##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936500_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2081399 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.829431550606106 31.0 31.0 34.0 30.0 34.0 2 31.984855858967933 31.0 31.0 34.0 30.0 34.0 3 32.069847732222414 33.0 31.0 34.0 30.0 34.0 4 35.77115680366907 37.0 35.0 37.0 35.0 37.0 5 35.592216100805274 37.0 35.0 37.0 33.0 37.0 6 35.68415954845755 37.0 35.0 37.0 33.0 37.0 7 35.62660691198564 37.0 35.0 37.0 33.0 37.0 8 35.593048713869855 37.0 35.0 37.0 33.0 37.0 9 37.21293418513221 39.0 37.0 39.0 33.0 39.0 10-11 37.09981147295641 39.0 37.0 39.0 33.0 39.0 12-13 36.98839842817259 39.0 37.0 39.0 33.0 39.0 14-15 38.099504708131406 40.0 37.0 41.0 33.0 41.0 16-17 37.98854015976754 40.0 37.0 41.0 33.0 41.0 18-19 38.129795632649 40.0 37.0 41.0 33.0 41.0 20-21 38.14022875959871 40.0 37.0 41.0 33.0 41.0 22-23 38.0489718694013 40.0 37.0 41.0 33.0 41.0 24-25 37.89558008820029 40.0 37.0 41.0 33.0 41.0 26-27 37.6797007685696 39.5 37.0 41.0 32.0 41.0 28-29 37.59944753504735 39.0 37.0 41.0 32.0 41.0 30-31 37.57656148580834 39.0 37.0 41.0 32.0 41.0 32-33 37.41944168321403 39.0 36.0 41.0 32.0 41.0 34-35 37.23779462755579 39.0 36.0 41.0 31.0 41.0 36-37 37.02481383915338 39.0 36.0 41.0 31.0 41.0 38-39 36.83762796080905 39.0 35.0 41.0 31.0 41.0 40-41 36.63496403140388 39.0 35.0 41.0 30.0 41.0 42-43 36.33682393428651 39.0 35.0 40.0 30.0 41.0 44-45 36.05788582583157 38.5 35.0 40.0 29.5 41.0 46-47 35.680632593750644 38.0 35.0 40.0 28.5 41.0 48-49 35.27635547052728 38.0 34.0 40.0 27.0 41.0 50-51 34.961069453766434 38.0 34.0 40.0 26.0 41.0 52-53 34.5714824500252 38.0 33.5 40.0 25.5 41.0 54-55 34.35003716250464 38.0 33.0 40.0 25.0 41.0 56-57 34.003813060350275 37.0 33.0 40.0 24.0 41.0 58-59 33.5900435236108 36.5 32.5 39.5 23.0 41.0 60-61 33.128809036614314 36.0 32.0 39.0 22.0 40.5 62-63 32.780736898595606 35.5 31.5 39.0 22.0 40.0 64-65 32.32853263598186 35.0 31.0 38.5 20.5 40.0 66-67 31.881975296423224 35.0 31.0 38.0 20.0 40.0 68-69 31.497045016356786 35.0 30.5 37.0 18.5 39.5 70-71 31.109110987369554 34.0 30.0 37.0 18.0 39.0 72-73 30.63825124351458 34.0 30.0 36.0 15.5 39.0 74-75 30.15141378467079 34.0 29.0 36.0 12.0 38.5 76-77 28.691742909456572 32.5 27.0 34.5 9.0 36.0 78-79 29.470646666016464 33.5 29.0 35.0 7.5 37.0 80-81 29.274490859273016 34.0 29.0 35.0 7.0 36.5 82-83 28.99305851496998 34.0 29.0 35.0 3.5 36.0 84-85 28.627041715692187 33.0 29.0 35.0 2.0 36.0 86-87 28.254606637170482 33.0 27.5 35.0 2.0 35.0 88-89 27.788935230582894 33.0 27.0 35.0 2.0 35.0 90-91 27.309094267845808 33.0 26.0 35.0 2.0 35.0 92-93 26.689843706084226 32.0 25.0 34.0 2.0 35.0 94-95 26.124672155603033 32.0 24.5 34.0 2.0 35.0 96-97 25.36817712509711 32.0 21.5 34.0 2.0 35.0 98-99 24.44600026232356 31.5 10.0 34.0 2.0 35.0 100-101 21.218034840989162 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.40739101496000885 1101 2 0.4244245534520239 1101 3 0.36793319477462916 1101 4 0.3568427620990775 1101 5 0.3400968913107576 1101 6 0.3394946227557938 1101 7 0.42279273462109046 1101 8 0.421868456405349 1101 9 0.4438330606019747 1101 10-11 0.47515545120901237 1101 12-13 0.3855803651868115 1101 14-15 0.6912822089859745 1101 16-17 0.579377107507753 1101 18-19 0.5231081607190049 1101 20-21 0.5357937392551477 1101 22-23 0.48625810347860465 1101 24-25 0.5347490678041567 1101 26-27 0.41472975671048573 1101 28-29 0.4065674792275047 1101 30-31 0.48902830209166126 1101 32-33 0.520197573286552 1101 34-35 0.4783350186911619 1101 36-37 0.42873925808329005 1101 38-39 0.3595970122057466 1101 40-41 0.21953156031195675 