##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936499_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1098791 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.923776223139797 31.0 31.0 34.0 30.0 34.0 2 32.0279798433005 33.0 31.0 34.0 30.0 34.0 3 32.14943060145196 33.0 31.0 34.0 30.0 34.0 4 35.8798634135154 37.0 35.0 37.0 35.0 37.0 5 35.73771809197564 37.0 35.0 37.0 33.0 37.0 6 35.799016373450456 37.0 35.0 37.0 33.0 37.0 7 35.72201810899434 37.0 35.0 37.0 33.0 37.0 8 35.69780149273156 37.0 35.0 37.0 33.0 37.0 9 37.30095168234905 39.0 37.0 39.0 34.0 39.0 10-11 37.20947568736912 39.0 37.0 39.0 33.0 39.0 12-13 37.08719174074051 39.0 37.0 39.0 33.0 39.0 14-15 38.161885654323704 40.0 37.0 41.0 33.0 41.0 16-17 38.15947072737218 40.0 37.5 41.0 33.0 41.0 18-19 38.33239487764279 40.0 38.0 41.0 33.5 41.0 20-21 38.34991913839848 40.0 38.0 41.0 34.0 41.0 22-23 38.249422319622205 40.0 38.0 41.0 33.5 41.0 24-25 38.12620780475996 40.0 37.0 41.0 33.0 41.0 26-27 37.92229095433071 40.0 37.0 41.0 33.0 41.0 28-29 37.935594212184114 40.0 37.0 41.0 33.0 41.0 30-31 37.80008209022462 40.0 37.0 41.0 33.0 41.0 32-33 37.581840859635726 40.0 36.5 41.0 33.0 41.0 34-35 37.350390565630775 39.0 36.0 41.0 32.0 41.0 36-37 37.1524070546628 39.0 36.0 41.0 31.5 41.0 38-39 36.93952944645524 39.0 35.5 41.0 31.0 41.0 40-41 36.72055923282954 39.0 35.0 41.0 31.0 41.0 42-43 36.4359091037331 39.0 35.0 40.0 30.0 41.0 44-45 36.09630948924773 39.0 35.0 40.0 30.0 41.0 46-47 35.667153717130915 38.5 35.0 40.0 28.5 41.0 48-49 35.20428225203884 38.0 35.0 40.0 26.5 41.0 50-51 34.89485762078503 38.0 34.0 40.0 26.0 41.0 52-53 34.641569233821535 38.0 34.0 40.0 24.5 41.0 54-55 34.32903254577076 38.0 34.0 40.0 24.0 41.0 56-57 34.001473437623716 38.0 33.0 40.0 23.0 41.0 58-59 33.62779773405498 37.0 33.0 40.0 22.5 41.0 60-61 33.22910362389208 37.0 32.0 39.5 21.5 40.5 62-63 32.94609029378654 36.0 32.0 39.0 21.0 40.0 64-65 32.65039893846965 36.0 31.5 39.0 19.5 40.0 66-67 32.19815324297342 35.5 31.0 39.0 18.0 40.0 68-69 31.737472822402076 35.0 31.0 38.0 16.0 40.0 70-71 31.208571511779766 35.0 30.5 37.5 12.0 39.5 72-73 30.649743217772986 34.0 29.5 37.0 8.0 39.0 74-75 30.13503887454484 34.0 29.0 36.0 7.0 39.0 76-77 28.725040066764286 32.5 27.0 35.0 7.0 37.0 78-79 29.513500292594316 34.0 29.0 35.0 3.5 37.5 80-81 29.325997846724263 34.0 29.0 35.0 2.0 37.0 82-83 28.910107108631216 34.0 29.0 35.0 2.0 36.5 84-85 28.462537916673874 33.5 28.0 35.0 2.0 36.0 86-87 28.058430129114633 33.0 27.0 35.0 2.0 36.0 88-89 27.568819275003165 33.0 26.5 35.0 2.0 35.0 90-91 26.98720138770703 32.5 25.5 34.5 2.0 35.0 92-93 26.423003555726247 32.0 25.0 34.0 2.0 35.0 94-95 25.705680152094438 32.0 23.5 34.0 2.0 35.0 96-97 24.92553542939467 31.0 19.5 34.0 2.0 35.0 98-99 23.803671034800978 31.0 4.5 34.0 2.0 35.0 100-101 20.16170909663439 27.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.40345085469351716 1101 2 0.5523489424277628 1101 3 0.3927624772161664 1101 4 0.29394122538113976 1101 5 0.3512439417056328 1101 6 0.3462166674456455 1101 7 0.33953233295167706 1101 8 0.41484951948925186 1101 9 0.4447457423115253 1101 10-11 0.4904999993687582 1101 12-13 0.5044306272809749 1101 14-15 0.6448321905295487 1101 16-17 0.5781189826682791 1101 18-19 0.49919338560921034 1101 20-21 0.4209872402397181 1101 22-23 0.5217662798244476 1101 24-25 0.5321166490159968 1101 26-27 0.4609267428791384 1101 28-29 0.5176276891896023 1101 30-31 0.5741517868344062 1101 32-33 0.610247141800663 1101 34-35 0.6859133459345301 1101 36-37 0.6751923215983311 1101 