##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936502_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2042560 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.454618224189254 31.0 31.0 34.0 30.0 34.0 2 31.65601793827354 31.0 31.0 34.0 30.0 34.0 3 31.939622336675544 31.0 31.0 34.0 30.0 34.0 4 35.75233873178756 37.0 35.0 37.0 35.0 37.0 5 35.55192209775967 37.0 35.0 37.0 33.0 37.0 6 35.652599189252705 37.0 35.0 37.0 33.0 37.0 7 35.5863044414852 37.0 35.0 37.0 33.0 37.0 8 35.56257686432712 37.0 35.0 37.0 33.0 37.0 9 37.18404649067836 39.0 37.0 39.0 33.0 39.0 10-11 37.071835833463886 39.0 37.0 39.0 33.0 39.0 12-13 36.935580839730534 39.0 37.0 39.0 33.0 39.0 14-15 38.04436883127056 40.0 37.0 41.0 33.0 41.0 16-17 37.95047342550525 40.0 37.0 41.0 33.0 41.0 18-19 37.99909965925114 40.0 37.0 41.0 33.0 41.0 20-21 38.06397951590161 40.0 37.0 41.0 33.0 41.0 22-23 37.97552434200219 40.0 37.0 41.0 33.0 41.0 24-25 37.8556184396052 40.0 37.0 41.0 33.0 41.0 26-27 37.631699680792735 39.0 37.0 41.0 32.0 41.0 28-29 37.55510364444619 39.0 36.5 41.0 32.0 41.0 30-31 37.56038035602381 39.0 37.0 41.0 32.0 41.0 32-33 37.3897988308789 39.0 36.0 41.0 32.0 41.0 34-35 37.217353712987624 39.0 36.0 41.0 31.0 41.0 36-37 36.97511749960833 39.0 35.5 41.0 31.0 41.0 38-39 36.809962742832525 39.0 35.0 41.0 31.0 41.0 40-41 36.57318120397932 39.0 35.0 40.5 30.0 41.0 42-43 36.3204713692621 39.0 35.0 40.0 30.0 41.0 44-45 36.035294924016924 38.5 35.0 40.0 29.5 41.0 46-47 35.638808896678675 38.0 35.0 40.0 28.5 41.0 48-49 35.17830908271972 38.0 34.0 40.0 27.0 41.0 50-51 34.95752389158703 38.0 34.0 40.0 26.0 41.0 52-53 34.65616505757481 38.0 34.0 40.0 26.0 41.0 54-55 34.356617431066894 38.0 33.0 40.0 25.0 41.0 56-57 34.0346937176876 37.0 33.0 40.0 24.0 41.0 58-59 33.63149234294219 36.5 32.5 39.5 23.0 41.0 60-61 33.22235944109353 36.0 32.0 39.0 22.0 40.5 62-63 32.901799457543476 36.0 32.0 39.0 22.0 40.0 64-65 32.4315672489425 35.0 31.0 38.5 21.5 40.0 66-67 31.992898127839574 35.0 31.0 38.0 20.0 40.0 68-69 31.52572996631678 35.0 30.5 37.0 18.5 39.5 70-71 31.0404130111233 34.0 30.0 37.0 18.0 39.0 72-73 30.61851573515588 34.0 30.0 36.0 16.5 39.0 74-75 30.262210902005325 34.0 29.0 36.0 13.5 38.5 76-77 28.867585774714087 32.5 28.0 34.5 11.0 36.0 78-79 29.673002506658314 34.0 29.0 35.0 9.5 37.0 80-81 29.541827657449474 34.0 29.0 35.0 7.0 36.5 82-83 29.21956735665048 34.0 29.0 35.0 6.5 36.0 84-85 28.780672538383207 33.5 29.0 35.0 2.0 36.0 86-87 28.41664357472975 33.0 28.0 35.0 2.0 35.0 88-89 28.00030133753721 33.0 27.0 35.0 2.0 35.0 90-91 27.53812470625098 33.0 26.5 35.0 2.0 35.0 92-93 27.070097328842238 33.0 25.5 34.5 2.0 35.0 94-95 26.50027343138023 32.0 25.0 34.0 2.0 35.0 96-97 25.782207377017077 32.0 23.5 34.0 2.0 35.0 98-99 24.885833219097606 32.0 19.0 34.0 2.0 35.0 100-101 22.04504861546295 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4526773816898668 1101 2 0.39197681151940245 1101 3 0.284215642102879 1101 4 0.12100594005421073 1101 5 0.2892991483899152 1101 6 0.2733222945864071 1101 7 0.31340281388195734 1101 8 0.302860045026776 1101 9 0.4240331475177683 1101 10-11 0.4333643070352551 1101 12-13 0.444972117076297 1101 14-15 0.5059908279505763 1101 16-17 0.4442728999951058 1101 18-19 0.5369800887549943 1101 20-21 0.3640607844543595 1101 22-23 0.26065594959353433 1101 24-25 0.2703027927142756 1101 26-27 0.3380292427127145 1101 28-29 