1101 42-43 0.3285889271253595 1101 44-45 0.24105873030574543 1101 46-47 0.2883028455550303 1101 48-49 0.2988352229443194 1101 50-51 0.2562019327580387 1101 52-53 0.4402020069365733 1101 54-55 0.4449863154921374 1101 56-57 0.33484931876018464 1101 58-59 0.23647494870446906 1101 60-61 0.4776711854299549 1101 62-63 0.4743992058847297 1101 64-65 0.5651648607079025 1101 66-67 0.1703113271904364 1101 68-69 0.30787937938425713 1101 70-71 0.25932151927821323 1101 72-73 0.29101040469988604 1101 74-75 0.21550094806055853 1101 76-77 0.30750358624712604 1101 78-79 0.2232968931393735 1101 80-81 0.3450723633987174 1101 82-83 0.4598287086687627 1101 84-85 0.6455401679114523 1101 86-87 0.4948896439122308 1101 88-89 0.45826226177260665 1101 90-91 0.5734491331865037 1101 92-93 0.5746999751414776 1101 94-95 0.36010908171914835 1101 96-97 0.5264016336690673 1101 98-99 0.301141429662529 1101 100-101 1.0317875517249284 1102 1 0.37062988135677344 1102 2 0.3991411526423043 1102 3 0.33254857939001425 1102 4 0.250729401775196 1102 5 0.25453008969132185 1102 6 0.2653245822699617 1102 7 0.3061125726777689 1102 8 0.3130830313041315 1102 9 0.46608002416472516 1102 10-11 0.40965949979201355 1102 12-13 0.4542949400855889 1102 14-15 0.5503712778118839 1102 16-17 0.4926767026494545 1102 18-19 0.5431992538364057 1102 20-21 0.4392249133442192 1102 22-23 0.4216224759482472 1102 24-25 0.4752348977636771 1102 26-27 0.5246993923380145 1102 28-29 0.45791363307366595 1102 30-31 0.5555768850876177 1102 32-33 0.5888716623553805 1102 34-35 0.6289423061405586 1102 36-37 0.678243306666289 1102 38-39 0.661975554715859 1102 40-41 0.5491267020123587 1102 42-43 0.596605121457344 1102 44-45 0.6978401068239677 1102 46-47 0.7954890803728532 1102 48-49 0.9382987856973557 1102 50-51 0.903236345713502 1102 52-53 0.9855562579487156 1102 54-55 1.1368587851277638 1102 56-57 1.0066306952784032 1102 58-59 1.0156854750202555 1102 60-61 1.0734302947416907 1102 62-63 0.9717473840223789 1102 64-65 0.9034543343921158 1102 66-67 0.7734388575548046 1102 68-69 0.7843571121777764 1102 70-71 0.7513964180636385 1102 72-73 0.9541581779792381 1102 74-75 0.9505515553480102 1102 76-77 0.7514205902957123 1102 78-79 0.9939851522486869 1102 80-81 1.0146371407266521 1102 82-83 0.9304258746606671 1102 84-85 0.9530705322839204 1102 86-87 1.0102843807543387 1102 88-89 1.0671185370762544 1102 90-91 1.0257973113241512 1102 92-93 1.146076493360102 1102 94-95 0.8696333732171269 1102 96-97 1.1010878684868857 1102 98-99 1.1907062069904661 1102 100-101 1.5822328958544851 1103 1 0.2558467005348639 1103 2 0.36182445899854443 1103 3 0.2486363484714289 1103 4 0.26337264613119515 1103 5 0.30762535854064765 1103 6 0.24549136935809202 1103 7 0.18532513741271117 1103 8 0.13087459588165729 1103 9 0.2064515210102229 1103 10-11 0.2583288258220193 1103 12-13 0.31177336513433573 1103 14-15 0.3849535633439487 1103 16-17 0.3462677513656587 1103 18-19 0.2446160056053941 1103 20-21 0.24009583297399217 1103 22-23 0.18422131909491668 1103 24-25 0.2947873739383837 1103 26-27 0.247263733726804 1103 28-29 0.30988227169221716 1103 30-31 0.4237373328939995 1103 32-33 0.31249961453124087 1103 34-35 0.30771267519533296 1103 36-37 0.2576087023819724 1103 38-39 0.18920030758276596 1103 40-41 0.4650625987755177 1103 42-43 0.5781927472439534 1103 44-45 0.43266548897710067 1103 46-47 0.5365921880538522 1103 48-49 0.5127623888151334 1103 50-51 0.5538303632560186 1103 52-53 0.60908168369577 1103 54-55 0.5397076777212391 1103 56-57 0.43307857834983565 1103 58-59 0.5109693602068077 1103 60-61 0.593700203638484 1103 62-63 0.5996062164320293 