##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936501_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2031651 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.807825261326872 31.0 31.0 34.0 30.0 34.0 2 31.946228953693325 31.0 31.0 34.0 30.0 34.0 3 32.05314052462751 33.0 31.0 34.0 30.0 34.0 4 35.814107344223984 37.0 35.0 37.0 35.0 37.0 5 35.58010504756969 37.0 35.0 37.0 33.0 37.0 6 35.67985446319274 37.0 35.0 37.0 33.0 37.0 7 35.62419775837484 37.0 35.0 37.0 33.0 37.0 8 35.599049246154976 37.0 35.0 37.0 33.0 37.0 9 37.21882941509147 39.0 37.0 39.0 33.0 39.0 10-11 37.08115665535075 39.0 37.0 39.0 33.0 39.0 12-13 36.9828580302424 39.0 37.0 39.0 33.0 39.0 14-15 38.0564100330224 40.0 37.0 41.0 33.0 41.0 16-17 37.976434929030624 40.0 37.0 41.0 33.0 41.0 18-19 38.11712395485248 40.0 37.0 41.0 33.0 41.0 20-21 38.148369478813045 40.0 37.0 41.0 33.0 41.0 22-23 38.0483080017188 40.0 37.0 41.0 33.0 41.0 24-25 37.90872989504595 40.0 37.0 41.0 33.0 41.0 26-27 37.671343897155566 39.5 37.0 41.0 32.0 41.0 28-29 37.591218422849195 39.0 37.0 41.0 32.0 41.0 30-31 37.56802693966631 39.0 37.0 41.0 32.0 41.0 32-33 37.42375659008363 39.0 36.0 41.0 32.0 41.0 34-35 37.24220104732555 39.0 36.0 41.0 31.0 41.0 36-37 36.968649142987644 39.0 35.5 41.0 31.0 41.0 38-39 36.78496085203611 39.0 35.0 41.0 30.5 41.0 40-41 36.56751233356516 39.0 35.0 40.5 30.0 41.0 42-43 36.30269298221003 39.0 35.0 40.0 30.0 41.0 44-45 36.03691775802045 38.5 35.0 40.0 29.5 41.0 46-47 35.67709168553064 38.0 35.0 40.0 28.5 41.0 48-49 35.302361478423215 38.0 34.0 40.0 27.0 41.0 50-51 34.95208330564649 38.0 34.0 40.0 26.0 41.0 52-53 34.64396640958511 38.0 34.0 40.0 26.0 41.0 54-55 34.343436938726185 38.0 33.0 40.0 25.0 41.0 56-57 33.97605445029683 37.0 33.0 40.0 24.0 41.0 58-59 33.60789500755789 36.5 32.5 39.5 23.0 41.0 60-61 33.19710816473892 36.0 32.0 39.0 22.5 40.5 62-63 32.76891306626975 35.5 31.5 39.0 22.0 40.0 64-65 32.32034414375303 35.0 31.0 38.5 20.5 40.0 66-67 31.82885347926391 35.0 31.0 38.0 19.5 40.0 68-69 31.51638962597415 35.0 30.5 37.0 18.5 39.5 70-71 31.066001001156202 34.0 30.0 37.0 18.0 39.0 72-73 30.60894563091791 34.0 30.0 36.0 15.0 39.0 74-75 30.09454921145413 34.0 29.0 36.0 11.0 38.5 76-77 28.702777199430415 32.5 27.0 34.5 9.0 36.0 78-79 29.49728668949539 34.0 29.0 35.0 7.5 37.0 80-81 29.31672836525565 34.0 29.0 35.0 7.0 36.5 82-83 28.98726109947033 34.0 29.0 35.0 3.5 36.0 84-85 28.5894346519161 33.0 28.5 35.0 2.0 36.0 86-87 28.21657902858316 33.0 27.0 35.0 2.0 35.0 88-89 27.80049206285922 33.0 27.0 35.0 2.0 35.0 90-91 27.302125955688254 33.0 26.0 35.0 2.0 35.0 92-93 26.78561253876773 32.0 25.0 34.0 2.0 35.0 94-95 26.156872169481865 32.0 24.5 34.0 2.0 35.0 96-97 25.346221127546023 32.0 21.5 34.0 2.0 35.0 98-99 24.333002813967557 31.0 10.0 34.0 2.0 35.0 100-101 21.07041514512089 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3885564393513512 1101 2 0.4613672273843008 1101 3 0.4133675451912424 1101 4 0.2722366119917865 1101 5 0.2568700808033455 1101 6 0.27474259190881156 1101 7 0.26937524300525695 1101 8 0.29309710068474004 1101 9 0.46924371877262416 1101 10-11 0.4130519018127572 1101 12-13 0.3929489467054239 1101 14-15 0.5345066689563112 1101 16-17 0.5535398835428182 1101 18-19 0.470482468957492 1101 20-21 0.404835860897542 1101 22-23 0.39283014273912187 1101 24-25 0.35258596235416917 1101 26-27 0.2680581017141037 1101 28-29 0.30540023937479077 1101 30-31 0.28104716215440106 1101 32-33 0.5057978831695706 1101 34-35 0.4709748463073353 1101 36-37 0.38587153286673725 1101 38-39 0.4792731087131159 1101 40-41 0.34958991890015767 