##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936501_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2031651 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.16390610395191 31.0 31.0 34.0 28.0 34.0 2 31.17699791942612 31.0 31.0 34.0 28.0 34.0 3 31.236885665894388 31.0 31.0 34.0 27.0 34.0 4 34.85790128324205 37.0 35.0 37.0 32.0 37.0 5 34.82006949028155 37.0 35.0 37.0 32.0 37.0 6 34.9538651077375 37.0 35.0 37.0 32.0 37.0 7 34.805789478606314 37.0 35.0 37.0 32.0 37.0 8 34.7462723666614 37.0 35.0 37.0 32.0 37.0 9 36.18011558087486 39.0 35.0 39.0 31.0 39.0 10-11 36.08721133698652 39.0 35.0 39.0 30.5 39.0 12-13 35.960834808734376 39.0 35.0 39.0 30.0 39.0 14-15 36.97573992777303 39.0 36.0 41.0 31.0 41.0 16-17 36.839932645912114 39.0 36.0 41.0 30.5 41.0 18-19 36.76744431007097 39.0 36.0 41.0 30.0 41.0 20-21 36.65632655411781 39.0 36.0 41.0 30.0 41.0 22-23 36.51727732765126 39.0 36.0 40.5 30.0 41.0 24-25 36.642160981389026 39.0 36.0 41.0 30.0 41.0 26-27 36.61530474476177 39.0 36.0 41.0 30.0 41.0 28-29 36.51846404722071 39.0 36.0 41.0 30.0 41.0 30-31 36.329504919890276 39.0 35.5 41.0 30.0 41.0 32-33 36.15319117309026 39.0 35.0 40.0 29.0 41.0 34-35 35.93013736119048 38.0 35.0 40.0 28.5 41.0 36-37 35.71584612711534 38.0 35.0 40.0 27.0 41.0 38-39 35.487867995044425 38.0 34.5 40.0 27.0 41.0 40-41 35.29033382209838 38.0 34.0 40.0 26.0 41.0 42-43 35.03831908137766 38.0 34.0 40.0 25.5 41.0 44-45 34.759326528030655 38.0 33.5 40.0 24.5 41.0 46-47 34.47256147832478 38.0 33.0 40.0 24.0 41.0 48-49 34.352939801176475 38.0 33.0 40.0 23.5 41.0 50-51 33.39950611596184 37.0 32.0 39.5 22.5 40.5 52-53 33.52515614148297 37.5 32.5 39.5 23.0 40.0 54-55 33.91453354931531 38.0 33.0 40.0 23.0 41.0 56-57 33.78786563243392 37.5 33.0 40.0 22.0 41.0 58-59 33.48341324371164 37.0 33.0 40.0 21.5 41.0 60-61 33.1183032912641 36.5 32.0 39.5 20.0 41.0 62-63 32.76052629117895 36.0 32.0 39.0 19.5 41.0 64-65 32.393297126327305 35.5 31.0 39.0 18.0 41.0 66-67 31.969072936247418 35.0 31.0 38.5 17.0 40.0 68-69 31.545715775002698 35.0 31.0 38.0 13.5 40.0 70-71 31.09422976682511 35.0 30.0 37.0 10.0 39.5 72-73 30.698265597782296 35.0 30.0 37.0 8.0 39.0 74-75 30.27994055081311 34.0 29.5 36.0 7.0 39.0 76-77 29.864690342977212 34.0 29.0 36.0 4.5 38.5 78-79 29.429546216353103 34.0 29.0 35.0 2.0 37.0 80-81 28.97643419071484 34.0 29.0 35.0 2.0 37.0 82-83 28.51011271128752 34.0 27.5 35.0 2.0 36.0 84-85 28.204410600048924 34.0 27.0 35.0 2.0 36.0 86-87 27.89228341875647 33.5 27.0 35.0 2.0 35.5 88-89 27.493377799631926 33.0 26.0 35.0 2.0 35.0 90-91 27.076408300441365 33.0 25.5 35.0 2.0 35.0 92-93 26.688563390070442 33.0 25.0 35.0 2.0 35.0 94-95 26.193021094666356 33.0 24.0 35.0 2.0 35.0 96-97 25.57511329455699 32.0 21.5 35.0 2.0 35.0 98-99 24.819677444600476 32.0 10.5 35.0 2.0 35.0 100-101 22.31800860482435 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4999291463315956 1101 2 0.5940031734245501 1101 3 0.5541537123824334 1101 4 0.6193560489175454 1101 5 0.428626390819133 1101 6 0.3874001965910381 1101 7 0.4238851257364544 1101 8 0.5784754698727781 1101 9 0.7018213407700813 1101 10-11 0.6650235749780933 1101 12-13 0.7267175996793895 1101 14-15 0.9396070877433758 1101 16-17 0.8673009491527708 1101 18-19 0.9101785871714085 1101 20-21 0.9817904387309895 1101 22-23 0.9634130485284871 1101 24-25 0.9456380087877534 1101 26-27 0.8805994668641475 1101 28-29 1.0084483652495493 1101 30-31 0.9138519997718575 1101 32-33 0.8764348239787978 1101 34-35 0.956732715436992 1101 36-37 0.7053048760607297 1101 38-39 0.7887526152182076 1101 40-41 0.8127820696505097 1101 42-43 0.905142563181208 1101 44-45 0.9641158090744923 1101 46-47 0.9820884764759441 1101 48-49 0.8166837444538615 1101 50-51 0.8847680726020428 1101 52-53 1.0522888035134343 1101 54-55 1.1273078182517153 1101 56-57 0.7846136318887176 