41 0.7694997906085135 1107 42-43 0.9506118833156805 1107 44-45 1.094899045632033 1107 46-47 0.8755629081576259 1107 48-49 0.9974958338179718 1107 50-51 1.0686266158117022 1107 52-53 0.9384428776893259 1107 54-55 1.025928819253373 1107 56-57 1.104315621273102 1107 58-59 0.9920795703236109 1107 60-61 1.0310388507228225 1107 62-63 1.0362295188072395 1107 64-65 0.9120625013719419 1107 66-67 0.9073728759094166 1107 68-69 1.076773362352757 1107 70-71 1.0940809575927233 1107 72-73 0.9897951536845007 1107 74-75 0.8564694340938921 1107 76-77 0.9812926600340575 1107 78-79 0.9757057525873556 1107 80-81 1.2735403691831593 1107 82-83 1.2556151477850115 1107 84-85 1.3163706454449233 1107 86-87 1.219628768691937 1107 88-89 1.1815945177623561 1107 90-91 1.1144767739470218 1107 92-93 0.9270179404972083 1107 94-95 1.10479848809209 1107 96-97 1.2428054949100797 1107 98-99 1.1637180449882614 1107 100-101 1.3746661069599817 1108 1 0.20021501532605512 1108 2 0.0942479210892202 1108 3 -0.04550725235731434 1108 4 0.026264749497840967 1108 5 0.21392213753509992 1108 6 0.15557342437139 1108 7 0.3781704325117161 1108 8 0.0634504509617102 1108 9 0.48215113573273527 1108 10-11 0.06792028934774663 1108 12-13 0.24645706253594568 1108 14-15 0.3037179803870984 1108 16-17 0.34425029856230793 1108 18-19 0.3885080284596114 1108 20-21 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-1.2640582128713405 2315 18-19 -1.2630178811858386 2315 20-21 -1.1391838409715689 2315 22-23 -0.570580376174739 2315 24-25 -0.947526460281054 2315 26-27 -0.9618450854793821 2315 28-29 -1.3411950246454225 2315 30-31 -1.2621919014393441 2315 32-33 -1.0150920607631235 2315 34-35 -0.8182308128635825 2315 36-37 -0.8197166180273427 2315 38-39 -1.0779678718888661 2315 40-41 -1.201370723933202 2315 42-43 -1.2497990860832502 2315 44-45 -1.430064654807211 2315 46-47 -1.8680262975569022 2315 48-49 -1.8342502297774033 2315 50-51 -1.7527986982980615 2315 52-53 -1.881024266647941 2315 54-55 -1.6098433946185295 2315 56-57 -1.72192485693256 2315 58-59 -1.746730953265633 2315 60-61 -1.6906319870491515 2315 62-63 -1.8324454195382316 2315 64-65 -1.7883785656527351 2315 66-67 -1.5001758822443065 2315 68-69 -1.623904837480886 2315 70-71 -1.3736397948114316 2315 72-73 -1.190130733192209 2315 74-75 -0.9566069810417481 2315 76-77 -1.31111352657852 2315 78-79 -1.1756050446166668 2315 80-81 -1.2100149265396958 2315 82-83 -1.333248092251761 2315 84-85 -1.2507931184729486 2315 86-87 -1.2843514143016286 2315 88-89 -1.3355246469464461 2315 90-91 -1.3207175655104209 2315 92-93 -1.1129895565477526 2315 94-95 -1.0284391408716758 2315 96-97 -1.095781850223954 2315 98-99 -1.1605355338930572 2315 100-101 -1.2654169077641413 2316 1 -3.51634082687929 2316 2 -2.9233068838293335 2316 3 -2.9601432798711222 2316 4 -2.544751460193602 2316 5 -5.606937359352237 2316 6 -6.009240226222243 2316 7 -4.966980063198939 2316 8 -4.317099427587294 2316 9 -7.781918451836642 2316 10-11 -6.794934354070346 2316 12-13 -6.294557472048169 2316 14-15 -5.131681493862295 2316 16-17 -5.099584929379123 2316 18-19 -4.6573655742940545 2316 20-21 -4.8608287674106805 2316 22-23 -4.414810144754647 2316 24-25 -3.886366462299762 2316 26-27 -4.130382860484339 2316 28-29 -3.917978663046746 2316 30-31 -3.7349600584666973 2316 32-33 -2.7782149441690223 2316 34-35 -2.6188988778310573 2316 36-37 -2.5140854335699743 2316 38-39 -2.7822286649960333 2316 40-41 -2.3737653553978433 2316 42-43 -2.020823848276393 2316 44-45 -2.452937575628752 2316 46-47 -2.2196752396514157 