##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936499_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1098791 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.545472250864815 31.0 31.0 34.0 30.0 34.0 2 31.60200711509286 33.0 31.0 34.0 30.0 34.0 3 31.62670789986449 33.0 31.0 34.0 28.0 34.0 4 35.24950240764622 37.0 35.0 37.0 33.0 37.0 5 35.26538349877274 37.0 35.0 37.0 33.0 37.0 6 35.2436523415281 37.0 35.0 37.0 32.0 37.0 7 35.17010969329017 37.0 35.0 37.0 32.0 37.0 8 35.14632264006531 37.0 35.0 37.0 32.0 37.0 9 36.664005256686664 39.0 37.0 39.0 32.0 39.0 10-11 36.5390542878491 39.0 37.0 39.0 32.0 39.0 12-13 36.471548274421615 39.0 37.0 39.0 32.0 39.0 14-15 37.586406787095996 40.0 37.0 41.0 32.0 41.0 16-17 37.525065276290036 40.0 37.0 41.0 32.0 41.0 18-19 37.52021631047215 40.0 37.0 41.0 32.0 41.0 20-21 37.394782538262504 40.0 37.0 41.0 32.0 41.0 22-23 37.484815128627744 40.0 37.0 41.0 32.0 41.0 24-25 37.529614822109025 40.0 37.0 41.0 32.0 41.0 26-27 37.43399791225083 40.0 37.0 41.0 32.0 41.0 28-29 37.31350866543319 40.0 37.0 41.0 31.5 41.0 30-31 37.12254696298022 39.0 36.5 41.0 31.0 41.0 32-33 36.935270219723314 39.0 36.0 41.0 30.5 41.0 34-35 36.72656492453979 39.0 35.5 41.0 30.0 41.0 36-37 36.52464435911834 39.0 35.0 41.0 30.0 41.0 38-39 36.32442475411611 39.0 35.0 40.0 30.0 41.0 40-41 36.14097266905171 39.0 35.0 40.0 30.0 41.0 42-43 35.91201693497672 38.5 35.0 40.0 29.0 41.0 44-45 35.6270314372797 38.0 35.0 40.0 27.5 41.0 46-47 35.426173858358865 38.0 35.0 40.0 27.0 41.0 48-49 35.24455515198068 39.0 35.0 40.0 26.0 41.0 50-51 34.24828834600939 38.0 34.0 39.5 25.0 40.5 52-53 34.361682066926285 38.0 33.5 39.5 24.5 40.5 54-55 34.82611479344115 38.0 34.0 40.0 25.5 41.0 56-57 34.70685462476486 38.0 34.0 40.0 24.5 41.0 58-59 34.49091911018565 38.0 34.0 40.0 24.0 41.0 60-61 34.16569347582934 38.0 34.0 40.0 23.0 41.0 62-63 33.8401356581916 37.0 33.0 40.0 22.0 41.0 64-65 33.497288383323124 37.0 33.0 40.0 22.0 41.0 66-67 33.00314436503393 36.0 32.0 39.0 20.0 41.0 68-69 32.632922912546604 36.0 32.0 39.0 19.5 40.5 70-71 32.22017062389481 35.0 31.5 38.5 18.0 40.0 72-73 31.82354196566954 35.0 31.0 38.0 16.5 40.0 74-75 31.40609360651844 35.0 31.0 37.0 13.0 39.0 76-77 30.945580187679006 35.0 31.0 36.5 8.0 39.0 78-79 30.44477794230204 34.5 30.0 36.0 7.0 39.0 80-81 29.964068690042055 34.0 30.0 36.0 3.5 37.5 82-83 29.53799994721471 34.0 29.0 35.0 2.0 37.0 84-85 29.189373593340314 34.0 29.0 35.0 2.0 37.0 86-87 28.87617117358988 34.0 29.0 35.0 2.0 36.0 88-89 28.441110274838437 34.0 29.0 35.0 2.0 36.0 90-91 28.005917412865593 34.0 27.5 35.0 2.0 35.5 92-93 27.560950626643283 34.0 26.5 35.0 2.0 35.0 94-95 27.053827798007084 33.0 25.5 35.0 2.0 35.0 96-97 26.48923407636211 33.0 25.0 35.0 2.0 35.0 98-99 25.810836637722733 33.0 23.5 35.0 2.0 35.0 100-101 23.093768969713075 29.5 8.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.44716569255702865 1101 2 0.49739054270700933 1101 3 0.5706415410094756 1101 4 0.6150810343430493 1101 5 0.5696023589974573 1101 6 0.5682840312741462 1101 7 0.5763124532123811 1101 8 0.5604531818973726 1101 9 0.7118104174285662 1101 10-11 0.7575089559502786 1101 12-13 0.8885167422264857 1101 14-15 0.8765537718837848 1101 16-17 0.9604135659579995 1101 18-19 0.9031006861974973 1101 20-21 0.7840083845486419 1101 22-23 0.7671236014654923 1101 24-25 0.7465059371276084 1101 26-27 0.7066584738587736 1101 28-29 0.7044410759753532 1101 30-31 0.6485410410298371 1101 32-33 0.5632806908591661 1101 34-35 0.5418141740208355 1101 36-37 