1101 58-59 0.7453783583785807 1101 60-61 0.769391245739051 1101 62-63 0.8518389676098579 1101 64-65 0.842373591086492 1101 66-67 0.8740474861199417 1101 68-69 0.8422294217985424 1101 70-71 0.8790953005606248 1101 72-73 0.7628422933838905 1101 74-75 0.6329090040637482 1101 76-77 0.6803509189026755 1101 78-79 0.7536375274213221 1101 80-81 0.8301927098909658 1101 82-83 0.9784312596046583 1101 84-85 0.8883769269604862 1101 86-87 0.9042315984368408 1101 88-89 0.8693670105624385 1101 90-91 0.680730736430025 1101 92-93 0.9200346065613836 1101 94-95 1.0631011154176981 1101 96-97 0.7624884944922989 1101 98-99 0.71506537043253 1101 100-101 1.0732126698419826 1102 1 0.3625421146782486 1102 2 0.4845960135388623 1102 3 0.27787792894602603 1102 4 0.2306605340879102 1102 5 0.4344691556623417 1102 6 0.3713375907796106 1102 7 0.2870713883906362 1102 8 0.216541915744223 1102 9 0.4279808858858516 1102 10-11 0.3316773674075506 1102 12-13 0.3732262546218621 1102 14-15 0.39147874681712125 1102 16-17 0.33887043795095195 1102 18-19 0.39554169001888084 1102 20-21 0.4345844772599179 1102 22-23 0.3628622121555196 1102 24-25 0.28139980167129863 1102 26-27 0.1258455416614126 1102 28-29 0.2929316600013081 1102 30-31 0.19037346314075165 1102 32-33 0.14972209798040836 1102 34-35 -0.16279100359697907 1102 36-37 0.04067859089925463 1102 38-39 -0.061819691927361475 1102 40-41 0.10073523868719292 1102 42-43 0.18735810012328358 1102 44-45 0.008327584929496368 1102 46-47 0.06064374027030084 1102 48-49 0.21347756580715327 1102 50-51 0.41662995877238984 1102 52-53 0.2994204810471075 1102 54-55 0.34717408248535975 1102 56-57 0.49949991083106937 1102 58-59 0.59875951148247 1102 60-61 0.3853388471822754 1102 62-63 0.4384672419026785 1102 64-65 0.34189592809371305 1102 66-67 0.44809944045383077 1102 68-69 0.41241799441170457 1102 70-71 0.3581377287816139 1102 72-73 0.24177798471808742 1102 74-75 -0.001212249039639346 1102 76-77 0.22872827143301677 1102 78-79 0.21480911591298835 1102 80-81 0.22928476767942385 1102 82-83 0.3571495285174464 1102 84-85 0.3682905256100497 1102 86-87 0.3009452128809933 1102 88-89 0.31966007426893484 1102 90-91 0.24292835638254573 1102 92-93 0.010422980754995592 1102 94-95 0.24244968724113392 1102 96-97 0.12125659040495407 1102 98-99 0.5256440447727364 1102 100-101 0.6843483503173964 1103 1 0.46896087620853777 1103 2 0.5885736144929652 1103 3 0.5946930529607073 1103 4 0.5906767463757063 1103 5 0.6808307083150069 1103 6 0.5448444763432221 1103 7 0.5243028141402206 1103 8 0.46919830841701327 1103 9 0.693751528600572 1103 10-11 0.7804493331940279 1103 12-13 0.7004344800285693 1103 14-15 0.681029740984421 1103 16-17 0.8078602447079319 1103 18-19 0.7484869223018293 1103 20-21 0.8775162924771038 1103 22-23 0.9245802487915569 1103 24-25 0.9773094600951708 1103 26-27 0.7695702681955154 1103 28-29 0.5819869681279428 1103 30-31 0.6902858795170275 1103 32-33 0.8550414055107396 1103 34-35 0.746876737677276 1103 36-37 0.669377135520314 1103 38-39 0.8278836418407849 1103 40-41 0.5210378680593806 1103 42-43 0.5507635191270666 1103 44-45 0.679520026649115 1103 46-47 0.7000181695791383 1103 48-49 0.6207116004306386 1103 50-51 0.6060668147066508 1103 52-53 0.6732884161234196 1103 54-55 0.646432882776061 1103 56-57 0.7664408198372712 1103 58-59 0.635015870612591 1103 60-61 0.590048330438897 1103 62-63 0.5972605445506858 1103 64-65 0.580348400001732 1103 66-67 0.4902374126134177 1103 68-69 0.6608699072753268 1103 70-71 0.6830976639324611 1103 72-73 0.8141965971698717 1103 74-75 0.6363019600056958 1103 76-77 0.6821640651828993 1103 78-79 0.5570169686234223 1103 80-81 0.541821338501034 1103 82-83 0.5077286053487811 1103 84-85 0.5447799130837261 1103 86-87 0.49986765478696427 1103 88-89 0.5175086850435839 1103 90-91 0.47095977467820305 1103 92-93 0.4919078958734211 