FastQCFastQC Report
Thu 26 May 2016
SRR936499_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936499_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1098791
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT81990.7461837601509296No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56300.5123813354860024No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT51870.47206429612182843No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50560.460142101637163No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT40360.3673128010695392No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC36110.3286339258330292No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT35180.32017007784009877No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29630.269660017237127No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC27570.2509121388871951No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT21300.1938494217735675No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC17670.16081311186567782No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA17540.15962999332903163No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC16340.1487088991446053No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA16000.1456145891256845No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG15460.14070009674269265No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14620.13305533081359422No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA14450.13150817580413382No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA13150.11967699043767194No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT12210.11112213332653799No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC11830.10766378683480299No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA11710.10657167741636034No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGA1405.810907E-727.1400766
AGGCGAC2351.7644197E-1024.2539375
GTACATG49200.022.4062751
GAGTACT58750.022.35608112-13
GCGACAG2657.930794E-1021.5072297
ACATGGG48150.021.3081653
TACATGG49450.021.23312
GGCGACA2701.0022632E-921.1089466
GTACTTT62750.021.00669114-15
GTAGCGT1150.00372342320.6519363
GTAAGCG1401.5788828E-920.35598294-95
AGTACTT61000.020.2465912-13
GTAAGGT4050.019.9371344
TCTACGC1250.006031377218.9997833
TAAGGTG4750.018.9989175
CATGGGA28700.018.8665224
AGTACAT39600.018.7161642
ACTTTTT71600.018.60795216-17
CCACGTC2302.0597072E-618.585059
TAATACA7050.018.190454
Produced by FastQC (version 0.11.5)