38-39 0.6281914393032295 1101 40-41 0.6909677568600046 1101 42-43 0.874744639663362 1101 44-45 1.0232552419377043 1101 46-47 1.0378898522062983 1101 48-49 1.2160437989570525 1101 50-51 1.187951474703162 1101 52-53 1.0564309542344077 1101 54-55 1.1063483116053376 1101 56-57 1.1122362168399675 1101 58-59 1.142991625566033 1101 60-61 1.162375967442344 1101 62-63 1.0200050249806552 1101 64-65 1.2557343261806722 1101 66-67 1.2350942035454437 1101 68-69 1.1359668474124156 1101 70-71 1.1699499019431663 1101 72-73 1.2801622915457074 1101 74-75 1.1867388231348919 1101 76-77 1.1766665815165211 1101 78-79 1.3256653424708205 1101 80-81 1.4387895906839425 1101 82-83 1.2219769915322303 1101 84-85 1.1636730974171208 1101 86-87 1.0853387838899913 1101 88-89 0.9527001451007386 1101 90-91 1.1148773971505292 1101 92-93 1.1815120442402502 1101 94-95 1.0238884414889284 1101 96-97 1.327064572620344 1101 98-99 0.9003660652317258 1101 100-101 0.9594106068748118 1102 1 0.34184035612740615 1102 2 0.4325946630241191 1102 3 0.27245601669711306 1102 4 0.20855735772139639 1102 5 0.1108317270522221 1102 6 0.13022256331497317 1102 7 0.22427025049093885 1102 8 0.14661354730696274 1102 9 0.2333526173481033 1102 10-11 0.37448550652293733 1102 12-13 0.3794082028750765 1102 14-15 0.44614559275404986 1102 16-17 0.4476471095528822 1102 18-19 0.3737501457564889 1102 20-21 0.3644761469974469 1102 22-23 0.2965121683652896 1102 24-25 0.2646984519764075 1102 26-27 0.4098754358384582 1102 28-29 0.49180733762580786 1102 30-31 0.6637815010488737 1102 32-33 0.6116746699377202 1102 34-35 0.6927734647170851 1102 36-37 0.8561652290990907 1102 38-39 0.7469353481348193 1102 40-41 0.7497486452863384 1102 42-43 0.799463205417446 1102 44-45 0.9273742077050215 1102 46-47 0.8586771810492309 1102 48-49 0.9773377476118341 1102 50-51 0.8340114906833307 1102 52-53 1.1361334952853852 1102 54-55 1.073933276247928 1102 56-57 1.0552362397414825 1102 58-59 1.0348255157456023 1102 60-61 0.9265009575819718 1102 62-63 0.7007303097865147 1102 64-65 0.759369624549322 1102 66-67 0.9163140975877369 1102 68-69 0.8405284804944237 1102 70-71 0.4494661454830329 1102 72-73 0.3981033748385414 1102 74-75 0.6200217625196522 1102 76-77 0.6976650496592356 1102 78-79 0.6784206498982925 1102 80-81 0.8868659583500502 1102 82-83 0.7652877435418404 1102 84-85 0.7903439542139097 1102 86-87 1.0542162531192503 1102 88-89 0.9764300967276363 1102 90-91 1.007887375852114 1102 92-93 0.9562843331454829 1102 94-95 0.936707599629024 1102 96-97 1.0429454414531527 1102 98-99 1.0065561236814986 1102 100-101 0.7939487086440806 1103 1 0.27070523744207975 1103 2 0.4623420762925292 1103 3 0.32411456812742045 1103 4 0.2616179611471878 1103 5 0.2332667327039033 1103 6 0.24335403014394785 1103 7 0.3506505652461769 1103 8 0.2870143820169915 1103 9 0.2568505740475189 1103 10-11 0.3562099217911765 1103 12-13 0.22423147911192842 1103 14-15 0.3425676430105469 1103 16-17 0.3828918708779838 1103 18-19 0.32227092655815426 1103 20-21 0.3557703822813352 1103 22-23 0.3373401398460345 1103 24-25 0.30570575568912517 1103 26-27 0.36535756782697604 1103 28-29 0.3130348395821656 1103 30-31 0.6221601202993696 1103 32-33 0.6121528892230046 1103 34-35 0.5781692998618624 1103 36-37 0.6224464220388342 1103 38-39 0.4302074549081496 1103 40-41 0.6442469497815964 1103 42-43 0.765759614425356 1103 44-45 0.806123881646414 1103 46-47 0.6020690600162766 1103 48-49 0.7796701534895902 1103 50-51 0.8538521567123709 1103 52-53 0.9104629014239762 1103 54-55 0.7265123998024023 1103 56-57 0.7096358571660559 