FastQCFastQC Report
Thu 26 May 2016
SRR936500_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936500_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2081399
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT65470.31454805157492627No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53490.25699061064216905No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT43410.20856164531644342No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43060.20688008402041128No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT29210.1403383013059966No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC27610.13265116395270682No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22800.1095417072843794No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG1201.7035443E-423.7662565
TACATGG102200.023.7622572
GTACATG104000.023.7177011
ACATGGG106650.022.2832743
GAGTACT61500.021.21420112-13
TAAGGTG9850.020.7502335
GTACTTT64900.019.91758314-15
GTATCAA175900.019.3187371
AGTACTT65300.019.28821812-13
CATGGGG55850.019.2333584
ATAGGAC6100.018.7004173
GGTAAGG9250.018.4982513
GTAAGGT9250.018.4982514
AGAGTAC129850.018.39613710-11
GTATAGG5750.018.1841661
GTACACG4500.017.9545671
AGGTAAG9100.017.7564132
CATGGGA48300.017.5163154
ATTCGCG3150.017.33812980-81
TACTTTT71300.017.29658114-15
Produced by FastQC (version 0.11.5)