##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936498_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1219504 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.481070992797072 31.0 31.0 34.0 30.0 34.0 2 31.708842283420143 31.0 31.0 34.0 30.0 34.0 3 31.977888551411066 31.0 31.0 34.0 30.0 34.0 4 35.78091830777103 37.0 35.0 37.0 35.0 37.0 5 35.59892464477361 37.0 35.0 37.0 33.0 37.0 6 35.69942534013828 37.0 35.0 37.0 33.0 37.0 7 35.63577241239061 37.0 35.0 37.0 33.0 37.0 8 35.619763444810346 37.0 35.0 37.0 33.0 37.0 9 37.251907332817275 39.0 37.0 39.0 34.0 39.0 10-11 37.13662685813249 39.0 37.0 39.0 33.0 39.0 12-13 37.011748628950784 39.0 37.0 39.0 33.0 39.0 14-15 38.11635222188693 40.0 37.0 41.0 33.0 41.0 16-17 38.04863165680473 40.0 37.0 41.0 33.0 41.0 18-19 38.13341981658117 40.0 37.5 41.0 33.0 41.0 20-21 38.20678283958068 40.0 37.5 41.0 33.0 41.0 22-23 38.13491960665976 40.0 37.0 41.0 33.0 41.0 24-25 38.01932916989202 40.0 37.0 41.0 33.0 41.0 26-27 37.80698792295884 40.0 37.0 41.0 33.0 41.0 28-29 37.71882462050145 40.0 37.0 41.0 32.5 41.0 30-31 37.69913177816555 40.0 37.0 41.0 33.0 41.0 32-33 37.495048396725224 39.5 36.0 41.0 32.0 41.0 34-35 37.30662712053425 39.0 36.0 41.0 32.0 41.0 36-37 37.05847787297131 39.0 36.0 41.0 31.0 41.0 38-39 36.882328799249535 39.0 35.0 41.0 31.0 41.0 40-41 36.65402778506672 39.0 35.0 41.0 30.5 41.0 42-43 36.404578418766974 39.0 35.0 40.5 30.0 41.0 44-45 36.09785289757147 39.0 35.0 40.0 30.0 41.0 46-47 35.656609982419084 38.5 35.0 40.0 28.5 41.0 48-49 35.11530671486112 38.0 34.5 40.0 26.0 41.0 50-51 34.93672714480641 38.0 34.0 40.0 26.0 41.0 52-53 34.68845325640588 38.0 34.0 40.0 24.5 41.0 54-55 34.43767589118198 38.0 34.0 40.0 24.0 41.0 56-57 34.16451073551214 38.0 33.5 40.0 23.5 41.0 58-59 33.790103189493436 37.5 33.0 40.0 22.5 41.0 60-61 33.39441076044031 37.0 32.5 40.0 22.0 41.0 62-63 33.134302552513155 36.5 32.0 39.0 22.0 40.5 64-65 32.71965815610281 36.0 32.0 39.0 20.0 40.0 66-67 32.30420031422611 35.5 31.0 39.0 19.0 40.0 68-69 31.848896764586257 35.0 31.0 38.0 18.0 40.0 70-71 31.3547101936525 35.0 30.5 37.5 14.5 39.5 72-73 30.93944546307351 34.5 30.0 37.0 12.5 39.0 74-75 30.579910767000356 34.0 30.0 36.5 8.0 39.0 76-77 29.136552647633792 32.5 28.0 35.0 7.5 37.5 78-79 29.941420036342645 34.0 29.0 36.0 7.0 38.0 80-81 29.760310339285482 34.0 29.0 35.0 4.5 37.0 82-83 29.407540278670673 34.0 29.0 35.0 2.0 37.0 84-85 28.94850898398037 34.0 29.0 35.0 2.0 36.0 86-87 28.585904597278894 34.0 29.0 35.0 2.0 36.0 88-89 28.166997402222542 33.0 27.5 35.0 2.0 35.5 90-91 27.698043220850444 33.0 27.0 35.0 2.0 35.0 92-93 27.257348889384538 33.0 26.0 35.0 2.0 35.0 94-95 26.7207807436466 32.5 25.0 34.0 2.0 35.0 96-97 26.046293411091725 32.0 24.5 34.0 2.0 35.0 98-99 25.212519188128944 32.0 21.0 34.0 2.0 35.0 100-101 22.49898975321114 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5289307594045205 1101 2 0.4921508684337539 1101 3 0.31308017972054003 1101 4 0.239341968659609 1101 5 0.39165804282720984 1101 6 0.2666253931077449 1101 7 0.22539821998118725 1101 8 0.2790475088651476 