2316 48-49 -2.7903001213590564 2316 50-51 -2.964545338721571 2316 52-53 -2.716647350184015 2316 54-55 -2.3819210155406623 2316 56-57 -2.0281084659234523 2316 58-59 -2.1990472207161105 2316 60-61 -2.5462626494278098 2316 62-63 -2.3696576671566802 2316 64-65 -2.4269500016824317 2316 66-67 -3.1707950110341656 2316 68-69 -2.562161199009953 2316 70-71 -2.2210862208346214 2316 72-73 -2.237823167053538 2316 74-75 -2.422533731658671 2316 76-77 -3.1708891676041624 2316 78-79 -3.104197729249801 2316 80-81 -3.2881817472195785 2316 82-83 -3.116671244876251 2316 84-85 -3.1612306247813997 2316 86-87 -3.3541437661966 2316 88-89 -3.577498556933115 2316 90-91 -3.565712788490238 2316 92-93 -3.8967744662107364 2316 94-95 -3.953416865513443 2316 96-97 -4.451276554605585 2316 98-99 -4.7725188064912345 2316 100-101 -5.313902248664132 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5912.0 3 2554.0 4 2508.0 5 2812.0 6 3309.0 7 3644.0 8 4526.0 9 5631.0 10 6835.0 11 8418.0 12 9667.0 13 11157.0 14 12349.0 15 14225.0 16 16489.0 17 19563.0 18 17857.0 19 14171.0 20 14973.0 21 16281.0 22 18491.0 23 20437.0 24 23764.0 25 27306.0 26 32061.0 27 37458.0 28 44359.0 29 52238.0 30 64034.0 31 77322.0 32 94708.0 33 119801.0 34 156556.0 35 209093.0 36 282981.0 37 337173.0 38 223475.0 39 28412.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.41645019779664 17.55980783929873 13.925481172961767 23.098260789942852 2 16.209255801155287 19.62845383801124 38.80440924032945 25.35788112050403 3 20.4867838336063 23.420902523275483 28.565525109124874 27.526788533993347 4 13.432557319483115 15.59540513604118 35.686352294126145 35.285685250349566 5 14.853791659666346 36.87294061980639 32.62641110555439 15.646856614972867 6 33.40962309071492 34.27856212095976 16.86534335289565 15.446471435429668 7 30.252968735538293 31.902160153298357 19.41934280135806 18.425528309805287 8 27.75490076534747 32.84579098066235 18.92605526034987 20.473252993640305 9 27.1563674254035 16.65914230061491 19.51760163035548 36.66688864362611 10-11 26.294891358998868 25.416907591779918 25.85166262206362 22.436538427157593 12-13 27.53502657957256 22.659970284559506 26.439223793707157 23.365779342160785 14-15 24.068295912391317 24.234540479450313 25.153513024368173 26.543650583790203 16-17 23.493498302222925 27.459009971196064 25.59706221395615 23.450429512624858 18-19 23.378048042908098 26.228026164263447 27.597896222009844 22.796029570818614 20-21 24.38737975733919 24.992844265508232 27.366809683416594 23.252966293735984 22-23 23.850572953836853 24.86633117356076 27.58252775407727 23.70056811852512 24-25 23.957224854216253 25.13781554858532 27.5578710248669 23.34708857233153 26-27 23.506204995575292 25.174056888407996 27.896005277826397 23.42373283819031 28-29 23.22992721278376 26.0029355764349 27.587319445648017 23.179817765133322 30-31 23.479379102495777 25.174026729428494 27.459318689179383 23.887275478896346 32-33 23.26686780536465 25.263355017961725 27.55555283562725 23.91422434104637 34-35 24.144236637269138 25.26978335814339 27.188615427473483 23.39736457711399 36-37 24.13181345709338 24.89496667477785 27.54311372583964 23.430106142289127 38-39 23.81233090128209 25.231585070058156 27.371984375596707 23.584099653063042 40-41 23.785473962097132 25.358068900417337 27.379896243985264 23.476560893500267 42-43 23.66056292965668 25.468893853824138 26.82061338628031 24.04992983023887 44-45 23.728942180069726 25.346861320587056 26.947081831316144 23.97711466802707 46-47 24.3406364749082 25.72276621787026 