##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936500_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2081399 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.125052909125063 31.0 31.0 34.0 28.0 34.0 2 31.157938963168522 31.0 31.0 34.0 27.0 34.0 3 31.09702993034973 31.0 31.0 34.0 27.0 34.0 4 34.763252985131636 37.0 35.0 37.0 32.0 37.0 5 34.80158297375947 37.0 35.0 37.0 32.0 37.0 6 34.860021552811354 37.0 35.0 37.0 32.0 37.0 7 34.713098257470094 37.0 35.0 37.0 32.0 37.0 8 34.691799602094555 37.0 35.0 37.0 31.0 37.0 9 36.14252625277518 39.0 35.0 39.0 31.0 39.0 10-11 36.00405808785341 39.0 35.0 39.0 30.0 39.0 12-13 35.887162432575394 38.5 35.0 39.0 30.0 39.0 14-15 36.83373178328614 39.0 36.0 41.0 30.0 41.0 16-17 36.73420401374268 39.0 36.0 41.0 30.0 41.0 18-19 36.58463898560535 39.0 36.0 41.0 30.0 41.0 20-21 36.55003341502518 39.0 36.0 41.0 30.0 41.0 22-23 36.545444674471355 39.0 36.0 40.5 30.0 41.0 24-25 36.64712316091244 39.0 36.0 41.0 30.0 41.0 26-27 36.56556527604751 39.0 36.0 41.0 30.0 41.0 28-29 36.45062551677982 39.0 36.0 41.0 30.0 41.0 30-31 36.24952279692649 39.0 35.0 40.5 29.5 41.0 32-33 36.084603432595095 39.0 35.0 40.0 29.0 41.0 34-35 35.873240546382505 38.0 35.0 40.0 28.5 41.0 36-37 35.65513147647327 38.0 35.0 40.0 27.0 41.0 38-39 35.43547681150995 38.0 34.5 40.0 26.5 41.0 40-41 35.187019884222096 38.0 34.0 40.0 25.5 41.0 42-43 34.88699691889926 38.0 34.0 40.0 25.0 41.0 44-45 34.728375722290636 38.0 33.0 40.0 24.0 41.0 46-47 34.581652052297514 38.0 33.0 40.0 24.0 41.0 48-49 34.32154310634338 38.0 33.0 40.0 23.0 41.0 50-51 33.3103114780011 37.0 32.0 39.5 22.0 40.5 52-53 33.39672114765117 37.0 32.5 39.5 22.0 40.0 54-55 33.80998813778617 38.0 33.0 40.0 22.0 41.0 56-57 33.621712127275934 37.0 33.0 40.0 21.5 41.0 58-59 33.35016544160922 37.0 32.5 40.0 20.5 41.0 60-61 33.00867805740273 36.5 32.0 39.5 20.0 41.0 62-63 32.64731365778498 36.0 31.5 39.0 18.5 41.0 64-65 32.26310548818367 35.0 31.0 39.0 17.5 40.5 66-67 31.86930977674151 35.0 31.0 38.5 15.0 40.0 68-69 31.41748242408111 35.0 30.5 38.0 12.5 40.0 70-71 30.99680815643709 35.0 30.0 37.0 9.0 39.5 72-73 30.575009404732107 35.0 30.0 36.5 7.5 39.0 74-75 30.17343070694278 34.0 29.5 36.0 6.5 39.0 76-77 29.72588124621949 34.0 29.0 35.5 2.0 38.0 78-79 29.277917400748247 34.0 29.0 35.0 2.0 37.0 80-81 28.84332004579612 34.0 28.5 35.0 2.0 37.0 82-83 28.46436795636012 34.0 27.5 35.0 2.0 36.0 84-85 28.101807486214803 33.5 27.0 35.0 2.0 36.0 86-87 27.76700983329001 33.0 26.5 35.0 2.0 35.5 88-89 27.407200397425 33.0 26.0 35.0 2.0 35.0 90-91 27.034979597857017 33.0 25.0 35.0 2.0 35.0 92-93 26.598560391352166 33.0 25.0 35.0 2.0 35.0 94-95 26.079409329974695 33.0 24.0 35.0 2.0 35.0 96-97 25.44342194841066 32.0 21.5 35.0 2.0 35.0 98-99 24.66117668933251 32.0 10.0 34.5 2.0 35.0 100-101 22.061585981351964 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6011778833169217 1101 2 0.5257213961169249 1101 3 0.6673586328614611 1101 4 0.7131168483048782 1101 5 0.6545723160857548 1101 6 0.583717323353774 1101 7 0.7533210732011426 1101 8 0.6828614223575187 1101 9 0.9299612979595793 1101 10-11 0.8991363190124062 1101 12-13 1.0150937471651176 1101 14-15 1.1494808769920368 1101 16-17 1.2711105481947556 1101 18-19 1.3110612917101676 1101 20-21 1.233562992175905 1101 22-23 1.1475550136342534 1101 24-25 1.1452937539095913 1101 26-27 1.0432860741450298 1101 28-29 1.0450493171981563 1101 30-31 0.9865152221774238 1101 32-33 0.9386094395652336 1101 34-35 0.8433136087458308 1101 36-37 0.7982440357186107 1101 38-39 0.8092797094236559 1101 40-41 0.8143743982696847 1101 42-43 0.9102762790271655 1101 44-45 0.8167066282360835 1101 46-47 0.7815265088817256 1101 48-49 0.9859470390243317 1101 50-51 0.8288708253025732 1101 52-53 0.8168188080019974 1101 54-55 0.7792997150900902 1101 56-57 0.9599244760106771 1101 58-59 0.860191716897539 1101 60-61 0.7560524382835183 1101 62-63 0.8756943431817064 1101 64-65 0.7501136155965824 1101 66-67 0.9164043007769003 1101 68-69 1.027405016471608 1101 70-71 0.7405351298503327 1101 72-73 0.7625749058455078 1101 74-75 0.7884990881500791 1101 76-77 0.830402359754423 1101 78-79 0.8267136807115527 1101 80-81 0.942240279317307 1101 82-83 1.0293858855027374 1101 84-85 1.2236607794230494 1101 86-87 1.1476091561450872 1101 88-89 1.1504033872955297 1101 90-91 1.0798722214073244 1101 92-93 