0.4060259266005062 1101 30-31 0.23835955450793023 1101 32-33 0.31747999356264955 1101 34-35 0.4586436992223355 1101 36-37 0.41717366875956685 1101 38-39 0.38750299554613576 1101 40-41 0.5500401839422153 1101 42-43 0.4685292181863616 1101 44-45 0.3314616637504386 1101 46-47 0.5177575773850833 1101 48-49 0.49689910950649363 1101 50-51 0.3269102519056517 1101 52-53 0.30052584402564264 1101 54-55 0.22774294967358344 1101 56-57 0.12223187422198833 1101 58-59 0.3119800352610014 1101 60-61 0.3879616435245836 1101 62-63 0.11934987958807142 1101 64-65 0.014128590047967293 1101 66-67 -0.0034502890732923674 1101 68-69 -0.1466886921973405 1101 70-71 -0.15049787314978857 1101 72-73 -0.11174421553905489 1101 74-75 -0.2048588743581412 1101 76-77 0.273237369167564 1101 78-79 0.18650176280520725 1101 80-81 0.020802281639898723 1101 82-83 0.19982122423281368 1101 84-85 0.2937429395550559 1101 86-87 0.19881098881305448 1101 88-89 0.03556299220420556 1101 90-91 0.006942633140251786 1101 92-93 0.06528934482209792 1101 94-95 -0.21656015094040626 1101 96-97 -0.08754921192464593 1101 98-99 -0.18082889740852792 1101 100-101 -0.13948335744753848 1102 1 0.44277253868632016 1102 2 0.5419106952998298 1102 3 0.3290701368231197 1102 4 0.2150557855032602 1102 5 0.30674192714818105 1102 6 0.2648710164793897 1102 7 0.22254852212668652 1102 8 0.08170911157868233 1102 9 0.22742189944345625 1102 10-11 0.25072673913545884 1102 12-13 0.36983234975350854 1102 14-15 0.4318586367148427 1102 16-17 0.5076521446144326 1102 18-19 0.4529461066204945 1102 20-21 0.39451506735852604 1102 22-23 0.29444885360255313 1102 24-25 0.31931120202594343 1102 26-27 0.17325411797700951 1102 28-29 0.15798823921081606 1102 30-31 0.25682173625924776 1102 32-33 0.5567642444731362 1102 34-35 0.5352274936142436 1102 36-37 0.5479700926084092 1102 38-39 0.29138992727283863 1102 40-41 0.5147822696437885 1102 42-43 0.3356302756643572 1102 44-45 0.2742041782727327 1102 46-47 0.34939355895342317 1102 48-49 0.4447912878013014 1102 50-51 0.376811401862426 1102 52-53 0.19959960653068975 1102 54-55 0.26429724547289624 1102 56-57 0.327876207875029 1102 58-59 0.1893746975875743 1102 60-61 0.27486094161680086 1102 62-63 0.12215781597610231 1102 64-65 0.05628960230904312 1102 66-67 0.18792163965090225 1102 68-69 0.27809828996539565 1102 70-71 0.1090660879022991 1102 72-73 0.0418872931697436 1102 74-75 -0.2849701388375152 1102 76-77 0.17745736182725125 1102 78-79 0.43377031979296277 1102 80-81 0.5140239384597116 1102 82-83 0.49115034718761663 1102 84-85 0.46792922934782766 1102 86-87 0.15221412378247123 1102 88-89 0.08780586954545555 1102 90-91 0.24634896824151653 1102 92-93 0.45199826787930064 1102 94-95 0.284850302976249 1102 96-97 0.10466204561119241 1102 98-99 -0.2781605185110898 1102 100-101 -0.2421073282205839 1103 1 0.5579053229685691 1103 2 0.5786456928473278 1103 3 0.40718123242431403 1103 4 0.24193786553831131 1103 5 0.20134258554418238 1103 6 0.22669771641482583 1103 7 0.2391848475170022 1103 8 0.33079208769922985 1103 9 0.26313130368813376 1103 10-11 0.27936180337560046 1103 12-13 0.2907878721566135 1103 14-15 0.352228659727686 1103 16-17 0.3477222349015463 1103 18-19 0.4101039578377268 1103 20-21 0.4378925506417488 1103 22-23 0.23458154094781491 1103 24-25 0.1577451171750539 1103 26-27 0.2969831529305438 1103 28-29 0.5207640006992946 1103 30-31 0.5371415653011766 1103 32-33 