26.440832313341495 23.49576499388005 48-49 24.22515745070853 25.688773411980353 26.326874720936246 23.759194416374875 50-51 23.786764858879295 25.98943121612495 26.595445978738564 23.628357946257193 52-53 24.264153659207675 25.961667448370807 26.077478793623253 23.696700098798264 54-55 23.718961955453416 25.75140874221884 26.293877616965567 24.235751685362178 56-57 24.020710746484298 25.896217627830794 25.99778361426565 24.08528801141926 58-59 24.452143592234247 25.46912958884821 26.03103736292574 24.047689455991804 60-61 23.878458551239483 25.71092646138692 26.740817587461922 23.66979739991168 62-63 23.785986269496323 25.824656400816142 26.63864589669932 23.750711432988215 64-65 23.55436256725859 26.251634034927957 26.255330503454115 23.938672894359335 66-67 23.70810914022453 26.005331675242715 26.027338911437397 24.25922027309536 68-69 23.7272679646912 25.775629799107634 26.439567046621264 24.057535189579898 70-71 24.00821702704906 25.727833869359458 26.059193245801577 24.20475585778991 72-73 23.848086825713317 25.7588253510128 26.372221059421562 24.02086676385232 74-75 23.606353191503946 26.550635715723025 25.949387540079538 23.893623552693487 76-77 23.89008676613291 25.95853027316497 25.93877509055071 24.212607870151412 78-79 23.847951263064942 26.15084882646307 26.07043033687119 23.930769573600795 80-81 24.137687875000154 26.099067985744945 25.867833449432137 23.895410689822764 82-83 24.01104239601487 26.121969173433524 26.0269381782005 23.840050252351112 84-85 23.87396518193409 26.351236885449776 25.831477662422614 23.943320270193517 86-87 23.909254818726293 26.337302884197566 25.711599828145253 24.04184246893089 88-89 23.76517936157634 26.3603437534043 25.363576386721356 24.510900498298003 90-91 23.8612602136059 26.072821862472072 25.66629739572176 24.399620528200263 92-93 24.07107772390876 26.074138489482074 25.896299734686316 23.958484051922845 94-95 23.813090994194653 25.901194999870253 26.081544162900315 24.20416984303478 96-97 23.478326433251993 25.852514188577853 26.314499766950895 24.354659611219258 98-99 23.54049466822324 26.188141987448187 25.64600293032257 24.625360414006 100-101 23.579201283236497 25.924071092281604 25.88966370142711 24.60706392305479 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 191.0 1 143.5 2 168.5 3 241.5 4 214.5 5 220.5 6 356.0 7 636.5 8 1011.5 9 1301.0 10 1272.0 11 1126.0 12 1168.5 13 1283.0 14 1404.0 15 1530.5 16 1658.5 17 1811.0 18 2000.0 19 2209.5 20 2489.5 21 2862.5 22 3277.5 23 3913.0 24 4929.0 25 6085.0 26 7568.0 27 9567.0 28 11723.0 29 13995.0 30 16338.5 31 19557.0 32 23795.0 33 25811.5 34 26787.0 35 29297.0 36 32919.0 37 36544.5 38 39740.0 39 42440.5 40 45036.5 41 48691.5 42 51797.0 43 55125.0 44 58833.5 45 63517.0 46 68137.5 47 76123.0 48 91636.5 49 93270.5 50 82344.0 51 78115.0 52 78145.0 53 80913.0 54 83276.5 55 80636.5 56 75091.0 57 68966.5 58 64738.0 59 59583.5 60 51126.0 61 43972.5 62 37693.5 63 29762.5 64 21824.5 65 16602.5 66 12772.5 67 10282.0 68 8240.5 69 6305.5 70 4931.0 71 3680.5 72 2915.5 73 2476.5 74 1952.0 75 1452.0 76 1066.5 77 703.0 78 451.0 79 283.0 80 186.0 81 123.0 82 87.5 83 58.5 84 33.0 85 22.0 86 17.5 87 14.0 88 13.5 89 10.0 90 6.5 91 5.5 92 4.5 93 3.0 94 4.0 95 5.0 96 2.5 97 0.5 98 0.