1103 64-65 0.5422320801023446 1103 66-67 0.4374617181229006 1103 68-69 0.3388071224707865 1103 70-71 0.2973655450954986 1103 72-73 0.20088131827495204 1103 74-75 0.23374070248150502 1103 76-77 0.2207905674089048 1103 78-79 -0.014087794901765704 1103 80-81 0.06911313581830214 1103 82-83 0.26014748916936625 1103 84-85 0.6707938804580245 1103 86-87 0.7599859339893662 1103 88-89 0.602596364726903 1103 90-91 0.6019756588726004 1103 92-93 0.5183967794653519 1103 94-95 0.5166586435599463 1103 96-97 0.7786283744992133 1103 98-99 0.9654424738982428 1103 100-101 1.088840603097129 1104 1 0.2632783843903006 1104 2 0.25749363555324933 1104 3 0.19247071306700292 1104 4 0.23858268236955382 1104 5 0.270126159111129 1104 6 0.14042210925727971 1104 7 0.24630184296240998 1104 8 0.20815951963388812 1104 9 0.26506331745215306 1104 10-11 0.20060506517682342 1104 12-13 0.24234526401104972 1104 14-15 0.30434902030958 1104 16-17 0.39205990510578204 1104 18-19 0.31955943992372227 1104 20-21 0.3566134899742224 1104 22-23 0.2955448269988139 1104 24-25 0.17389979697608027 1104 26-27 0.26896349541967624 1104 28-29 0.350217880398219 1104 30-31 0.2938045043422619 1104 32-33 0.36453072159356026 1104 34-35 0.3701930193976253 1104 36-37 0.4006429266506828 1104 38-39 0.43909945959939733 1104 40-41 0.35346379021557084 1104 42-43 0.24405039959849262 1104 44-45 0.20617453996412394 1104 46-47 0.23024158502114744 1104 48-49 0.3757570577326348 1104 50-51 0.19710141300227946 1104 52-53 0.41472085439077944 1104 54-55 0.4445071814153394 1104 56-57 0.21867911136249774 1104 58-59 0.25832437091771965 1104 60-61 0.2935926165244709 1104 62-63 0.20470248085066345 1104 64-65 0.22788222458739682 1104 66-67 0.06463867239909149 1104 68-69 0.11657090686066951 1104 70-71 0.23571325364769535 1104 72-73 0.1857326129049639 1104 74-75 0.20380366794752192 1104 76-77 0.13598721141743297 1104 78-79 0.12763205991537419 1104 80-81 0.3789016513837815 1104 82-83 0.3143563869248034 1104 84-85 0.49634049086649057 1104 86-87 0.40392683420339637 1104 88-89 0.4092172499141036 1104 90-91 0.5073331721429533 1104 92-93 0.5867315641811892 1104 94-95 0.6553891442916893 1104 96-97 0.8638888920835619 1104 98-99 0.9183077515074096 1104 100-101 1.6653753803569131 1105 1 -0.016192180475783857 1105 2 0.08038814850253218 1105 3 0.05394964368667843 1105 4 0.17550906652743947 1105 5 0.2124357981743472 1105 6 0.17066259430362152 1105 7 0.004744869768906312 1105 8 0.1924187293189803 1105 9 0.13827945344941384 1105 10-11 0.15299992748652613 1105 12-13 0.17755892726539457 1105 14-15 0.24584554717659302 1105 16-17 0.2151397837902067 1105 18-19 0.2071130756341617 1105 20-21 0.16482001799752055 1105 22-23 0.2669589111053696 1105 24-25 0.19118908855053718 1105 26-27 0.15901709401197905 1105 28-29 0.2624280833422077 1105 30-31 0.18555698889549177 1105 32-33 0.12590653127514173 1105 34-35 0.2181816437004187 1105 36-37 -0.001256419755137017 1105 38-39 0.00993414080857491 1105 40-41 0.17249520475859725 1105 42-43 0.1264821820834996 1105 44-45 0.03663654156312646 1105 46-47 0.2020460844594325 1105 48-49 0.18130626446176734 1105 50-51 0.2529031355538649 1105 52-53 0.6118703613044261 1105 54-55 0.4048911044471666 1105 56-57 0.10318849730250435 1105 58-59 0.20043627153288668 1105 60-61 0.2776774834368254 1105 62-63 0.15577908684004882 1105 64-65 0.3637259513744979 1105 66-67 0.16362543719561629 1105 68-69 0.3239338633181674 1105 70-71 0.4589778456878584 1105 72-73 0.3728368266910458 1105 74-75 0.3747186368155262 1105 76-77 0.11441459911481999 1105 78-79 0.30052947482822745 1105 80-81 0.22088962240733778 1105 