1103 94-95 0.4187890644656669 1103 96-97 0.8034291301436944 1103 98-99 0.8068149632826973 1103 100-101 1.2251404230677103 1104 1 0.32326575163169835 1104 2 0.470378157802827 1104 3 0.3305727409116379 1104 4 0.3633690170686492 1104 5 0.5430135401190981 1104 6 0.3745514378959527 1104 7 0.655183166317741 1104 8 0.5572491011370886 1104 9 0.6790212733085212 1104 10-11 0.7576007402091847 1104 12-13 0.5359762140722424 1104 14-15 0.7727506146485226 1104 16-17 0.6869634471962698 1104 18-19 0.8828890496592621 1104 20-21 0.9776522298840575 1104 22-23 0.6113549248093193 1104 24-25 0.8390897476823724 1104 26-27 0.45498797510210665 1104 28-29 0.589912451066489 1104 30-31 0.6928915946142027 1104 32-33 0.5266511013865767 1104 34-35 0.40113431558647505 1104 36-37 0.49561637619480337 1104 38-39 0.5489989469956456 1104 40-41 0.6676977244948219 1104 42-43 0.7448817926876359 1104 44-45 0.7543653076627734 1104 46-47 0.6667290798444085 1104 48-49 0.7371579652788469 1104 50-51 0.6159243953643738 1104 52-53 0.6974202261637785 1104 54-55 0.9450348003295161 1104 56-57 0.8402053583830309 1104 58-59 0.798621063490252 1104 60-61 0.846774998907371 1104 62-63 0.9283813925619029 1104 64-65 0.7521231218282125 1104 66-67 0.8415072306153277 1104 68-69 1.0066419905189434 1104 70-71 0.9674670380477828 1104 72-73 0.9087671174196217 1104 74-75 0.7951637733900938 1104 76-77 1.0291082793112274 1104 78-79 0.7398218600796369 1104 80-81 0.7742612488991583 1104 82-83 0.9184211646367295 1104 84-85 1.135974215393862 1104 86-87 1.0784094712863528 1104 88-89 1.0563163525088513 1104 90-91 1.0408522157796298 1104 92-93 1.035045289997992 1104 94-95 1.0200987360628773 1104 96-97 1.1648955134070178 1104 98-99 1.2664775132714752 1104 100-101 1.7715972323064157 1105 1 0.3758569551775537 1105 2 0.46450817257723287 1105 3 0.4301340546972945 1105 4 0.4694492534591532 1105 5 0.5573401681450108 1105 6 0.4889473919816467 1105 7 0.5254763814558245 1105 8 0.4603005842601675 1105 9 0.609779313994963 1105 10-11 0.6136144917718553 1105 12-13 0.4506881131516778 1105 14-15 0.40927578796367214 1105 16-17 0.56019961039663 1105 18-19 0.4212860265884615 1105 20-21 0.49154471766676266 1105 22-23 0.3645450600519595 1105 24-25 0.6464599033818885 1105 26-27 0.5759426290400498 1105 28-29 0.32292472518854964 1105 30-31 0.24395876133769434 1105 32-33 0.3804248256734226 1105 34-35 0.2547864351455189 1105 36-37 0.4776688821613888 1105 38-39 0.3957531236066174 1105 40-41 0.37891831774405205 1105 42-43 0.6731810312374691 1105 44-45 0.3663950837736891 1105 46-47 0.43013287573031533 1105 48-49 0.5721090960891715 1105 50-51 0.7829683776350862 1105 52-53 0.5832623673161734 1105 54-55 0.5184084791566548 1105 56-57 0.8905355095365692 1105 58-59 0.736900981662771 1105 60-61 0.6327313575844968 1105 62-63 0.7549952123141495 1105 64-65 0.8423998578209435 1105 66-67 0.906365737263819 1105 68-69 0.8173971899734269 1105 70-71 0.8651349548565115 1105 72-73 0.8617941659478632 1105 74-75 0.7106763316353479 1105 76-77 0.6343316001431418 1105 78-79 0.9077148024594521 1105 80-81 0.6819246197367548 1105 82-83 0.8049327000384814 1105 84-85 0.9024999569554062 1105 86-87 0.7970201088588027 1105 88-89 0.8961811098676442 1105 90-91 1.1596088926747292 1105 92-93 1.0640059673476934 1105 94-95 0.8614903714759912 1105 96-97 1.1243102196236308 1105 98-99 1.152437849673337 1105 100-101 1.5394361912790266 1106 1 0.0737117406561012 1106 2 0.18169346253480256 1106 3 0.24797469590427568 1106 4 0.25709658962134085 1106 5 0.15925435956415157 1106 6 0.1641807724625508 1106 7 0.26791391092305616 1106 8 0.3741855441847335 1106 9 0.4310617279506417 1106 10-11 0.3767757364677564 1106 12-13 0.3857894804312636 1106 14-15 0.43458696193821567 1106 16-17 0.5346479838053924 1106 18-19 0.4140040841085053 1106 20-21 0.3485649911226787 1106 22-23 0.3689392137530447 