##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936498_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1219504 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.43374519476771 31.0 31.0 34.0 30.0 34.0 2 31.471490048413127 31.0 31.0 34.0 28.0 34.0 3 31.518326303152758 31.0 31.0 34.0 28.0 34.0 4 35.148059374959 37.0 35.0 37.0 32.0 37.0 5 35.088754936433176 37.0 35.0 37.0 32.0 37.0 6 35.20883654338157 37.0 35.0 37.0 32.0 37.0 7 35.038160596439205 37.0 35.0 37.0 32.0 37.0 8 35.03438529106916 37.0 35.0 37.0 32.0 37.0 9 36.549437312218735 39.0 37.0 39.0 32.0 39.0 10-11 36.424388931893624 39.0 37.0 39.0 32.0 39.0 12-13 36.34550932182265 39.0 37.0 39.0 32.0 39.0 14-15 37.41967882024167 40.0 37.0 41.0 32.0 41.0 16-17 37.3865809378239 40.0 37.0 41.0 32.0 41.0 18-19 37.386050394258646 40.0 37.0 41.0 32.0 41.0 20-21 37.29491457182593 40.0 37.0 41.0 31.0 41.0 22-23 37.213601185399966 39.0 36.5 41.0 31.0 41.0 24-25 37.0567997316942 39.0 36.0 41.0 31.0 41.0 26-27 36.84511120914732 39.0 36.0 41.0 30.5 41.0 28-29 36.95193168698094 39.0 36.0 41.0 30.5 41.0 30-31 36.91752507576851 39.0 36.0 41.0 30.0 41.0 32-33 36.76656575132185 39.0 36.0 41.0 30.0 41.0 34-35 36.57283165942876 39.0 35.0 41.0 30.0 41.0 36-37 36.322851339561 39.0 35.0 40.0 30.0 41.0 38-39 36.14814137551004 39.0 35.0 40.0 30.0 41.0 40-41 36.03822906689933 39.0 35.0 40.0 29.5 41.0 42-43 35.77044437738621 38.0 35.0 40.0 28.5 41.0 44-45 35.487780277883466 38.0 35.0 40.0 27.0 41.0 46-47 35.13242391988874 38.0 34.0 40.0 26.0 41.0 48-49 34.869943436019895 38.0 34.0 40.0 25.0 41.0 50-51 34.14622174261011 37.5 33.5 39.5 23.5 40.5 52-53 34.34086809063356 38.0 33.5 39.5 24.0 40.5 54-55 34.71449335139532 38.0 34.0 40.0 24.0 41.0 56-57 34.62622836825463 38.0 34.0 40.0 24.0 41.0 58-59 34.411004801952274 38.0 34.0 40.0 24.0 41.0 60-61 34.07167996168934 38.0 33.5 40.0 23.0 41.0 62-63 33.78722579015731 37.0 33.0 40.0 22.0 41.0 64-65 33.48272822393366 37.0 33.0 40.0 22.0 41.0 66-67 33.090015694905475 36.0 32.5 39.0 21.0 41.0 68-69 32.707893947047324 36.0 32.0 39.0 20.0 41.0 70-71 32.283194233065245 35.0 31.5 38.5 18.5 40.0 72-73 31.865833568401577 35.0 31.0 38.0 17.5 40.0 74-75 31.470319080544222 35.0 31.0 37.0 15.5 39.0 76-77 31.05237416195437 35.0 31.0 37.0 12.0 39.0 78-79 30.581648358676972 34.5 30.0 36.0 7.5 39.0 80-81 30.154175796061352 34.0 30.0 36.0 7.0 37.5 82-83 29.740070553274116 34.0 29.5 35.0 2.0 37.0 84-85 29.348564662354534 34.0 29.0 35.0 2.0 37.0 86-87 29.050325378186542 34.0 29.0 35.0 2.0 36.0 88-89 28.64739394048728 34.0 29.0 35.0 2.0 36.0 90-91 28.282136425956786 34.0 28.5 35.0 2.0 35.5 92-93 27.91425448379013 34.0 27.0 35.0 2.0 35.0 94-95 27.484674097009933 34.0 27.0 35.0 2.0 35.0 96-97 26.920290134349703 33.0 25.5 35.0 2.0 35.0 98-99 26.30259474343668 33.0 25.0 35.0 2.0 35.0 100-101 24.310624237394876 31.0 12.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.39436713293450154 1101 2 0.47560959918199686 1101 3 0.5340960468217979 1101 4 0.5387838547511024 1101 5 0.4288902048521166 1101 6 0.453196485626286 1101 7 0.4489736566651388 1101 8 0.6001112685379937 1101 9 0.7110398532721476 1101 10-11 0.8409294377752659 1101 12-13 0.7874570557251914 1101 14-15 0.9450513494966515 1101 16-17 0.9808743033109195 1101 18-19 0.8856770814459551 1101 20-21 0.9493927623160587 1101 22-23 0.8500723381192046 1101 24-25 0.8251435803016776 1101 26-27 0.9648194915034551 1101 28-29 1.007519211233479 1101 30-31 0.8463536340567828 1101 32-33 0.7757626469994534 1101 34-35 0.8807017285935714 1101 