FastQCFastQC Report
Thu 26 May 2016
SRR936502_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936502_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2042560
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT75450.369389393702021No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63850.3125979163402789No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56100.2746553344822184No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT35730.17492754190819365No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31360.15353282155726147No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT25940.12699749334168886No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC25490.12479437568541439No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT22080.10809963966786779No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGTG9600.023.2515475
GTACATG105500.022.788751
TACATGG104800.022.3916742
GTAAGGT8500.022.3527054
TACCGTA1553.9130813E-521.4482367
ACATGGG106050.021.2303283
GAGTACT80400.020.5844912-13
GTACTTT81750.019.95410714-15
AGTACTT82950.019.35056312-13
CATGGGG56350.018.1231444
GTATCAA199900.018.0526221
TACTTTT88400.017.91582914-15
ACTTTTT94600.017.5448416-17
AGAGTAC150100.017.46432310-11
GGTAAGG10200.017.2302113
CATGGGA49300.017.0535724
CCCTATA6150.016.9929392
GGGCGTA4204.7293724E-1116.9616159
GTATAGG4557.2759576E-1216.8750021
ACCGTGC4801.8189894E-1216.8202698
Produced by FastQC (version 0.11.5)