1106 24-25 0.23483802690852684 1106 26-27 0.004928956244199867 1106 28-29 0.33201241967228157 1106 30-31 0.16302523941407543 1106 32-33 0.08601417032217995 1106 34-35 0.16350166381123188 1106 36-37 0.11198288499024756 1106 38-39 0.11281582987724903 1106 40-41 0.11599045352906501 1106 42-43 0.03664402039353121 1106 44-45 0.038554442566713476 1106 46-47 0.10122669939838858 1106 48-49 0.16825949684353247 1106 50-51 -0.03239230128995274 1106 52-53 0.2012373791030697 1106 54-55 0.2491557681712706 1106 56-57 -0.08138575401835624 1106 58-59 0.10702120797111547 1106 60-61 0.19639472392112367 1106 62-63 0.3183969096267347 1106 64-65 0.01979023971627214 1106 66-67 0.1529899711157725 1106 68-69 0.26588139553684087 1106 70-71 0.10982142350337298 1106 72-73 0.22142877226563584 1106 74-75 0.12840146129116903 1106 76-77 0.24265786134069245 1106 78-79 0.41152904102616716 1106 80-81 0.37618676023651787 1106 82-83 0.512671973965876 1106 84-85 0.5846593157484286 1106 86-87 0.6575835223430104 1106 88-89 0.8227110485758011 1106 90-91 0.7526970586341832 1106 92-93 0.7905146896484929 1106 94-95 0.6676219132015966 1106 96-97 0.7545270984543713 1106 98-99 1.1808984814508001 1106 100-101 1.512852032863183 1107 1 0.2093914043795806 1107 2 0.06747300973825787 1107 3 0.011992105819651044 1107 4 0.10662325112361515 1107 5 0.21106016295492225 1107 6 0.2510973854940417 1107 7 0.3095778476553832 1107 8 0.30335884298600746 1107 9 0.38595421992463486 1107 10-11 0.27347630570724135 1107 12-13 0.14303922253275658 1107 14-15 0.11868217177060814 1107 16-17 0.239930374636927 1107 18-19 0.37742135810526634 1107 20-21 0.36608474621223763 1107 22-23 0.38581663135077804 1107 24-25 0.31911463284860986 1107 26-27 0.12660566264586492 1107 28-29 0.20688411275828855 1107 30-31 0.20785911166856152 1107 32-33 -0.086204861623834 1107 34-35 0.07249047043810464 1107 36-37 0.08918568506783942 1107 38-39 -0.08213970109819257 1107 40-41 -0.2229228655957698 1107 42-43 -0.04264419258502272 1107 44-45 0.04202113801010654 1107 46-47 -0.012532233910278023 1107 48-49 0.061228727592997245 1107 50-51 -0.046175258020809906 1107 52-53 0.14140107238949184 1107 54-55 0.3474022510523369 1107 56-57 0.24358273932766394 1107 58-59 0.3944428267386755 1107 60-61 0.2771468240435624 1107 62-63 0.19664890376348154 1107 64-65 0.3356092778287305 1107 66-67 0.22049964150671286 1107 68-69 0.22627807747829465 1107 70-71 0.32804659362645694 1107 72-73 0.19066337575712922 1107 74-75 0.24245458517109242 1107 76-77 0.20532809683446018 1107 78-79 0.2285633816731547 1107 80-81 0.36662813602065114 1107 82-83 0.3944558608014255 1107 84-85 0.6189745904055144 1107 86-87 0.4404721654307515 1107 88-89 0.3784881035097847 1107 90-91 0.5501279013681248 1107 92-93 0.45724912292054753 1107 94-95 0.534376708572136 1107 96-97 0.6747487819695479 1107 98-99 0.9418442334802641 1107 100-101 1.2822615448537 1108 1 -0.029662077101715312 1108 2 0.25229223235853127 1108 3 0.17476447566457765 1108 4 0.23717874746568413 1108 5 0.3442746234733889 1108 6 0.1925042477658394 1108 7 -0.07431258705380372 1108 8 -0.09890985555312426 1108 9 0.3072925171593397 1108 10-11 0.0826400872838775 1108 12-13 0.29201018270660484 1108 14-15 0.2797753168134065 1108 16-17 0.25245663930115114 1108 18-19 0.16083887211691916 1108 20-21 0.2308127912100275 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82-83 -1.5033140919639045 2315 84-85 -1.5211104791617451 2315 86-87 -1.5369490687853968 2315 88-89 -1.6649886901920432 2315 90-91 -1.4325073199696838 2315 92-93 -1.3422490951070145 2315 94-95 -1.4868199410106833 2315 96-97 -1.1729153420423941 2315 98-99 -1.172392478160571 2315 100-101 -1.0992450126259925 2316 1 -5.343557612064199 2316 2 -4.593196536390739 2316 3 -5.238010992103636 2316 4 -4.993593649401841 2316 5 -2.540661708704775 2316 6 -4.323261034592619 