1.094709822279551 1101 94-95 1.3758467394919514 1101 96-97 1.315649009182021 1101 98-99 1.303245274901034 1101 100-101 1.7603963936588372 1102 1 0.3327770736003224 1102 2 0.43622746899141873 1102 3 0.570334341363484 1102 4 0.48046502644253053 1102 5 0.38133344968898797 1102 6 0.44385902375863395 1102 7 0.5868838262375817 1102 8 0.5036306531267485 1102 9 0.5882406501863002 1102 10-11 0.5989541327775854 1102 12-13 0.4557010346145063 1102 14-15 0.6647845207167364 1102 16-17 0.6155639894903118 1102 18-19 0.6271645305765645 1102 20-21 0.8445447735524212 1102 22-23 0.5806015723387006 1102 24-25 0.8336338348812475 1102 26-27 0.5826383008656819 1102 28-29 0.5192092362264944 1102 30-31 0.4743289873596055 1102 32-33 0.6282551885530907 1102 34-35 0.4275646208915731 1102 36-37 0.5998837118319784 1102 38-39 0.5326299118528013 1102 40-41 0.7041719691198907 1102 42-43 0.5019159551405252 1102 44-45 0.6943584500984272 1102 46-47 0.8303119339829337 1102 48-49 1.0595603993482214 1102 50-51 1.0947007848167445 1102 52-53 1.0250677958562449 1102 54-55 0.9747146948471723 1102 56-57 0.9928293343102723 1102 58-59 0.8098880731728499 1102 60-61 0.8649491994171186 1102 62-63 0.9975870557323105 1102 64-65 1.2394759637747228 1102 66-67 1.215220090250579 1102 68-69 1.1014535994675576 1102 70-71 0.763784117704585 1102 72-73 1.117524298558056 1102 74-75 0.9847035415913723 1102 76-77 1.0090663273657583 1102 78-79 1.0435760288896923 1102 80-81 1.22976052223229 1102 82-83 1.0976449948144804 1102 84-85 1.0862113867104952 1102 86-87 0.8260403302341572 1102 88-89 1.0221341565263025 1102 90-91 1.294477484565217 1102 92-93 1.2526450449516169 1102 94-95 1.3704418811923595 1102 96-97 0.9651429363075259 1102 98-99 1.1066562060751224 1102 100-101 1.6573801993268518 1103 1 0.4844218356703891 1103 2 0.5494984859002088 1103 3 0.6738895663767046 1103 4 0.6362401100984485 1103 5 0.561499429482403 1103 6 0.5410810217323174 1103 7 0.6418313318987359 1103 8 0.5740499620017445 1103 9 0.5863379574547807 1103 10-11 0.574826616540868 1103 12-13 0.5956798834929771 1103 14-15 0.8875786888197155 1103 16-17 1.0323258496587897 1103 18-19 1.0314115567046613 1103 20-21 0.9453473758669304 1103 22-23 0.8536682528639332 1103 24-25 0.7302097952592206 1103 26-27 0.6411474898977758 1103 28-29 0.6006433246494822 1103 30-31 0.7875038352854631 1103 32-33 0.9009489998318045 1103 34-35 0.6388603894563261 1103 36-37 0.7985145399952529 1103 38-39 0.587635694101202 1103 40-41 0.8060732490856495 1103 42-43 0.7990801306302515 1103 44-45 0.7616510056302133 1103 46-47 1.0098966615815144 1103 48-49 1.1127033490416522 1103 50-51 1.0372239239015144 1103 52-53 1.1334150718420517 1103 54-55 1.0682541675222623 1103 56-57 1.1446539717340372 1103 58-59 1.0050243243383719 1103 60-61 0.945668327458101 1103 62-63 0.9864224221028337 1103 64-65 0.9738363946279094 1103 66-67 0.8840852054313579 1103 68-69 0.8847010231883026 1103 70-71 0.9405970493549773 1103 72-73 0.9308088881092438 1103 74-75 0.9269206655232232 1103 76-77 0.972969657860105 1103 78-79 0.818725277500139 1103 80-81 0.9610905705488797 1103 82-83 0.9971029973251682 1103 84-85 0.917778426481874 1103 86-87 0.9551830135203332 1103 88-89 0.8631614289600122 1103 90-91 0.8191698548228707 1103 92-93 1.0931584238432812 1103 94-95 1.3244674562889323 1103 96-97 1.4104921114541504 1103 98-99 1.5207587869963248 1103 100-101 1.7294539578084915 1104 1 0.46911349415610104 1104 2 0.5463965739945067 1104 3 0.6003838132621269 1104 4 0.6451075633475156 1104 5 0.6356547200823215 1104 6 0.42952546787615375 1104 7 0.5489278754416489 1104 8 0.6637253270857784 1104 9 0.7325383782441861 1104 10-11 0.72041501911837 1104 12-13 0.6884867935394396 1104 14-15 0.8258864883358328 1104 16-17 0.7699691039802019 1104 18-19 0.8120662369591969 1104 20-21 0.8890973818413386 1104 22-23 0.6165538530145795 1104 24-25 0.8635707383169162 1104 26-27 0.2885449427931661 1104 28-29 0.35229082701295766 1104 30-31 0.47513055244940716 1104 32-33 0.658178748744227 1104 34-35 0.6004557089628122 1104 36-37 0.6619623079091568 1104 38-39 0.35154291778011526 1104 40-41 0.7117708255795705 1104 42-43 0.9072730494767782 1104 44-45 0.8148246582103482 1104 46-47 0.918633538199991 1104 48-49 1.172400336698054 1104 50-51 0.9257515337851885 1104 52-53 0.8348777977582671 1104 54-55 0.8551944014704702 1104 56-57 0.8861918222285681 1104 58-59 