1103 58-59 0.6486525742622575 1103 60-61 0.5105969057603872 1103 62-63 0.6662713496960819 1103 64-65 0.7224271847614716 1103 66-67 0.7141197231855045 1103 68-69 0.7888862319942938 1103 70-71 0.6119085417963994 1103 72-73 0.6871923671000815 1103 74-75 0.3850476298355403 1103 76-77 0.4848683363299564 1103 78-79 0.4975450738614242 1103 80-81 0.8047589676535907 1103 82-83 0.6728229578710305 1103 84-85 0.8383397806637838 1103 86-87 0.7509600145313016 1103 88-89 0.7944513557485955 1103 90-91 0.892098531403807 1103 92-93 1.0587917238071647 1103 94-95 0.930148387386609 1103 96-97 1.036500349722001 1103 98-99 1.0902716701557154 1103 100-101 0.9514241455626085 1104 1 0.22351443844109298 1104 2 0.2984177998428095 1104 3 0.24951385874383192 1104 4 0.19477219960921133 1104 5 0.08796902245981641 1104 6 0.197936690122539 1104 7 0.18621209063077515 1104 8 0.18844574180879192 1104 9 0.19215604973329192 1104 10-11 0.30108053274254587 1104 12-13 0.225238887763048 1104 14-15 0.24218881538237014 1104 16-17 0.2084527666321776 1104 18-19 0.18551520216838213 1104 20-21 0.22461836851341843 1104 22-23 0.1376948316419515 1104 24-25 0.14943148868385947 1104 26-27 0.3633952197253123 1104 28-29 0.33718116568874024 1104 30-31 0.4010391682981975 1104 32-33 0.4307196405399836 1104 34-35 0.46429539384468654 1104 36-37 0.48464131363746077 1104 38-39 0.6279638718913745 1104 40-41 0.5614243166672352 1104 42-43 0.664730168709994 1104 44-45 0.7820142839802386 1104 46-47 0.7063253615672664 1104 48-49 0.8573035826666597 1104 50-51 0.8864407771921492 1104 52-53 0.8611156182791859 1104 54-55 0.7521173696850809 1104 56-57 0.9744321717294113 1104 58-59 1.1581967218476237 1104 60-61 1.064183700311986 1104 62-63 1.046620584695141 1104 64-65 0.963465445302532 1104 66-67 1.0833349938216514 1104 68-69 1.0720257790801533 1104 70-71 0.9664691647107446 1104 72-73 1.0913836068423528 1104 74-75 0.9678387814295561 1104 76-77 0.9208296770229722 1104 78-79 0.9947642062307231 1104 80-81 1.0980827865718865 1104 82-83 1.086448159877449 1104 84-85 1.1039860962765218 1104 86-87 1.1974309361003428 1104 88-89 1.1572758777541772 1104 90-91 1.1528599644426123 1104 92-93 0.9752240280445754 1104 94-95 1.1094094111656503 1104 96-97 1.2625743942578573 1104 98-99 0.9572255182468865 1104 100-101 0.8485530309069134 1105 1 0.22269585014507953 1105 2 0.26860383733604465 1105 3 0.28212180961978817 1105 4 0.1361035463655469 1105 5 0.26726537183301247 1105 6 0.22729798715127458 1105 7 0.18831286664956082 1105 8 0.20469286071329407 1105 9 0.3299894076072647 1105 10-11 0.17410130578731042 1105 12-13 0.18152896979682254 1105 14-15 0.2433277683765951 1105 16-17 0.4069545969751971 1105 18-19 0.2589417704975361 1105 20-21 0.2510708707111391 1105 22-23 0.24715532145368968 1105 24-25 0.303345769441421 1105 26-27 0.33787611228541437 1105 28-29 0.4362831969248404 1105 30-31 0.45591410579245917 1105 32-33 0.45789925032921275 1105 34-35 0.2647172984802495 1105 36-37 0.22685816445612517 1105 38-39 0.32119918358909416 1105 40-41 0.3256111151633121 1105 42-43 0.31739433466109546 1105 44-45 0.15922640404374278 1105 46-47 0.31611386984135237 1105 48-49 0.13799918590719784 1105 50-51 0.5873805136402481 1105 52-53 0.5269956480778433 1105 54-55 0.31949673542857937 1105 56-57 0.4124213748194947 1105 58-59 0.5360779995742959 1105 60-61 0.33844394578643033 1105 62-63 0.521976557619773 1105 64-65 0.38763559503932044 1105 66-67 0.43972814654558334 1105 68-69 0.4420298756662824 1105 70-71 0.3134051807005527 1105 72-73 0.4947322379848629 1105 74-75 