0.4126954066118742 1103 34-35 0.36706981494484126 1103 36-37 0.42111859619217284 1103 38-39 0.6015087914040222 1103 40-41 0.6302122869567341 1103 42-43 0.5603222099255731 1103 44-45 0.6419063985174418 1103 46-47 0.7305500268798824 1103 48-49 0.6475020336538506 1103 50-51 0.3062360663150514 1103 52-53 0.4094223766407552 1103 54-55 0.513059844857537 1103 56-57 0.3694466645839398 1103 58-59 0.5286053135833342 1103 60-61 0.6605192843045486 1103 62-63 0.4742729325234407 1103 64-65 0.3600760578857276 1103 66-67 0.27872784516893745 1103 68-69 0.2155195286649665 1103 70-71 0.3677277710385596 1103 72-73 0.41411332016863156 1103 74-75 0.5003283737613842 1103 76-77 0.40644651263987086 1103 78-79 0.5632749814925795 1103 80-81 0.6998909137737392 1103 82-83 0.6922636926962369 1103 84-85 0.710378322890076 1103 86-87 0.5506173777631851 1103 88-89 0.6815441765216228 1103 90-91 0.45729511683816426 1103 92-93 0.5889255157025772 1103 94-95 0.7218324171331183 1103 96-97 0.4510268601998213 1103 98-99 0.2562209702083571 1103 100-101 0.13610504229583853 1104 1 0.3153225222281968 1104 2 0.3943451917215519 1104 3 0.12845413268507855 1104 4 0.11513097515169335 1104 5 -0.12190532890463857 1104 6 0.1254664158988632 1104 7 0.08484293211118654 1104 8 0.027268813726983865 1104 9 -0.0019502266186677275 1104 10-11 -0.027336859052581985 1104 12-13 -0.0588208435741393 1104 14-15 0.10679735216405817 1104 16-17 0.06708925296209856 1104 18-19 0.17270744717165343 1104 20-21 -0.007916270432986039 1104 22-23 0.036983680469241165 1104 24-25 -0.019129375115170433 1104 26-27 -0.033369220832867086 1104 28-29 0.0072848858854897 1104 30-31 0.06639468945498805 1104 32-33 0.09553096799514549 1104 34-35 0.1302487086846611 1104 36-37 0.17768636686871275 1104 38-39 0.2715994465768219 1104 40-41 0.20014339607306653 1104 42-43 0.1322097403881628 1104 44-45 0.19334110750469335 1104 46-47 0.11604547742941662 1104 48-49 0.08663064604363768 1104 50-51 -0.0775218113004641 1104 52-53 0.028493995710981324 1104 54-55 0.13488329724688697 1104 56-57 0.19615859701541893 1104 58-59 0.16002401696213298 1104 60-61 0.13257415034335196 1104 62-63 -0.046211186129490045 1104 64-65 -0.16500248643849602 1104 66-67 0.0889204317717116 1104 68-69 -0.026518701185214155 1104 70-71 -0.24562481044537776 1104 72-73 -0.2051263763368425 1104 74-75 -0.15171679247067615 1104 76-77 0.10622775696001341 1104 78-79 0.058882712405473825 1104 80-81 0.02300204796491201 1104 82-83 -0.01168517652514467 1104 84-85 0.061942532643296744 1104 86-87 0.040408470261880325 1104 88-89 -0.07269137484655275 1104 90-91 -0.2665429928308569 1104 92-93 -0.18059117241617884 1104 94-95 -0.22960850492346196 1104 96-97 -0.3056512620066165 1104 98-99 -0.0220357445431425 1104 100-101 0.05064496914853933 1105 1 0.43730980152745147 1105 2 0.42257413198960947 1105 3 0.2110412022787571 1105 4 0.1311630293768431 1105 5 -0.03454777191171132 1105 6 -0.00268264936592999 1105 7 0.1012487546305465 1105 8 0.04785751675998284 1105 9 0.072707743945287 1105 10-11 0.07187616102218897 1105 12-13 0.18561283905884096 1105 14-15 0.14258386937132883 1105 16-17 0.1336083290715706 1105 18-19 0.12071142564763448 1105 20-21 0.1338077268392226 1105 22-23 0.10756771139856625 1105 24-25 0.11617991365587699 1105 26-27 0.06809719078485443 1105 28-29 0.10514726368077021 1105 30-31 0.020048768695666297 1105 32-33 0.20130312746842094 1105 34-35 0.22425592708484743 