0.8626807414724027 1104 60-61 0.9477469430016896 1104 62-63 0.8135252459419604 1104 64-65 0.7523230822161509 1104 66-67 0.783081648508638 1104 68-69 0.7079792708996244 1104 70-71 0.8784460144443678 1104 72-73 0.8965172280940799 1104 74-75 0.7685742761200025 1104 76-77 0.9059967988723514 1104 78-79 0.7799843574032828 1104 80-81 1.0169175765873284 1104 82-83 0.8980509205736347 1104 84-85 0.9170452473041593 1104 86-87 0.8608218018533194 1104 88-89 0.8042702692655581 1104 90-91 1.2477152349815306 1104 92-93 1.0569384643545412 1104 94-95 1.1727794235510949 1104 96-97 1.0285937031316585 1104 98-99 0.8404509568912459 1104 100-101 1.5479010613683784 1105 1 0.5147089658331616 1105 2 0.5080929044278264 1105 3 0.49444549596007903 1105 4 0.4889922712592636 1105 5 0.6066425079897257 1105 6 0.49812518721802945 1105 7 0.554814812241375 1105 8 0.5493130511949786 1105 9 0.5466075475582386 1105 10-11 0.7214401546220799 1105 12-13 0.7045454500967736 1105 14-15 1.0968334912823394 1105 16-17 0.9432859538604959 1105 18-19 0.7384194630653482 1105 20-21 0.880678875502241 1105 22-23 0.8049817221229816 1105 24-25 0.6375969720676053 1105 26-27 0.6232649572984883 1105 28-29 0.6294126010530263 1105 30-31 0.578512554786279 1105 32-33 0.6303463310666331 1105 34-35 0.5961191855691581 1105 36-37 0.6599147364027544 1105 38-39 0.4792582221061181 1105 40-41 0.4868900197965118 1105 42-43 0.3665297058838419 1105 44-45 0.3514564368260622 1105 46-47 0.7733477442789933 1105 48-49 1.0171114293525747 1105 50-51 1.1612770343963987 1105 52-53 1.0311825858181933 1105 54-55 0.9696189375949658 1105 56-57 0.8704194252561521 1105 58-59 0.9674622101179224 1105 60-61 0.8567839950026226 1105 62-63 0.9380857334358481 1105 64-65 0.888227547775692 1105 66-67 0.9729274305158349 1105 68-69 0.9617323646402447 1105 70-71 1.163579907444248 1105 72-73 1.031486091599664 1105 74-75 1.0091219733781642 1105 76-77 1.0023239927905543 1105 78-79 1.166322092872882 1105 80-81 1.3162606305238107 1105 82-83 1.058281264494564 1105 84-85 1.1615537874005213 1105 86-87 1.158581148538083 1105 88-89 1.093784552550293 1105 90-91 1.155107840958312 1105 92-93 1.1562634541301797 1105 94-95 1.1323325751510218 1105 96-97 1.1652433434456455 1105 98-99 1.5185428865980377 1105 100-101 1.7824371948641016 1106 1 0.24383778529460898 1106 2 0.1847485615973099 1106 3 0.1549048732870446 1106 4 0.3506889488885818 1106 5 0.47118660567026893 1106 6 0.28047421323875454 1106 7 0.4698533491413457 1106 8 0.5551989343640216 1106 9 0.5718234756311844 1106 10-11 0.5993929502958011 1106 12-13 0.7000842651071366 1106 14-15 0.394677537337337 1106 16-17 0.2656717875120478 1106 18-19 0.5452123396622923 1106 20-21 0.2025437325152879 1106 22-23 0.24663266799476702 1106 24-25 -0.05178885082406026 1106 26-27 -0.10119450610766023 1106 28-29 0.14668454119637886 1106 30-31 0.21352455646306367 1106 32-33 0.17133473079987738 1106 34-35 0.0638592580820827 1106 36-37 0.037093258682432406 1106 38-39 0.15772736107501117 1106 40-41 0.17724401381533283 1106 42-43 0.11399558548234978 1106 44-45 0.06645652356400689 1106 46-47 0.21990421496255408 1106 48-49 0.1483120120558894 1106 50-51 -0.023074474305836645 1106 52-53 0.1738275265695819 1106 54-55 0.3403061678152568 1106 56-57 0.5011852202906582 1106 58-59 0.36963498463638444 1106 60-61 0.5695277972471402 1106 62-63 0.4490252546907101 1106 64-65 0.31894018013615266 1106 66-67 0.20002262947182103 1106 68-69 0.5317263720364664 1106 70-71 0.3874163947814928 1106 72-73 0.4784706524652158 1106 74-75 0.5010597363780427 1106 76-77 0.32050764754103866 1106 78-79 0.46311825672810514 1106 80-81 0.6077864212280062 1106 82-83 0.6898670075873135 1106 84-85 0.8526032220996953 1106 86-87 0.976417741717583 1106 88-89 0.8882582256079736 1106 90-91 0.8621002587444657 1106 92-93 0.959254683496706 1106 94-95 1.1010661814051126 1106 96-97 1.188160276822341 1106 98-99 1.3253124866834156 1106 100-101 1.7525661840684137 1107 1 0.32189397873257164 1107 2 0.4058323000798296 1107 3 0.41289235818294046 1107 4 0.49608066110663884 1107 5 0.4839269697498878 1107 6 0.48037395129966143 1107 7 0.421076702055295 1107 8 0.47264391674061557 1107 9 0.7088795062869409 1107 10-11 0.7152824063014691 1107 12-13 0.5815601174505076 1107 14-15 0.47550315339147886 1107 16-17 0.5418051320658819 1107 18-19 0.8652180391646382 1107 20-21 0.593598127698634 1107 22-23 0.6440199498166734 