0.413856054152852 1105 76-77 0.3250431835111769 1105 78-79 0.241626162941035 1105 80-81 0.4256638909590258 1105 82-83 0.28397723324716395 1105 84-85 0.347239485436166 1105 86-87 0.37923844903520276 1105 88-89 0.4946016817274277 1105 90-91 0.4760230246070307 1105 92-93 0.30321442657971787 1105 94-95 0.301991601658365 1105 96-97 0.3896615028407169 1105 98-99 0.30542148959702686 1105 100-101 -9.557432224909235E-4 1106 1 -0.003244313023476053 1106 2 0.17447777990723523 1106 3 0.05876838853580324 1106 4 0.08365382322533321 1106 5 0.05384817579871992 1106 6 0.1044387121915662 1106 7 0.14051614381662603 1106 8 0.01552519592998891 1106 9 0.25454746664142647 1106 10-11 0.1374573606450511 1106 12-13 0.06098522093341785 1106 14-15 0.09259125235760735 1106 16-17 0.19920158224488205 1106 18-19 0.22569351223720702 1106 20-21 -0.03772712511244691 1106 22-23 -0.017749016120852446 1106 24-25 0.04533313986916454 1106 26-27 -0.09424777541548934 1106 28-29 0.13989428887490618 1106 30-31 0.13547163180096788 1106 32-33 0.03839396083358082 1106 34-35 0.09025957368451998 1106 36-37 0.1986673789017459 1106 38-39 0.2246008278164382 1106 40-41 0.1658429499629861 1106 42-43 0.38847330397762647 1106 44-45 0.5012281975182589 1106 46-47 0.47785893308327587 1106 48-49 0.3262920615491751 1106 50-51 0.4870760619355039 1106 52-53 0.2434761738292508 1106 54-55 0.3802208977944659 1106 56-57 0.20645556371123774 1106 58-59 0.4026209206802278 1106 60-61 0.29667354814929325 1106 62-63 0.5249191727062765 1106 64-65 0.5495295919164178 1106 66-67 0.18381156192306491 1106 68-69 0.3190373442961558 1106 70-71 0.3782305050623336 1106 72-73 0.26193955987591266 1106 74-75 0.3261451170937768 1106 76-77 0.33289590539093794 1106 78-79 0.3241056100993802 1106 80-81 0.5084772630046501 1106 82-83 0.5701587002591495 1106 84-85 0.5131186328459734 1106 86-87 0.4967173004590073 1106 88-89 0.4951237333117682 1106 90-91 0.68556167523694 1106 92-93 0.6439056040647166 1106 94-95 0.585238292196216 1106 96-97 0.6300372545636428 1106 98-99 0.8547373355087196 1106 100-101 0.3698670602415497 1107 1 0.11699080526232564 1107 2 0.14058506082933064 1107 3 3.366751333260254E-4 1107 4 0.053887113146736 1107 5 0.06259713386541677 1107 6 0.11014977248352409 1107 7 -0.0027404263405301776 1107 8 0.04114901979942687 1107 9 -0.086707372664101 1107 10-11 0.12989543300515294 1107 12-13 0.11824185859941139 1107 14-15 0.19266724668310786 1107 16-17 0.16422590886764965 1107 18-19 0.15126361032192648 1107 20-21 0.07086881291819225 1107 22-23 0.010668689197437686 1107 24-25 0.18395925997675988 1107 26-27 0.11584535865425494 1107 28-29 0.27299195072500027 1107 30-31 0.20285600181692587 1107 32-33 0.15815112591239 1107 34-35 0.3787705537159738 1107 36-37 0.4498530028943861 1107 38-39 0.38634614696916003 1107 40-41 0.46450135242255897 1107 42-43 0.5921697411048612 1107 44-45 0.6145980418822532 1107 46-47 0.6994128646761979 1107 48-49 0.5635291404041922 1107 50-51 0.6373272979567233 1107 52-53 0.7997583870315381 1107 54-55 0.6005577950319463 1107 56-57 0.7707040543729775 1107 58-59 0.6514638941820721 1107 60-61 0.6158136168020363 1107 62-63 0.6415221747868571 1107 64-65 0.4631942248792953 1107 66-67 0.585697777649429 1107 68-69 0.6931045268428697 1107 70-71 0.5802589112817707 1107 72-73 0.8662550155558897 1107 74-75 0.9048435466693974 1107 76-77 0.5923229991260257 1107 78-79 0.8570853190050158 1107 80-81 0.9004565565433964 1107 82-83 1.205814900242835 1107 84-85 1.1154784418334636 1107 86-87 1.3946516706155982 1107 88-89 1.2718670787685653 1107 90-91 