2316 7 -7.088973196252972 2316 8 -7.420046632949099 2316 9 -7.709423265470377 2316 10-11 -7.262656953643095 2316 12-13 -9.131564828449417 2316 14-15 -10.49930923573757 2316 16-17 -8.48800667464392 2316 18-19 -7.7886120700933645 2316 20-21 -7.207715129779039 2316 22-23 -6.6020980256815776 2316 24-25 -8.355439805465778 2316 26-27 -6.877255956103959 2316 28-29 -6.26221387294904 2316 30-31 -5.466528497739759 2316 32-33 -3.89328616352266 2316 34-35 -4.364419633335444 2316 36-37 -5.520501911168573 2316 38-39 -4.471548543902223 2316 40-41 -4.38205836600676 2316 42-43 -3.7182505479631835 2316 44-45 -3.7466406004343007 2316 46-47 -3.754560933071634 2316 48-49 -3.310603072153569 2316 50-51 -3.2457142181925356 2316 52-53 -3.766919903672054 2316 54-55 -3.9925931089470055 2316 56-57 -3.0987966447501094 2316 58-59 -3.345945694147524 2316 60-61 -2.993393669485453 2316 62-63 -2.927467671778345 2316 64-65 -3.257822597002324 2316 66-67 -3.1043272534241204 2316 68-69 -3.7093222931710166 2316 70-71 -3.8558179073394037 2316 72-73 -3.3952135820730227 2316 74-75 -2.8317547903839504 2316 76-77 -2.978158113276333 2316 78-79 -3.762221673394155 2316 80-81 -3.2703872741971693 2316 82-83 -3.010857261221414 2316 84-85 -2.8361501549337156 2316 86-87 -3.37859419851144 2316 88-89 -3.826913045429645 2316 90-91 -3.829953897575276 2316 92-93 -4.579691547029459 2316 94-95 -4.933666032672324 2316 96-97 -5.81109462649875 2316 98-99 -6.877679934681435 2316 100-101 -6.891623627292162 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5876.0 3 2487.0 4 2687.0 5 2883.0 6 3090.0 7 3723.0 8 4510.0 9 5678.0 10 6916.0 11 8248.0 12 9898.0 13 11344.0 14 12679.0 15 14618.0 16 16967.0 17 19986.0 18 17840.0 19 14890.0 20 15467.0 21 16886.0 22 18965.0 23 21342.0 24 24029.0 25 27445.0 26 32350.0 27 37675.0 28 44871.0 29 53165.0 30 64022.0 31 77268.0 32 94655.0 33 119716.0 34 156419.0 35 208454.0 36 281603.0 37 332448.0 38 214682.0 39 25863.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.470301953608924 17.55820549232166 13.910034329472335 23.061458224597075 2 16.220552686382725 19.644982940866594 38.784493666212086 25.349970706538592 3 20.491949765235333 23.409707544815017 28.62208870397202 27.47625398597763 4 13.501172563299214 15.627249957401625 35.673502433278145 35.19807504602102 5 14.734302371967814 36.93664410041833 32.648170419992255 15.680883107621602 6 33.38402337782829 34.29782305300491 16.863927762746954 15.454225806419842 7 30.18741461790682 31.984242910146765 19.33075213067412 18.49759034127229 8 27.759221040533134 32.73369054847347 19.05255750577754 20.45453090521586 9 27.147614024824424 16.68168580745363 19.495337172324202 36.67536299539774 10-11 26.272388644986027 25.47449017407597 25.850452703898974 22.402668477039025 12-13 27.569736085284756 22.66668997604715 26.392062466513284 23.37151147215481 14-15 24.034494007866407 24.250370853671296 25.13123603528188 26.583899103180414 16-17 23.45341703611868 27.493336320959717 25.580007854830907 23.473238788090697 18-19 23.39985121938787 26.236089369819798 27.61010614839204 22.75395326240029 20-21 24.467222576934038 24.94943100340999 27.398304504861958 23.18504191479401 22-23 23.861412826082944 24.892274934306478 27.556914896890405 23.689397342720174 24-25 24.00352643397978 25.136253060525306 27.531486369279268 23.32873413621565 26-27 23.53381032468279 25.177468792050462 27.858336079778933 23.430384803487815 28-29 23.209181748439644 25.99021859864234 27.567913203415095 23.232686449502918 30-31 23.47544556931368 25.184179151205594 27.425218292782578 23.915156986698143 32-33 23.275852309225037 25.286958011271093 27.525178814994717 23.912010864509156 34-35 24.17774938012776 25.28970443526772 27.177576598586146 23.354969586018377 36-37 24.16053861393157 24.94729714662506 27.451510298254462 23.44065394118891 38-39 