FastQCFastQC Report
Thu 26 May 2016
SRR936498_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936498_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1219504
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT97350.7982753644104489No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT48480.3975386714598722No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46020.37736653590312125No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39690.3254601870924573No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT38930.3192281452131358No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC32400.26568178538159776No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT29880.2450176465185846No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC24880.20401737099673312No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC23820.19532531258610059No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT23000.18860126740051691No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22360.18335323213371993No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG20260.1661331164145423No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA19830.1626070927196631No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC17870.14653498471509727No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA15690.12865886458757003No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA14430.11832679515606345No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA13210.10832272792873168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG56400.025.446471
TACATGG56100.024.5650142
ACATGGG56250.023.3158153
GAGTACT44750.021.97798712-13
GTGTACG1302.9322255E-421.9334831
TTGCGCC1100.002870235421.6002714
TAAGGTG5150.020.302565
AGTACTT46550.020.10745612-13
GTACTTT49550.019.8952514-15
GTATAGG3353.45608E-1119.8601681
GTATATA6950.019.8295521
CATGGGA32250.018.8608884
CATGGGG24450.017.8809594
ACTTTTT56200.017.70871516-17
ATGGGCG2152.3831893E-517.684265
GTGTAAG4353.6379788E-1217.4795571
TACTTTT53900.017.4522514-15
ATGGGAG14550.017.3120385
GTCCTAG5850.017.0593761
AGAGTAC91250.016.95013810-11
Produced by FastQC (version 0.11.5)