1105 36-37 0.28583995149533337 1105 38-39 0.1996542162106678 1105 40-41 0.3099531066811636 1105 42-43 0.3223405503063077 1105 44-45 -0.03387048218756661 1105 46-47 0.15760428888217604 1105 48-49 0.005601543330136849 1105 50-51 0.01874688497596111 1105 52-53 0.0481684938416862 1105 54-55 0.16052461086273695 1105 56-57 0.0959417485110805 1105 58-59 0.11813546280751552 1105 60-61 0.2126319945808106 1105 62-63 0.11839354042216854 1105 64-65 0.08905393280733875 1105 66-67 0.03075588567107701 1105 68-69 -0.11104289684213242 1105 70-71 0.0337133052053602 1105 72-73 -0.05790187546576675 1105 74-75 -0.1427238913728246 1105 76-77 -0.011518240529628798 1105 78-79 0.23145427420886833 1105 80-81 0.03308458247333945 1105 82-83 0.23038594906549648 1105 84-85 0.3713719160424169 1105 86-87 0.17108316695699344 1105 88-89 0.04159883826426736 1105 90-91 -0.16955528675289955 1105 92-93 -0.01696556605348931 1105 94-95 0.1153389743418316 1105 96-97 0.09838664852080115 1105 98-99 -0.07363014484718633 1105 100-101 0.016928553263028334 1106 1 0.35214458883148936 1106 2 0.3870308338865094 1106 3 0.07240517305706362 1106 4 0.03233875502554184 1106 5 -0.0668177027547543 1106 6 0.05673241298402587 1106 7 -0.006392799282139094 1106 8 0.045145102488142186 1106 9 0.06477597032196059 1106 10-11 -0.06467568150621616 1106 12-13 0.11695055838615787 1106 14-15 0.07260967895308568 1106 16-17 0.1541804348391551 1106 18-19 -0.0496660588173512 1106 20-21 0.026509836720613578 1106 22-23 0.1028294371718701 1106 24-25 -0.008914480658503976 1106 26-27 -0.05020953981144771 1106 28-29 -0.0335859767716471 1106 30-31 -0.03270500453616165 1106 32-33 0.011576410331450404 1106 34-35 -0.32072183142743427 1106 36-37 -0.17455486749956606 1106 38-39 -0.18464122109184444 1106 40-41 -0.1421841706001743 1106 42-43 -0.16260165098898227 1106 44-45 -0.042926371133461316 1106 46-47 -0.1947536137059771 1106 48-49 -0.24652749309004918 1106 50-51 -0.1624593782552637 1106 52-53 -0.0018188466048556506 1106 54-55 -0.16298784741928074 1106 56-57 -0.35212248867019014 1106 58-59 -0.390375711844392 1106 60-61 -0.12260446952858928 1106 62-63 -0.37529203780569986 1106 64-65 -0.23488117339372394 1106 66-67 -0.23294393842078165 1106 68-69 -0.36056722461542634 1106 70-71 -0.2884256025734402 1106 72-73 0.00691581149904863 1106 74-75 -0.1886385231212131 1106 76-77 -0.20051929792269618 1106 78-79 0.018942680664931544 1106 80-81 -0.03985289347240695 1106 82-83 -0.12737468860482792 1106 84-85 -0.12991712697046154 1106 86-87 -0.2868651325755529 1106 88-89 -0.27030361645186574 1106 90-91 -0.5395196897676833 1106 92-93 -0.3293356288828768 1106 94-95 -0.1862277172020761 1106 96-97 -0.23987153832183594 1106 98-99 -0.2685570000241029 1106 100-101 -0.11961831823654023 1107 1 0.47518596795164214 1107 2 0.47789767191615695 1107 3 0.25735309373558835 1107 4 0.14816591425142178 1107 5 0.03113378687645252 1107 6 0.17079839438895306 1107 7 0.06252089064215482 1107 8 0.16273106213510857 1107 9 0.18112425559853307 1107 10-11 0.27855429199053106 1107 12-13 0.24918451069932246 1107 14-15 0.430996289817422 1107 16-17 0.3918581335921587 1107 18-19 0.3455074866180894 1107 20-21 0.25488738493666574 1107 22-23 0.2521376375847808 1107 24-25 0.20853891517191414 1107 26-27 0.1535544309788648 1107 28-29 0.2614122591976482 1107 30-31 0.2581120982882865 1107 32-33 0.35099255896268033 1107 34-35 0.22873379155118556 