1101 9 0.44669664109444795 1101 10-11 0.38399432369885034 1101 12-13 0.31590484409355213 1101 14-15 0.5901418595359686 1101 16-17 0.44028591762533154 1101 18-19 0.45948206419308946 1101 20-21 0.27801376687810375 1101 22-23 0.23367815785387336 1101 24-25 0.13067119013199857 1101 26-27 0.2836356439488057 1101 28-29 0.30487274402355524 1101 30-31 0.19825028539649736 1101 32-33 0.2894729139007026 1101 34-35 0.40364342352183513 1101 36-37 0.5998812201185189 1101 38-39 0.4222988739151248 1101 40-41 0.5989242626671114 1101 42-43 0.580362238602369 1101 44-45 0.5828749790203247 1101 46-47 0.6344239248528396 1101 48-49 0.8463874510712586 1101 50-51 0.7838863833872765 1101 52-53 0.7181666282693797 1101 54-55 0.7320239342351442 1101 56-57 0.6524486348147533 1101 58-59 0.6848729799480822 1101 60-61 0.745052076105921 1101 62-63 0.5663408697222323 1101 64-65 0.6613970412430845 1101 66-67 0.5400534852075864 1101 68-69 0.3218499397783141 1101 70-71 0.4241450094359571 1101 72-73 0.5965002925118625 1101 74-75 0.34500073811519627 1101 76-77 0.7587540755500015 1101 78-79 0.7018419128031788 1101 80-81 0.4224906103485466 1101 82-83 0.6090946383694416 1101 84-85 0.6896808594286767 1101 86-87 0.6443400723006008 1101 88-89 0.5953574778647841 1101 90-91 0.5833262049033223 1101 92-93 0.8102341535710771 1101 94-95 0.6532760733030507 1101 96-97 0.4129505352091982 1101 98-99 0.6704831031625105 1101 100-101 0.4850427395052854 1102 1 0.44402144292194023 1102 2 0.517257758468503 1102 3 0.23866609313646237 1102 4 0.12386487043151106 1102 5 0.23408111849201418 1102 6 0.20973208685238376 1102 7 0.26082795692726535 1102 8 0.07298692568218001 1102 9 0.10829940022885864 1102 10-11 0.3149502188195257 1102 12-13 0.36731114953607147 1102 14-15 0.5026814006700704 1102 16-17 0.3796310132664331 1102 18-19 0.29404378359384964 1102 20-21 0.3353565900836273 1102 22-23 0.31338924648389366 1102 24-25 0.3424207264595083 1102 26-27 0.12785521457675486 1102 28-29 0.18609300166691867 1102 30-31 0.3188340723361591 1102 32-33 0.4605947999970752 1102 34-35 0.36189591300898627 1102 36-37 0.4449608182774156 1102 38-39 0.305475112241524 1102 40-41 0.5143804736135564 1102 42-43 0.4228809427856177 1102 44-45 0.39850004979800957 1102 46-47 0.3885878547823012 1102 48-49 0.2823934127544163 1102 50-51 0.466615810043983 1102 52-53 0.19045354653970747 1102 54-55 0.5198324305773454 1102 56-57 0.26496974229366543 1102 58-59 0.2879913504381335 1102 60-61 0.42226335976778984 1102 62-63 0.42246650546313447 1102 64-65 0.3600376063573023 1102 66-67 0.6002491210239853 1102 68-69 0.42064646745648204 1102 70-71 0.2317514402021672 1102 72-73 0.37853490248591726 1102 74-75 -0.13546933896642344 1102 76-77 0.25815356620231356 1102 78-79 0.4744586865486191 1102 80-81 0.35488974113564353 1102 82-83 0.5231299479416052 1102 84-85 0.6431928167682912 1102 86-87 0.49637003925843004 1102 88-89 0.6087555366058588 1102 90-91 0.6866037465457637 1102 92-93 0.7067848580764355 1102 94-95 0.5306667862639856 1102 96-97 0.5437112097425683 1102 98-99 0.11175807038457819 1102 100-101 0.12582616355988563 