0.5797421677541692 1101 38-39 0.6604521192829367 1101 40-41 0.4749759243329663 1101 42-43 0.8396861813100784 1101 44-45 0.6709335114255452 1101 46-47 0.8814862554903939 1101 48-49 0.9703073006926388 1101 50-51 1.0449200291938965 1101 52-53 1.1732351239085403 1101 54-55 1.0972125547139342 1101 56-57 1.0493825070928224 1101 58-59 1.1765979202780912 1101 60-61 1.092879234455566 1101 62-63 1.1763807566193734 1101 64-65 1.0052366561418111 1101 66-67 1.0638377044009815 1101 68-69 1.0283535692664287 1101 70-71 0.9726792325226867 1101 72-73 0.9963814584932429 1101 74-75 0.9071527199082716 1101 76-77 0.9981717545996318 1101 78-79 0.899647438241292 1101 80-81 0.9056747212557283 1101 82-83 1.0047346269074104 1101 84-85 0.767390480386215 1101 86-87 0.6938464324042819 1101 88-89 0.7055911409818059 1101 90-91 0.7879452904657498 1101 92-93 0.938869508765098 1101 94-95 0.8683794903521864 1101 96-97 0.7585512688610265 1101 98-99 0.8564557359892149 1101 100-101 0.9648902731781952 1102 1 0.3780552639677879 1102 2 0.3709620603399095 1102 3 0.43374483704726075 1102 4 0.3484284478707238 1102 5 0.32530642808880117 1102 6 0.39397133136295537 1102 7 0.4647133417913878 1102 8 0.4376574532854889 1102 9 0.5121756330718341 1102 10-11 0.4603354807462665 1102 12-13 0.4763467785890043 1102 14-15 0.5042542428917258 1102 16-17 0.3913581646342905 1102 18-19 0.29037240256470653 1102 20-21 0.39842088419876376 1102 22-23 0.23476391145030107 1102 24-25 0.3267646352524878 1102 26-27 0.08860113353963328 1102 28-29 -0.0014542526232972364 1102 30-31 0.3101061818614781 1102 32-33 0.2786661552638421 1102 34-35 0.292942869117617 1102 36-37 0.09293630649158047 1102 38-39 0.28450345558668033 1102 40-41 0.17383110274853664 1102 42-43 0.18407495880143898 1102 44-45 0.2805392362968533 1102 46-47 0.3251217955973189 1102 48-49 0.5449272944837773 1102 50-51 0.5237811301972357 1102 52-53 0.6135341986870984 1102 54-55 0.5612150217166629 1102 56-57 0.5600876958140688 1102 58-59 0.39977610428926624 1102 60-61 0.6135643970690836 1102 62-63 0.5953562888888868 1102 64-65 0.6027871158262386 1102 66-67 0.5454508048343314 1102 68-69 0.3265238967836197 1102 70-71 0.36349082425473256 1102 72-73 0.15905271097742357 1102 74-75 0.39380892609321094 1102 76-77 0.4764744719923186 1102 78-79 0.3962068028004211 1102 80-81 0.6445375844038743 1102 82-83 0.6765528104605565 1102 84-85 0.3401309590788948 1102 86-87 0.5402904623594686 1102 88-89 0.5553086570828469 1102 90-91 0.29811197536881195 1102 92-93 0.2633598863093951 1102 94-95 0.19562266251818627 1102 96-97 0.41548807593098047 1102 98-99 0.3058083863313392 1102 100-101 0.28053493826889664 1103 1 0.3303637588812727 1103 2 0.21247584175022283 1103 3 0.38891177905230023 1103 4 0.5265868688775939 1103 5 0.5236261394182478 1103 6 0.573640490566504 1103 7 0.5422718149676342 1103 8 0.48825087994404015 1103 9 0.3616778502703397 1103 10-11 0.5287806159518951 1103 12-13 0.1676399270108817 1103 14-15 0.5119166200763203 1103 16-17 0.6956469221336405 1103 18-19 0.6892724982085596 1103 20-21 0.7472275097095817 1103 22-23 0.4862257408564403 1103 24-25 0.5213672785008967 1103 26-27 0.515737904255225 1103 28-29 0.6455166629492552 1103 30-31 0.5549979304050794 1103 32-33 0.4640576429605616 1103 34-35 0.8681374174177634 1103 36-37 0.9402213251856111 1103 38-39 0.7749445041911542 1103 40-41 0.889203679046247 1103 42-43 0.7196642553843446 1103 44-45 0.7743767286888144 1103 46-47 1.0142792601656225 1103 48-49 0.9089988969531291 1103 50-51 1.0926610102076708 1103 52-53 1.1670784557082285 1103 