1107 24-25 0.5504835335357612 1107 26-27 0.45417163272814065 1107 28-29 0.4566242012748276 1107 30-31 0.35897470876856374 1107 32-33 0.7086120687540145 1107 34-35 0.44885139735905 1107 36-37 0.5535088667334804 1107 38-39 0.6627722281865047 1107 40-41 0.6604322882261826 1107 42-43 0.6523119881910802 1107 44-45 0.7116076751312477 1107 46-47 0.7907693025143061 1107 48-49 0.8294919025455343 1107 50-51 0.8020504706011096 1107 52-53 0.8186174121417693 1107 54-55 0.924074082889689 1107 56-57 1.1060538320984463 1107 58-59 1.1578170813987114 1107 60-61 1.0202701351141528 1107 62-63 1.1420533947616036 1107 64-65 1.114554793473154 1107 66-67 1.051490346955017 1107 68-69 0.9210103513346475 1107 70-71 1.0985283417629468 1107 72-73 1.050834143858797 1107 74-75 0.9623267089732543 1107 76-77 1.0644305159760385 1107 78-79 1.107057148372533 1107 80-81 1.2122895168525645 1107 82-83 1.0875433022052583 1107 84-85 1.0616716786056095 1107 86-87 1.2498915310196033 1107 88-89 1.2961087269389182 1107 90-91 1.33803537452172 1107 92-93 1.3491146695475855 1107 94-95 1.240608678399166 1107 96-97 1.5000615049992199 1107 98-99 1.7290129980235562 1107 100-101 2.11317903145223 1108 1 0.09580862723224115 1108 2 0.08066699623481455 1108 3 0.2568362466345775 1108 4 0.10070090410467003 1108 5 0.23476265427373022 1108 6 0.2105655685584793 1108 7 0.3606359539634809 1108 8 0.3930630580379457 1108 9 0.038802076995366974 1108 10-11 0.2591770882242841 1108 12-13 0.08388884503578709 1108 14-15 0.6259914254116197 1108 16-17 0.7243765059028391 1108 18-19 0.6372681586207918 1108 20-21 0.6082338913089416 1108 22-23 0.6081157130963888 1108 24-25 0.5443001885683287 1108 26-27 0.5405119257549273 1108 28-29 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0.5737946361815922 2311 84-85 0.5075773339317955 2311 86-87 0.5464416512455585 2311 88-89 0.6303811918337949 2311 90-91 0.5777632983594607 2311 92-93 0.6034924956520804 2311 94-95 0.6305383642469771 2311 96-97 0.6721523975317645 2311 98-99 0.5318919420399446 2311 100-101 0.33718773270631885 2312 1 0.19463183068534207 2312 2 0.14227684724474443 2312 3 0.36392348248552153 2312 4 0.3143339535680383 2312 5 0.300009346160941 2312 6 0.30459138350414605 2312 7 0.30196299049438124 2312 8 0.2609958208537577 2312 9 0.18199607239566973 2312 10-11 0.20140135660638947 2312 12-13 0.03995042009744765 2312 14-15 0.09457956120257194 2312 16-17 0.08110584894663475 2312 18-19 0.20350020148460857 2312 20-21 0.13956628164958573 2312 22-23 0.07358884822071587 2312 24-25 0.11406959977424691 2312 26-27 0.19100928843074882 2312 28-29 -0.008630194080041065 2312 30-31 0.14281691390674212 2312 32-33 0.2704914884374219 2312 34-35 0.20174405987364707 2312 36-37 -0.006504554506946647 2312 38-39 0.1908915515289209 2312 40-41 0.36053041330728064 2312 42-43 0.046549775267763494 2312 44-45 -0.10809600334286529 2312 46-47 0.15141607655089473 2312 48-49 0.18090944503936868 2312 50-51 0.10647420876122027 2312 52-53 -0.0513907784641674 2312 54-55 -0.3863863751354728 2312 56-57 -0.06952336233518963 2312 58-59 0.020424329679492814 2312 60-61 -0.01642641509993581 2312 62-63 0.02210176799373187 2312 64-65 -0.027792869365825368 2312 66-67 -0.042886112756935546 2312 68-69 0.16060990368965022 2312 70-71 0.053490956918000876 2312 72-73 -0.07138186107178868 2312 74-75 -0.30419358102285443 2312 76-77 -0.2057137116741501 2312 78-79 -0.18184599973957205 2312 80-81 -0.5789791755699092 2312 82-83 -0.5269745468280895 2312 84-85 -0.7569195777198097 2312 86-87 -0.9860891521255901 2312 88-89 -0.6324702029300333 2312 90-91 -0.547580786111471 2312 92-93 -0.3941341626828532 2312 94-95 -0.5736551402072934 2312 96-97 -0.8314374569833944 2312 98-99 -0.7046518115651317 2312 100-101 -0.7322163882960524 2313 1 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58-59 -0.25420285707053125 2313 60-61 -0.2412126740995646 2313 62-63 -0.24740747635387095 2313 64-65 -0.21468953950602554 2313 66-67 -0.0977976164830956 2313 68-69 -0.26722339806606143 2313 70-71 -0.5781786306783125 2313 72-73 -0.35593014324937755 2313 74-75 -0.29363363091549033 2313 76-77 -0.06052160134195361 2313 78-79 -0.1391453634505737 2313 80-81 -0.08362365106567538 2313 82-83 -0.21429948840949464 2313 84-85 -0.26648068830462535 2313 86-87 -0.346841725701438 2313 88-89 -0.20908006935670898 2313 90-91 -0.29814839379114844 2313 92-93 -0.1677502735834473 