36-37 0.846655157482914 1101 38-39 0.923028990229831 1101 40-41 0.931374016515818 1101 42-43 0.7549076650827615 1101 44-45 0.8093086380727357 1101 46-47 0.8910686619269015 1101 48-49 1.003089209933968 1101 50-51 1.1113294413852088 1101 52-53 1.3070063483033962 1101 54-55 1.2359970920608134 1101 56-57 1.156936873833061 1101 58-59 1.2406049448431773 1101 60-61 1.1438124265136906 1101 62-63 1.1953042613293334 1101 64-65 1.206562158635677 1101 66-67 1.2016791577665487 1101 68-69 1.193439872542136 1101 70-71 1.13723669891953 1101 72-73 1.291431664361074 1101 74-75 1.2244512157607836 1101 76-77 1.2024944934112014 1101 78-79 1.1913315109540754 1101 80-81 1.1184254521870685 1101 82-83 1.1784782405520637 1101 84-85 1.040542257280169 1101 86-87 0.9454672067826415 1101 88-89 1.0428454509416731 1101 90-91 1.1885592283239994 1101 92-93 0.9666007157908005 1101 94-95 0.7419432223369675 1101 96-97 0.6080842042357553 1101 98-99 0.42965058873532413 1101 100-101 0.8706047752406256 1102 1 0.3275731293459181 1102 2 0.31588669539686975 1102 3 0.3902984370314506 1102 4 0.40139713050599823 1102 5 0.34905297465665086 1102 6 0.3900458027631686 1102 7 0.20781300327070795 1102 8 0.34188469941241806 1102 9 0.5099557278578644 1102 10-11 0.6034596047980187 1102 12-13 0.631790499786284 1102 14-15 0.5966269551649006 1102 16-17 0.4726499361578078 1102 18-19 0.4598357392242036 1102 20-21 0.31649963473503107 1102 22-23 0.02292151432985179 1102 24-25 0.2900765396771803 1102 26-27 0.3117588126919628 1102 28-29 0.36297104340039255 1102 30-31 0.2881759487199105 1102 32-33 0.4088385382949937 1102 34-35 0.20277826412334576 1102 36-37 0.07929354830660174 1102 38-39 0.2192059256378016 1102 40-41 0.08793079846518026 1102 42-43 0.3124650233197741 1102 44-45 0.2540388146583368 1102 46-47 0.5078603377160249 1102 48-49 0.6328218778941377 1102 50-51 0.5476748213953258 1102 52-53 0.5476844551607201 1102 54-55 0.4374967611358116 1102 56-57 0.5959652000068374 1102 58-59 0.5958790463754937 1102 60-61 0.2619290605911573 1102 62-63 0.5350189108633927 1102 64-65 0.603361371461979 1102 66-67 0.4861629902555791 1102 68-69 0.6017736462699546 1102 70-71 0.7116092625942869 1102 72-73 0.4120362127553996 1102 74-75 0.2659171883657301 1102 76-77 0.4508577987354485 1102 78-79 0.24152327129894147 1102 80-81 0.5944591838886772 1102 82-83 0.5023780092820438 1102 84-85 0.5092317816792864 1102 86-87 0.37013961619349445 1102 88-89 0.09578999143406008 1102 90-91 -0.051577822442158805 1102 92-93 0.11210516880825949 1102 94-95 0.19039913633169547 1102 96-97 0.1445586198322637 1102 98-99 -0.09091929432106483 1102 100-101 0.428505326614534 1103 1 0.2779334983828079 1103 2 0.34444213206809593 1103 3 0.4341232170697893 1103 4 0.2887097392664728 1103 5 0.3739212747148386 1103 6 0.18108156525075003 1103 7 0.3253366485720548 1103 8 0.18156467482050687 1103 9 0.5695317285861208 1103 10-11 0.39965072902011656 1103 12-13 0.5103513126384556 1103 14-15 0.5187491811544831 1103 16-17 0.5489470140220547 1103 18-19 0.5661924300298153 1103 20-21 0.425871439535463 1103 22-23 0.3169435309452169 1103 24-25 0.41829697101180585 1103 26-27 0.5966256549145754 1103 28-29 0.6405736097187145 1103 30-31 0.40471819362836925 1103 32-33 0.3114214799037285 1103 34-35 0.2966129059905356 1103 36-37 0.4510073164751276 1103 38-39 0.4381987195763699 1103 40-41 0.35174873789046046 1103 42-43 0.5084490821187018 1103 44-45 0.5446743320184595 1103 46-47 0.45282819936809204 1103 48-49 0.7317317335222313 1103 50-51 0.15382009306067346 1103 52-53 