82-83 0.48979301996318725 1105 84-85 0.5975523193599948 1105 86-87 0.608238454700345 1105 88-89 0.46511041806197184 1105 90-91 0.726151348603036 1105 92-93 0.6663806785424029 1105 94-95 0.8371301244715035 1105 96-97 0.9382170650207939 1105 98-99 0.70171430130668 1105 100-101 1.1182806733135102 1106 1 0.13545495112236594 1106 2 0.12837179872946436 1106 3 0.08071894952180969 1106 4 0.16676961479469554 1106 5 0.13857261970520796 1106 6 0.03440189561740681 1106 7 -0.006416437114992846 1106 8 0.12841403185543498 1106 9 0.10777119325226892 1106 10-11 0.11927325038642067 1106 12-13 0.1869088384523252 1106 14-15 0.1999220878126593 1106 16-17 0.17219451016273268 1106 18-19 0.23843030440296786 1106 20-21 0.1166477401910413 1106 22-23 0.14879449241538367 1106 24-25 0.22507244292422257 1106 26-27 0.10398055098676906 1106 28-29 -0.01680320766755017 1106 30-31 0.17101608624554387 1106 32-33 0.1269347601939259 1106 34-35 0.09402967179561017 1106 36-37 -0.11832783055547935 1106 38-39 0.12777916623377195 1106 40-41 0.26544479332429916 1106 42-43 0.2587862031887056 1106 44-45 0.2518350276236774 1106 46-47 0.3235674811730931 1106 48-49 0.07031737943522387 1106 50-51 0.19123739811774243 1106 52-53 -0.039556631587672086 1106 54-55 -0.002682699112696696 1106 56-57 -0.20827077088836887 1106 58-59 -0.014912633493892713 1106 60-61 0.00767598803074776 1106 62-63 0.008793955041191737 1106 64-65 -0.0565286102178888 1106 66-67 0.09571006619986733 1106 68-69 -0.02482239142085163 1106 70-71 0.16005286917846817 1106 72-73 0.3370264626266426 1106 74-75 0.20245400571639394 1106 76-77 0.001468071629982859 1106 78-79 0.09623460710139753 1106 80-81 0.21509563754102246 1106 82-83 0.016291901043711476 1106 84-85 0.30540618766368866 1106 86-87 0.42311563706544675 1106 88-89 0.24706912333456188 1106 90-91 0.45397569526319614 1106 92-93 0.3661715412819113 1106 94-95 0.4219600533222341 1106 96-97 0.5698064313441193 1106 98-99 0.6131125894290506 1106 100-101 0.8506319720879567 1107 1 0.06993893790087924 1107 2 0.09381649379333723 1107 3 0.02743030266697133 1107 4 0.09732175249058628 1107 5 0.14506988237151575 1107 6 0.05422094810595013 1107 7 0.039904451242342986 1107 8 0.1417647229572836 1107 9 0.0706192358493567 1107 10-11 0.0868670531020328 1107 12-13 -0.11129711461623515 1107 14-15 0.0620104942768549 1107 16-17 0.15652204751444998 1107 18-19 -0.030877450447881927 1107 20-21 0.09011254034506777 1107 22-23 0.08649927179448724 1107 24-25 0.1232977555999426 1107 26-27 0.18694401169398844 1107 28-29 0.16263803709356495 1107 30-31 0.08604536791698791 1107 32-33 0.12836717986430557 1107 34-35 0.2037473232946354 1107 36-37 0.08337164968900623 1107 38-39 0.04938858924096934 1107 40-41 -0.009652674116075843 1107 42-43 0.023968725394873047 1107 44-45 -0.04867070226750769 1107 46-47 -0.1021412483309021 1107 48-49 -0.07879898519182404 1107 50-51 -0.2062898211498876 1107 52-53 -0.07478293498225241 1107 54-55 -0.271573510503373 1107 56-57 -0.44084574314526037 1107 58-59 -0.2085664012644557 1107 60-61 -0.10356811855206871 1107 62-63 0.007344853382214467 1107 64-65 -0.23724012997042365 1107 66-67 -0.0521326232852104 1107 68-69 -0.08442522408627084 1107 70-71 0.05067555149772929 1107 72-73 0.1451726495490675 1107 74-75 0.1748854788698715 1107 76-77 -0.030267339705357443 1107 78-79 0.04431940855598171 1107 80-81 0.2667113040746578 1107 82-83 0.27204368070375295 1107 84-85 0.3488801937253072 1107 86-87 0.42229917591663124 1107 88-89 0.4502563819504388 1107 90-91 0.45791827607095925 1107 92-93 0.6715024035558379 1107 94-95 0.7108048606760811 1107 96-97 0.7317123559789316 1107 