1107 36-37 0.4056224675112645 1107 38-39 0.47108263060024314 1107 40-41 0.6089491359160917 1107 42-43 0.5250077688398704 1107 44-45 0.277139644506029 1107 46-47 0.47875544751587285 1107 48-49 0.5475610308204111 1107 50-51 0.5107229093549606 1107 52-53 0.20956207561707885 1107 54-55 0.40191825955039917 1107 56-57 0.42452614304109204 1107 58-59 0.2200693706941692 1107 60-61 0.25978793648567233 1107 62-63 0.3314997894543268 1107 64-65 0.3929003844498382 1107 66-67 0.34486978564297743 1107 68-69 0.330424403133204 1107 70-71 0.4501960427964775 1107 72-73 0.5385198954916355 1107 74-75 0.5408869526202729 1107 76-77 0.36649615707835537 1107 78-79 0.5657749617610826 1107 80-81 0.4205935917320822 1107 82-83 0.5144084468812871 1107 84-85 0.6347376412853549 1107 86-87 0.8880091505195189 1107 88-89 0.9268648527593797 1107 90-91 0.7776423319351231 1107 92-93 0.8639721806854936 1107 94-95 0.9422919044299825 1107 96-97 0.9406421749960714 1107 98-99 1.2113757243144825 1107 100-101 0.833159031609263 1108 1 0.2565775042543983 1108 2 0.351893892983707 1108 3 0.07583929785351273 1108 4 0.05485267965195817 1108 5 -0.01487332640816419 1108 6 -0.07339427559354306 1108 7 0.04997539062826206 1108 8 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-1.4112064867283358 2316 74-75 -1.6012727314325197 2316 76-77 -1.7804121558188974 2316 78-79 -1.3360657329773495 2316 80-81 -1.2400754654408566 2316 82-83 -1.240094360197201 2316 84-85 -1.3500854406424772 2316 86-87 -1.504040870529888 2316 88-89 -1.560026861035393 2316 90-91 -1.6024675469596303 2316 92-93 -1.7562366255235453 2316 94-95 -1.9491463835121898 2316 96-97 -1.7944418165606137 2316 98-99 -2.3477496850204673 2316 100-101 -3.178633911883132 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 15.0 4 23.0 5 78.0 6 171.0 7 417.0 8 1031.0 9 1847.0 10 2869.0 11 4669.0 12 6194.0 13 8418.0 14 10683.0 15 13713.0 16 17220.0 17 20935.0 18 18514.0 19 13922.0 20 13219.0 21 13964.0 22 14880.0 23 16906.0 24 19723.0 25 24059.0 26 29292.0 27 36564.0 28 45271.0 29 55572.0 30 67576.0 31 82706.0 32 102745.0 33 128873.0 34 168903.0 35 225720.0 36 308913.0 37 367200.0 38 190253.0 39 9498.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.82419108443423 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23.175451394328686 26.045134537051545 28.172024322418928 22.607389746200845 20-21 24.233241618361273 24.6642203900987 28.04534505718314 23.057192934356884 22-23 23.651761169331248 24.649442971601466 28.23721502936302 23.461580829704264 24-25 23.8313195206016 24.916722152592826 28.237554833150558 23.014403493655024 26-27 23.416483930207157 24.94561968589402 28.50492935577381 23.132967028125016 28-29 23.034672176092748 25.691142487858375 28.272780236565875 23.001405099483 30-31 23.293299411840877 24.976946815992385 28.069219036214122 23.660534735952613 32-33 23.17057616951545 25.05145091960486 28.196890986229732 23.581081924649954 34-35 23.989926312661286 25.01195809745568 27.85419101328781 23.143924576595225 36-37 24.00556866857707 24.747282618008626 28.101360147544746 23.145788565869555 38-39 23.54154763705724 24.923049958410015 28.160337106541355 23.375065297991394 40-41 23.6105283514934 25.13421151195725 27.921502483494372 23.333757653054978 42-43 23.554148451196415 25.24794542715701 27.386405116301958 23.811501005344617 44-45 23.693288610647766 25.027894559300236 27.495026959436736 