1101 42-43 0.32125433244729606 1101 44-45 0.48794872475835405 1101 46-47 0.32364882236891646 1101 48-49 0.31284765603487585 1101 50-51 0.3856759467970434 1101 52-53 0.21456119253436157 1101 54-55 0.5314903465570993 1101 56-57 0.4226041574634962 1101 58-59 0.3452162090443309 1101 60-61 0.27165360929020466 1101 62-63 0.34679075967260076 1101 64-65 0.31385170090389636 1101 66-67 0.2315418574659276 1101 68-69 0.1937386429089223 1101 70-71 0.31694969554745 1101 72-73 0.26899033316189147 1101 74-75 0.2422120915948085 1101 76-77 0.06342690409671903 1101 78-79 0.09233957927301972 1101 80-81 0.37643013761157107 1101 82-83 0.409491532269346 1101 84-85 0.3474649375908321 1101 86-87 0.48782303881803557 1101 88-89 0.3535104809673655 1101 90-91 0.2023284990697256 1101 92-93 0.19662706967975652 1101 94-95 0.35113895197696365 1101 96-97 0.6374983634734086 1101 98-99 0.7671289738197729 1101 100-101 1.1015195970268898 1102 1 0.3584102406652683 1102 2 0.36037044059647627 1102 3 0.3090163820676608 1102 4 0.22876199932946406 1102 5 0.11997095394809776 1102 6 0.1840014307101967 1102 7 0.268350322101611 1102 8 0.2704570347423214 1102 9 0.29175249217453114 1102 10-11 0.3683836869470909 1102 12-13 0.30276257052761224 1102 14-15 0.40992164716471535 1102 16-17 0.38643890674605785 1102 18-19 0.3210236805013693 1102 20-21 0.1952775418369228 1102 22-23 0.2169010999407064 1102 24-25 0.3316017372022628 1102 26-27 0.11585290529355063 1102 28-29 -0.0759759631118655 1102 30-31 0.22310729512512495 1102 32-33 0.1792537150756246 1102 34-35 0.05713212095060527 1102 36-37 0.25570657810343533 1102 38-39 0.2544380881554673 1102 40-41 0.3466938325037461 1102 42-43 0.3783868243895867 1102 44-45 0.27208712980077365 1102 46-47 0.11720845435493743 1102 48-49 0.20320271351238262 1102 50-51 0.11246085901367309 1102 52-53 -0.029973508676391702 1102 54-55 0.15446123515094 1102 56-57 0.07276296714361052 1102 58-59 -0.13535780095561734 1102 60-61 0.13369994667255014 1102 62-63 0.08086783727833335 1102 64-65 0.14219423129614484 1102 66-67 0.07309323184215799 1102 68-69 0.17493073969028217 1102 70-71 0.2664124326514141 1102 72-73 0.2518219825614487 1102 74-75 0.11527873489864504 1102 76-77 0.020335186879133715 1102 78-79 -0.0035645623649784852 1102 80-81 0.46899384657648113 1102 82-83 0.15213236326228596 1102 84-85 0.12341619153330541 1102 86-87 0.18337969944656152 1102 88-89 0.04881233041529498 1102 90-91 0.22801186193565215 1102 92-93 0.20693538503851627 1102 94-95 0.44690945656479997 1102 96-97 0.7567201343432473 1102 98-99 0.9733640121463374 1102 100-101 1.404393965860212 1103 1 0.2109986162685651 1103 2 0.25209099293235226 1103 3 0.22931435273646628 1103 4 0.16438337718757623 1103 5 0.12628256530042137 1103 6 0.20126192676893595 1103 7 0.16533614100849547 1103 8 0.28099409006907905 1103 9 0.2848333859001926 1103 10-11 0.2504900730976871 1103 12-13 0.21479320616110442 1103 14-15 0.20094898443620224 1103 16-17 0.3018853037048572 1103 18-19 0.2824752393035297 1103 20-21 0.21605629069554055 1103 22-23 0.1192071581186056 1103 24-25 0.15219786488800935 1103 26-27 0.3342743502369956 1103 28-29 0.36713381457480665 1103 30-31 0.193012537098177 1103 32-33 0.1745558785774861 1103 34-35 0.28794245581820377 1103 36-37 0.37252170211811375 1103 38-39 0.48580141111541764 1103 40-41 0.5322849287898279 1103 42-43 0.4433911103967034 1103 44-45 0.4343325316605373 1103 46-47 0.3454443897294013 1103 48-49 0.22766137217850257 1103 50-51 0.2194540386719055 1103 52-53 0.16288609663825326 1103 54-55 0.09692718002915512 1103 56-57 0.06589650421756943 1103 58-59 0.12614593532450158 