1103 1 0.4888924343688643 1103 2 0.4812752762428829 1103 3 0.2881737932788191 1103 4 0.1789331248416275 1103 5 0.06107561565748654 1103 6 0.1854756159754558 1103 7 0.14128642972247007 1103 8 0.22127782786471073 1103 9 0.16918448452605617 1103 10-11 0.2447819872334449 1103 12-13 0.30807140748721196 1103 14-15 0.38850978769704625 1103 16-17 0.24480266186077415 1103 18-19 0.30506676858144033 1103 20-21 0.19933179104724275 1103 22-23 0.25855962194582105 1103 24-25 0.34142712246569573 1103 26-27 0.2949378481270273 1103 28-29 0.2729762085885383 1103 30-31 0.34891924687403986 1103 32-33 0.4093236795218118 1103 34-35 0.47999279772652415 1103 36-37 0.38433547278357594 1103 38-39 0.49736205940190814 1103 40-41 0.697421624155858 1103 42-43 0.7065186844993789 1103 44-45 0.8697585906695195 1103 46-47 0.9066308787422557 1103 48-49 0.9354903898235278 1103 50-51 0.7463515328203911 1103 52-53 0.9915333673248838 1103 54-55 1.0902201620664584 1103 56-57 0.8874960394045104 1103 58-59 0.8325335043143269 1103 60-61 1.0042977791996108 1103 62-63 0.8392682912787208 1103 64-65 0.7501346150897987 1103 66-67 0.6711658951043304 1103 68-69 0.8827818271592669 1103 70-71 0.9290205051952434 1103 72-73 0.6091729001454631 1103 74-75 0.7047553458178619 1103 76-77 0.7085622549023078 1103 78-79 0.8502369134775449 1103 80-81 0.8860694467452106 1103 82-83 0.8822909932558787 1103 84-85 1.0004301458024365 1103 86-87 0.8766645880088468 1103 88-89 0.802879205918618 1103 90-91 0.4395279931877951 1103 92-93 0.5902163919173518 1103 94-95 0.6458273856520869 1103 96-97 0.43680040948979126 1103 98-99 0.6028314661387775 1103 100-101 0.566384402441745 1104 1 0.34814389980565963 1104 2 0.35658873715821215 1104 3 0.16467112806592255 1104 4 0.09427454373915367 1104 5 0.00949927487542368 1104 6 0.12313028849813179 1104 7 0.1111305729517511 1104 8 -0.10706382825659944 1104 9 0.04172459742062529 1104 10-11 0.002801572787781481 1104 12-13 -0.01638075151108609 1104 14-15 0.16445379989199438 1104 16-17 -0.029550940760216804 1104 18-19 0.11608959220294679 1104 20-21 0.10969412978933235 1104 22-23 0.13117400758487463 1104 24-25 0.20869125131188326 1104 26-27 0.28078505506024953 1104 28-29 0.09259165911540634 1104 30-31 0.0981303094345094 1104 32-33 0.20420611905031905 1104 34-35 0.19259207438108206 1104 36-37 0.2281434457475484 1104 38-39 0.14914337050854698 1104 40-41 0.31859339736993064 1104 42-43 0.16713355057270718 1104 44-45 0.21224667896765226 1104 46-47 0.24173996052159907 1104 48-49 0.33564350018646394 1104 50-51 0.23967727549170803 1104 52-53 0.21537420738142998 1104 54-55 0.4162348549739221 1104 56-57 0.3816384080951636 1104 58-59 0.5158406956371735 1104 60-61 0.15554758182494055 1104 62-63 -0.04433677677905479 1104 64-65 0.21750025625627956 1104 66-67 0.19220884158828966 1104 68-69 0.16641540697085233 1104 70-71 0.10527159870706626 1104 72-73 0.30979200182316546 1104 74-75 0.16119877767514623 1104 76-77 0.3673016312806574 1104 78-79 0.3427116064897717 1104 80-81 0.4404167034430415 1104 82-83 0.4326475398464069 1104 84-85 0.3370903493060169 1104 86-87 