0.47178168553699606 1103 54-55 0.5607344951975719 1103 56-57 0.7794072553307032 1103 58-59 0.9408146509418813 1103 60-61 1.019207519058682 1103 62-63 0.9388453004577499 1103 64-65 0.8475653206791662 1103 66-67 0.9080508741247968 1103 68-69 0.841601450162706 1103 70-71 0.9489475318993925 1103 72-73 0.8289438730546443 1103 74-75 0.7735891305061493 1103 76-77 0.7421252647269796 1103 78-79 0.873375593215421 1103 80-81 0.8623321820033887 1103 82-83 0.867452853636653 1103 84-85 0.6899227495633653 1103 86-87 0.6629003741624437 1103 88-89 0.6540343154403239 1103 90-91 0.8253772274079019 1103 92-93 0.7803915061843938 1103 94-95 0.6773239674272666 1103 96-97 0.6737425419508618 1103 98-99 0.9041647983840839 1103 100-101 0.8862729515827077 1104 1 0.24038965501336307 1104 2 0.27398217985708584 1104 3 0.3852259334691368 1104 4 0.22821954663627508 1104 5 0.2793905924008797 1104 6 0.27337171762025747 1104 7 0.37912994658873345 1104 8 0.47207100914439337 1104 9 0.5150065540545228 1104 10-11 0.6338581359397182 1104 12-13 0.47101706583143965 1104 14-15 0.4808835515872687 1104 16-17 0.2470228822274123 1104 18-19 0.4090131554556322 1104 20-21 0.4321393482469702 1104 22-23 0.4326584089171561 1104 24-25 0.4265229113197151 1104 26-27 0.41864159305978177 1104 28-29 0.21135665772248302 1104 30-31 0.40778189756710503 1104 32-33 0.3701646728160526 1104 34-35 0.3537535288039706 1104 36-37 0.4521601342371184 1104 38-39 0.4187955731060313 1104 40-41 0.27054056541904004 1104 42-43 0.37372561993868203 1104 44-45 0.1432439923693849 1104 46-47 0.24331301891712798 1104 48-49 0.13213759571841877 1104 50-51 0.1353467590338795 1104 52-53 0.44996794885838653 1104 54-55 0.6623246428525391 1104 56-57 0.5640868273700264 1104 58-59 0.5916002956854314 1104 60-61 0.3240365196281658 1104 62-63 0.29570151665615896 1104 64-65 0.42064502412699056 1104 66-67 0.3886318254295702 1104 68-69 0.46022044856949407 1104 70-71 0.4163910004253353 1104 72-73 0.18451780867195922 1104 74-75 -0.06806923582274393 1104 76-77 0.31607697135375545 1104 78-79 0.37044434545576266 1104 80-81 0.37498380504058204 1104 82-83 0.2297089041525986 1104 84-85 0.4744305775738802 1104 86-87 0.3453486582562526 1104 88-89 0.16604741737695505 1104 90-91 0.11725489477226958 1104 92-93 0.0528963348482101 1104 94-95 -0.1867606518659919 1104 96-97 -0.04711373112139583 1104 98-99 -0.07455035422863432 1104 100-101 0.05749071028945352 1105 1 0.06437467982647149 1105 2 0.1804120794924522 1105 3 0.15810716788339008 1105 4 0.1977879851338642 1105 5 0.26498299560952887 1105 6 0.14502879576753003 1105 7 0.16192125818604808 1105 8 -0.0707942224461533 1105 9 0.303327617943566 1105 10-11 0.28063073131094285 1105 12-13 0.2828791014531191 1105 14-15 0.15856636845191474 1105 16-17 0.08310607852718732 1105 18-19 -0.05069093477785458 1105 20-21 -0.28640099385130924 1105 22-23 -0.3817757060513216 1105 24-25 -0.3384881233671635 1105 26-27 -0.45545175643876235 1105 28-29 -0.16062282079320056 1105 30-31 -0.3599354159071595 1105 32-33 -0.4487037685309758 1105 34-35 -0.5172109515454579 1105 36-37 -0.29934018793345274 1105 38-39 -0.4156599953894542 1105 40-41 -0.6566026961604834 1105 42-43 -0.4884471884971333 1105 44-45 -0.5516744906622009 1105 46-47 -0.3466656378567805 1105 48-49 -0.3326674520188959 1105 50-51 -0.2987302935052014 1105 52-53 -0.37593355426727015 1105 54-55 -0.43834791694663977 1105 56-57 -0.17720997123797133 1105 58-59 -0.2558438943520045 1105 60-61 -0.23209722206365058 1105 62-63 -0.4933266220251724 1105 64-65 -0.5367514791503183 1105 