98-99 0.7487342878297447 1107 100-101 1.1367839664675081 1108 1 0.06880074553937732 1108 2 0.191019096271976 1108 3 0.02521049909469042 1108 4 0.08855040264234049 1108 5 0.1010539853992185 1108 6 0.07949216677917548 1108 7 0.1159425246350878 1108 8 0.01437109658021285 1108 9 0.1231640525709281 1108 10-11 0.10136772126821114 1108 12-13 0.021451377294773977 1108 14-15 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-1.2163350965612558 2316 68-69 -1.7880855328964422 2316 70-71 -1.691211544149585 2316 72-73 -1.2236724562987646 2316 74-75 -1.3722520883766904 2316 76-77 -1.2585018118635354 2316 78-79 -1.4030054010576585 2316 80-81 -1.1278628592733462 2316 82-83 -1.3665478095498607 2316 84-85 -1.312084663397588 2316 86-87 -1.54941534934013 2316 88-89 -1.5503409770937857 2316 90-91 -1.497846413372205 2316 92-93 -1.7416967859921222 2316 94-95 -1.7782512421674888 2316 96-97 -1.7958318346170294 2316 98-99 -2.467826181673505 2316 100-101 -3.1190086695976085 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 13.0 4 39.0 5 70.0 6 198.0 7 431.0 8 981.0 9 1817.0 10 2947.0 11 4620.0 12 6416.0 13 8482.0 14 10707.0 15 14155.0 16 17576.0 17 21347.0 18 19777.0 19 14811.0 20 14007.0 21 14723.0 22 15868.0 23 17929.0 24 20992.0 25 25244.0 26 30524.0 27 37813.0 28 46184.0 29 57149.0 30 69130.0 31 85296.0 32 105491.0 33 133356.0 34 172293.0 35 229989.0 36 316560.0 37 371269.0 38 184701.0 39 8487.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.9083486710269 18.41883196455431 14.757584349305358 22.915235015113435 2 16.54919036734388 20.35717151603769 37.757005932837416 25.336632183781017 3 20.571285247659766 23.446835050129685 29.086124268571716 26.89575543363883 4 13.862839369097419 16.243834075062015 35.26940293523731 34.62392362060326 5 14.706701964609167 36.93013601879567 32.780914127024545 15.58224788957061 6 33.457688794892285 34.720829595863165 16.381289699860528 15.440191909384025 7 29.98382338033217 32.154622924292745 19.299903574470825 18.561650120904257 8 27.93846830905559 32.86039822254166 18.716834206223794 20.484299262178947 9 26.890182997109157 16.491071630187196 19.366205134142948 37.2525402385607 10-11 26.295751078961793 25.536454086890597 25.70958283346922 22.45821200067839 12-13 27.549111105505848 22.759350478228964 26.462667151922226 23.228871264342963 14-15 23.87190414887711 24.274662972348587 25.235605263682594 26.61782761509171 16-17 23.299953540863623 27.452929495978427 25.831447982823093 23.41566898033486 18-19 23.242221047247977 26.003383829733263 28.10094507486537 22.653450048153392 20-21 24.229880959873622 24.65221709052421 28.047745770993455 23.070156178608713 22-23 23.692249151772028 24.602118383893902 28.215098698086578 23.49053376624749 24-25 23.880476544862375 24.901184251553882 28.227528695843517 22.990810507740225 26-27 23.433094835886468 24.95875301004534 28.436544496781917 23.17160765728628 28-29 23.078076184367465 25.679630717340935 28.234412329601348 23.00788076869025 30-31 23.326948915125353 24.944537719035473 28.077691154790152 23.650822211049025 32-33 23.218994445618957 25.029069208740946 28.148699813572676 23.60323653206742 34-35 24.024392734068993 24.98076375634652 27.818695250575647 23.176148259008844 36-37 23.966353100742513 24.690632010821663 28.152997493730723 23.1900173947051 38-39 23.607493707278802 24.929193373797006 28.09143291471543 23.371880004208762 40-41 23.66405703866878 25.034916486298854 27.92682311622734 23.374203358805033 42-43 23.587989744811864 25.204777041908667 27.353374448898947 23.85385876438052 44-45 23.719007410763467 25.026539080492626 27.428376913318164 