23.774860908869837 25.191148773113348 27.469895175844073 23.564095142172743 40-41 23.816172304808653 25.328643454522993 27.39825921227953 23.456925028388827 42-43 23.636455368470532 25.448025762850353 26.831142334333773 24.08437653434534 44-45 23.672736580191522 25.37043372112197 26.929398185178716 24.027431513507793 46-47 24.323785550695227 25.70040671555654 26.49107297992116 23.484734753827077 48-49 24.25227305840057 25.687061188487633 26.259460411965996 23.801205341145803 50-51 23.7962446139625 26.00421235631081 26.58005199734993 23.619491032376764 52-53 24.283092107983375 25.990193800662738 26.05337147785065 23.673342613503237 54-55 23.730985706114403 25.723431674557396 26.301625696899528 24.243956922428673 56-57 24.00237090599033 25.94342711113277 25.959623707287633 24.094578275589267 58-59 24.419583751864778 25.502871529778474 26.008456693963815 24.069088024392926 60-61 23.884957285657883 25.698778479099655 26.73353181694219 23.68273241830027 62-63 23.7296303497636 25.807070093009404 26.67853793843511 23.784761618791887 64-65 23.57027539192282 26.232864912755645 26.271651121010013 23.925208574311522 66-67 23.648100190352615 26.05244024960952 25.99730838353861 24.302151176499255 68-69 23.714386635996313 25.848634664638386 26.37549522406466 24.061483475300644 70-71 24.030882211421908 25.73332207742408 26.028592798859773 24.207202912294235 72-73 23.85856450404177 25.797039029630625 26.311609460043144 24.032787006284465 74-75 23.63434951861087 26.563317948668725 25.90375367603898 23.898578856681425 76-77 23.891524863069634 25.97987602307056 25.911429298545364 24.217169815314442 78-79 23.886995404444338 26.117360922418985 26.069785895148872 23.9258577779878 80-81 24.052606939992902 26.132089357171235 25.80956000242192 24.00574370041394 82-83 23.96501833724132 26.146855940239867 26.01941451271867 23.868711209800143 84-85 23.817707689755313 26.34580933171925 25.816268208283716 24.020214770241726 86-87 23.944996894009428 26.29853540976925 25.71218739509283 24.04428030112849 88-89 23.758327186468676 26.358567843295933 25.36175436808878 24.521350602146615 90-91 23.827664879046452 26.08110003864016 25.674270820771177 24.41696426154221 92-93 24.093606433737737 26.093195857611807 25.819896893208856 23.9933008154416 94-95 23.79461624381525 25.906329947666812 26.062108676400147 24.23694513211779 96-97 23.522527376374665 25.831099502547566 26.235047322098175 24.411325798979597 98-99 23.54558458594747 26.160190686670422 25.615041068381 24.67918365900111 100-101 23.601860263578374 25.904367286344197 25.805002228081708 24.68877022199572 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 207.0 1 164.0 2 179.5 3 232.5 4 218.0 5 225.5 6 346.5 7 624.0 8 1005.5 9 1264.0 10 1206.5 11 1085.5 12 1136.0 13 1255.0 14 1357.0 15 1514.0 16 1666.0 17 1813.5 18 1975.0 19 2176.5 20 2408.0 21 2745.5 22 3278.5 23 3894.0 24 4796.0 25 6065.0 26 7671.5 27 9648.0 28 11615.5 29 13666.5 30 16193.0 31 19616.0 32 23661.5 33 25661.5 34 26655.0 35 28883.0 36 32457.5 37 36320.0 38 39305.5 39 41776.0 40 44842.0 41 48553.5 42 51396.0 43 55025.5 44 58993.0 45 63631.0 46 68161.5 47 75838.5 48 91038.0 49 93049.0 50 82352.5 51 77883.5 52 78163.0 53 80424.5 54 82259.0 55 79925.5 56 74441.5 57 68546.5 58 64416.5 59 59143.0 60 50781.5 61 43718.0 62 37555.5 63 29784.0 64 21813.0 65 16482.0 66 12637.5 67 10196.5 68 8132.5 69 6218.0 70 4967.5 71 3680.5 72 2874.0 73 2448.5 74 1917.0 75 1433.5 76 1050.0 77 669.5 78 434.5 79 294.5 80 201.5 81 132.0 82 78.0 83 57.0 84 36.5 85 22.0 86 17.5 87 18.0 88 15.0 89 8.0 90 5.0 91 5.0 92 4.0 93 4.0 94 3.0 95 2.0 96 2.5 97 1.