66-67 -0.7342750999049912 1105 68-69 -0.34892956342573456 1105 70-71 -0.4126536190503103 1105 72-73 -0.3636549648352343 1105 74-75 -0.44070707289543165 1105 76-77 -0.2914847994538192 1105 78-79 -0.29596342046364654 1105 80-81 -0.38776652057689276 1105 82-83 -0.27732002134189315 1105 84-85 -0.5762542991602579 1105 86-87 -0.32057654118157686 1105 88-89 -0.378729696654883 1105 90-91 -0.43642166435407503 1105 92-93 -0.49179874288175895 1105 94-95 -0.44242942926312523 1105 96-97 -0.36960624674506093 1105 98-99 -0.6531133263969551 1105 100-101 -0.00887344081176522 1106 1 -0.15309940720744208 1106 2 -0.1947277456382679 1106 3 -0.20706376885781097 1106 4 0.05082948960522771 1106 5 0.2640913250084793 1106 6 0.21331438053684337 1106 7 0.3073416080906526 1106 8 0.1210334034020022 1106 9 0.26008408449263953 1106 10-11 0.2998071841224217 1106 12-13 0.33347075340159194 1106 14-15 0.35201184232419536 1106 16-17 0.09775001082221024 1106 18-19 0.2534608960803624 1106 20-21 0.19370301778081256 1106 22-23 0.12642699929167378 1106 24-25 -0.12396911351450512 1106 26-27 -0.08922448615187051 1106 28-29 -0.1994359524277982 1106 30-31 -0.03913934390710949 1106 32-33 -0.13662929770787002 1106 34-35 -0.3225417137384028 1106 36-37 -0.1716945802275518 1106 38-39 -0.054408866963989055 1106 40-41 -0.06270136883877342 1106 42-43 -0.02656901250869481 1106 44-45 -0.0591903162932752 1106 46-47 -0.08341580570228047 1106 48-49 0.09250832221520255 1106 50-51 0.40630717492517476 1106 52-53 0.0881046121126019 1106 54-55 0.3253828970528687 1106 56-57 0.10537461155040972 1106 58-59 -0.0016247869192582698 1106 60-61 0.036429296419285606 1106 62-63 0.10999397921873566 1106 64-65 0.1548946023420612 1106 66-67 -0.011930587387560365 1106 68-69 0.21755220105256967 1106 70-71 0.21338188947590453 1106 72-73 0.39479943958027874 1106 74-75 0.07131525927428228 1106 76-77 -0.09223848527470935 1106 78-79 0.25934091263482273 1106 80-81 0.24740459881563837 1106 82-83 0.15551769959011708 1106 84-85 0.3148243455225703 1106 86-87 0.3735773984693118 1106 88-89 0.406653879447898 1106 90-91 0.5480125676053724 1106 92-93 0.29781763950378703 1106 94-95 0.07846975771704479 1106 96-97 0.2427107629094465 1106 98-99 0.3908699449548507 1106 100-101 0.6618394141337411 1107 1 0.17571431359518286 1107 2 0.20482393938031862 1107 3 0.1864459852129272 1107 4 0.2466404527678847 1107 5 0.194864505151358 1107 6 0.24689477818041183 1107 7 0.29920542337288225 1107 8 0.48396387664689655 1107 9 0.44541760366820426 1107 10-11 0.4918124982693044 1107 12-13 0.4950789532784441 1107 14-15 0.6473924866203973 1107 16-17 0.643829768897966 1107 18-19 0.695819075226396 1107 20-21 0.6832864430060752 1107 22-23 0.6255373384125775 1107 24-25 0.7014857735775095 1107 26-27 0.7580043767936004 1107 28-29 0.6010180083936021 1107 30-31 0.3447107308120536 1107 32-33 0.2897859996955745 1107 34-35 0.25406615973832203 1107 36-37 0.6190397141924109 1107 38-39 0.6333206055557739 1107 40-41 0.35420157035023436 1107 42-43 0.4619134739192319 1107 44-45 0.5916532957034306 1107 46-47 0.5717146749527799 1107 48-49 0.4918235876843582 1107 50-51 0.6257195135556017 1107 52-53 0.4943413245399739 1107 54-55 0.579965290148003 1107 56-57 0.6078698081756073 1107 58-59 0.617401870267102 1107 60-61 0.8170213686013454 1107 62-63 0.8189473992745278 1107 64-65 0.5362869723421895 1107 66-67 0.5040466986924486 1107 68-69 0.5130285599797872 1107 70-71 0.8197896532116218 1107 72-73 0.9026943528548834 1107 74-75 0.6607153711324862 1107 76-77 0.8183045033859742 1107 