1.1917375620171171 1107 92-93 0.9526017270883216 1107 94-95 1.1292828874104046 1107 96-97 1.3046139413235665 1107 98-99 1.217125254426854 1107 100-101 0.47771079040496645 1108 1 -0.07966743803290655 1108 2 0.011805619688061597 1108 3 -0.16141561044925012 1108 4 -0.049783166260198186 1108 5 -0.0677486505792757 1108 6 -0.08142977229550041 1108 7 -0.09388108094079683 1108 8 0.07460752600255205 1108 9 0.038375206659722494 1108 10-11 -0.11905444961724498 1108 12-13 -0.12049594798158836 1108 14-15 -0.2584157902619566 1108 16-17 -0.12028944443726886 1108 18-19 -0.015436704965651415 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-1.03617806974108 2316 64-65 -0.9336120016370764 2316 66-67 -1.0182862385729052 2316 68-69 -1.0674071926060478 2316 70-71 -1.1105474345165227 2316 72-73 -0.6967854806569065 2316 74-75 -0.8140238714733847 2316 76-77 -0.7740961575507797 2316 78-79 -0.9542106790864722 2316 80-81 -0.6502531708974075 2316 82-83 -1.1602165901847954 2316 84-85 -1.141603922334248 2316 86-87 -1.1505263576542148 2316 88-89 -1.1921246907787584 2316 90-91 -1.5768405974628124 2316 92-93 -1.7242188132587906 2316 94-95 -1.839003031358608 2316 96-97 -1.5444795160629674 2316 98-99 -0.9227328000849866 2316 100-101 -1.0087656215947938 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 9.0 4 15.0 5 31.0 6 65.0 7 189.0 8 446.0 9 897.0 10 1505.0 11 2409.0 12 3537.0 13 5015.0 14 6257.0 15 8687.0 16 11211.0 17 14452.0 18 12849.0 19 8252.0 20 7229.0 21 7408.0 22 7919.0 23 9143.0 24 10228.0 25 12546.0 26 15021.0 27 18276.0 28 22452.0 29 27798.0 30 34025.0 31 41542.0 32 51985.0 33 65674.0 34 86433.0 35 116976.0 36 164759.0 37 206186.0 38 112549.0 39 4813.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.498071726144154 19.209648923998614 15.225854252169253 22.066425097687983 2 15.444740475893564 21.27384952034713 39.32844047052313 23.95296953323618 3 20.446445149100228 24.537554472278753 30.65765378748439 24.35834659113663 4 12.534140044030531 17.043310644594975 38.232116805593094 32.190432505781395 5 12.860117698668835 38.00669495366502 34.63664677111377 14.496540576552372 6 30.62939175876031 37.22345741819873 16.977751000872775 15.169399822168184 7 27.241676503271332 33.85697136933728 20.83260904979764 18.068743077593748 8 26.059752214064623 33.534922199439194 19.99388407593575 20.411441510560437 9 25.52287013635896 17.333050598339447 21.31151420060776 35.83256506469383 10-11 24.900458776964864 26.30395589334095 27.24685586248886 21.54872946720532 12-13 26.66690025673672 24.168381430135486 27.771796456286957 21.39292185684084 14-15 22.783717432703924 25.40552839165167 27.864886817909824 23.945867357734585 16-17 21.10987027667574 28.86337138660063 28.169247389924486 21.857510946799145 18-19 20.747584704287533 27.665949051605942 30.881519796756123 20.704946447350398 20-21 21.85840067674842 25.727816262257306 30.810983237371147 21.602799823623126 22-23 21.245531423828524 25.864592130937474 31.098013804350995 21.79186264088301 24-25 21.93558251360252 25.9531091373157 31.108892499946535 21.00241584913525 26-27 21.524267603454707 25.7187451651362 31.725670965334597 21.0313162660745 28-29 20.656741194169218 27.189706121921496 31.308513281439836 20.845039402469446 30-31 21.07361166159213 26.237535681408534 30.935536612435627 21.753316044563707 32-33 21.072251907389557 26.327664179301447 31.05424968122954 21.545834232079457 34-35 22.236804084539408 25.845450967097523 30.716409828195427 21.20133512016764 36-37 21.878125651295857 25.52460885844275 31.464290557359085 21.132974932902307 38-39 