23.82607659542574 46-47 24.328485383964747 25.5393556870859 26.854915368078775 23.277243560870577 48-49 24.315865839655228 25.502789001580673 26.6093812308121 23.571963927951995 50-51 23.875525789194267 25.780233929648144 26.846754220126407 23.497486061031182 52-53 24.381778658490738 25.84495635017773 26.310221344391945 23.46304364693959 54-55 23.80278601022839 25.629280997769012 26.540991037846105 24.026941954156495 56-57 24.14103203253107 25.73231543604005 26.226311964297512 23.900340567131366 58-59 24.560739974308316 25.34182289846979 26.228873203900942 23.868563923320945 60-61 23.9476887427816 25.602304121112923 26.937260417873038 23.51274671823244 62-63 23.87396629683198 25.65042213049767 26.82811096001653 23.647500612653825 64-65 23.767824414678753 25.987965366550792 26.538303330043238 23.705906888727213 66-67 23.768411458639875 25.849203868745548 26.278012815261658 24.10437185735292 68-69 23.890977244926688 25.60649606847758 26.570711190929874 23.931815495665862 70-71 24.127058863095588 25.573010441302056 26.311216382262398 23.98871431333996 72-73 23.986648393687414 25.571160722543386 26.558550204850345 23.88364067891885 74-75 23.754346921577163 26.388708718058112 25.95848479694507 23.898459563419653 76-77 24.024464949347657 25.76675178790638 26.123636398578803 24.08514686416716 78-79 23.945296668083323 25.888632438324333 26.29472232105726 23.87134857253508 80-81 24.231088784528023 25.851394527798057 25.972680796549014 23.94483589112491 82-83 24.121857116564975 25.872088134084827 26.170717021678406 23.835337727671792 84-85 23.878157839342474 26.18246863907373 25.938809308459255 24.00056421312454 86-87 24.009570529802676 26.10801898249575 25.748127084604143 24.134283403097427 88-89 23.829290941652477 26.113416074911537 25.499222795686105 24.558070187749877 90-91 23.922943368365193 25.854910997542767 25.772907221331366 24.44923841276067 92-93 24.140136801469662 25.882367076334162 25.9166575553796 24.06083856681657 94-95 23.820359180711367 25.649472595259066 26.152402488095994 24.377765735933572 96-97 23.5184688465299 25.61457068500236 26.36104453462314 24.5059159338446 98-99 23.570060091234073 25.975247728221586 25.65660867774048 24.798083502803863 100-101 23.388758621767174 25.750536926222072 25.821590263096855 25.039114188913903 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 331.0 1 255.5 2 249.0 3 304.0 4 257.0 5 243.0 6 376.0 7 721.5 8 1144.0 9 1423.0 10 1378.5 11 1212.0 12 1242.5 13 1382.5 14 1545.0 15 1736.0 16 1932.5 17 2146.5 18 2395.0 19 2642.0 20 2966.5 21 3397.5 22 3949.5 23 4611.5 24 5485.5 25 6678.0 26 8247.5 27 10276.0 28 12408.5 29 14608.5 30 17092.0 31 20450.5 32 24625.0 33 26565.5 34 27314.0 35 29554.0 36 33181.5 37 36869.0 38 40174.0 39 42706.0 40 45117.0 41 49012.0 42 52360.0 43 55537.0 44 59137.0 45 63457.0 46 67783.5 47 76845.5 48 94373.5 49 96244.5 50 83905.5 51 79201.5 52 79611.5 53 82457.5 54 84926.0 55 82324.5 56 76087.5 57 70001.5 58 66072.5 59 60735.5 60 52186.5 61 45014.5 62 38357.5 63 29795.0 64 21702.5 65 16698.0 66 12955.5 67 10339.0 68 8202.0 69 6353.0 70 5043.5 71 3647.5 72 2840.5 73 2461.5 74 1927.0 75 1471.5 76 1119.5 77 722.5 78 459.0 79 326.0 80 230.5 81 157.5 82 108.5 83 75.5 84 41.5 85 26.0 86 14.5 87 7.5 88 4.5 89 3.5 90 2.5 91 1.