54-55 1.183590249957689 1103 56-57 1.099040830324114 1103 58-59 0.880614727017722 1103 60-61 1.0478744570638696 1103 62-63 1.1932553846196896 1103 64-65 1.1220952194693936 1103 66-67 1.1849332411442148 1103 68-69 1.0615958470488138 1103 70-71 1.1808871376187824 1103 72-73 0.8691811780672474 1103 74-75 0.955461173723851 1103 76-77 1.011188844655564 1103 78-79 0.9671462863235973 1103 80-81 1.038025107227977 1103 82-83 0.7991345424838592 1103 84-85 0.8197468620438535 1103 86-87 1.036290810155311 1103 88-89 1.0756492796374104 1103 90-91 1.0737727874721088 1103 92-93 0.8713504958216092 1103 94-95 0.9109789358857192 1103 96-97 0.8170727207444735 1103 98-99 0.9743806843923757 1103 100-101 1.1828260433901896 1104 1 0.33165450121552453 1104 2 0.3425574838263259 1104 3 0.3081092101602394 1104 4 0.4606960467901615 1104 5 0.512240322892545 1104 6 0.41262300782886996 1104 7 0.48958224374594295 1104 8 0.5123237491698021 1104 9 0.49892678514556366 1104 10-11 0.5836371651575121 1104 12-13 0.6055478750453887 1104 14-15 0.5182181710658895 1104 16-17 0.2904325329800699 1104 18-19 0.32795384225961044 1104 20-21 0.24831362216157515 1104 22-23 0.3625348474108847 1104 24-25 0.48815427453422444 1104 26-27 0.1676572276124162 1104 28-29 0.2557859348866316 1104 30-31 0.5022244084624603 1104 32-33 0.40089521930325844 1104 34-35 0.3946133760300583 1104 36-37 0.3652428375578367 1104 38-39 0.4220471549351643 1104 40-41 0.3278514428089139 1104 42-43 0.7522161474237166 1104 44-45 0.7067072007017501 1104 46-47 0.5560132623063652 1104 48-49 0.790785788048062 1104 50-51 0.7523982444511716 1104 52-53 0.8899623728488635 1104 54-55 0.7651221407377946 1104 56-57 0.7464058270710225 1104 58-59 0.7981751190675865 1104 60-61 0.7672271970055249 1104 62-63 0.9670291794021537 1104 64-65 0.9551329763061318 1104 66-67 0.9909755942829221 1104 68-69 0.9166339396884382 1104 70-71 0.9005756804441418 1104 72-73 0.8925562069253026 1104 74-75 0.9451457288899654 1104 76-77 0.8708809553865677 1104 78-79 0.9017486747216061 1104 80-81 0.9901994308999811 1104 82-83 0.764835426065197 1104 84-85 0.8188076633534855 1104 86-87 0.8018111996866573 1104 88-89 0.876806750202185 1104 90-91 0.9721346990300219 1104 92-93 0.7253811798101069 1104 94-95 0.6118887518237592 1104 96-97 0.873199024604105 1104 98-99 0.7676310464298624 1104 100-101 0.763214105597676 1105 1 0.3658369407106967 1105 2 0.38623496441127614 1105 3 0.2863163924524912 1105 4 0.3361467077109168 1105 5 0.33908375020438797 1105 6 0.4089747135977646 1105 7 0.4725136208257652 1105 8 0.6446872169518301 1105 9 0.48235383456847103 1105 10-11 0.44401355540910004 1105 12-13 0.316496548174257 1105 14-15 0.49143874380070685 1105 16-17 0.46523357155941625 1105 18-19 0.4254821138064031 1105 20-21 0.39177796363900086 1105 22-23 0.12651390088081627 1105 24-25 0.17897682820898098 1105 26-27 0.032149520636401974 1105 28-29 -0.0268500797911031 1105 30-31 -0.02635219551137169 1105 32-33 -0.28910801714557266 1105 34-35 -0.002978895563394701 1105 36-37 0.1922841693419528 1105 38-39 0.1219474373226177 1105 40-41 -0.08904252850331318 1105 42-43 -0.15876474440745625 1105 44-45 -0.004133955264471467 1105 46-47 0.05014610541002895 1105 48-49 0.06516521356380878 1105 50-51 0.16048419111974255 1105 52-53 0.18271411244010238 1105 54-55 0.34559491010291765 1105 56-57 0.2796053045740621 1105 58-59 0.3096659702682061 1105 60-61 0.3316445560341208 1105 62-63 0.19767681905418755 1105 64-65 0.12759633664388303 1105 66-67 0.45040736425596606 1105 68-69 0.3221084949318467 1105 70-71 