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015225990913363623 2 0.00597289675701081 3 0.008567679774400752 4 0.0350050916496945 5 0.04832171392762024 6 0.0597289675701081 7 0.02589887200375999 8 0.02354887983706721 9 0.014981200062666459 10-11 0.04653474071753094 12-13 0.04212850540498198 14-15 0.018995770014099952 16-17 0.02340200532664891 18-19 0.030084795550681498 20-21 0.024062940623531256 22-23 0.014320264765784114 24-25 0.0032067601441328527 26-27 0.0025703039323202253 28-29 0.001517703274322419 30-31 0.006340083033056556 32-33 0.0011015588281372397 34-35 0.002031764060786464 36-37 0.005532273225755915 38-39 0.005581231395895347 40-41 0.003720820930596898 42-43 6.609352968823438E-4 44-45 0.0014197869340435531 46-47 0.002937490208365972 48-49 7.833307222309259E-4 50-51 0.0017380150399498669 52-53 6.854143819520601E-4 54-55 0.0022765549114836282 56-57 0.0022275967413441958 58-59 0.002643741187529375 60-61 6.854143819520601E-4 62-63 0.0013463496788344038 64-65 0.0034270719097603004 66-67 0.0024479085069716434 68-69 0.006021854927150242 70-71 0.010550485665047783 72-73 0.0037942581858060476 74-75 0.002888532038226539 76-77 0.0028395738680871063 78-79 0.036278004073319756 80-81 0.023377526241579197 82-83 0.003353634654551152 84-85 0.008420805263982454 86-87 0.00673174839417202 88-89 0.005263003289989033 90-91 0.012117147109509634 92-93 0.012753603321322263 94-95 0.006511436628544571 96-97 0.0035249882500391666 98-99 0.02271659094469685 100-101 0.04149204919316936 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2042560.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.53709373657203 #Duplication Level Percentage of deduplicated Percentage of total 1 77.72596923572516 43.166744392103816 2 12.51197864935562 13.89757862158502 3 4.102781024978663 6.835696030946073 4 1.7745000138590288 3.94202294420949 5 0.9956207673161379 2.7646941940257057 6 0.6384813952595152 2.127564065855099 7 0.41200867633445937 1.6017235134507486 8 0.28813154098551697 1.280159072014048 9 0.21630698123059505 1.0811754983230635 >10 1.2076194033963685 12.204539975712553 >50 0.08003786935591276 3.076422649574039 >100 0.040812388338300754 4.1177255356716875 >500 0.0036282185915639587 1.3615550820780562 >1k 0.0019468490003513876 1.9411399464639358 >5k 1.7698627275921706E-4 0.6012584779866772 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6664 0.32625724580918064 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5570 0.27269700767664107 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4413 0.21605240482531723 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4392 0.21502428325238915 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2870 0.14050994830017233 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2693 0.13184435218549273 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2409 0.11794023186589378 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 4.895817013943287E-5 0.0 0.0 0.0 0.0 12-13 4.895817013943287E-5 0.0 0.0 0.0 4.895817013943287E-5 14-15 4.895817013943287E-5 4.895817013943287E-5 0.0 0.0 1.2239542534858217E-4 16-17 7.343725520914931E-5 9.791634027886574E-5 0.0 0.0 1.468745104182986E-4 18-19 9.791634027886574E-5 9.791634027886574E-5 0.0 4.895817013943287E-5 1.468745104182986E-4 20-21 9.791634027886574E-5 9.791634027886574E-5 0.0 4.895817013943287E-5 1.468745104182986E-4 22-23 9.791634027886574E-5 9.791634027886574E-5 0.0 9.791634027886574E-5 1.9583268055773148E-4 24-25 9.791634027886574E-5 1.468745104182986E-4 0.0 3.182281059063136E-4 2.4479085069716434E-4 26-27 9.791634027886574E-5 1.468745104182986E-4 0.0 6.364562118126273E-4 2.4479085069716434E-4 28-29 9.791634027886574E-5 1.7135359548801503E-4 0.0 0.0012729124236252546 2.4479085069716434E-4 30-31 1.468745104182986E-4 