FastQCFastQC Report
Thu 26 May 2016
SRR936501_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936501_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2031651
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT65260.3212165869039515No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53680.2642186084125669No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43000.2116505246225853No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT42040.20692530360775546No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT28530.14042766203447343No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC27270.1342258094525093No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23810.11719532537822687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG104350.023.770542
GTACATG106750.023.5020471
ACATGGG107350.022.1766873
GAGTACT59550.021.63048212-13
GTACTTT63050.020.39204814-15
AGTACTT62500.019.81112-13
GTATCAA177350.019.0224651
TACCTGG17650.018.5765652
CATGGGA50300.018.047294
GTATATA9000.017.9504781
AGAGTAC131800.017.92138310-11
ACTTTTT73950.017.64233816-17
CATGGGG56850.017.3891944
TACTTTT69100.017.36848814-15
GTATAGG5050.016.9363861
GTACCGT1700.001781247116.7744736
TCAACGC199150.016.6579554
CAACGCA199300.016.621985
TATAGGG5450.016.5660172
ATCAACG200800.016.5414853
Produced by FastQC (version 0.11.5)