2313 94-95 -0.2999127188355608 2313 96-97 -0.2991667104885103 2313 98-99 -0.28467482375409503 2313 100-101 -0.3663470564784461 2314 1 -0.5206060011730642 2314 2 -0.4811626281029149 2314 3 -0.6278393177673252 2314 4 -0.1819879490868317 2314 5 -0.11552083715132255 2314 6 -0.130451284005332 2314 7 -0.3950068261933595 2314 8 -0.3540365748478891 2314 9 -0.4686320881605823 2314 10-11 -0.5010243703215522 2314 12-13 -0.8733179901427519 2314 14-15 -1.2124171202133667 2314 16-17 -1.4838475328485572 2314 18-19 -1.4277300450387784 2314 20-21 -1.4250769705627988 2314 22-23 -1.2366671568361696 2314 24-25 -1.107945649909702 2314 26-27 -1.1186165807222181 2314 28-29 -0.8286325644972337 2314 30-31 -1.0316892491779583 2314 32-33 -1.186807421170677 2314 34-35 -1.442464220783748 2314 36-37 -1.1333791594374176 2314 38-39 -1.030540970594977 2314 40-41 -0.8950084615346938 2314 42-43 -0.9172342007120093 2314 44-45 -1.0174433484756165 2314 46-47 -1.1299662717424042 2314 48-49 -1.4674786712690988 2314 50-51 -1.0013876749303137 2314 52-53 -0.9928691286538083 2314 54-55 -1.1284296747562124 2314 56-57 -1.1035408244085119 2314 58-59 -1.0043168997768888 2314 60-61 -1.0898441616233292 2314 62-63 -1.1012409153185274 2314 64-65 -1.1062161468626641 2314 66-67 -1.2787307714168605 2314 68-69 -0.9995907916327234 2314 70-71 -1.1569823363816205 2314 72-73 -0.9614656158126209 2314 74-75 -0.843720287723233 2314 76-77 -0.6999516225464646 2314 78-79 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-0.9853248351900987 2315 36-37 -1.3205088246932846 2315 38-39 -1.3182260114001423 2315 40-41 -1.4659345262154417 2315 42-43 -1.6041745218881687 2315 44-45 -1.5782476051735301 2315 46-47 -2.0778556421480303 2315 48-49 -2.7600598259642233 2315 50-51 -2.301483865772944 2315 52-53 -1.987128560419059 2315 54-55 -2.033617905047212 2315 56-57 -2.1295720914950422 2315 58-59 -2.3748769329880446 2315 60-61 -2.626190124645543 2315 62-63 -2.771571103042554 2315 64-65 -2.1738337528244713 2315 66-67 -2.0759412369805403 2315 68-69 -2.3095743885627193 2315 70-71 -1.9634007969231213 2315 72-73 -2.0067958035366438 2315 74-75 -2.0069242528110216 2315 76-77 -1.938808166561369 2315 78-79 -1.8154716739795198 2315 80-81 -2.023137295053399 2315 82-83 -1.9541724669000082 2315 84-85 -1.6933872754968604 2315 86-87 -1.8924709353880935 2315 88-89 -2.016690434214766 2315 90-91 -1.7530911653203631 2315 92-93 -1.5911368596426456 2315 94-95 -1.7817848394554154 2315 96-97 -1.5044310823511644 2315 98-99 -1.7109309264719634 2315 100-101 -2.327864475906381 2316 1 -5.678012400083887 2316 2 -5.48920437986148 2316 3 -6.070401151214114 2316 4 -8.145425661816578 2316 5 -8.697209060432467 2316 6 -9.523947993785228 2316 7 -11.682036551630166 2316 8 -11.697408483175547 2316 9 -11.698473249948648 2316 10-11 -11.60232117086614 2316 12-13 -10.39720044986592 2316 14-15 -10.092071079823075 2316 16-17 -8.97901090929512 2316 18-19 -9.480389450530048 2316 20-21 -10.850249423478616 2316 22-23 -10.353160236028366 2316 24-25 -10.534807460665313 2316 26-27 -8.077611361752403 2316 28-29 -6.462231384556233 2316 30-31 -8.572661565946735 2316 32-33 -6.224285297276868 2316 34-35 -4.626355756976174 2316 36-37 -5.46115542447032 2316 38-39 -4.584957807445441 2316 40-41 -3.9597214181945546 2316 42-43 -4.061808741215753 2316 44-45 -4.331653695650559 2316 46-47 -4.289917485720167 2316 48-49 -5.175152826022899 2316 50-51 -5.119098135561124 2316 52-53 -5.067803324251717 2316 54-55 -4.33189461689372 2316 56-57 -4.914394012518336 2316 58-59 -5.1784115219688545 2316 60-61 -5.782247156858183 2316 62-63 -5.154852215522748 2316 64-65 -5.684495021380478 2316 66-67 -5.21948638748221 2316 68-69 -5.488222513892765 2316 70-71 -4.971529647477606 2316 72-73 -3.9146923006322254 2316 74-75 -4.248788361242635 2316 76-77 -4.858700204348139 2316 78-79 -5.086828829409903 2316 80-81 -5.8512010162292505 2316 82-83 -5.277827339868381 2316 84-85 -5.345569989807721 2316 86-87 -5.60759327300471 2316 88-89 -7.373226842124172 2316 90-91 -6.628015767796452 2316 92-93 -5.9809244557231445 2316 94-95 -5.333714664016821 2316 96-97 -6.716611449657389 2316 98-99 -8.348299934275754 2316 100-101 -8.99303140661107 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6653.0 3 2687.0 4 2869.0 5 3036.0 6 3263.0 7 3917.0 8 4995.0 9 6017.0 10 7415.0 11 9075.0 12 10658.0 13 12218.0 14 13200.0 15 15489.0 16 17805.0 17 20885.0 18 17992.0 19 15451.0 20 16351.0 21 17668.0 22 19672.0 23 21836.0 24 25177.0 25 28745.0 26 33622.0 27 39571.0 28 45985.0 29 55011.0 30 65809.0 31 79902.0 32 97424.0 33 122745.0 34 159455.0 35 211734.0 36 285300.0 37 338171.0 38 217407.0 39 26181.