1.9583268055773148E-4 0.0 0.0026192621024596586 2.4479085069716434E-4 32-33 1.468745104182986E-4 1.9583268055773148E-4 0.0 0.00494477518408272 2.4479085069716434E-4 34-35 1.7135359548801503E-4 1.9583268055773148E-4 0.0 0.00734372552091493 2.4479085069716434E-4 36-37 1.9583268055773148E-4 1.9583268055773148E-4 0.0 0.010232257559141469 2.4479085069716434E-4 38-39 1.9583268055773148E-4 1.9583268055773148E-4 0.0 0.01339005953313489 2.4479085069716434E-4 40-41 1.9583268055773148E-4 2.937490208365972E-4 0.0 0.016523382422058593 2.4479085069716434E-4 42-43 1.9583268055773148E-4 2.937490208365972E-4 0.0 0.019167123609587968 2.692699357668808E-4 44-45 1.9583268055773148E-4 3.182281059063136E-4 0.0 0.022887944540184866 2.937490208365972E-4 46-47 1.9583268055773148E-4 3.4270719097603006E-4 0.0 0.02780824063919787 4.4062353125489583E-4 48-49 1.9583268055773148E-4 3.4270719097603006E-4 0.0 0.03400144916183613 4.4062353125489583E-4 50-51 1.9583268055773148E-4 3.9166536111546297E-4 0.0 0.038627996240012535 4.4062353125489583E-4 52-53 1.9583268055773148E-4 3.9166536111546297E-4 0.0 0.04815036033213223 4.895817013943287E-4 54-55 1.9583268055773148E-4 3.9166536111546297E-4 0.0 0.06452686824377252 4.895817013943287E-4 56-57 1.9583268055773148E-4 4.4062353125489583E-4 0.0 0.096080408898637 4.895817013943287E-4 58-59 1.9583268055773148E-4 4.4062353125489583E-4 0.0 0.13287247375842082 4.895817013943287E-4 60-61 1.9583268055773148E-4 4.4062353125489583E-4 0.0 0.16944422685257715 5.385398715337616E-4 62-63 1.9583268055773148E-4 4.4062353125489583E-4 0.0 0.2112055459815134 5.874980416731944E-4 64-65 1.9583268055773148E-4 4.4062353125489583E-4 0.0 0.26403141156196147 5.874980416731944E-4 66-67 1.9583268055773148E-4 4.4062353125489583E-4 0.0 0.34726030079899733 5.874980416731944E-4 68-69 1.9583268055773148E-4 4.4062353125489583E-4 0.0 0.45051308162306125 5.874980416731944E-4 70-71 1.9583268055773148E-4 4.4062353125489583E-4 0.0 0.5396903885320382 5.874980416731944E-4 72-73 1.9583268055773148E-4 4.4062353125489583E-4 0.0 0.6290880072066427 5.874980416731944E-4 74-75 1.9583268055773148E-4 4.4062353125489583E-4 0.0 0.7295256932476892 5.874980416731944E-4 76-77 1.9583268055773148E-4 4.4062353125489583E-4 0.0 0.8467315525614915 5.874980416731944E-4 78-79 1.9583268055773148E-4 4.895817013943287E-4 0.0 0.9423468588438039 5.874980416731944E-4 80-81 1.9583268055773148E-4 4.895817013943287E-4 0.0 1.0426131912893624 5.874980416731944E-4 82-83 1.9583268055773148E-4 4.895817013943287E-4 0.0 1.1569060394798685 6.364562118126273E-4 84-85 1.9583268055773148E-4 4.895817013943287E-4 0.0 1.3281372395425348 6.364562118126273E-4 86-87 1.9583268055773148E-4 4.895817013943287E-4 0.0 1.5620104182986057 7.098934670217766E-4 88-89 1.9583268055773148E-4 4.895817013943287E-4 0.0 1.837351167162776 8.078098073006424E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGCGT 115 3.8554754E-6 28.923653 8 GTACCGT 220 2.1609594E-9 23.759878 6 TACATGG 9875 0.0 22.71324 2 GTACATG 10065 0.0 22.709394 1 ACATGGG 10405 0.0 20.779903 3 TAAGGTA 580 0.0 20.482655 4 GAGTACT 6165 0.0 20.153925 12-13 GTACTTT 6275 0.0 19.686085 14-15 AGTACTT 6325 0.0 18.817772 12-13 GTAGCCC 935 0.0 18.80461 3 GTATCAA 17415 0.0 18.74595 1 GTGTAAG 745 0.0 18.497623 1 TCGCGTG 130 0.0075491 18.275934 9 CATGGGG 5285 0.0 18.072792 4 TACCTGG 1635 0.0 17.729082 2 GTACCTG 1755 0.0 17.329142 1 TAGACCG 165 0.0014602663 17.279911 4 TACTTTT 7010 0.0 16.978115 14-15 TAGCCCT 1065 0.0 16.955406 4 AGAGTAC 13320 0.0 16.855225 10-11 >>END_MODULE