0.345094637724479 1105 72-73 0.02679411798710518 1105 74-75 0.3990751713897893 1105 76-77 0.29408745977399775 1105 78-79 0.48411848419390324 1105 80-81 0.6161796594049314 1105 82-83 0.39015415067110126 1105 84-85 0.3778455195997559 1105 86-87 0.24936542113848148 1105 88-89 0.5824695880429793 1105 90-91 0.45352038022231866 1105 92-93 0.16127981190022922 1105 94-95 0.2549835258336195 1105 96-97 0.5671231750727372 1105 98-99 0.6011409022912595 1105 100-101 0.7463743666712155 1106 1 0.3320274378041397 1106 2 0.3116991670362701 1106 3 0.2607656515468513 1106 4 0.33266322837035744 1106 5 0.2627168529780022 1106 6 0.35840312198320134 1106 7 0.45977430798987484 1106 8 0.48427785325903017 1106 9 0.5575888361675467 1106 10-11 0.6355375070730815 1106 12-13 0.4802012645105549 1106 14-15 0.4128848737210049 1106 16-17 0.5400280431428754 1106 18-19 0.4040638893461761 1106 20-21 0.3057836826905884 1106 22-23 0.4262906572003047 1106 24-25 0.36508900188755433 1106 26-27 0.2855671556329895 1106 28-29 0.30542869889037405 1106 30-31 0.5407855195017603 1106 32-33 0.3114409992653435 1106 34-35 0.4054175124708692 1106 36-37 0.3967888631030476 1106 38-39 0.48421047607044443 1106 40-41 0.36737948984531243 1106 42-43 0.5718566380729371 1106 44-45 0.2844116372405594 1106 46-47 0.2013372609469144 1106 48-49 0.3169696621331113 1106 50-51 0.18249031150828898 1106 52-53 0.16506469281096514 1106 54-55 0.37434066941361266 1106 56-57 0.374256549032161 1106 58-59 0.5890717407410975 1106 60-61 0.5238018675782072 1106 62-63 0.567798850571684 1106 64-65 0.7503035425234756 1106 66-67 0.7032449419366174 1106 68-69 0.6833870039650662 1106 70-71 0.5584365830799456 1106 72-73 0.30442209117278196 1106 74-75 0.727641583249298 1106 76-77 0.5235220763404342 1106 78-79 0.5495897102155922 1106 80-81 0.7413889503554074 1106 82-83 0.6063493509319677 1106 84-85 0.6444837582322016 1106 86-87 0.6353778876676053 1106 88-89 0.7178047984626907 1106 90-91 0.5991367146363373 1106 92-93 0.5403206165808214 1106 94-95 0.6664087736905451 1106 96-97 0.701690201479451 1106 98-99 0.9000193536322918 1106 100-101 0.6751229478823753 1107 1 0.21210923601459086 1107 2 0.2584112521499158 1107 3 0.3082328042771607 1107 4 0.3570761124323667 1107 5 0.2484912403118429 1107 6 0.3706414431883829 1107 7 0.5406395837869269 1107 8 0.33055647536610877 1107 9 0.469699828200568 1107 10-11 0.3608442738028401 1107 12-13 0.14336309726303398 1107 14-15 0.1495064032275195 1107 16-17 0.3008461002572247 1107 18-19 0.21386617283266673 1107 20-21 0.25373764916307806 1107 22-23 0.29397520714606173 1107 24-25 0.20017006406412463 1107 26-27 0.27097962057761293 1107 28-29 0.31211090196634217 1107 30-31 0.18611681444319572 1107 32-33 -0.04290188188065969 1107 34-35 0.321203048139445 1107 36-37 0.42951330420955713 1107 38-39 0.3265806176608592 1107 40-41 0.4912771970763501 1107 42-43 0.31775383523719114 1107 44-45 0.46284265680218084 1107 46-47 0.39384481115427405 1107 48-49 0.4395366482478593 1107 50-51 0.3786320875720861 1107 52-53 0.5244266686446792 1107 54-55 0.675360006008006 1107 56-57 0.3967508225010974 1107 58-59 0.3876255982618133 1107 60-61 0.3958287829774392 1107 62-63 0.5056679809359466 1107 64-65 0.5837158705751833 1107 66-67 0.4282122892236515 1107 68-69 0.5071296283899542 1107 70-71 0.2794645082989078 1107 72-73 0.3513585494152842 1107 74-75 0.6223545159739245 1107 76-77 0.6111185460031798 1107 78-79 0.5162002601594722 1107 80-81 0.5228175967938036 1107 82-83 0.4729427423791641 1107 84-85 0.3837868888608682 1107 86-87 