1103 60-61 0.08802415515884121 1103 62-63 0.11031550140445745 1103 64-65 0.08575497147093358 1103 66-67 0.3588054544163697 1103 68-69 0.31043378648229947 1103 70-71 0.3429749617683093 1103 72-73 0.23583750536114323 1103 74-75 0.2304740277447408 1103 76-77 0.04806425532479963 1103 78-79 0.03704209399042213 1103 80-81 0.21747045056805803 1103 82-83 0.39486804706676537 1103 84-85 0.36462987694038773 1103 86-87 0.08496132924735633 1103 88-89 0.17923412942019468 1103 90-91 0.17361358006546013 1103 92-93 0.2250335159667145 1103 94-95 0.38172068590345276 1103 96-97 0.19862889829606445 1103 98-99 0.5755452170733797 1103 100-101 0.9138520654819224 1104 1 0.19414604250505718 1104 2 0.29346704137966384 1104 3 0.19719327110072982 1104 4 0.10670995678711392 1104 5 0.10776667654234728 1104 6 0.18463748048196038 1104 7 0.18499999654608246 1104 8 0.1589131368810257 1104 9 0.23346530817193667 1104 10-11 0.28278980878132387 1104 12-13 0.15780462627754588 1104 14-15 0.3286474622120963 1104 16-17 0.3399325462483347 1104 18-19 0.31801605717259207 1104 20-21 0.16177718268313157 1104 22-23 0.1781820045448299 1104 24-25 0.19791600835178969 1104 26-27 0.26051599586982377 1104 28-29 0.23413595384250385 1104 30-31 0.14672281520297048 1104 32-33 0.21239492044207964 1104 34-35 0.30251409838184173 1104 36-37 0.4363250177540081 1104 38-39 0.24669426907628633 1104 40-41 0.2599697784684807 1104 42-43 0.18632064740786802 1104 44-45 0.2753027147580269 1104 46-47 0.2506219214633987 1104 48-49 0.2589010938446563 1104 50-51 0.13574213435718008 1104 52-53 0.28326594262930627 1104 54-55 0.2371077351393538 1104 56-57 0.269096702111284 1104 58-59 0.1444270543401629 1104 60-61 0.34778920218150233 1104 62-63 0.29631956005795246 1104 64-65 0.4166636105393735 1104 66-67 0.17417416888597614 1104 68-69 0.19986296389492963 1104 70-71 0.029836674373498795 1104 72-73 0.12920050748208922 1104 74-75 0.35658431684363734 1104 76-77 0.1750499492583515 1104 78-79 0.32919895224446805 1104 80-81 0.442022555708256 1104 82-83 0.34265823392057726 1104 84-85 0.2870026317399983 1104 86-87 0.4014955323357796 1104 88-89 0.435098448542135 1104 90-91 0.3820430735583322 1104 92-93 0.3167420212736545 1104 94-95 0.4438392709633945 1104 96-97 0.9638765631960418 1104 98-99 1.1032983217600716 1104 100-101 1.461213253577153 1105 1 0.13935799840913887 1105 2 0.1266764969996217 1105 3 0.15131873990399924 1105 4 0.08450402429478743 1105 5 0.019457777803850718 1105 6 0.08207355276290684 1105 7 0.06971416861108537 1105 8 0.12280562466372658 1105 9 -0.033818474076049654 1105 10-11 -0.02525940135620175 1105 12-13 0.0024227647023664645 1105 14-15 0.054604957382004216 1105 16-17 0.11365342362076802 1105 18-19 0.0417102269646179 1105 20-21 0.17051794867927583 1105 22-23 0.1611914374349297 1105 24-25 0.09098453886662128 1105 26-27 0.1161626602634982 1105 28-29 0.06500878030005453 1105 30-31 0.09452199692817231 1105 32-33 0.13846776962949292 1105 34-35 0.1730914367157439 1105 36-37 -0.004180153288153576 1105 38-39 0.24948663184478903 1105 40-41 0.14206831239278728 1105 42-43 0.09090184981722871 1105 44-45 0.2588485722418312 1105 46-47 0.17510027127588046 1105 48-49 0.2523867172109462 1105 50-51 0.4438686260039546 1105 52-53 0.29607364805037406 1105 54-55 0.5171588773145928 1105 56-57 0.2411679602087986 1105 58-59 0.28176425174896025 1105 60-61 0.18561395499432365 1105 62-63 0.3048114341345496 1105 64-65 0.2482899317122289 1105 66-67 0.5177950348934814 1105 68-69 0.4996833980351738 1105 70-71 0.3449491871590382 1105 72-73 0.3156222599539582 1105 74-75 0.15033190179646994 1105 76-77 -0.03150022689802867 