78-79 0.8530547991075679 1107 80-81 1.0299727149929119 1107 82-83 1.0860796018147525 1107 84-85 0.7814622830972233 1107 86-87 0.7362904186584842 1107 88-89 0.8001945092060616 1107 90-91 0.894039484147509 1107 92-93 0.9990627236532639 1107 94-95 1.3458421837874148 1107 96-97 1.2577644248541908 1107 98-99 1.2259452551672894 1107 100-101 1.1048358378469771 1108 1 0.10980948655495126 1108 2 0.15065002165243868 1108 3 0.022267125442791524 1108 4 0.08251429250049824 1108 5 0.3826785063552478 1108 6 0.32461366263733993 1108 7 0.537879129131646 1108 8 0.261653952042316 1108 9 0.4097318426054102 1108 10-11 0.12704293562833158 1108 12-13 0.3857538348216707 1108 14-15 0.5342689051153471 1108 16-17 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-2.2688416585317057 2316 62-63 -2.258010756985687 2316 64-65 -2.1861484640749467 2316 66-67 -3.030771869467088 2316 68-69 -2.494625902262772 2316 70-71 -1.9640768064157115 2316 72-73 -1.684112610204938 2316 74-75 -1.9330742660038318 2316 76-77 -2.8478200471207273 2316 78-79 -2.7539515755246633 2316 80-81 -2.780323950584073 2316 82-83 -2.53650702780277 2316 84-85 -2.626794500165282 2316 86-87 -2.6545104966733213 2316 88-89 -3.0689342910554593 2316 90-91 -3.125109747627583 2316 92-93 -3.308803409069842 2316 94-95 -3.5179409152645604 2316 96-97 -3.5026213221219464 2316 98-99 -3.942434934437159 2316 100-101 -4.417924051005588 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2511.0 3 953.0 4 891.0 5 1005.0 6 1075.0 7 1339.0 8 1627.0 9 2178.0 10 2701.0 11 3526.0 12 4551.0 13 5595.0 14 6642.0 15 7991.0 16 9717.0 17 12479.0 18 10907.0 19 7635.0 20 7466.0 21 8273.0 22 9096.0 23 10176.0 24 11924.0 25 13651.0 26 15699.0 27 18777.0 28 22606.0 29 27425.0 30 32941.0 31 40799.0 32 51339.0 33 66138.0 34 86476.0 35 117928.0 36 163639.0 37 216874.0 38 182693.0 39 32251.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.21213950390883 18.826581327524497 14.094736945702916 21.86654222286376 2 15.25929667856856 21.025919006998446 39.680043037065346 24.034741277367655 3 20.583780343891284 24.69286048760166 29.993431179509216 24.72992798899784 4 12.474755511952477 16.512068957031463 38.39524624347253 32.61792928754353 5 13.204982324937303 38.25886070705173 34.32973815967691 14.206418808334064 6 31.062502615455433 36.74027686493255 17.36490095731823 14.832319562293788 7 27.94488456370774 33.604568563727426 20.74186663429343 17.708680238271402 8 26.112758426943252 33.74132083287195 19.883771956806147 20.262148783378652 9 26.015341979497553 17.76867943005194 21.111420039384253 35.104558551066255 10-11 24.935329285311685 26.52590479519094 27.296005224524087 21.242760694973285 12-13 26.88168911360669 24.18822039171835 27.46438113021331 21.465709364461652 14-15 22.970829351913746 25.80800603672065 27.03068803031484 24.190476581050767 16-17 21.548379529377797 29.25812367940995 27.439482525558752 21.754014265653502 18-19 21.319799244439213 28.36178819607504 29.407482356782022 20.910930202703728 20-21 22.697476613667213 26.57130319596127 29.10658262214002 21.6246375682315 22-23 21.910780227057906 26.70327259455791 29.381465070932528 22.004482107451658 24-25 22.507014452868574 26.87182258508266 29.326993845158437 21.294169116890327 26-27 22.11689812774224 26.550774172412705 30.11582552491321 21.216502174931843 28-29 21.201326643533637 28.08670334658091 29.574400650775317 21.137569359110138 30-31 21.61712781691599 27.05994317680918 29.32356292641483 21.999366079859996 32-33 21.525008230909926 27.27758400345719 29.427747781139384 