FastQCFastQC Report
Thu 26 May 2016
SRR936500_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936500_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2081399
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT78510.37719822100423805No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65360.3140195608818876No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59350.28514475119859284No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT37450.17992705867543898No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32880.15797067261010503No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT25900.12443553590637836No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC25360.12184112704964306No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT21830.10488138026394746No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC21450.10305568514254115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG104750.022.2197572
GTACATG108150.022.011221
GAGTACT77750.021.92896812-13
GTAAGGT8850.021.4639474
ACATGGG105250.021.2095413
GTACTTT81650.020.61979114-15
AGGTAAG9050.020.4697842
AGTACTT81700.020.0258712-13
GGTAAGG9200.019.1015833
TAAGGTG9500.018.9955965
GTATCAA198750.018.6713961
ACTTTTT93950.018.27408616-17
AGAGTAC152650.018.090310-11
TACTTTT89000.018.08980414-15
GACCGCG2403.099396E-617.808377
GTCTAGA8350.017.6403881
CATGGGA48900.017.3834934
AAGGTAA10550.017.1144941
TCGCACA3101.1206794E-716.850934
CATGGGG56700.016.8347194
Produced by FastQC (version 0.11.5)