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.49357007336261 17.537549175259613 13.893555456614703 23.07532529476307 2 16.22020777183546 19.532628713575527 38.86556923869539 25.381594275893622 3 20.551125898525218 23.41626746895436 28.5148980153759 27.517708617144525 4 13.464449478460361 15.619759014375138 35.65729948859994 35.25849201856456 5 14.816801178746802 36.87101545807634 32.641069483971485 15.671113879205365 6 33.45378854799161 34.245774775822575 16.81347496393072 15.486961712255095 7 30.13927121223413 31.979343720774807 19.386850855028598 18.494534211962467 8 27.7132811463447 32.74988318498307 19.024791703922485 20.512043964749747 9 27.124861772200585 16.69676426751286 19.507860954853598 36.67051300543295 10-11 26.244612976833725 25.423287996886714 25.86823946470254 22.463859561577017 12-13 27.497918821543514 22.70154747393961 26.451802214027815 23.348731490489065 14-15 24.045576143975968 24.19854929063961 25.076132079394142 26.679742485990282 16-17 23.459348944712797 27.393673276312956 25.503919830477184 23.643057948497063 18-19 23.380140659930188 26.132529717961482 27.588738072135506 22.898591549972828 20-21 24.49121154638581 24.96651245376316 27.334799777510543 23.207476222340485 22-23 23.88719080758462 24.880484280721753 27.55249970328292 23.679825208410715 24-25 23.951069954299278 25.127178713363733 27.560201448390863 23.361549883946118 26-27 23.5528564662846 25.15363191247492 27.878025812804424 23.415485808436053 28-29 23.204543592692985 25.988265284427392 27.53204127979174 23.275149843087878 30-31 23.487215434781877 25.203843417122414 27.368732334411305 23.940208813684404 32-33 23.308916447643437 25.230899332599787 27.500348355043457 23.959835864713316 34-35 24.201004295922537 25.28321351631146 27.138861773929392 23.376920413836615 36-37 24.127242283086815 24.899329987676 27.46940020804255 23.504027521194633 38-39 23.78687193384554 25.203225077960205 27.419264939770034 23.59063804842422 40-41 23.88273078515082 25.352908151511055 27.311911444383558 23.452449618954567 42-43 23.642977325380834 25.411798450819127 26.847735949317702 24.097488274482334 44-45 23.728783429550994 25.352264253922375 26.903125408380546 24.01582690814609 46-47 24.325792937842724 25.739632106340498 26.425063641379953 23.509511314436825 48-49 24.26074976608096 25.674305419594905 26.238689909231006 23.82625490509313 50-51 23.808986162052257 25.99369264016435 26.537058621740023 23.66026257604337 52-53 24.31737537754099 25.923412478112652 26.108792822511234 23.650419321835123 54-55 23.757156254744412 25.69787296602857 26.314045957616905 24.23092482161011 56-57 24.07360517671229 25.917073819944157 25.922214785705812 24.08710621763774 58-59 24.475110873121174 25.447402262169618 25.99801424143951 24.079472623269698 60-61 23.876804526414816 25.69379914610814 26.733897277366868 23.695499050110172 62-63 23.74535966792603 25.794685019555878 26.645529678306346 23.814425634211748 64-65 23.598193703618648 26.219259666395374 26.237756956182086 23.9447896738039 66-67 23.646793729190588 26.00250313492426 26.002262910843232 24.34844022504192 68-69 23.751576519765052 25.79837363218142 26.379661994162372 24.07038785389115 70-71 24.055403424169345 25.719298254043103 26.03322888997846 24.19206943180909 72-73 23.886749892115752 25.740965354980023 26.32635153253299 24.04593322037124 74-75 23.615616357325337 26.554669335817323 25.900604090748878 23.929110216108462 76-77 23.907899817599848 25.935686826316655 25.9169446160478 24.23946874003569 78-79 23.862446740544126 26.06497887882493 26.131894836466767 23.940679544164173 80-81 24.078772362786477 26.138945268353435 25.829282661008218 23.952999707851866 82-83 23.998515349199238 26.051417445127377 26.055693623809184 23.894373581864198 84-85 23.89419627059775 26.286013408282365 25.79522040161426 24.024569919505623 86-87 23.99226661906973 26.262025452989658 25.739099769310208 24.0066081586304 88-89 23.80156998421944 26.28600502988793 25.396009385216434 24.516415600676194 90-91 23.883743780825604 26.02741601862329 25.655526807014002 24.433313393537105 92-93 