0.296968623581936 1104 88-89 0.4521842599824417 1104 90-91 0.36050029637624803 1104 92-93 0.44862260733001236 1104 94-95 0.5173731417785596 1104 96-97 0.20599663995156803 1104 98-99 0.3637057816883136 1104 100-101 0.44103240201668825 1105 1 0.30811294201689776 1105 2 0.33305190170970533 1105 3 0.08167750621604952 1105 4 0.08542978951255975 1105 5 -0.08598178875624995 1105 6 -0.08016669762142214 1105 7 0.003652447000980885 1105 8 -0.04271979236467871 1105 9 -0.0552809167057049 1105 10-11 -0.13353839483890084 1105 12-13 0.029060925503948454 1105 14-15 0.1277828835763799 1105 16-17 0.05399809213059825 1105 18-19 -0.051100391003743084 1105 20-21 -0.14045178912294887 1105 22-23 -0.10739514143441653 1105 24-25 -0.07375054427662775 1105 26-27 -0.07227414068066906 1105 28-29 0.008796287525427715 1105 30-31 0.09670237223617306 1105 32-33 0.1024312395310858 1105 34-35 -0.0713161809986147 1105 36-37 -0.05526463177193364 1105 38-39 -0.20203036905078164 1105 40-41 -0.26710880120295855 1105 42-43 -0.26716433358009084 1105 44-45 -0.6045120276806415 1105 46-47 -0.2869109306637938 1105 48-49 0.0802720756593942 1105 50-51 -0.21952632436232733 1105 52-53 -0.33682064285292057 1105 54-55 -0.01811772004678147 1105 56-57 0.06332928427963225 1105 58-59 0.010658621005738667 1105 60-61 -0.05262539390058407 1105 62-63 -0.14922053340745123 1105 64-65 -0.013783287859027382 1105 66-67 -0.06882423673546612 1105 68-69 -0.06527900803448361 1105 70-71 -0.12116215226840765 1105 72-73 0.09338069708289254 1105 74-75 -0.05786043828760512 1105 76-77 -0.09912966549957147 1105 78-79 -0.20893449671695308 1105 80-81 -0.28894921339190915 1105 82-83 -0.11106792792596565 1105 84-85 0.031402407125753484 1105 86-87 -0.009623326473811034 1105 88-89 -0.12629345877413556 1105 90-91 -0.38134685781431443 1105 92-93 -0.1581197196331381 1105 94-95 -0.09012359745078413 1105 96-97 -0.06352685092516808 1105 98-99 -0.029505932636009646 1105 100-101 -0.08593685987720079 1106 1 0.2891719987279231 1106 2 0.35046853659688537 1106 3 0.0826079210393722 1106 4 0.03492503531974478 1106 5 -0.14011023470889228 1106 6 -0.13275865841669798 1106 7 -0.17036113893730231 1106 8 -0.1310687958909611 1106 9 -0.11120045270489953 1106 10-11 -0.09489186336005417 1106 12-13 -0.08014160216911392 1106 14-15 -0.03982683590992053 1106 16-17 -0.1921281775037258 1106 18-19 -0.18835373559872437 1106 20-21 -0.1033766126015081 1106 22-23 -0.08848906794548128 1106 24-25 -0.2038970915299103 1106 26-27 -0.11610963049896128 1106 28-29 -0.2316597651799981 1106 30-31 -0.3202640547882538 1106 32-33 -0.12361998807959651 1106 34-35 -0.42965595786510136 1106 36-37 -0.35257617018876175 1106 38-39 -0.3106273798786816 1106 40-41 -0.3520942527474986 1106 42-43 -0.37897600787844254 1106 44-45 -0.3357151298751688 1106 46-47 -0.3691820726940236 1106 48-49 -0.5373135947346412 1106 50-51 -0.6127323035921037 1106 52-53 -0.45830591921821906 1106 54-55 -0.5746685101244964 1106 56-57 -0.5755236432061324 1106 58-59 -0.788888182291501 1106 60-61 -0.8475342370479169 1106 62-63 -0.9788981648735202 1106 64-65 -0.5832035248518999 