21.42858768091705 26.35835967025941 31.240713816021497 20.972338832802045 40-41 21.381701690040863 26.537190910001 31.01124873724734 21.0698586627108 42-43 21.22149256828428 26.817201590323002 29.943696158569967 22.017609682822755 44-45 21.578277467500527 26.4180565434219 29.78465954674796 22.219006442329615 46-47 22.256643893151654 26.88099920731058 29.478080908926263 21.3842759906115 48-49 22.164192450527434 26.95984721034652 29.179884151804913 21.696076187321133 50-51 21.211231791068954 27.30707133587071 29.834115113754056 21.64758175930628 52-53 22.030652038387856 27.785554043784728 28.307130148300168 21.876663769527248 54-55 21.236135285485137 27.03052228024072 29.268671148880017 22.46467128539413 56-57 22.201702172976127 27.117396779424286 28.625377677877808 22.055523369721783 58-59 22.82862885702363 26.836606952301167 28.34831311885187 21.986451071823335 60-61 21.84312114017927 26.993477525837562 29.848430861000264 21.314970472982907 62-63 21.655896471431944 27.228533971733416 29.349460792500103 21.76610876433454 64-65 21.268351242589763 27.788449155322926 29.073292113146636 21.869907488940676 66-67 21.503814004538672 27.257652695559027 28.557686050749947 22.680847249152354 68-69 21.52809884561198 27.16520563105453 28.91735622936318 22.389339293970316 70-71 22.144742788049125 27.10995440580788 28.53694021513323 22.208362591009767 72-73 21.775925362151654 26.648391278963146 29.39570628503143 22.17997707385377 74-75 21.301784572537652 28.079883683095595 28.462646391604796 22.15568535276196 76-77 21.588665882153787 27.088947560501687 28.46293722713832 22.859449330206203 78-79 21.508687126469944 27.268461992484916 28.71609645192808 22.506754429117063 80-81 22.04968279877916 27.434572270956874 28.135049767895442 22.38069516236853 82-83 21.61159426093549 27.09555080976072 28.916846909284683 22.376008020019103 84-85 21.45904407739448 27.4335034796027 28.40423367967815 22.703218763324674 86-87 21.513395987642664 27.49948693119326 28.134145081752 22.852971999412077 88-89 21.238468903353574 27.289200267936454 27.658523000305795 23.813807828404176 90-91 21.26777658290035 26.7208169138819 28.390833838868122 23.620572664349627 92-93 21.542036894283655 26.977750393397653 28.612500807727976 22.867711904590713 94-95 21.06930073121775 26.955219112773353 28.549709747188594 23.425770408820302 96-97 20.396810300813534 27.130690479592495 29.181952462124144 23.29054675746983 98-99 20.699692932720048 27.535261771670815 27.583269474745677 24.181775820863464 100-101 20.05467977112402 26.73183704190447 28.488816039156834 24.724667147814674 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 307.0 1 228.5 2 190.5 3 235.5 4 214.0 5 203.0 6 336.0 7 633.0 8 956.0 9 1166.5 10 1193.5 11 1153.5 12 1210.5 13 1355.0 14 1520.0 15 1663.5 16 1820.0 17 1998.0 18 2180.0 19 2321.0 20 2519.0 21 2956.0 22 3523.5 23 4121.5 24 4836.0 25 5900.0 26 7408.0 27 9085.0 28 10620.0 29 12605.5 30 14937.0 31 17204.0 32 19915.0 33 21427.5 34 23107.0 35 25326.0 36 27278.5 37 29579.0 38 30715.5 39 30987.0 40 31938.5 41 33766.5 42 35011.0 43 35881.0 44 37074.5 45 38543.5 46 39506.5 47 46223.5 48 62236.0 49 59671.5 50 42853.5 51 36150.0 52 32993.5 53 31507.5 54 29975.0 55 27749.0 56 25261.5 57 22142.5 58 19921.5 59 17673.0 60 14762.0 61 12247.5 62 9876.0 63 7480.5 64 5436.5 65 4098.5 66 3210.0 67 2571.0 68 1916.5 69 1323.0 70 997.5 71 768.5 72 672.5 73 591.0 74 533.0 75 462.0 76 318.5 77 198.0 78 131.0 79 103.0 80 77.5 81 59.5 82 39.0 83 25.5 84 15.5 85 7.5 86 2.5 87 1.5 88 1.5 89 1.0 