21.769659984493497 34-35 22.77247289713725 26.87628974611605 29.014464837108118 21.336772519638583 36-37 22.361854683924435 26.495297193815315 29.748247441533206 21.394600680727045 38-39 21.80592634932142 27.282266096504195 29.659997777629254 21.251809776545134 40-41 21.903277244046414 27.479418051268006 29.28944868381723 21.327856020868346 42-43 21.570544832528903 27.74794299930955 28.41026688118187 22.27124528697968 44-45 22.079821432744737 27.187881436787432 28.485847913606115 22.246449216861716 46-47 22.668646217593977 27.781488410729754 28.159972971831582 21.389892399844687 48-49 22.52055560612285 27.684569949741572 28.08354592098218 21.711328523153398 50-51 21.60025453451099 28.19113212915986 28.647719808672704 21.56089352765644 52-53 22.35445819786059 28.55862467660794 27.47305237003842 21.613864755493054 54-55 21.55167854139495 27.738441359397736 28.15399193007762 22.555888169129698 56-57 22.262121289154432 27.9951224552386 27.725578244903915 22.017178010703045 58-59 23.101782623794914 27.511005837388723 27.48874200316286 21.8984695356535 60-61 22.169923492607566 27.775135507466235 28.77448769567606 21.280453304250138 62-63 21.871420125281777 27.891759758784605 28.507016451945933 21.729803663987678 64-65 21.600646513815295 28.634887391053525 27.954834236531063 21.809631858600113 66-67 21.870330978766255 27.999202935035928 27.589846113980286 22.54061997221753 68-69 21.656800754776086 27.897562353697552 28.070757387953186 22.374879503573172 70-71 22.33902921172504 27.94162329458857 27.596409845738506 22.12293764794789 72-73 22.075002419115442 27.429201921515695 28.487134406053855 22.008661253315008 74-75 21.411181041021752 28.991164230509032 27.67686914450891 21.92078558396031 76-77 21.949352442236012 27.96325278308178 27.537164817565714 22.550229957116493 78-79 21.954634774373567 28.188733710317322 27.666036537123023 22.190594978186088 80-81 22.368430765445265 28.163300457257684 27.44403206955238 22.024236707744674 82-83 22.082824738438376 27.971072311431055 27.954794541329754 21.99130840880082 84-85 21.80122479021316 28.241849937571374 27.715440270462544 22.241485001752924 86-87 21.9777047642361 28.237748055717176 27.523016282074465 22.261530897972253 88-89 21.780933523640282 28.12834812230433 27.077819331681475 23.012899022373915 90-91 21.912549909356365 27.46114584542154 27.651946490527013 22.974357754695077 92-93 22.33447044114288 28.020706597890708 27.75432894441704 21.89049401654937 94-95 21.86884058832671 27.904515613717784 27.752312668804013 22.474331129151498 96-97 21.097179107543578 28.07099403872797 28.248532438069645 22.583294415658806 98-99 21.586650492006584 28.558445723434637 26.91998218490786 22.934921599650913 100-101 21.47311487016127 27.816091576839003 27.576747122226465 23.134046430773257 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 174.0 1 141.0 2 156.0 3 200.0 4 195.5 5 218.0 6 356.0 7 664.0 8 1007.0 9 1216.0 10 1187.0 11 1127.5 12 1207.0 13 1309.5 14 1406.5 15 1508.0 16 1633.0 17 1768.5 18 1879.5 19 2043.0 20 2350.0 21 2714.0 22 3145.0 23 3826.5 24 4696.5 25 5890.5 26 7647.0 27 9596.5 28 11368.5 29 13392.5 30 15889.0 31 18529.0 32 21621.5 33 23821.5 34 25778.0 35 27876.0 36 30153.0 37 32834.5 38 34248.0 39 34828.0 40 35889.0 41 37601.0 42 38950.0 43 40277.0 44 41805.0 45 43633.0 46 44754.0 47 51192.0 48 67939.0 49 66240.0 50 48643.0 51 40806.5 52 37344.5 53 35725.0 54 34260.0 55 31440.0 56 28280.5 57 25065.0 58 22590.0 59 20146.5 60 16777.5 61 13622.5 62 10997.5 63 8624.0 64 6461.5 65 5019.5 66 3847.0 67 2971.0 68 2193.0 69 1484.0 70 1136.0 71 921.5 72 730.5 73 635.5 74 552.0 75 447.5 76 316.0 77 191.5 78 129.0 79 101.5 80 71.0 81 45.0 82 34.0 83 29.0 84 19.5 85 11.0 86 8.0 87 6.5 88 4.5 89 3.5 90 2.5 91 3.5 92 2.0 93 2.0 94 2.5 95 1.5 96 1.