1106 66-67 -0.7696355407497535 1106 68-69 -0.6029583176788655 1106 70-71 -0.729318862684682 1106 72-73 -0.4661664050983525 1106 74-75 -0.38902916882209126 1106 76-77 -0.3060058240210033 1106 78-79 -0.2877939332320558 1106 80-81 -0.4193999027899977 1106 82-83 -0.4249954795512565 1106 84-85 -0.3884286016464955 1106 86-87 -0.46282473388535905 1106 88-89 -0.637611753076353 1106 90-91 -0.6771567808738226 1106 92-93 -0.4774626065536971 1106 94-95 -0.46870252579666527 1106 96-97 -0.5476987291509694 1106 98-99 -0.44571850779287914 1106 100-101 -0.41147688514950076 1107 1 0.37443917928365167 1107 2 0.4579432758737596 1107 3 0.29691518529467587 1107 4 0.10415450166436102 1107 5 -0.02170814857101533 1107 6 0.10312718269878474 1107 7 -0.026787428085469855 1107 8 0.004125253393695516 1107 9 0.09602481101758542 1107 10-11 0.15071673172560196 1107 12-13 0.22705604986717987 1107 14-15 0.2095750579165312 1107 16-17 0.25914116611444626 1107 18-19 0.32316510121708575 1107 20-21 0.16362662846351128 1107 22-23 0.10814274742143937 1107 24-25 0.05346940640847464 1107 26-27 0.16544929178075307 1107 28-29 0.2936657968898331 1107 30-31 0.19390832682113057 1107 32-33 0.27676975131196 1107 34-35 0.31681892079344465 1107 36-37 0.4551737155711919 1107 38-39 0.5443724525104443 1107 40-41 0.667383454124014 1107 42-43 0.6605417844569743 1107 44-45 0.4837287019564229 1107 46-47 0.3921837089284139 1107 48-49 0.3695494530662131 1107 50-51 0.1352916524126826 1107 52-53 0.17687401546646697 1107 54-55 0.1533846101724734 1107 56-57 0.49291099472791444 1107 58-59 0.4635651116150399 1107 60-61 0.23735096714623438 1107 62-63 0.3078769842560547 1107 64-65 0.37960979721328414 1107 66-67 0.3871167956576258 1107 68-69 0.2987612816782139 1107 70-71 0.17119658486281963 1107 72-73 0.474808102169785 1107 74-75 0.6263332499969039 1107 76-77 0.7843951011910271 1107 78-79 0.91443652642754 1107 80-81 0.5852407130300001 1107 82-83 0.7175262525218784 1107 84-85 0.8759890211372934 1107 86-87 0.6555910257712334 1107 88-89 0.7559072637006103 1107 90-91 0.6861543454888981 1107 92-93 0.56381333563996 1107 94-95 0.7356265984453323 1107 96-97 0.7384800781300456 1107 98-99 1.0081730638501831 1107 100-101 0.7170881540449798 1108 1 0.17151681619110448 1108 2 0.1026397150269105 1108 3 0.03755868135626628 1108 4 0.06613239597960785 1108 5 -0.16877255204777697 1108 6 -0.13194187707182436 1108 7 -0.10281055965387509 1108 8 0.08295316960851551 1108 9 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-1.1524107602035372 2316 74-75 -1.189734092271813 2316 76-77 -1.0138191845232605 2316 78-79 -0.8985701751089117 2316 80-81 -0.6973393785031803 2316 82-83 -0.5777818547027067 2316 84-85 -0.9322497823932707 2316 86-87 -1.0704632403288024 2316 88-89 -0.9141523963190039 2316 90-91 -1.0420934479076358 2316 92-93 -1.370122591133498 2316 94-95 -1.551168105706349 2316 96-97 -1.6276689901929338 2316 98-99 -2.1370149060746293 2316 100-101 -2.8976364291521435 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 14.0 5 44.0 6 99.0 7 225.0 8 565.0 9 1060.0 10 1733.0 11 2727.0 12 4049.0 13 5429.0 14 7296.0 15 9489.0 16 12054.0 17 15509.0 18 13206.0 19 8302.0 20 7376.0 21 7677.0 22 8198.0 23 8991.0 24 10577.0 25 12792.0 26 15654.0 27 19367.0 28 23997.0 29 29381.0 30 36839.0 31 44866.0 32 55955.0 33 70587.0 34 92298.0 35 124659.0 36 174734.0 37 230160.0 38 153065.0 39 10527.