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03657124181269322 2 0.023675326126386863 3 0.02303545244729533 4 0.052026652215365725 5 0.058326946901805475 6 0.04966404170795082 7 0.0528141890511707 8 0.046366231208017514 9 0.04474193648416977 10-11 0.05559517850260699 12-13 0.048974946976621477 14-15 0.04250237860734939 16-17 0.05185437853253339 18-19 0.05633349428617416 20-21 0.013191241999733223 22-23 0.02158343140627992 24-25 0.006743284156580042 26-27 0.011689999906480002 28-29 0.007013999943888001 30-31 0.01009031570875116 32-33 0.004036126283500463 34-35 0.009056673611757137 36-37 0.010557915705010358 38-39 0.012256042007214822 40-41 0.002067284193988042 42-43 0.002141115772344758 44-45 0.0022641684029392844 46-47 4.922105223781053E-4 48-49 0.0013289684104208842 50-51 4.429894701402948E-4 52-53 0.0038884631267870314 54-55 0.007530820992385011 56-57 0.01771957880561179 58-59 0.011936105167669055 60-61 0.012256042007214822 62-63 0.006694063104342232 64-65 4.9221052237810535E-5 66-67 0.007776926253574063 68-69 0.01838406301082223 70-71 0.006177242055845222 72-73 0.0026333262947228634 74-75 0.004060736809619369 76-77 0.007087831522244716 78-79 0.0060541894252506955 80-81 0.01023797886546459 82-83 0.006374126264796464 84-85 0.017916463014563032 86-87 0.0045775578581163795 88-89 0.006275684160320842 90-91 0.004233010492451706 92-93 0.01767035775337398 94-95 0.01200993674602577 96-97 0.009868820973681011 98-99 0.022912399816700804 100-101 0.03721111549178476 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2031651.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.372558261634765 #Duplication Level Percentage of deduplicated Percentage of total 1 77.5992715169554 42.96870183133028 2 12.533984132170986 13.88077533218087 3 4.134365608606673 6.867912016124163 4 1.8074483509958355 4.0033215648165035 5 1.0295082268361142 2.850325213565752 6 0.6181237062472947 2.0536254562245566 7 0.4099262474204904 1.588906551278509 8 0.29953905411893594 1.32689949806686 9 0.21489605711638143 1.070940999858518 >10 1.2244191764397383 12.260009354271343 >50 0.0818475947757535 3.1218851632198077 >100 0.040849698605734 4.091060747908269 >500 0.003581925975719315 1.3261039463549391 >1k 0.0020596074360386043 1.9997671311881042 >5k 1.790962987859656E-4 0.589765193611488 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6526 0.3212165869039515 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5368 0.2642186084125669 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4300 0.2116505246225853 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4204 0.20692530360775546 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2853 0.14042766203447343 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2727 0.1342258094525093 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2381 0.11719532537822687 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.844210447562106E-5 2 0.0 0.0 0.0 0.0 9.844210447562106E-5 3 0.0 0.0 0.0 0.0 9.844210447562106E-5 4 0.0 0.0 0.0 4.922105223781053E-5 9.844210447562106E-5 5 0.0 9.844210447562106E-5 0.0 4.922105223781053E-5 9.844210447562106E-5 6 0.0 9.844210447562106E-5 0.0 4.922105223781053E-5 9.844210447562106E-5 7 0.0 9.844210447562106E-5 0.0 4.922105223781053E-5 9.844210447562106E-5 8 0.0 9.844210447562106E-5 0.0 4.922105223781053E-5 9.844210447562106E-5 9 0.0 9.844210447562106E-5 0.0 9.844210447562106E-5 9.844210447562106E-5 10-11 4.922105223781053E-5 9.844210447562106E-5 0.0 9.844210447562106E-5 9.844210447562106E-5 12-13 4.922105223781053E-5 9.844210447562106E-5 0.0 9.844210447562106E-5 1.476631567134316E-4 14-15 4.922105223781053E-5 1.7227368283233687E-4 0.0 1.476631567134316E-4 1.968842089512421E-4 16-17 9.844210447562106E-5 1.968842089512421E-4 0.0 1.476631567134316E-4 1.968842089512421E-4 18-19 1.476631567134316E-4 2.2149473507014736E-4 0.0 1.476631567134316E-4 1.968842089512421E-4 20-21 1.968842089512421E-4 2.4610526118905263E-4 0.0 1.968842089512421E-4 2.4610526118905263E-4 22-23 1.968842089512421E-4 2.4610526118905263E-4 0.0 