FastQCFastQC Report
Thu 26 May 2016
SRR936501_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936501_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2031651
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT76740.377722354872958No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64990.31988761849353065No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57550.2832671556285996No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT36710.18069048276500244No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31920.15711359874309122No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC25950.12772863055711833No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT25530.12566134636313028No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT22180.10917229386346375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGTG8850.024.1493845
TACATGG97350.023.5209452
GTACATG100650.023.0403331
GTAAGGT8250.023.0278554
ACATGGG98750.022.4155673
GAGTACT80750.021.76373512-13
GTACTTT83400.020.95803814-15
CATGGGG51000.019.6498744
AGTACTT84100.019.45662112-13
TATAGGG6350.019.4510632
GTATCAA191300.018.3326021
ACTTTTT96250.018.28292516-17
AGAGTAC148600.018.17151810-11
TACTTTT89600.018.10304314-15
CTATAGG6850.017.3433151
GGTAAGG9400.017.181143
TACCTGG19150.017.1168562
AGGTAAG9500.017.001962
CATGGGA46800.016.8465024
GTACCTG19500.016.5713161
Produced by FastQC (version 0.11.5)