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.4571738824095 19.261018410298494 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27.59552244191081 30.776200816069483 20.76512254162348 20-21 21.902848167307432 25.71329233577435 30.77387641773886 21.609983079179354 22-23 21.254750980946874 25.86038368655621 31.016330664173815 21.868534668323104 24-25 22.036090082525355 25.95280540285231 30.93155086002178 21.07955365460056 26-27 21.616346987256286 25.763979260403623 31.556707937577848 21.062965814762244 28-29 20.74847642976161 27.19855777430824 31.167712447027647 20.885253348902506 30-31 21.165047906791575 26.254619195434863 30.826315791631593 21.75401710614197 32-33 21.15523234788342 26.338541448841806 30.92215052282066 21.58407568045411 34-35 22.249218836165312 25.887242623890472 30.612192182908576 21.25134635703564 36-37 21.958399790071876 25.54152845715129 31.2874498243082 21.212621928468632 38-39 21.50045674381838 26.37400348666915 31.14463936918513 20.980900400327346 40-41 21.44768721270312 26.584312498641804 30.781168700441224 21.186831588213852 42-43 21.218906921072726 26.825891879612552 29.90524560774765 22.049955591567073 44-45 21.733960707649363 26.302323788781063 29.704979359953555 22.258736143616016 46-47 22.260162201612175 26.91841537717205 29.344961335662212 21.47646108555356 48-49 22.23769020666093 26.945511370882123 29.118528544992085 21.69826987746486 50-51 21.343773690578548 27.261288144265862 29.7413649955289 21.653573169626693 52-53 22.143276527420838 27.744338952695998 28.28043074309087 21.83195377679229 54-55 21.383676940576546 27.056128302635713 29.100795740740892 22.45939901604685 56-57 22.340394998392714 27.029032808287027 28.55476412278342 22.075808070536834 58-59 22.8999605572498 26.873592135722426 28.254867407578093 21.971579899449686 60-61 21.95976518277389 27.023293517842344 29.733647199295728 21.283294100088035 62-63 21.7045424259757 27.233808753554378 29.278587229052498 21.783061591417425 64-65 21.402508037530346 27.765156485794897 28.933673971524176 21.898661505150578 66-67 21.607873928579483 27.212123324319837 28.509246863897562 22.67075588320312 68-69 21.6226610348642 27.15138872147096 28.806284199382866 22.419666044281975 70-71 22.280229309537535 27.098033845379245 28.439048550743056 22.18268829434016 72-73 21.896689145751058 26.721929571366722 29.217411341004212 22.16396994187801 74-75 21.37701065351159 28.114544929741932 28.34443347459295 22.16401094215353 76-77 21.74545168285582 27.153616328799878 28.313158907887274 22.787773080457026 78-79 21.60737402320805 27.336683994041145 28.561628360416496 22.49431362233431 80-81 22.146724087167406 27.442510471094693 28.065527278155084 22.345238163582817 82-83 21.78964409522177 27.13313645135126 28.73604883941598 22.341170614010988 84-85 