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012741276548497394 2 0.01674567774945372 3 0.006097610919638038 4 7.280729456284225E-4 5 0.0048234832647883 6 0.0 7 3.6403647281421127E-4 8 0.0018201823640710563 9 0.0 10-11 0.0 12-13 0.0 14-15 7.735775047301988E-4 16-17 0.006188620037841591 18-19 0.0011376139775444102 20-21 6.825683865266461E-4 22-23 6.370638274248697E-4 24-25 5.005501501195405E-4 26-27 9.100911820355282E-5 28-29 1.8201823640710563E-4 30-31 4.095410319159876E-4 32-33 0.003458346491735007 34-35 0.001228623095747963 36-37 0.0018201823640710563 38-39 0.004413942232872311 40-41 9.100911820355282E-5 42-43 0.0018656869231728325 44-45 0.0038678875236509945 46-47 0.0 48-49 0.0023207325141905966 50-51 0.0015471550094603977 52-53 5.460547092213169E-4 54-55 3.1853191371243486E-4 56-57 0.012422744634784958 58-59 0.0029577963416154663 60-61 0.004686969587482969 62-63 2.2752279550888204E-4 64-65 0.005233024296704287 66-67 0.0010466048593408574 68-69 0.002457246191495926 70-71 0.00696219754257179 72-73 0.004140914878261653 74-75 0.006234124596943369 76-77 0.0018201823640710563 78-79 0.006143115478739815 80-81 0.0036858692872438893 82-83 0.0036858692872438893 84-85 0.0027757781052083607 86-87 9.555957411373046E-4 88-89 0.002093209718681715 90-91 0.010875589625324561 92-93 0.0027302735461065845 94-95 6.825683865266461E-4 96-97 3.6403647281421127E-4 98-99 8.190820638319752E-4 100-101 0.0027302735461065845 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1098791.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.057830095891596 #Duplication Level Percentage of deduplicated Percentage of total 1 79.74754756824835 46.299695672813954 2 12.17270396743789 14.13441557498189 3 3.590671289026955 6.253997508855691 4 1.5620602707703446 3.627593191997083 5 0.7814790843486824 2.2685489951304367 6 0.45545798596660425 1.586574141903965 7 0.3120023747512539 1.2679926603976097 8 0.22676922810422553 1.0532583453001287 9 0.15723111599688713 0.8215647676501227 >10 0.8804605757973291 9.393162847453079 >50 0.07024452070842051 2.787348627932023 >100 0.03713144279699125 3.967342582351897 >500 0.0029642736218869646 1.1776120658728961 >1k 0.0026522448195830735 3.180485537189063 >5k 6.24057604607782E-4 2.1804074801701683 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8199 0.7461837601509296 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5630 0.5123813354860024 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5187 0.47206429612182843 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5056 0.460142101637163 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4036 0.3673128010695392 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3611 0.3286339258330292 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3518 0.32017007784009877 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2963 0.269660017237127 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2757 0.2509121388871951 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2130 0.1938494217735675 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1767 0.16081311186567782 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1754 0.15962999332903163 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1634 0.1487088991446053 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1600 0.1456145891256845 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1546 0.14070009674269265 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1462 0.13305533081359422 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1445 0.13150817580413382 