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01738411682126504 2 0.007380049593933271 3 0.008774058961676223 4 0.034932234744617485 5 0.04551030582925517 6 0.06453443367139428 7 0.031980214907044176 8 0.02861819231425235 9 0.019270129495270207 10-11 0.05223435101483882 12-13 0.04858532649339404 14-15 0.0245181647620672 16-17 0.028864193967383458 18-19 0.033579225652396384 20-21 0.025338170272504235 22-23 0.017343116545743185 24-25 0.004715031685012923 26-27 0.0033210223172699723 28-29 0.002911019562051457 30-31 0.008815059237198074 32-33 0.0012710085411773966 34-35 0.002419016255789239 36-37 0.0059450399506684685 38-39 0.0070110471142366074 40-41 0.0040590272766633 42-43 8.200055104370302E-4 44-45 0.0015990107453522089 46-47 0.002911019562051457 48-49 5.740038573059211E-4 50-51 0.0018860126740051694 52-53 7.380049593933272E-4 54-55 0.0030750206641388628 56-57 0.0031160209396607144 58-59 0.0030750206641388628 60-61 0.0011480077146118423 62-63 0.0010660071635681392 64-65 0.0033210223172699723 66-67 0.0024600165313110904 68-69 0.005699038297537359 70-71 0.009307062543460292 72-73 0.004100027552185151 74-75 0.0023780159802673875 76-77 0.0030750206641388628 78-79 0.036285243836838586 80-81 0.022878153741193143 82-83 0.0040590272766633 84-85 0.009143061441372887 86-87 0.007831052624673637 88-89 0.005781038848581062 90-91 0.013817092850863959 92-93 0.014965100565475798 94-95 0.00651904380797439 96-97 0.0040590272766633 98-99 0.02566617247667904 100-101 0.045059302798514805 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1219504.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.64877378063682 #Duplication Level Percentage of deduplicated Percentage of total 1 80.21533906092749 47.847466133823445 2 11.986369537536424 14.299444899912533 3 3.5294867080384438 6.315886626286489 4 1.4899574758259029 3.5549654567323175 5 0.7563495417333853 2.2557661356971517 6 0.43511201196633936 1.557233878261075 7 0.2908622031057076 1.2144701628072996 8 0.19744977743006464 0.9422109685570415 9 0.1561686588632788 0.8383742103745045 >10 0.842707252808896 9.355550096115492 >50 0.06204998413469931 2.530544508277646 >100 0.03332768067296677 3.765363908732258 >500 0.001790325440946371 0.7455634996117968 >1k 0.002892064173836445 3.9774613362388265 >5k 1.3771734161125928E-4 0.7996981785721509 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9735 0.7982753644104489 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4848 0.3975386714598722 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4602 0.37736653590312125 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3969 0.3254601870924573 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3893 0.3192281452131358 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3240 0.26568178538159776 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2988 0.2450176465185846 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2488 0.20401737099673312 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2382 0.19532531258610059 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2300 0.18860126740051691 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2236 0.18335323213371993 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2026 0.1661331164145423 