21.51230161235959 27.470183935777882 28.339181871636132 22.67833258022639 86-87 21.635740657819415 27.515618823614318 28.034004668014912 22.814635850551355 88-89 21.389375565967164 27.34162762313477 27.63072201005289 23.638274800845178 90-91 21.471521102755002 26.76638224542139 28.287214331353074 23.474882320470538 92-93 21.792070548883466 26.921772758825274 28.61509520761743 22.671061484673828 94-95 21.21510084767898 26.986026192636718 28.532088009686824 23.26678494999748 96-97 20.590326556822067 27.146886191843418 29.094179810867843 23.168607440466673 98-99 20.882987072737276 27.51080278932982 27.63832859997983 23.967881537953076 100-101 20.219134043277336 26.829404311331086 28.50300776236422 24.448453883027362 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 289.0 1 230.0 2 193.5 3 242.0 4 223.5 5 223.0 6 377.0 7 674.5 8 1037.5 9 1295.5 10 1305.5 11 1247.5 12 1347.5 13 1535.5 14 1718.5 15 1852.0 16 1997.0 17 2158.5 18 2305.5 19 2492.5 20 2814.5 21 3229.5 22 3714.0 23 4380.5 24 5195.0 25 6382.5 26 8095.5 27 10009.0 28 11864.5 29 14054.0 30 16454.5 31 18870.5 32 21962.0 33 23831.5 34 25437.0 35 27797.5 36 30266.0 37 32512.5 38 33860.0 39 34628.5 40 35614.0 41 37605.0 42 38928.5 43 39779.0 44 41309.5 45 43041.5 46 43663.5 47 51064.5 48 68821.5 49 65494.0 50 47154.0 51 40145.0 52 36637.0 53 35143.0 54 33706.5 55 30932.0 56 27922.5 57 24964.0 58 22644.5 59 20053.5 60 16798.5 61 13730.0 62 11013.0 63 8439.5 64 6149.5 65 4726.0 66 3712.0 67 2940.0 68 2175.0 69 1485.0 70 1161.0 71 933.5 72 740.0 73 657.0 74 564.0 75 464.5 76 353.5 77 241.0 78 176.0 79 136.5 80 110.0 81 79.0 82 53.5 83 35.5 84 24.5 85 10.0 86 3.5 87 3.5 88 2.5 89 1.5 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0753552263871213 2 0.011644078248205828 3 0.005986040226190321 4 0.01582610635143468 5 0.0 6 0.0 7 0.0 8 0.0 9 0.004838032511578478 10-11 4.92003306262218E-4 12-13 3.28002204174812E-4 14-15 4.10002755218515E-5 16-17 0.0 18-19 0.0 20-21 4.10002755218515E-5 22-23 0.0015580104698303572 24-25 0.0 26-27 8.2000551043703E-5 28-29 0.0 30-31 4.10002755218515E-5 32-33 0.005002033613665884 34-35 7.790052349151786E-4 36-37 0.0031980214907044177 38-39 1.64001102087406E-4 40-41 0.004551030582925517 42-43 0.011562077697162124 44-45 0.0024600165313110904 46-47 0.0027880187354859024 48-49 0.011439076870596572 50-51 4.5100303074036653E-4 52-53 0.0024600165313110904 54-55 0.011234075492987314 56-57 0.004592030858447369 58-59 0.0012300082656555452 60-61 0.007175048216324014 62-63 0.00463303113396922 64-65 0.018368123433789477 66-67 0.0029930201130951603 68-69 8.2000551043703E-5 70-71 0.0013940093677429512 72-73 0.0 74-75 0.0 76-77 0.0011890079901336937 78-79 0.011685078523727679 80-81 0.015990107453522088 82-83 0.013202088718036187 84-85 0.009102061165851034 86-87 0.011644078248205828 88-89 0.028864193967383458 90-91 0.024846166966242013 92-93 0.0030750206641388628 94-95 0.009471063645547697 96-97 0.011890079901336937 98-99 0.01172607879924953 100-101 0.01303808761594878 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1219504.