0.2854941060099456 1107 88-89 0.18419828873532396 1107 90-91 0.42506212486973993 1107 92-93 0.3927176960734151 1107 94-95 0.44553297776601397 1107 96-97 0.5620092557556227 1107 98-99 0.5944795032581318 1107 100-101 0.5642279588197461 1108 1 0.03492569288210845 1108 2 0.07433086335131733 1108 3 0.09146165864256517 1108 4 0.11231267906168796 1108 5 0.19275342139301443 1108 6 0.12740285315533129 1108 7 0.1345702698710909 1108 8 0.2520318103661694 1108 9 0.2879041807618492 1108 10-11 0.32390117249918404 1108 12-13 0.03679791483703809 1108 14-15 0.1223870167989034 1108 16-17 0.1901432590798393 1108 18-19 0.3486288995823159 1108 20-21 0.27602929970644396 1108 22-23 0.2382575773997857 1108 24-25 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-1.7893430277680622 2316 68-69 -1.8259204103766713 2316 70-71 -1.7539344300919844 2316 72-73 -1.5006871355611615 2316 74-75 -1.4703275380607828 2316 76-77 -1.4021366576707024 2316 78-79 -2.367589326528666 2316 80-81 -2.410563449308661 2316 82-83 -2.346389875262634 2316 84-85 -2.100318794922753 2316 86-87 -2.0827142795092293 2316 88-89 -2.2857575769532303 2316 90-91 -2.5206646002450697 2316 92-93 -3.257234591157456 2316 94-95 -2.953986912494617 2316 96-97 -3.038441033503041 2316 98-99 -4.2186504543967835 2316 100-101 -5.427914344514189 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2226.0 3 858.0 4 791.0 5 891.0 6 986.0 7 1174.0 8 1521.0 9 1887.0 10 2397.0 11 3099.0 12 4146.0 13 5116.0 14 5900.0 15 7372.0 16 9130.0 17 12037.0 18 9519.0 19 7059.0 20 6938.0 21 7511.0 22 8213.0 23 9313.0 24 10583.0 25 11972.0 26 14252.0 27 16914.0 28 19604.0 29 24018.0 30 28906.0 31 35923.0 32 44713.0 33 57839.0 34 76696.0 35 106028.0 36 148637.0 37 200255.0 38 167445.0 39 26915.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.207523057864705 18.83303948758885 14.11633742896958 21.843100025576867 2 15.332524477300302 21.12588754959401 39.647446778881026 23.89414119422466 3 20.692743695027477 24.843143697940228 29.97341202621114 24.49070058082116 4 12.504983679020123 16.540534832958002 38.33172823975547 32.622753248266406 5 13.149445085242299 38.25010901655686 34.355008889742614 14.245437008458229 6 30.923300925786812 36.94742583552556 17.351195272996474 14.77807796569116 7 27.941717210697615 33.699550018567194 20.732275609986967 17.62645716074822 8 26.134417749763077 33.68600536564006 19.864247667894738 20.315329216702125 9 26.04447770651925 17.83733388689195 21.159231864582082 34.958956542006725 10-11 24.933115491121445 26.62356255778562 27.230337183507785 21.21298476758515 12-13 26.895770354930765 24.308794724812927 27.333260198347542 21.46217472190877 14-15 22.915671918632835 25.968124062623037 27.115257253789903 24.00094676495422 16-17 21.534200308615283 29.29328010705946 27.452421810634025 21.72009777369123 18-19 21.32933830299814 28.429680896724403 29.488412485213217 20.752568315064245 20-21 22.62992138173636 26.554870011460135 29.256356915169953 21.558851691633553 22-23 21.823451212446827 26.8164174234077 29.3882219191162 21.971909445029265 24-25 22.536705280172413 26.895832726584345 29.423746213886297 21.143715779356942 26-27 22.082883965179086 26.534654816999087 30.236310767088504 21.146150450733323 28-29 21.211907478099743 28.101677438378726 29.68778795919324 20.998627124328294 30-31 21.51242822084159 27.083978941898184 29.459727449660733 21.943865387599494 32-33 21.52388579957589 27.31777351037979 29.525514893926847 21.632825796117476 34-35 22.749549277342098 26.90288690023854 29.11563709568062 21.231926726738752 