FastQCFastQC Report
Thu 26 May 2016
SRR936499_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936499_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1098791
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT94090.8563047931772285No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44180.40207828422329633No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT43480.3957076459490476No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35860.3263586978779404No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT35700.32490255198668355No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC30750.2798530384759249No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT28730.26146919659880724No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC23970.2181488563339161No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC23880.21732977427008413No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT20470.1862956649626726No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG20450.1861136467262655No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20360.18529456466243355No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA18340.16691072278531585No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC16890.1537144006458007No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA15020.13669569554173633No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA14870.13533055876868302No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA11870.10802782330761719No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC10990.10001902090570455No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGC5850.025.9846231
TAAGGTG5150.024.9056935
GTACATG52750.023.8641381
TACATGG53650.022.9346392
GTATAGC4600.022.7188951
GAGTACT43550.021.92652312-13
ACATGGG53900.021.8577273
AGCGTCG1100.00287542221.5933195
GTACTTT46550.020.46193314-15
GTATAGA3052.0736479E-1020.2472421
AGTACTT44900.019.83886312-13
TCGCGGA600.00476177919.79387782-83
CATGGGG21900.019.5227284
GTCCTAT3203.965397E-1019.2981511
GAGTACG1759.9312165E-519.0012571
CGATCGT757.7443547E-418.9926124-25
GTCTTAG4400.018.3534871
ATTCGCG650.007545517818.27751480-81
ACTTTTT54700.017.98142216-17
ACCGTGG3208.252755E-917.81538
Produced by FastQC (version 0.11.5)