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022532921366830674 2 0.012972044283676508 3 0.009464787866238045 4 0.0 5 0.0033150779836062187 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0010329590818483145 14-15 2.1620073806127512E-4 16-17 0.0 18-19 0.0014173159495128036 20-21 0.0 22-23 4.804460845806114E-5 24-25 0.0 26-27 0.00168156129603214 28-29 0.003194966462461066 30-31 0.0014173159495128036 32-33 0.0076390927448317224 34-35 0.0019938512510095374 36-37 8.888252564741311E-4 38-39 0.0012491598199095896 40-41 1.9217843383224456E-4 42-43 0.005332951538844786 44-45 0.0016575389918031093 46-47 1.68156129603214E-4 48-49 4.3240147612255025E-4 50-51 1.9217843383224456E-4 52-53 2.1620073806127512E-4 54-55 0.0017055836002611707 56-57 0.014965895534686045 58-59 0.02738542682109485 60-61 0.020755270853882415 62-63 0.013884891844379669 64-65 0.019458066425514762 66-67 0.00252234194404821 68-69 8.167583437870393E-4 70-71 7.206691268709171E-5 72-73 7.206691268709171E-5 74-75 0.0019698289467805067 76-77 0.002594408856735302 78-79 0.01006534547196381 80-81 0.01787259434639874 82-83 0.018641308081727724 84-85 0.030027880286288215 86-87 0.010497746948086358 88-89 0.04155858631622289 90-91 0.04838092071726757 92-93 0.031060839368136528 94-95 0.03322284674874928 96-97 0.05212840017699634 98-99 0.055035098988709036 100-101 0.06108871965442474 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2081399.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.257585754298894 #Duplication Level Percentage of deduplicated Percentage of total 1 75.40015119381003 39.40229866897631 2 13.691047409876056 14.309221681755396 3 4.465447256919137 7.000604788792518 4 2.0400524801234887 4.264328696932935 5 1.102501238593973 2.880702651002264 6 0.707231478958013 2.2174925795872804 7 0.4668639675234415 1.707802867291392 8 0.3427361019919315 1.4328448992749994 9 0.2567513025079621 1.207548288750399 >10 1.3763619536760896 13.137052084378537 >50 0.09552817738516191 3.394448902571613 >100 0.04886410039752533 4.642393451948693 >500 0.0040626697490582 1.4795267741982732 >1k 0.0021236682779167816 1.9431726266351221 >5k 2.770002101630584E-4 0.980561037904225 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7851 0.37719822100423805 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6536 0.3140195608818876 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5935 0.28514475119859284 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3745 0.17992705867543898 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3288 0.15797067261010503 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2590 0.12443553590637836 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2536 0.12184112704964306 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2183 0.10488138026394746 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2145 0.10305568514254115 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 4.804460845806114E-5 0.0 0.0 0.0 6 0.0 4.804460845806114E-5 0.0 0.0 0.0 7 0.0 4.804460845806114E-5 0.0 0.0 0.0 8 0.0 4.804460845806114E-5 0.0 0.0 0.0 9 0.0 4.804460845806114E-5 0.0 4.804460845806114E-5 0.0 10-11 0.0 4.804460845806114E-5 0.0 4.804460845806114E-5 0.0 12-13 4.804460845806114E-5 4.804460845806114E-5 0.0 4.804460845806114E-5 9.608921691612228E-5 14-15 4.804460845806114E-5 1.4413382537418343E-4 0.0 4.804460845806114E-5 1.2011152114515286E-4 16-17 4.804460845806114E-5 1.4413382537418343E-4 0.0 4.804460845806114E-5 1.4413382537418343E-4 18-19 4.804460845806114E-5 2.1620073806127512E-4 0.0 4.804460845806114E-5 1.9217843383224456E-4 20-21 4.804460845806114E-5 3.36312259206428E-4 0.0 4.804460845806114E-5 1.9217843383224456E-4 22-23 4.804460845806114E-5 3.36312259206428E-4 0.0 1.68156129603214E-4 1.9217843383224456E-4 24-25 4.804460845806114E-5 3.36312259206428E-4 0.0 3.603345634354586E-4 1.9217843383224456E-4 26-27 4.804460845806114E-5 3.36312259206428E-4 0.0 6.005576057257643E-4 