36-37 22.309778528101493 26.607571089885646 29.77930168322283 21.30334869879003 38-39 21.780595018156333 27.396727309130952 29.71623331118776 21.10644436152495 40-41 21.89372107299151 27.558830100880204 29.31914849080138 21.22830033532691 42-43 21.476967575264315 27.82616886819456 28.466900175609528 22.2299633809316 44-45 22.03944838695732 27.201139448159434 28.59482470290846 22.164587461974786 46-47 22.64316050002935 27.903197695980666 28.135274221167105 21.318367582822876 48-49 22.49320495441074 27.761509712831113 28.09906637233983 21.64621896041832 50-51 21.509939681593444 28.240845868382554 28.74646474069456 21.502749709329443 52-53 22.324126874477525 28.627349794924093 27.496963565977197 21.551559764621185 54-55 21.5697089174189 27.793599132085706 28.079885248915566 22.55680670157983 56-57 22.271787182631687 28.110294803566678 27.659659668769756 21.958258345031886 58-59 23.146589072215992 27.600882494284175 27.457440766575893 21.79508766692394 60-61 22.05049117335072 27.888097937058276 28.798544523236252 21.262866366354753 62-63 21.83811544226658 27.972356689173385 28.537754952524747 21.651772916035288 64-65 21.520023589821065 28.707319670288566 27.998987967553074 21.773668772337295 66-67 21.80181065823416 28.065475702845244 27.61008041099357 22.522633227927027 68-69 21.563940156414958 28.02216887698358 28.083627690701007 22.33026327590045 70-71 22.25538150222163 27.940599253051047 27.716387069277193 22.087632175450132 72-73 22.01594264507361 27.426510814582322 28.516753249472266 22.0407932908718 74-75 21.33269297303203 29.06700111076716 27.73530964910683 21.86499626709398 76-77 21.840308440228327 28.026410422147976 27.600848483744983 22.53243265387872 78-79 21.81212454599437 28.217064783006258 27.757776838700043 22.213033832299338 80-81 22.29694091625848 28.217749627190962 27.46192884152724 22.023380615023317 82-83 21.994264012941713 28.041682659407215 27.969834845643305 21.994218482007767 84-85 21.683811056549864 28.284806995329802 27.7782809610568 22.253100987063537 86-87 22.043510179820046 28.197689633937923 27.531050578911415 22.22774960733062 88-89 21.732198239645925 28.095935564741577 27.078939199602374 23.092926996010117 90-91 21.882654166871536 27.516120984322445 27.637586068118786 22.963638780687234 92-93 22.326223715587375 28.058463971174934 27.7113506422689 21.90396167096879 94-95 21.826005825596216 27.987028945931186 27.760922992900056 22.426042235572545 96-97 21.067950913164076 28.144005287002877 28.194162631776816 22.593881168056235 98-99 21.549222415769677 28.66703878769888 26.876304965630403 22.907433830901038 100-101 21.54346722283825 28.01806950475076 27.310726719232775 23.127736553178217 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 165.0 1 146.0 2 154.0 3 176.5 4 165.0 5 189.0 6 316.5 7 602.5 8 939.5 9 1146.5 10 1108.0 11 1029.0 12 1108.0 13 1224.0 14 1335.0 15 1414.5 16 1493.5 17 1625.5 18 1772.5 19 1927.5 20 2144.0 21 2497.0 22 2943.5 23 3565.5 24 4342.0 25 5375.0 26 6932.5 27 8750.0 28 10437.0 29 12236.5 30 14398.0 31 16940.0 32 19819.0 33 21597.5 34 23370.5 35 25322.0 36 27207.5 37 29537.5 38 30770.0 39 31493.5 40 32228.0 41 33587.0 42 34919.0 43 36160.5 44 37547.0 45 39264.5 46 40671.0 47 46567.5 48 61728.5 49 60224.5 50 44098.0 51 36767.0 52 33543.5 53 32117.0 54 30621.0 55 28006.5 56 25323.5 57 22362.0 58 19920.5 59 17694.0 60 14791.0 61 12047.5 62 9790.5 63 7581.5 64 5563.5 65 4320.0 66 3325.0 67 2583.5 68 1946.5 69 1310.5 70 965.5 71 767.5 72 618.0 73 524.5 74 468.0 75 376.0 76 252.0 77 182.5 78 123.5 79 79.0 80 57.5 81 36.5 82 28.0 83 19.5 84 14.5 85 11.0 86 5.0 87 3.0 88 3.0 89 2.0 90 1.5 91 1.5 92 2.0 93 2.0 94 0.5 95 0.5 96 0.5 97 1.