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1983 0.1626070927196631 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1787 0.14653498471509727 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1569 0.12865886458757003 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1443 0.11832679515606345 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1321 0.10832272792873168 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 8.200055104370302E-5 0.0 0.0 0.0 0.0 8 8.200055104370302E-5 0.0 0.0 0.0 0.0 9 8.200055104370302E-5 0.0 0.0 0.0 0.0 10-11 8.200055104370302E-5 0.0 0.0 0.0 0.0 12-13 8.200055104370302E-5 0.0 0.0 0.0 0.0 14-15 8.200055104370302E-5 0.0 0.0 0.0 0.0 16-17 8.200055104370302E-5 0.0 0.0 0.0 0.0 18-19 8.200055104370302E-5 0.0 0.0 0.0 0.0 20-21 8.200055104370302E-5 0.0 0.0 0.0 0.0 22-23 8.200055104370302E-5 0.0 0.0 8.200055104370302E-5 0.0 24-25 2.46001653131109E-4 0.0 0.0 3.280022041748121E-4 0.0 26-27 2.46001653131109E-4 0.0 0.0 3.690024796966636E-4 0.0 28-29 3.28002204174812E-4 0.0 0.0 0.0012300082656555452 0.0 30-31 4.100027552185151E-4 0.0 0.0 0.0035260236948792293 0.0 32-33 4.100027552185151E-4 0.0 0.0 0.006478043532452538 0.0 34-35 4.100027552185151E-4 0.0 0.0 0.009922066676288064 0.0 36-37 4.100027552185151E-4 0.0 0.0 0.013694092024298403 0.0 38-39 4.100027552185151E-4 0.0 0.0 0.021566144924493894 0.0 40-41 4.100027552185151E-4 0.0 0.0 0.03575224025505451 0.0 42-43 5.740038573059211E-4 0.0 0.0 0.05735938545507026 0.0 44-45 8.200055104370302E-4 0.0 0.0 0.07806452459360527 0.0 46-47 9.84006612524436E-4 0.0 0.0 0.12533784227030004 0.0 48-49 9.84006612524436E-4 0.0 0.0 0.16855213267033153 0.0 50-51 9.84006612524436E-4 0.0 0.0 0.2018033561185531 0.0 52-53 9.84006612524436E-4 0.0 0.0 0.23903160629239428 0.0 54-55 9.84006612524436E-4 0.0 0.0 0.2691668088009551 0.0 56-57 0.0010660071635681392 0.0 0.0 0.3537503772025348 0.0 58-59 0.0010660071635681392 0.0 0.0 0.45502105774150803 0.0 60-61 0.0010660071635681392 0.0 0.0 0.5552257311169131 0.0 62-63 0.0010660071635681392 0.0 0.0 0.6689194951390074 0.0 64-65 0.0010660071635681392 0.0 0.0 0.8194725068552461 0.0 66-67 0.0010660071635681392 0.0 0.0 1.0291479158739947 0.0 68-69 0.0010660071635681392 0.0 0.0 1.2625214841443735 0.0 70-71 0.0011070074390899906 0.0 0.0 1.422012555924376 0.0 72-73 0.0012300082656555452 0.0 0.0 1.5536644406250408 0.0 74-75 0.0013120088166992483 0.0 0.0 1.7324256419003137 0.0 76-77 0.0013120088166992483 0.0 0.0 1.9546471352287487 0.0 78-79 0.0013120088166992483 0.0 0.0 2.1595255120114407 0.0 80-81 0.0015580104698303572 0.0 0.0 2.3873230428108476 0.0 82-83 0.0015580104698303572 0.0 0.0 2.6775639932300344 0.0 84-85 0.0015580104698303572 0.0 0.0 3.1892884320182633 0.0 86-87 0.0015580104698303572 0.0 0.0 3.8317217491701543 0.0 88-89 0.0015580104698303572 0.0 0.0 4.464438001023367 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 5640 0.0 25.44647 1 TACATGG 5610 0.0 24.565014 2 ACATGGG 5625 0.0 23.315815 3 GAGTACT 4475 0.0 21.977987 12-13 GTGTACG 130 2.9322255E-4 21.933483 1 TTGCGCC 110 0.0028702354 21.600271 4 TAAGGTG 515 0.0 20.30256 5 AGTACTT 4655 0.0 20.107456 12-13 GTACTTT 4955 0.0 19.89525 14-15 GTATAGG 335 3.45608E-11 19.860168 1 GTATATA 695 0.0 19.829552 1 CATGGGA 3225 0.0 18.860888 4 CATGGGG 2445 0.0 17.880959 4 ACTTTTT 5620 0.0 17.708715 16-17 ATGGGCG 215 2.3831893E-5 17.68426 5 GTGTAAG 435 3.6379788E-12 17.479557 1 TACTTTT 5390 0.0 17.45225 14-15 ATGGGAG 1455 0.0 17.312038 5 GTCCTAG 585 0.0 17.059376 1 AGAGTAC 9125 0.0 16.950138 10-11 >>END_MODULE