1105 78-79 0.2901848597339587 1105 80-81 0.4555218169879467 1105 82-83 0.4019428117135071 1105 84-85 0.3535664458097756 1105 86-87 0.36512264905358904 1105 88-89 0.5253194963884802 1105 90-91 0.4107372433503542 1105 92-93 0.2653611825419837 1105 94-95 0.4559940612804745 1105 96-97 0.5657978042461629 1105 98-99 0.5920510209304304 1105 100-101 0.7156722830123137 1106 1 0.0011778946844813731 1106 2 0.052282343298536205 1106 3 0.020413506523524916 1106 4 0.009072624011906782 1106 5 -0.11190950178124837 1106 6 -0.09296652267320837 1106 7 0.05236386215187139 1106 8 0.04156564352275183 1106 9 -0.04609098704163017 1106 10-11 -0.08915614817373552 1106 12-13 0.06060051104843467 1106 14-15 0.09321881876814331 1106 16-17 -0.011678966761131448 1106 18-19 -0.05216766082416058 1106 20-21 0.006401965652393926 1106 22-23 -0.05762044375318709 1106 24-25 -0.14762460776232444 1106 26-27 -0.03917208749698631 1106 28-29 -0.061794614325116015 1106 30-31 0.07560874846046772 1106 32-33 0.014429580096255279 1106 34-35 -0.03694628134177691 1106 36-37 0.0017580833926587047 1106 38-39 0.25013255357981734 1106 40-41 0.20208009928576587 1106 42-43 0.24272174609257036 1106 44-45 0.026561915004677417 1106 46-47 0.010218140205630277 1106 48-49 -0.08666090183667308 1106 50-51 7.781026659117174E-4 1106 52-53 0.019503633906104767 1106 54-55 0.017851946621526338 1106 56-57 0.08900152927998306 1106 58-59 -0.0062932682887577585 1106 60-61 0.07322357309899274 1106 62-63 0.0922324619516246 1106 64-65 0.22494245410732816 1106 66-67 0.1510906501692908 1106 68-69 0.16353063990221628 1106 70-71 0.24866205844617184 1106 72-73 0.14698718216046913 1106 74-75 0.056454014007691455 1106 76-77 -0.2773913160069412 1106 78-79 0.10292177628275567 1106 80-81 0.23835067130195142 1106 82-83 0.42360476362298627 1106 84-85 0.10764235340053574 1106 86-87 0.053786015390222985 1106 88-89 0.012474140424313163 1106 90-91 0.13387727163889807 1106 92-93 0.05715750503137684 1106 94-95 0.5115103271927808 1106 96-97 0.46099110929095133 1106 98-99 0.7579326805249629 1106 100-101 1.2124120284154252 1107 1 0.029670701146912393 1107 2 0.075498347265615 1107 3 0.052051839683421974 1107 4 0.02581322372387973 1107 5 -0.07016334584996997 1107 6 0.04484505918563286 1107 7 0.0656788760199376 1107 8 -0.10669612699061304 1107 9 0.06128857146696731 1107 10-11 0.04381092432175393 1107 12-13 0.03602403627366613 1107 14-15 0.09292077417193667 1107 16-17 0.030630197981736274 1107 18-19 0.0977690046991384 1107 20-21 -0.014512543081458773 1107 22-23 -0.16457734419216052 1107 24-25 -0.03024032164979218 1107 26-27 0.14160641269636187 1107 28-29 0.03469218499709115 1107 30-31 -0.07194420263066803 1107 32-33 -0.05780769423453336 1107 34-35 -0.17893658975369675 1107 36-37 0.14359005561284732 1107 38-39 0.1883785482843905 1107 40-41 0.17818418108358713 1107 42-43 -0.0868041317579582 1107 44-45 0.20389074169816723 1107 46-47 0.08084764379437104 1107 48-49 0.05233611386334047 1107 50-51 -0.020840729127741042 1107 52-53 -0.002847462628231767 1107 54-55 0.04596256227632267 1107 56-57 -0.22005754906848551 1107 58-59 0.010064498278090639 1107 60-61 0.03786385768019329 1107 62-63 -0.018201411330608153 1107 64-65 -0.021671142635767637 1107 66-67 0.05892517632334915 1107 68-69 0.19633449444103945 1107 70-71 0.026592498434503398 1107 72-73 0.058716200527463513 1107 74-75 -0.030147532484051 1107 76-77 -0.23483420899222907 1107 78-79 -0.13587521552230442 1107 80-81 0.13836987719295735 1107 82-83 0.08214457634306527 1107 84-85 0.20924504967639024 1107 86-87 0.12665567098170527 1107 88-89 0.2581383623544866 