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012923294784904501 2 0.010921094184426338 3 0.015835586567418188 4 0.018656869231728327 5 0.03039704547998664 6 0.05369537974009616 7 0.007189720338080672 8 0.0291229178251369 9 0.04031703936417389 10-11 0.040271534805072125 12-13 0.033263832703398555 14-15 0.028531358556813807 16-17 0.030579063716393746 18-19 0.023616866173821957 20-21 0.05792730373656137 22-23 0.015062009062687991 24-25 0.0035493556099385594 26-27 0.0023662370732923732 28-29 2.2752279550888204E-4 30-31 0.0019566960413763858 32-33 0.0019111914822746092 34-35 0.0 36-37 5.460547092213169E-4 38-39 9.100911820355282E-5 40-41 0.0011376139775444102 42-43 0.004231923996465206 44-45 0.001228623095747963 46-47 0.0014106413321550687 48-49 0.0011376139775444102 50-51 0.0035038510508367832 52-53 0.004686969587482969 54-55 0.005915592683230933 56-57 7.735775047301988E-4 58-59 0.007917793283709094 60-61 0.0040499057600581 62-63 7.280729456284225E-4 64-65 0.0010921094184426338 66-67 0.016836686867657272 68-69 0.04500400895165686 70-71 0.06589060157937224 72-73 0.02075007895041004 74-75 0.04104511230980232 76-77 0.032854291671482565 78-79 0.07162417602619607 80-81 0.09501351940450914 82-83 0.05792730373656137 84-85 0.02088659262771537 86-87 0.020067510563883395 88-89 0.02384438896933084 90-91 0.04850786000249365 92-93 0.02753025825657473 94-95 0.017382741576878587 96-97 0.0225702613144811 98-99 0.05296730679446774 100-101 0.07358087206757245 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1098791.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.7344582235692 #Duplication Level Percentage of deduplicated Percentage of total 1 80.89828031080344 49.133132258950816 2 11.754818587801084 14.278450768928794 3 3.337790861302678 6.0815675887439555 4 1.3717929060253793 3.332603957695479 5 0.7183605424419721 2.181461917720123 6 0.4276179558488227 1.5582686925089027 7 0.262439809285252 1.1157397743265622 8 0.1910864192679621 0.9284424118497177 9 0.1468124395891675 0.8024916581035715 >10 0.7952601431383185 8.956799134073055 >50 0.06045058284015771 2.502012774592716 >100 0.0305044349245796 3.528518763330296 >500 0.0020934404449406948 0.9697452321236606 >1k 0.0025420348259994154 3.7762667631517073 >5k 1.4953146035290678E-4 0.8544983039006857 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9409 0.8563047931772285 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4418 0.40207828422329633 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4348 0.3957076459490476 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3586 0.3263586978779404 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3570 0.32490255198668355 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3075 0.2798530384759249 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2873 0.26146919659880724 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2397 0.2181488563339161 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2388 0.21732977427008413 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2047 0.1862956649626726 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2045 0.1861136467262655 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2036 0.18529456466243355 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1834 0.16691072278531585 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1689 0.1537144006458007 