23.783789870615266 46-47 24.321547567495188 25.555994280635165 26.874184989286594 23.248273162583054 48-49 24.30240543097866 25.54773099224793 26.64597162837554 23.503891948397868 50-51 23.882555715241452 25.81748856760772 26.875283193593898 23.424672523556932 52-53 24.306364232553324 25.86140674995771 26.393079321513436 23.439149695975527 54-55 23.801267618012623 25.63052232628886 26.576743427680434 23.991466628018085 56-57 24.121796255850082 25.803026007922668 26.206234244132048 23.868943492095198 58-59 24.51694901147494 25.33267434926547 26.314247274714102 23.83612936454548 60-61 23.88122145111275 25.60066038675809 27.00150971424123 23.51660844788793 62-63 23.82874053576233 25.669090911761444 26.877223234466335 23.62494531800989 64-65 23.73629586031479 25.983898408619442 26.580283568791913 23.69952216227386 66-67 23.746534880327914 25.85204489796318 26.34891196955884 24.052508252150062 68-69 23.87082070924354 25.57985544096332 26.622739689208107 23.926584160585033 70-71 24.04919947790547 25.612374188633574 26.354268357762045 23.98415797569891 72-73 23.97085030549898 25.64081348895504 26.555792730690897 23.832543474855083 74-75 23.69298821087263 26.383606846310514 26.086063567288107 23.837341375528748 76-77 23.97876865961191 25.7718000544908 26.1376423988389 24.111788887058385 78-79 23.94516707351019 25.923281005528192 26.32695824098667 23.80459367997495 80-81 24.167731668502874 25.92141020932795 26.043261109070876 23.867597013098298 82-83 24.057768061087838 25.88376858500567 26.22047147708104 23.83799187682545 84-85 23.856803655114316 26.151115955089075 25.99769339822762 23.99438699156898 86-87 23.991113232836973 26.13279963767451 25.776055915636913 24.100031213851604 88-89 23.84814040606927 26.109903004003687 25.564283690700968 24.477672899226075 90-91 23.88280429570021 25.860950688474077 25.840554902493206 24.415690113332502 92-93 24.11542070407961 25.90295129449763 25.991714608076677 23.98991339334608 94-95 23.803809244026635 25.66607912260086 26.186006658832746 24.344104974539757 96-97 23.517277578171896 25.581411290283096 26.441005935296356 24.46030519624865 98-99 23.561927485377826 25.945639861327123 25.730961796411556 24.761470856883495 100-101 23.32728218624721 25.767751017535268 25.945760014689228 24.95920678152829 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 341.0 1 244.5 2 214.0 3 284.5 4 245.5 5 228.0 6 384.5 7 735.5 8 1154.0 9 1407.0 10 1290.0 11 1159.0 12 1251.0 13 1381.0 14 1560.0 15 1727.0 16 1950.5 17 2171.5 18 2385.0 19 2663.5 20 2936.5 21 3307.0 22 3839.0 23 4576.0 24 5570.5 25 6700.0 26 8242.5 27 10234.5 28 12338.5 29 14536.5 30 16957.5 31 20245.5 32 24519.5 33 26477.0 34 27156.5 35 29311.5 36 32619.0 37 36218.0 38 39689.0 39 42245.0 40 44763.5 41 48972.0 42 51852.0 43 54598.5 44 58263.0 45 62377.5 46 66737.5 47 75745.0 48 92371.0 49 93795.5 50 81779.0 51 77061.0 52 77555.0 53 80525.0 54 83033.0 55 80577.0 56 74635.5 57 68646.0 58 64687.5 59 59197.0 60 50545.0 61 43723.5 62 37401.0 63 29117.5 64 21175.0 65 16224.0 66 12615.5 67 10022.5 68 7885.0 69 6139.0 70 4832.5 71 3577.0 72 2890.5 73 2432.5 74 1880.0 75 1462.5 76 1078.0 77 682.5 78 455.0 79 313.0 80 222.0 81 145.0 82 98.0 83 65.5 84 35.0 85 20.5 86 10.5 87 3.5 88 4.5 89 4.0 90 1.