1107 90-91 0.2810512436098591 1107 92-93 0.2433746767680134 1107 94-95 0.7212399416489674 1107 96-97 1.0159385853542418 1107 98-99 1.0079969142741483 1107 100-101 1.0688830005418346 1108 1 0.04234837817816839 1108 2 0.046040334573305586 1108 3 0.08405491767009465 1108 4 -0.05678122979764311 1108 5 -0.040783494959278244 1108 6 0.0463980547680336 1108 7 0.0446577401492263 1108 8 0.04999362457606793 1108 9 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-1.1395693257489867 2316 64-65 -1.573146580234642 2316 66-67 -2.4116816127640703 2316 68-69 -1.7648422161518909 2316 70-71 -1.1780368086541415 2316 72-73 -1.1535017565045678 2316 74-75 -1.1896927619346087 2316 76-77 -1.0163474320762127 2316 78-79 -1.2727886237473598 2316 80-81 -1.8832851802930293 2316 82-83 -1.4489762431241928 2316 84-85 -1.4432596327772949 2316 86-87 -1.4381270682158345 2316 88-89 -1.16165215836741 2316 90-91 -1.5094576602806278 2316 92-93 -1.4590162086368217 2316 94-95 -1.5066313628153907 2316 96-97 -1.528292645475922 2316 98-99 -1.613140032752284 2316 100-101 -1.6594759399959393 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 15.0 4 29.0 5 83.0 6 167.0 7 454.0 8 988.0 9 1804.0 10 2850.0 11 4350.0 12 6114.0 13 8416.0 14 10671.0 15 13874.0 16 17121.0 17 21404.0 18 19215.0 19 14490.0 20 13934.0 21 14147.0 22 15366.0 23 17506.0 24 20103.0 25 24486.0 26 29899.0 27 36843.0 28 45410.0 29 55844.0 30 68230.0 31 83630.0 32 103309.0 33 129581.0 34 168744.0 35 225110.0 36 309323.0 37 362172.0 38 178287.0 39 7681.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.91709337360521 18.387010842188765 14.787309631422701 22.908586152783325 2 16.482568785662814 20.326375024119773 37.898025525614216 25.293030664603194 3 20.566316359429994 23.522335162806723 29.0772562821491 26.834092195614183 4 13.946986566491242 16.282887288864735 35.22254970870766 34.547576435936364 5 14.69886539547251 36.93486963442709 32.80666340820414 15.559601561896267 6 33.456632251256124 34.777973349714124 16.342004126699113 15.42339027233064 7 29.994226370572502 32.19061738458032 19.278064982617586 18.537091262229584 8 27.993390597105506 32.852837421387825 18.74313058689706 20.410641394609605 9 26.968442409723476 16.46780227985661 19.39965121413021 37.164104096289705 10-11 26.283438154104378 25.57824518831438 25.705506751310825 22.432809906270418 12-13 27.55545864360403 22.75339487843853 26.460203191876065 23.230943286081374 14-15 23.891955561320007 24.291983421018372 25.272364237499502 26.543696780162122 16-17 23.318078507184616 27.454429334550767 25.844292484116565 23.383199674148052 18-19 23.194633330232406 26.001193118306244 28.181611900862897 22.622561650598456 20-21 24.2122293641969 24.68233963412023 28.0645888491675 23.040842152515367 22-23 23.70940888233144 24.58437673430161 28.264449385772668 23.44176499759428 24-25 23.806278702322768 24.881129930154604 28.29558039603668 23.01701097148595 26-27 23.41088476561058 24.942937798878198 28.46245856006852 23.1837188754427 28-29 23.024859478454513 25.699974255970474 28.308538502091274 22.966627763483743 30-31 23.311795576548043 24.93081895978886 28.133992181631857 23.62339328203124 32-33 23.194582708325896 24.993201313715378 28.216037024230396 23.596178953728327 34-35 24.010116839354072 25.033073430522663 27.806762067464135 23.15004766265913 36-37 23.995372706351453 24.711027972974637 28.129958034384728 23.16364128628918 38-39 23.590826425553374 24.887255038296242 28.16602103259937 23.355897503551006 40-41 23.594134315935168 25.10118807075954 27.96863871219568 23.33603890110961 42-43 23.547060502844992 25.198263471874938 