2.707157873079579E-4 2.707157873079579E-4 24-25 2.4610526118905263E-4 2.4610526118905263E-4 0.0 3.4454736566467373E-4 2.953263134268632E-4 26-27 2.953263134268632E-4 2.4610526118905263E-4 0.0 4.183789440213895E-4 2.953263134268632E-4 28-29 2.953263134268632E-4 2.4610526118905263E-4 0.0 8.859789402805894E-4 2.953263134268632E-4 30-31 2.953263134268632E-4 2.4610526118905263E-4 0.0 0.0023133894551770947 2.953263134268632E-4 32-33 2.953263134268632E-4 2.4610526118905263E-4 0.0 0.004479115753640758 3.4454736566467373E-4 34-35 2.953263134268632E-4 2.4610526118905263E-4 0.0 0.007826147305811874 3.937684179024842E-4 36-37 2.953263134268632E-4 2.4610526118905263E-4 0.0 0.011517726223647664 3.937684179024842E-4 38-39 2.953263134268632E-4 2.707157873079579E-4 0.0 0.01565229461162375 3.937684179024842E-4 40-41 3.4454736566467373E-4 2.953263134268632E-4 0.0 0.01838406301082223 3.937684179024842E-4 42-43 3.4454736566467373E-4 2.953263134268632E-4 0.0 0.022001810350301305 4.183789440213895E-4 44-45 3.4454736566467373E-4 2.953263134268632E-4 0.0 0.02552111558530476 4.675999962592E-4 46-47 3.4454736566467373E-4 2.953263134268632E-4 0.0 0.03199368395457684 5.168210484970105E-4 48-49 3.4454736566467373E-4 2.953263134268632E-4 0.0 0.0383185891671355 5.414315746159158E-4 50-51 3.4454736566467373E-4 2.953263134268632E-4 0.0 0.043314525969273265 5.414315746159158E-4 52-53 3.4454736566467373E-4 2.953263134268632E-4 0.0 0.05340484167802442 5.660421007348211E-4 54-55 3.4454736566467373E-4 2.953263134268632E-4 0.0 0.07405307309178594 5.906526268537264E-4 56-57 3.4454736566467373E-4 3.4454736566467373E-4 0.0 0.11067353595671697 5.906526268537264E-4 58-59 3.4454736566467373E-4 3.4454736566467373E-4 0.0 0.14877063038878233 6.152631529726316E-4 60-61 3.4454736566467373E-4 3.4454736566467373E-4 0.0 0.18706460902979893 6.398736790915369E-4 62-63 3.4454736566467373E-4 3.4454736566467373E-4 0.0 0.23197881919680102 6.398736790915369E-4 64-65 3.4454736566467373E-4 3.4454736566467373E-4 0.0 0.2863188608673438 6.398736790915369E-4 66-67 3.4454736566467373E-4 3.4454736566467373E-4 0.0 0.3715943338693506 6.398736790915369E-4 68-69 3.937684179024842E-4 3.4454736566467373E-4 0.0 0.4772965435500487 6.398736790915369E-4 70-71 3.937684179024842E-4 3.4454736566467373E-4 0.0 0.5691676375519221 6.398736790915369E-4 72-73 3.937684179024842E-4 3.4454736566467373E-4 0.0 0.6627860789082377 6.890947313293475E-4 74-75 3.937684179024842E-4 3.4454736566467373E-4 0.0 0.7637138465218682 6.890947313293475E-4 76-77 3.937684179024842E-4 4.429894701402948E-4 0.0 0.8880216139484587 6.890947313293475E-4 78-79 3.937684179024842E-4 4.922105223781053E-4 0.0 0.9895892552411807 6.890947313293475E-4 80-81 3.937684179024842E-4 4.922105223781053E-4 0.0 1.09548834913083 6.890947313293475E-4 82-83 3.937684179024842E-4 4.922105223781053E-4 0.0 1.2121914639866789 7.383157835671579E-4 84-85 3.937684179024842E-4 4.922105223781053E-4 0.0 1.3924881783337788 7.383157835671579E-4 86-87 3.937684179024842E-4 4.922105223781053E-4 0.0 1.6295121553849554 7.629263096860632E-4 88-89 3.937684179024842E-4 4.922105223781053E-4 0.0 1.90052326900634 7.875368358049685E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 10435 0.0 23.77054 2 GTACATG 10675 0.0 23.502047 1 ACATGGG 10735 0.0 22.176687 3 GAGTACT 5955 0.0 21.630482 12-13 GTACTTT 6305 0.0 20.392048 14-15 AGTACTT 6250 0.0 19.811 12-13 GTATCAA 17735 0.0 19.022465 1 TACCTGG 1765 0.0 18.576565 2 CATGGGA 5030 0.0 18.04729 4 GTATATA 900 0.0 17.950478 1 AGAGTAC 13180 0.0 17.921383 10-11 ACTTTTT 7395 0.0 17.642338 16-17 CATGGGG 5685 0.0 17.389194 4 TACTTTT 6910 0.0 17.368488 14-15 GTATAGG 505 0.0 16.936386 1 GTACCGT 170 0.0017812471 16.774473 6 TCAACGC 19915 0.0 16.657955 4 CAACGCA 19930 0.0 16.62198 5 TATAGGG 545 0.0 16.566017 2 ATCAACG 20080 0.0 16.541485 3 >>END_MODULE