24.080256470133744 26.039771706216385 25.866168102872507 24.013803720777368 94-95 23.821786003279012 25.870494484689942 26.07737867567209 24.230340836358955 96-97 23.51265497381746 25.829394020059986 26.29142044957663 24.366530556545925 98-99 23.56287136670637 26.1624533287456 25.5909729942864 24.683702310261634 100-101 23.64836178716748 25.802804177313575 25.815442316864107 24.733391718654833 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 213.0 1 172.5 2 186.5 3 237.5 4 210.0 5 196.5 6 314.0 7 631.0 8 1023.0 9 1269.5 10 1169.5 11 1042.5 12 1134.0 13 1248.0 14 1374.5 15 1527.0 16 1657.0 17 1799.0 18 1951.0 19 2153.0 20 2483.5 21 2864.0 22 3346.0 23 3997.5 24 4890.5 25 6106.5 26 7694.0 27 9786.0 28 11784.5 29 13869.5 30 16527.5 31 19913.0 32 24153.5 33 26350.0 34 27260.5 35 29776.5 36 33570.5 37 37213.0 38 40347.0 39 42693.0 40 45376.5 41 49482.5 42 52922.0 43 56208.5 44 60082.5 45 65020.0 46 69395.5 47 77260.0 48 92934.0 49 95369.5 50 84539.0 51 79844.5 52 80322.5 53 83060.5 54 85075.5 55 82282.5 56 76248.5 57 70006.5 58 66037.0 59 60845.0 60 52177.5 61 45010.5 62 38723.5 63 30408.5 64 22204.0 65 17013.5 66 13213.5 67 10608.5 68 8329.0 69 6383.5 70 5086.0 71 3812.5 72 2936.0 73 2515.0 74 2002.5 75 1449.0 76 1055.5 77 702.5 78 483.0 79 317.5 80 202.0 81 137.5 82 95.0 83 59.0 84 33.0 85 22.5 86 16.0 87 12.5 88 10.0 89 8.5 90 5.5 91 4.5 92 6.0 93 6.0 94 3.0 95 2.0 96 1.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0765350612736914 2 0.03146921854003005 3 0.0168156129603214 4 0.021379850763837208 5 0.026184311609643324 6 0.03271837835993963 7 0.07898533630505251 8 0.05702895023971858 9 0.07201886807863364 10-11 0.061713299564379535 12-13 0.04069378336397779 14-15 0.04653120329163221 16-17 0.0579898424088798 18-19 0.05445856368721231 20-21 0.019289910295911546 22-23 0.014029025669753852 24-25 0.022532921366830674 26-27 0.025583754003917557 28-29 0.0066541782714414684 30-31 0.001056981386077345 32-33 0.009080430998573556 34-35 0.011506683725705643 36-37 0.004516193195057747 38-39 0.010041323167734779 40-41 0.004107814023164227 42-43 0.0019698289467805067 44-45 3.36312259206428E-4 46-47 4.5642378035158084E-4 48-49 7.927360395580089E-4 50-51 0.0010089367776192838 52-53 1.2011152114515286E-4 54-55 1.4413382537418343E-4 56-57 0.0037474794597287687 58-59 0.0037715017639578 60-61 0.00504468388809642 62-63 0.002666475769422393 64-65 6.245799099547948E-4 66-67 4.3240147612255025E-4 68-69 0.0035553010258965244 70-71 0.002282118901757904 72-73 0.022340742932998432 74-75 0.016215055354595633 76-77 0.025679843220833682 78-79 0.020683203941195324 80-81 0.01263573202447008 82-83 0.004876527758493205 84-85 0.003531278721667494 86-87 0.0015614497748869872 88-89 0.0019458066425514762 90-91 0.0064379775333801926 92-93 0.010617858469231512 94-95 0.013596624193631304 96-97 0.011578750638392735 98-99 0.023421746623304807 100-101 0.019049687253621245 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2081399.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.276684863947686 #Duplication Level Percentage of deduplicated Percentage of total 1 77.49587095677816 42.83714837134982 2 12.609178298616659 13.939871504119223 3 4.0934741578878615 6.788210430728429 4 1.8599763473142725 4.112533056195502 5 0.9991043071747493 2.7613586966955705 6 0.6277056598564289 2.08184927683201 7 0.4277329547983733 1.6550561823820402 8 0.30923154627447186 1.367463578672419 9 0.23433800212090514 1.1658085105395941 >10 1.2125976284698838 12.13348619355927 >50 0.08447024197244143 3.202299753002317 >100 0.0407075325183913 4.127317876058105 >500 0.003420035663813335 1.2779660592176052 >1k 0.0020169441094283736 1.9729849242252655 >5k 1.7538644429811943E-4 0.5766455864228726 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6547 0.31454805157492627 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5349 0.25699061064216905 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4341 0.20856164531644342 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4306 0.20688008402041128 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2921 0.1403383013059966 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2761 0.13265116395270682 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2280 0.1095417072843794 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.804460845806114E-5 0.0 10-11 0.0 0.0 