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0036914460285131 2 0.010819755600814664 3 0.004602067993106689 4 0.0141489111702961 5 0.0 6 0.0 7 0.0 8 0.0 9 0.004014569951433495 10-11 2.937490208365972E-4 12-13 2.4479085069716434E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0012239542534858217 24-25 0.0 26-27 4.895817013943287E-5 28-29 0.0 30-31 2.4479085069716436E-5 32-33 0.003892174526084913 34-35 5.385398715337615E-4 36-37 0.002986448378505405 38-39 4.895817013943287E-5 40-41 0.003671862760457465 42-43 0.011040067366442112 44-45 0.0023010339965533447 46-47 0.0012484333385555382 48-49 0.009914029453235156 50-51 4.161444461851794E-4 52-53 0.00212968040106533 54-55 0.011040067366442112 56-57 0.003280197399342002 58-59 0.001077079743067523 60-61 0.00589945950180166 62-63 0.003353634654551152 64-65 0.016621298762337457 66-67 0.003035406548644838 68-69 1.7135359548801503E-4 70-71 8.812470625097917E-4 72-73 0.0 74-75 0.0 76-77 6.609352968823438E-4 78-79 0.010305694814350619 80-81 0.01517703274322419 82-83 0.012484333385555383 84-85 0.007906744477518409 86-87 0.009669238602537992 88-89 0.027832719724267582 90-91 0.023083777220742598 92-93 0.002423429421901927 94-95 0.007833307222309259 96-97 0.00988955036816544 98-99 0.009767154942816858 100-101 0.012117147109509634 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2042560.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.28255993952871 #Duplication Level Percentage of deduplicated Percentage of total 1 76.10970257360515 40.55319789357819 2 13.262148485988046 14.132824432631757 3 4.468298800743916 7.142471960350857 4 1.932821776473713 4.119427686295478 5 1.1024157746728231 2.9369767296143334 6 0.675565686962293 2.1597521525194354 7 0.44279641057693275 1.6515328401301508 8 0.316457019946254 1.3489312106856726 9 0.24159504059904305 1.1585522008630242 >10 1.303888753958216 12.687095196087805 >50 0.09233415112607814 3.3591852606326666 >100 0.04559622673905087 4.412465943568406 >500 0.004252865739148571 1.5923827214326662 >1k 0.0018490720604993763 1.782631200014968 >5k 2.7736080907490645E-4 0.9625725715945636 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7545 0.369389393702021 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6385 0.3125979163402789 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5610 0.2746553344822184 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3573 0.17492754190819365 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3136 0.15353282155726147 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2594 0.12699749334168886 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2549 0.12479437568541439 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2208 0.10809963966786779 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 4.895817013943287E-5 0.0 0.0 0.0 4 0.0 4.895817013943287E-5 0.0 0.0 0.0 5 0.0 9.791634027886574E-5 0.0 0.0 0.0 6 0.0 9.791634027886574E-5 0.0 0.0 0.0 7 0.0 9.791634027886574E-5 0.0 0.0 0.0 8 0.0 9.791634027886574E-5 0.0 0.0 0.0 9 0.0 9.791634027886574E-5 0.0 0.0 0.0 10-11 0.0 9.791634027886574E-5 0.0 0.0 0.0 12-13 0.0 9.791634027886574E-5 0.0 0.0 1.468745104182986E-4 14-15 0.0 1.468745104182986E-4 0.0 0.0 1.468745104182986E-4 16-17 0.0 1.468745104182986E-4 0.0 0.0 1.468745104182986E-4 18-19 0.0 1.9583268055773148E-4 0.0 0.0 1.9583268055773148E-4 20-21 0.0 2.4479085069716434E-4 0.0 0.0 1.9583268055773148E-4 22-23 0.0 2.4479085069716434E-4 0.0 4.895817013943287E-5 1.9583268055773148E-4 24-25 0.0 2.4479085069716434E-4 0.0 2.692699357668808E-4 