27.453855013683526 23.800821011596543 44-45 23.675094807042534 24.99081401035082 27.54526976292148 23.788821419685164 46-47 24.30106071419782 25.504663696995056 26.927275859523046 23.26699972928408 48-49 24.329579708176073 25.45601180526433 26.6839384614173 23.5304700251423 50-51 23.822276869039275 25.812819447616498 26.858062877528997 23.506840805815227 52-53 24.327179884996934 25.839979169107274 26.413468609439466 23.419372336456327 54-55 23.768630424601035 25.629759358374315 26.603430466637086 23.998179750387568 56-57 24.10417592651385 25.81394364401628 26.24310833565906 23.838772093810803 58-59 24.533016477659928 25.338256803221253 26.27297447689158 23.855752242227236 60-61 23.884430524206717 25.608339738463936 27.007500832482396 23.499728904846958 62-63 23.822395751422373 25.63689654773534 26.907182469744168 23.63352523109812 64-65 23.73073417674163 25.974369911190344 26.585180898945815 23.70971501312221 66-67 23.690852977224736 25.886054764987033 26.3621350764672 24.060957181321037 68-69 23.84065131492322 25.531205353462138 26.70243517061207 23.925708161002568 70-71 24.08278894769173 25.55885665233347 26.342260076548698 24.016094323426103 72-73 23.97952968178913 25.629120932534533 26.52271618106594 23.868633204610397 74-75 23.741763613112063 26.35476442924411 26.03692579105247 23.866546166591355 76-77 23.996633191367877 25.75133607912 26.14095557806111 24.111075151451015 78-79 23.905732275271088 25.844843078684498 26.36712246234511 23.882302183699302 80-81 24.1586803806246 25.920679991267775 26.040489664200656 23.880149963906966 82-83 24.107598393250097 25.876687039407507 26.203579574163527 23.81213499317887 84-85 23.83593221039792 26.171586677269403 26.015646669871117 23.97683444246156 86-87 24.00381377008253 26.076431437416737 25.811982808501043 24.10777198399969 88-89 23.832923409610405 26.10811698196705 25.522852724534456 24.53610688388809 90-91 23.87768418394384 25.86204138153064 25.793739187024205 24.46653524750131 92-93 24.08446530785425 25.93076878777453 25.943369526000588 24.041396378370635 94-95 23.78909311961095 25.658376433440022 26.186283521792475 24.366246925156556 96-97 23.545814154324134 25.59496049101545 26.410095556442094 24.449129798218326 98-99 23.564782774341474 25.90899962295245 25.69684884724849 24.82936875545759 100-101 23.360291204071736 25.722411047640612 25.871950934378173 25.045346813909475 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 301.0 1 222.5 2 217.5 3 296.0 4 263.0 5 255.5 6 377.0 7 694.0 8 1095.5 9 1342.0 10 1326.0 11 1213.0 12 1241.5 13 1395.0 14 1603.0 15 1771.5 16 1918.5 17 2116.0 18 2364.0 19 2613.5 20 2924.5 21 3316.0 22 3848.5 23 4526.5 24 5375.5 25 6532.0 26 8132.0 27 10175.5 28 12206.0 29 14282.0 30 16750.5 31 20062.5 32 24170.5 33 26130.5 34 27007.0 35 29164.5 36 32611.0 37 36420.0 38 39320.0 39 41648.5 40 44309.0 41 48126.0 42 51243.0 43 54421.5 44 57804.0 45 61758.0 46 66046.0 47 75083.5 48 92057.0 49 93497.0 50 81408.5 51 77115.0 52 77715.5 53 80498.5 54 82896.5 55 80212.5 56 74613.0 57 68600.5 58 64256.0 59 58978.0 60 50753.0 61 43659.5 62 37048.5 63 28939.0 64 21019.0 65 16074.5 66 12450.5 67 9944.0 68 7902.0 69 6110.5 70 4794.0 71 3495.5 72 2751.0 73 2393.5 74 1906.5 75 1442.5 76 1084.0 77 698.0 78 461.5 79 328.0 80 224.5 81 160.5 82 106.5 83 65.0 84 44.5 85 33.5 86 24.5 87 10.5 88 2.5 89 2.0 90 2.0 91 1.