FastQCFastQC Report
Thu 26 May 2016
SRR936498_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936498_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1219504
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT92150.7556350778677233No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60030.4922493079153492No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53590.43944095304320446No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT52000.4264028654272557No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT43120.3535863761004474No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC36500.299302011309516No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT33860.2776538658339784No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31780.26059775121688816No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC30510.25018368123433793No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT23250.1906512811766095No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA19710.16162308610713866No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC17890.14669898581718468No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC17020.13956493787638252No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG16950.1389909340190766No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA16600.136120914732547No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA15170.12439483593329748No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14700.12054081003424343No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA14570.1194748028706753No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC12610.10340269486610949No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT12540.10282869100880357No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTCG1109.498226E-525.9006029
GTACATG52050.024.6276131
TACATGG52950.023.3229452
TAAGGTG5450.022.6531285
ACATGGG52000.022.5578843
GAGTACT63300.022.31693612-13
GTACTTT68150.020.62420314-15
CATGGGG20750.020.5981944
AGTACTT64850.019.76992812-13
GTAAGGT5450.019.1703874
GTAGGAC2502.1769847E-718.9961133
ATGGGAG14050.018.5882155
ACTTTTT77300.018.2136316-17
GTACTAG2152.1845266E-517.864191
ATTACGA1600.001193691417.8066655
GGTAAGG5100.017.6924573
GTGTAGG5700.017.6878991
CATGGGA30950.017.4923824
ACGATTC1650.001467097417.267078
GTATAAG4254.9112714E-1116.944711
Produced by FastQC (version 0.11.5)