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1502 0.13669569554173633 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1487 0.13533055876868302 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1187 0.10802782330761719 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1099 0.10001902090570455 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 9.100911820355282E-5 0.0 0.0 0.0 0.0 7 9.100911820355282E-5 0.0 0.0 0.0 0.0 8 9.100911820355282E-5 0.0 0.0 0.0 0.0 9 9.100911820355282E-5 0.0 0.0 0.0 0.0 10-11 9.100911820355282E-5 0.0 0.0 0.0 0.0 12-13 9.100911820355282E-5 0.0 0.0 0.0 0.0 14-15 9.100911820355282E-5 0.0 0.0 0.0 0.0 16-17 9.100911820355282E-5 0.0 0.0 0.0 0.0 18-19 9.100911820355282E-5 0.0 0.0 0.0 0.0 20-21 9.100911820355282E-5 0.0 0.0 1.8201823640710563E-4 0.0 22-23 9.100911820355282E-5 0.0 0.0 2.2752279550888204E-4 0.0 24-25 1.8201823640710563E-4 0.0 0.0 5.460547092213169E-4 0.0 26-27 1.8201823640710563E-4 0.0 0.0 8.190820638319754E-4 0.0 28-29 2.2752279550888204E-4 0.0 0.0 0.0019566960413763858 0.0 30-31 2.7302735461065845E-4 0.0 0.0 0.004777978705686523 0.0 32-33 2.7302735461065845E-4 0.0 0.0 0.00837283887472686 0.0 34-35 2.7302735461065845E-4 0.0 0.0 0.011330635216342325 0.0 36-37 2.7302735461065845E-4 0.0 0.0 0.015380540976400424 0.0 38-39 4.550455910177641E-4 0.0 0.0 0.02307081146460064 0.0 40-41 4.550455910177641E-4 0.0 0.0 0.039679975536749026 0.0 42-43 4.550455910177641E-4 0.0 0.0 0.06670968364320422 0.0 44-45 4.550455910177641E-4 0.0 0.0 0.09128214555816347 0.0 46-47 4.550455910177641E-4 0.0 0.0 0.15066559518598166 0.0 48-49 4.550455910177641E-4 0.0 0.0 0.20304134271212632 0.0 50-51 4.550455910177641E-4 0.0 0.0 0.2437679231082162 0.0 52-53 4.550455910177641E-4 0.0 0.0 0.28786184087783756 0.0 54-55 4.550455910177641E-4 0.0 0.0 0.3244475063956658 0.0 56-57 5.460547092213169E-4 0.0 0.0 0.4218727674325691 0.0 58-59 5.460547092213169E-4 0.0 0.0 0.5471468186397594 0.0 60-61 6.370638274248697E-4 0.0 0.0 0.6616362893398289 0.0 62-63 6.370638274248697E-4 0.0 0.0 0.8020633587279109 0.0 64-65 6.370638274248697E-4 0.0 0.0 0.9798951756976532 0.0 66-67 9.100911820355282E-4 0.0 0.0 1.225392272051737 0.0 68-69 9.100911820355282E-4 0.0 0.0 1.5127990673385567 0.0 70-71 9.100911820355282E-4 0.0 0.0 1.7111534404632 0.0 72-73 0.0010466048593408574 0.0 0.0 1.865413895818222 0.0 74-75 0.0011831185366461866 0.0 0.0 2.0720956032584903 0.0 76-77 0.0011831185366461866 0.0 0.0 2.336295073403404 0.0 78-79 0.0011831185366461866 0.0 0.0 2.579516941802399 0.0 80-81 0.001456145891256845 0.0 0.0 2.839256965155339 0.0 82-83 0.001456145891256845 0.0 0.0 3.1757631797129755 0.0 84-85 0.001456145891256845 0.0 0.0 3.7511228249958366 0.0 86-87 0.001456145891256845 0.0 0.0 4.456306977396065 0.0 88-89 0.001456145891256845 0.0 0.0 5.167634245275034 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTAGC 585 0.0 25.984623 1 TAAGGTG 515 0.0 24.905693 5 GTACATG 5275 0.0 23.864138 1 TACATGG 5365 0.0 22.934639 2 GTATAGC 460 0.0 22.718895 1 GAGTACT 4355 0.0 21.926523 12-13 ACATGGG 5390 0.0 21.857727 3 AGCGTCG 110 0.002875422 21.593319 5 GTACTTT 4655 0.0 20.461933 14-15 GTATAGA 305 2.0736479E-10 20.247242 1 AGTACTT 4490 0.0 19.838863 12-13 TCGCGGA 60 0.004761779 19.793877 82-83 CATGGGG 2190 0.0 19.522728 4 GTCCTAT 320 3.965397E-10 19.298151 1 GAGTACG 175 9.9312165E-5 19.001257 1 CGATCGT 75 7.7443547E-4 18.99261 24-25 GTCTTAG 440 0.0 18.353487 1 ATTCGCG 65 0.0075455178 18.277514 80-81 ACTTTTT 5470 0.0 17.981422 16-17 ACCGTGG 320 8.252755E-9 17.8153 8 >>END_MODULE