1.9583268055773148E-4 26-27 0.0 2.4479085069716434E-4 0.0 5.385398715337616E-4 1.9583268055773148E-4 28-29 0.0 2.937490208365972E-4 0.0 0.0012239542534858217 1.9583268055773148E-4 30-31 0.0 2.937490208365972E-4 0.0 0.0026682202725990915 1.9583268055773148E-4 32-33 0.0 2.937490208365972E-4 0.0 0.005042691524361586 1.9583268055773148E-4 34-35 0.0 2.937490208365972E-4 0.0 0.0075395582014726615 1.9583268055773148E-4 36-37 0.0 2.937490208365972E-4 0.0 0.010477048409838634 1.9583268055773148E-4 38-39 0.0 2.937490208365972E-4 0.0 0.013879641234529217 1.9583268055773148E-4 40-41 0.0 2.937490208365972E-4 0.0 0.017110880463731788 1.9583268055773148E-4 42-43 0.0 2.937490208365972E-4 0.0 0.019950454331818895 1.9583268055773148E-4 44-45 0.0 2.937490208365972E-4 0.0 0.023646796177346073 1.9583268055773148E-4 46-47 0.0 2.937490208365972E-4 0.0 0.02849365502114993 1.9583268055773148E-4 48-49 0.0 2.937490208365972E-4 0.0 0.034515509948300174 1.9583268055773148E-4 50-51 0.0 2.937490208365972E-4 0.0 0.038872787090709696 1.9583268055773148E-4 52-53 0.0 2.937490208365972E-4 0.0 0.04827275575748081 2.4479085069716434E-4 54-55 0.0 2.937490208365972E-4 0.0 0.06469822183926054 2.4479085069716434E-4 56-57 0.0 3.9166536111546297E-4 0.0 0.0959335343882187 2.937490208365972E-4 58-59 0.0 3.9166536111546297E-4 0.0 0.1322849757167476 3.182281059063136E-4 60-61 0.0 3.9166536111546297E-4 0.0 0.16836714710950962 3.4270719097603006E-4 62-63 0.0 3.9166536111546297E-4 0.0 0.21020190349365503 4.4062353125489583E-4 64-65 0.0 3.9166536111546297E-4 0.0 0.2630767272442425 4.895817013943287E-4 66-67 0.0 4.4062353125489583E-4 0.0 0.3460363465455115 4.895817013943287E-4 68-69 4.895817013943287E-5 4.4062353125489583E-4 0.0 0.4485302757324142 4.895817013943287E-4 70-71 4.895817013943287E-5 4.4062353125489583E-4 0.0 0.5377320617264609 5.140607864640451E-4 72-73 4.895817013943287E-5 4.4062353125489583E-4 0.0 0.6275458248472505 6.364562118126273E-4 74-75 4.895817013943287E-5 4.4062353125489583E-4 0.0 0.7283506971643428 7.098934670217766E-4 76-77 4.895817013943287E-5 4.4062353125489583E-4 0.0 0.8456299937333542 7.833307222309259E-4 78-79 4.895817013943287E-5 4.895817013943287E-4 0.0 0.941122904590318 8.078098073006424E-4 80-81 4.895817013943287E-5 4.895817013943287E-4 0.0 1.041609548801504 8.322888923703587E-4 82-83 4.895817013943287E-5 4.895817013943287E-4 0.0 1.154947712674291 8.812470625097917E-4 84-85 4.895817013943287E-5 5.874980416731944E-4 0.0 1.325444540184866 8.812470625097917E-4 86-87 4.895817013943287E-5 5.874980416731944E-4 0.0 1.558632304558985 9.302052326492245E-4 88-89 4.895817013943287E-5 5.874980416731944E-4 0.0 1.832847015509948 0.0010036424878583738 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGGTG 960 0.0 23.251547 5 GTACATG 10550 0.0 22.78875 1 TACATGG 10480 0.0 22.391674 2 GTAAGGT 850 0.0 22.352705 4 TACCGTA 155 3.9130813E-5 21.448236 7 ACATGGG 10605 0.0 21.230328 3 GAGTACT 8040 0.0 20.58449 12-13 GTACTTT 8175 0.0 19.954107 14-15 AGTACTT 8295 0.0 19.350563 12-13 CATGGGG 5635 0.0 18.123144 4 GTATCAA 19990 0.0 18.052622 1 TACTTTT 8840 0.0 17.915829 14-15 ACTTTTT 9460 0.0 17.54484 16-17 AGAGTAC 15010 0.0 17.464323 10-11 GGTAAGG 1020 0.0 17.230211 3 CATGGGA 4930 0.0 17.053572 4 CCCTATA 615 0.0 16.992939 2 GGGCGTA 420 4.7293724E-11 16.961615 9 GTATAGG 455 7.2759576E-12 16.875002 1 ACCGTGC 480 1.8189894E-12 16.820269 8 >>END_MODULE