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0387861891633947 2 0.0060541894252506955 3 0.004971326276018864 4 7.38315783567158E-4 5 8.859789402805895E-4 6 1.476631567134316E-4 7 0.0 8 0.0 9 0.0015750736716099371 10-11 4.183789440213895E-4 12-13 0.001205915779826358 14-15 0.0020918947201069476 16-17 0.0026333262947228634 18-19 0.0 20-21 0.0 22-23 0.0016489052499666528 24-25 0.001033642096994021 26-27 0.004725221014829811 28-29 0.00551275785063478 30-31 0.0012305263059452633 32-33 4.183789440213895E-4 34-35 0.009179726242351664 36-37 0.01112395780574518 38-39 0.009130505190113854 40-41 0.0018703999850368 42-43 5.414315746159158E-4 44-45 0.001205915779826358 46-47 4.9221052237810535E-5 48-49 7.38315783567158E-4 50-51 9.351999925184E-4 52-53 0.002608715768603958 54-55 0.0030270947126253478 56-57 0.001476631567134316 58-59 0.0010582526231129265 60-61 0.0037654104961925053 62-63 0.004946715749899958 64-65 0.008441410458784505 66-67 0.0030024841865064424 68-69 0.003371642078290021 70-71 1.476631567134316E-4 72-73 0.001304357884301979 74-75 0.005758863111823833 76-77 0.0026333262947228634 78-79 0.003716189443954695 80-81 0.004700610488710906 82-83 0.004749831540948716 84-85 0.004946715749899958 86-87 0.0032239789215765895 88-89 0.008638294667735748 90-91 0.011689999906480002 92-93 0.0011813052537074527 94-95 0.002141115772344758 96-97 0.001205915779826358 98-99 0.003790021022311411 100-101 0.004233010492451706 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2031651.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.55950634246502 #Duplication Level Percentage of deduplicated Percentage of total 1 75.6024161596087 39.736256716466336 2 13.580421546671909 14.275605048313016 3 4.459297204884677 7.031353791692181 4 2.001368187309943 4.207636957380988 5 1.104277636990505 2.9020143732622365 6 0.6863554927334864 2.1644703524106834 7 0.4674921373718115 1.7199809171472378 8 0.34434683877427785 1.447895988525155 9 0.23913684905179491 1.1312023261009425 >10 1.3676985679719322 13.096475010062194 >50 0.09434681502011676 3.4140268894343655 >100 0.04616846145842689 4.44130134140863 >500 0.004512069060897246 1.6406480269436283 >1k 0.0018800287753738508 1.8065556992559924 >5k 2.8200431630607766E-4 0.9845765615964286 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7674 0.377722354872958 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6499 0.31988761849353065 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5755 0.2832671556285996 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3671 0.18069048276500244 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3192 0.15711359874309122 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2595 0.12772863055711833 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2553 0.12566134636313028 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2218 0.10917229386346375 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 4.922105223781053E-5 0.0 0.0 0.0 4 0.0 4.922105223781053E-5 0.0 0.0 0.0 5 0.0 9.844210447562106E-5 0.0 0.0 0.0 6 0.0 9.844210447562106E-5 0.0 0.0 0.0 7 0.0 9.844210447562106E-5 0.0 0.0 0.0 8 0.0 9.844210447562106E-5 0.0 0.0 0.0 9 0.0 9.844210447562106E-5 0.0 4.922105223781053E-5 0.0 10-11 0.0 9.844210447562106E-5 0.0 4.922105223781053E-5 0.0 12-13 0.0 9.844210447562106E-5 0.0 4.922105223781053E-5 7.38315783567158E-5 14-15 0.0 2.2149473507014736E-4 0.0 4.922105223781053E-5 1.2305263059452632E-4 16-17 0.0 2.4610526118905263E-4 0.0 4.922105223781053E-5 2.4610526118905263E-4 18-19 0.0 2.953263134268632E-4 0.0 4.922105223781053E-5 2.4610526118905263E-4 20-21 0.0 3.4454736566467373E-4 0.0 9.844210447562106E-5 2.4610526118905263E-4 22-23 0.0 3.4454736566467373E-4 0.0 1.7227368283233687E-4 2.4610526118905263E-4 24-25 0.0 3.4454736566467373E-4 0.0 2.4610526118905263E-4 2.4610526118905263E-4 