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1315 0.11967699043767194 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1221 0.11112213332653799 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1183 0.10766378683480299 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1171 0.10657167741636034 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 9.100911820355282E-5 0.0 0.0 0.0 0.0 7 9.100911820355282E-5 0.0 0.0 0.0 0.0 8 9.100911820355282E-5 0.0 0.0 0.0 0.0 9 9.100911820355282E-5 0.0 0.0 0.0 0.0 10-11 9.100911820355282E-5 0.0 0.0 0.0 0.0 12-13 9.100911820355282E-5 0.0 0.0 0.0 0.0 14-15 9.100911820355282E-5 0.0 0.0 0.0 0.0 16-17 9.100911820355282E-5 0.0 0.0 0.0 0.0 18-19 1.3651367730532923E-4 0.0 0.0 0.0 0.0 20-21 1.8201823640710563E-4 0.0 0.0 1.8201823640710563E-4 0.0 22-23 1.8201823640710563E-4 0.0 0.0 2.2752279550888204E-4 0.0 24-25 1.8201823640710563E-4 0.0 0.0 5.460547092213169E-4 0.0 26-27 1.8201823640710563E-4 0.0 0.0 9.100911820355282E-4 0.0 28-29 4.095410319159877E-4 0.0 0.0 0.0021387142777834914 0.0 30-31 4.550455910177641E-4 0.0 0.0 0.004823483264788299 0.0 32-33 4.550455910177641E-4 0.0 0.0 0.008327334315625082 0.0 34-35 5.005501501195405E-4 0.0 0.0 0.011330635216342325 0.0 36-37 5.460547092213169E-4 0.0 0.0 0.015562559212807532 0.0 38-39 5.460547092213169E-4 0.0 0.0 0.023480352496516626 0.0 40-41 5.460547092213169E-4 0.0 0.0 0.04036254392327567 0.0 42-43 5.460547092213169E-4 0.0 0.0 0.06693720643871309 0.0 44-45 5.460547092213169E-4 0.0 0.0 0.0918282002673848 0.0 46-47 6.370638274248697E-4 0.0 0.0 0.15207623651813676 0.0 48-49 6.370638274248697E-4 0.0 0.0 0.2046340022806885 0.0 50-51 6.370638274248697E-4 0.0 0.0 0.2453150781176766 0.0 52-53 6.370638274248697E-4 0.0 0.0 0.2895000050055015 0.0 54-55 6.370638274248697E-4 0.0 0.0 0.3262676887597369 0.0 56-57 7.280729456284225E-4 0.0 0.0 0.424739554655981 0.0 58-59 7.280729456284225E-4 0.0 0.0 0.550286633217782 0.0 60-61 7.280729456284225E-4 0.0 0.0 0.665913717895396 0.0 62-63 7.280729456284225E-4 0.0 0.0 0.8062042736061725 0.0 64-65 8.190820638319754E-4 0.0 0.0 0.9834445313075917 0.0 66-67 9.100911820355282E-4 0.0 0.0 1.229396673252693 0.0 68-69 0.001001100300239081 0.0 0.0 1.5171675050123272 0.0 70-71 0.0010921094184426338 0.0 0.0 1.7167049966736168 0.0 72-73 0.0011831185366461866 0.0 0.0 1.8713749930605548 0.0 74-75 0.0011831185366461866 0.0 0.0 2.0771011047596857 0.0 76-77 0.0011831185366461866 0.0 0.0 2.3427567207958564 0.0 78-79 0.0011831185366461866 0.0 0.0 2.5879807897953295 0.0 80-81 0.0012741276548497394 0.0 0.0 2.847857326825575 0.0 82-83 0.0012741276548497394 0.0 0.0 3.1825888635782418 0.0 84-85 0.0012741276548497394 0.0 0.0 3.7531250255963142 0.0 86-87 0.0013196322139515158 0.0 0.0 4.4535311992908575 0.0 88-89 0.0013651367730532923 0.0 0.0 5.1588518653683915 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACCGA 140 5.810907E-7 27.140076 6 AGGCGAC 235 1.7644197E-10 24.253937 5 GTACATG 4920 0.0 22.406275 1 GAGTACT 5875 0.0 22.356081 12-13 GCGACAG 265 7.930794E-10 21.507229 7 ACATGGG 4815 0.0 21.308165 3 TACATGG 4945 0.0 21.2331 2 GGCGACA 270 1.0022632E-9 21.108946 6 GTACTTT 6275 0.0 21.006691 14-15 GTAGCGT 115 0.003723423 20.651936 3 GTAAGCG 140 1.5788828E-9 20.355982 94-95 AGTACTT 6100 0.0 20.24659 12-13 GTAAGGT 405 0.0 19.937134 4 TCTACGC 125 0.0060313772 18.999783 3 TAAGGTG 475 0.0 18.998917 5 CATGGGA 2870 0.0 18.866522 4 AGTACAT 3960 0.0 18.716164 2 ACTTTTT 7160 0.0 18.607952 16-17 CCACGTC 230 2.0597072E-6 18.58505 9 TAATACA 705 0.0 18.19045 4 >>END_MODULE