1.9217843383224456E-4 28-29 4.804460845806114E-5 3.36312259206428E-4 0.0 9.368698649321923E-4 1.9217843383224456E-4 30-31 4.804460845806114E-5 3.36312259206428E-4 0.0 0.002618431160964332 1.9217843383224456E-4 32-33 4.804460845806114E-5 3.36312259206428E-4 0.0 0.0054770853642189705 2.402230422903057E-4 34-35 7.206691268709171E-5 3.603345634354586E-4 0.0 0.008888252564741312 2.402230422903057E-4 36-37 9.608921691612228E-5 3.843568676644891E-4 0.0 0.01215528593988947 2.402230422903057E-4 38-39 9.608921691612228E-5 3.843568676644891E-4 0.0 0.015013940143144108 2.402230422903057E-4 40-41 9.608921691612228E-5 3.843568676644891E-4 0.0 0.01813683969291808 2.402230422903057E-4 42-43 9.608921691612228E-5 3.843568676644891E-4 0.0 0.02186029684841782 2.402230422903057E-4 44-45 9.608921691612228E-5 4.0837917189351966E-4 0.0 0.025463642482772404 2.402230422903057E-4 46-47 9.608921691612228E-5 4.3240147612255025E-4 0.0 0.03034017024126561 2.402230422903057E-4 48-49 9.608921691612228E-5 4.3240147612255025E-4 0.0 0.036682058557729685 2.402230422903057E-4 50-51 9.608921691612228E-5 4.3240147612255025E-4 0.0 0.04230327774732283 2.402230422903057E-4 52-53 9.608921691612228E-5 4.3240147612255025E-4 0.0 0.052921136216554344 3.122899549773974E-4 54-55 9.608921691612228E-5 4.3240147612255025E-4 0.0 0.07211495729554977 3.36312259206428E-4 56-57 1.2011152114515286E-4 5.284906930386725E-4 0.0 0.1066350084726667 3.36312259206428E-4 58-59 1.4413382537418343E-4 5.284906930386725E-4 0.0 0.14201986260202873 3.36312259206428E-4 60-61 1.4413382537418343E-4 5.284906930386725E-4 0.0 0.1772125382975585 3.36312259206428E-4 62-63 1.4413382537418343E-4 5.765353014967337E-4 0.0 0.2205247528225006 3.36312259206428E-4 64-65 1.4413382537418343E-4 5.765353014967337E-4 0.0 0.27423862507861296 3.36312259206428E-4 66-67 1.4413382537418343E-4 5.765353014967337E-4 0.0 0.35632283862921044 3.36312259206428E-4 68-69 1.4413382537418343E-4 5.765353014967337E-4 0.0 0.45728858330382594 3.843568676644891E-4 70-71 1.4413382537418343E-4 6.005576057257643E-4 0.0 0.547708536421897 3.843568676644891E-4 72-73 1.4413382537418343E-4 6.245799099547948E-4 0.0 0.6423804373885065 3.843568676644891E-4 74-75 1.4413382537418343E-4 6.245799099547948E-4 0.0 0.7423612675897318 4.0837917189351966E-4 76-77 1.4413382537418343E-4 6.245799099547948E-4 0.0 0.8653794875465972 4.3240147612255025E-4 78-79 1.4413382537418343E-4 6.72624518412856E-4 0.0 0.961901105938842 4.804460845806114E-4 80-81 1.4413382537418343E-4 6.72624518412856E-4 0.0 1.0643802557798865 4.804460845806114E-4 82-83 1.4413382537418343E-4 6.72624518412856E-4 0.0 1.185068312226536 4.804460845806114E-4 84-85 1.4413382537418343E-4 6.72624518412856E-4 0.0 1.364250679470875 5.284906930386725E-4 86-87 1.4413382537418343E-4 6.72624518412856E-4 0.0 1.6001737293041844 5.525129972677031E-4 88-89 1.4413382537418343E-4 6.72624518412856E-4 0.0 1.8700402950131139 6.005576057257643E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 10475 0.0 22.219757 2 GTACATG 10815 0.0 22.01122 1 GAGTACT 7775 0.0 21.928968 12-13 GTAAGGT 885 0.0 21.463947 4 ACATGGG 10525 0.0 21.209541 3 GTACTTT 8165 0.0 20.619791 14-15 AGGTAAG 905 0.0 20.469784 2 AGTACTT 8170 0.0 20.02587 12-13 GGTAAGG 920 0.0 19.101583 3 TAAGGTG 950 0.0 18.995596 5 GTATCAA 19875 0.0 18.671396 1 ACTTTTT 9395 0.0 18.274086 16-17 AGAGTAC 15265 0.0 18.0903 10-11 TACTTTT 8900 0.0 18.089804 14-15 GACCGCG 240 3.099396E-6 17.80837 7 GTCTAGA 835 0.0 17.640388 1 CATGGGA 4890 0.0 17.383493 4 AAGGTAA 1055 0.0 17.114494 1 TCGCACA 310 1.1206794E-7 16.85093 4 CATGGGG 5670 0.0 16.834719 4 >>END_MODULE