26-27 0.0 3.4454736566467373E-4 0.0 3.4454736566467373E-4 3.937684179024842E-4 28-29 0.0 3.4454736566467373E-4 0.0 8.36757888042779E-4 3.937684179024842E-4 30-31 0.0 3.4454736566467373E-4 0.0 0.0023133894551770947 4.429894701402948E-4 32-33 0.0 3.4454736566467373E-4 0.0 0.0046021683842352844 4.429894701402948E-4 34-35 4.922105223781053E-5 3.4454736566467373E-4 0.0 0.0078015367796929695 4.429894701402948E-4 36-37 9.844210447562106E-5 3.4454736566467373E-4 0.0 0.011566947275885475 4.429894701402948E-4 38-39 9.844210447562106E-5 3.4454736566467373E-4 0.0 0.015775347242218275 4.429894701402948E-4 40-41 9.844210447562106E-5 3.937684179024842E-4 0.0 0.018482505115297854 4.429894701402948E-4 42-43 9.844210447562106E-5 3.937684179024842E-4 0.0 0.02222330508537145 4.675999962592E-4 44-45 9.844210447562106E-5 3.937684179024842E-4 0.0 0.025841052424850526 4.922105223781053E-4 46-47 9.844210447562106E-5 3.937684179024842E-4 0.0 0.03236284184636042 4.922105223781053E-4 48-49 9.844210447562106E-5 3.937684179024842E-4 0.0 0.03856469442832455 4.922105223781053E-4 50-51 9.844210447562106E-5 3.937684179024842E-4 0.0 0.043585241756581225 5.414315746159158E-4 52-53 9.844210447562106E-5 3.937684179024842E-4 0.0 0.0533310100996677 6.152631529726316E-4 54-55 9.844210447562106E-5 3.937684179024842E-4 0.0 0.07375774677835908 6.398736790915369E-4 56-57 9.844210447562106E-5 5.414315746159158E-4 0.0 0.11020593596045777 6.890947313293475E-4 58-59 9.844210447562106E-5 5.414315746159158E-4 0.0 0.14832764091864203 7.383157835671579E-4 60-61 9.844210447562106E-5 5.660421007348211E-4 0.0 0.18610479851116163 7.383157835671579E-4 62-63 9.844210447562106E-5 5.906526268537264E-4 0.0 0.2309943981520448 7.383157835671579E-4 64-65 9.844210447562106E-5 5.906526268537264E-4 0.0 0.28491606087856625 7.383157835671579E-4 66-67 9.844210447562106E-5 5.906526268537264E-4 0.0 0.3701177023022163 7.383157835671579E-4 68-69 9.844210447562106E-5 5.906526268537264E-4 0.0 0.4754261435650119 7.383157835671579E-4 70-71 9.844210447562106E-5 5.906526268537264E-4 0.0 0.5672234059885286 7.383157835671579E-4 72-73 9.844210447562106E-5 5.906526268537264E-4 0.0 0.6614324999716978 8.36757888042779E-4 74-75 9.844210447562106E-5 5.906526268537264E-4 0.0 0.7623602675853284 8.613684141616843E-4 76-77 9.844210447562106E-5 5.906526268537264E-4 0.0 0.8869633613253458 9.105894663994948E-4 78-79 1.476631567134316E-4 5.906526268537264E-4 0.0 0.9887032763009002 9.351999925184001E-4 80-81 1.476631567134316E-4 6.152631529726316E-4 0.0 1.0955129596569488 9.351999925184001E-4 82-83 1.968842089512421E-4 6.398736790915369E-4 0.0 1.2121668534605599 9.351999925184001E-4 84-85 2.4610526118905263E-4 6.398736790915369E-4 0.0 1.3918483046546872 9.351999925184001E-4 86-87 2.4610526118905263E-4 6.398736790915369E-4 0.0 1.6280109132917022 0.001009031570875116 88-89 2.4610526118905263E-4 6.398736790915369E-4 0.0 1.8967824690362665 0.0010828631492318316 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGGTG 885 0.0 24.149384 5 TACATGG 9735 0.0 23.520945 2 GTACATG 10065 0.0 23.040333 1 GTAAGGT 825 0.0 23.027855 4 ACATGGG 9875 0.0 22.415567 3 GAGTACT 8075 0.0 21.763735 12-13 GTACTTT 8340 0.0 20.958038 14-15 CATGGGG 5100 0.0 19.649874 4 AGTACTT 8410 0.0 19.456621 12-13 TATAGGG 635 0.0 19.451063 2 GTATCAA 19130 0.0 18.332602 1 ACTTTTT 9625 0.0 18.282925 16-17 AGAGTAC 14860 0.0 18.171518 10-11 TACTTTT 8960 0.0 18.103043 14-15 CTATAGG 685 0.0 17.343315 1 GGTAAGG 940 0.0 17.18114 3 TACCTGG 1915 0.0 17.116856 2 AGGTAAG 950 0.0 17.00196 2 CATGGGA 4680 0.0 16.846502 4 GTACCTG 1950 0.0 16.571316 1 >>END_MODULE