0.0 4.804460845806114E-5 0.0 12-13 4.804460845806114E-5 0.0 0.0 4.804460845806114E-5 0.0 14-15 4.804460845806114E-5 7.206691268709171E-5 0.0 4.804460845806114E-5 2.402230422903057E-5 16-17 9.608921691612228E-5 9.608921691612228E-5 0.0 4.804460845806114E-5 4.804460845806114E-5 18-19 9.608921691612228E-5 1.2011152114515286E-4 0.0 4.804460845806114E-5 9.608921691612228E-5 20-21 9.608921691612228E-5 1.4413382537418343E-4 0.0 4.804460845806114E-5 9.608921691612228E-5 22-23 1.9217843383224456E-4 1.4413382537418343E-4 0.0 1.68156129603214E-4 1.2011152114515286E-4 24-25 1.9217843383224456E-4 1.4413382537418343E-4 0.0 3.603345634354586E-4 1.4413382537418343E-4 26-27 1.9217843383224456E-4 1.4413382537418343E-4 0.0 6.005576057257643E-4 1.9217843383224456E-4 28-29 1.9217843383224456E-4 1.4413382537418343E-4 0.0 0.0010329590818483145 1.9217843383224456E-4 30-31 1.9217843383224456E-4 1.4413382537418343E-4 0.0 0.002738542682109485 1.9217843383224456E-4 32-33 1.9217843383224456E-4 1.4413382537418343E-4 0.0 0.005525129972677031 1.9217843383224456E-4 34-35 1.9217843383224456E-4 1.4413382537418343E-4 0.0 0.008912274868970341 1.9217843383224456E-4 36-37 1.9217843383224456E-4 1.4413382537418343E-4 0.0 0.012251375156805591 1.9217843383224456E-4 38-39 2.402230422903057E-4 1.9217843383224456E-4 0.0 0.015350252402350535 1.9217843383224456E-4 40-41 2.402230422903057E-4 2.402230422903057E-4 0.0 0.01849717425635354 1.9217843383224456E-4 42-43 2.402230422903057E-4 2.402230422903057E-4 0.0 0.022172586803395216 1.9217843383224456E-4 44-45 2.402230422903057E-4 2.402230422903057E-4 0.0 0.02575191013352077 1.9217843383224456E-4 46-47 2.402230422903057E-4 2.402230422903057E-4 0.0 0.03077257171738816 1.9217843383224456E-4 48-49 2.402230422903057E-4 2.402230422903057E-4 0.0 0.0370904377296232 1.9217843383224456E-4 50-51 2.402230422903057E-4 2.402230422903057E-4 0.0 0.04285579074459054 1.9217843383224456E-4 52-53 2.402230422903057E-4 2.402230422903057E-4 0.0 0.05349767151805108 2.8826765074836685E-4 54-55 2.402230422903057E-4 2.402230422903057E-4 0.0 0.07259540338013039 2.8826765074836685E-4 56-57 2.402230422903057E-4 3.36312259206428E-4 0.0 0.10721154377416343 2.8826765074836685E-4 58-59 2.402230422903057E-4 3.36312259206428E-4 0.0 0.1426204202077545 2.8826765074836685E-4 60-61 2.402230422903057E-4 3.603345634354586E-4 0.0 0.17730862751447463 2.8826765074836685E-4 62-63 2.402230422903057E-4 3.843568676644891E-4 0.0 0.22030855208443936 4.3240147612255025E-4 64-65 2.402230422903057E-4 3.843568676644891E-4 0.0 0.27414253586169685 4.3240147612255025E-4 66-67 2.402230422903057E-4 3.843568676644891E-4 0.0 0.35625077171652336 4.3240147612255025E-4 68-69 2.402230422903057E-4 3.843568676644891E-4 0.0 0.45743271712920014 4.3240147612255025E-4 70-71 2.402230422903057E-4 3.843568676644891E-4 0.0 0.5486694285910583 4.3240147612255025E-4 72-73 2.402230422903057E-4 3.843568676644891E-4 0.0 0.6441821102056837 4.804460845806114E-4 74-75 2.402230422903057E-4 3.843568676644891E-4 0.0 0.744162940406909 4.804460845806114E-4 76-77 2.402230422903057E-4 3.843568676644891E-4 0.0 0.86828618635831 4.804460845806114E-4 78-79 2.402230422903057E-4 3.843568676644891E-4 0.0 0.965816741528174 4.804460845806114E-4 80-81 2.402230422903057E-4 3.843568676644891E-4 0.0 1.069448961972212 5.284906930386725E-4 82-83 2.402230422903057E-4 3.843568676644891E-4 0.0 1.1914582451514582 6.245799099547948E-4 84-85 2.402230422903057E-4 3.843568676644891E-4 0.0 1.3716975937818745 6.245799099547948E-4 86-87 2.402230422903057E-4 3.843568676644891E-4 0.0 1.610791587773416 6.966468226418866E-4 88-89 2.402230422903057E-4 3.843568676644891E-4 0.0 1.8839492091617225 7.446914310999476E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAGCG 120 1.7035443E-4 23.766256 5 TACATGG 10220 0.0 23.762257 2 GTACATG 10400 0.0 23.717701 1 ACATGGG 10665 0.0 22.283274 3 GAGTACT 6150 0.0 21.214201 12-13 TAAGGTG 985 0.0 20.750233 5 GTACTTT 6490 0.0 19.917583 14-15 GTATCAA 17590 0.0 19.318737 1 AGTACTT 6530 0.0 19.288218 12-13 CATGGGG 5585 0.0 19.233358 4 ATAGGAC 610 0.0 18.700417 3 GGTAAGG 925 0.0 18.498251 3 GTAAGGT 925 0.0 18.498251 4 AGAGTAC 12985 0.0 18.396137 10-11 GTATAGG 575 0.0 18.184166 1 GTACACG 450 0.0 17.954567 1 AGGTAAG 910 0.0 17.756413 2 CATGGGA 4830 0.0 17.516315 4 ATTCGCG 315 0.0 17.338129 80-81 TACTTTT 7130 0.0 17.296581 14-15 >>END_MODULE