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.09798678358556 #Duplication Level Percentage of deduplicated Percentage of total 1 78.93964591989858 45.07294859435293 2 12.47972066817353 14.251338515484239 3 3.812443577360699 6.530485589799205 4 1.6189217451914328 3.6974868964239884 5 0.8502760088357502 2.427452415745177 6 0.5071814412740677 1.737542353844796 7 0.33985705652199993 1.358360760512101 8 0.23731332405154906 1.0840090432211285 9 0.17243367232266665 0.8861053988992269 >10 0.9262583278922346 9.683412280296961 >50 0.07069447187216948 2.7754622075620956 >100 0.0390566210889592 4.089061147325946 >500 0.0031706428223489164 1.2550823793748418 >1k 0.0024500421809059807 3.0300710315591677 >5k 5.764805131543484E-4 2.1211813855983017 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9215 0.7556350778677233 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6003 0.4922493079153492 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5359 0.43944095304320446 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5200 0.4264028654272557 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4312 0.3535863761004474 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3650 0.299302011309516 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3386 0.2776538658339784 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3178 0.26059775121688816 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3051 0.25018368123433793 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2325 0.1906512811766095 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1971 0.16162308610713866 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1789 0.14669898581718468 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1702 0.13956493787638252 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1695 0.1389909340190766 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1660 0.136120914732547 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1517 0.12439483593329748 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1470 0.12054081003424343 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1457 0.1194748028706753 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1261 0.10340269486610949 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1254 0.10282869100880357 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 8.200055104370302E-5 0.0 0.0 0.0 0.0 12-13 8.200055104370302E-5 0.0 0.0 0.0 0.0 14-15 1.6400110208740604E-4 0.0 0.0 0.0 0.0 16-17 1.6400110208740604E-4 0.0 0.0 0.0 0.0 18-19 1.6400110208740604E-4 0.0 0.0 0.0 0.0 20-21 1.6400110208740604E-4 0.0 0.0 8.200055104370302E-5 0.0 22-23 1.6400110208740604E-4 0.0 0.0 1.64001102087406E-4 0.0 24-25 1.6400110208740604E-4 0.0 0.0 4.100027552185151E-4 0.0 26-27 1.6400110208740604E-4 0.0 0.0 4.510030307403666E-4 0.0 28-29 1.6400110208740604E-4 0.0 0.0 0.0012710085411773966 0.0 30-31 1.6400110208740604E-4 0.0 0.0 0.0035260236948792293 0.0 32-33 1.6400110208740604E-4 0.0 0.0 0.00656004408349624 0.0 34-35 1.6400110208740604E-4 0.0 0.0 0.01004506750285362 0.0 36-37 1.6400110208740604E-4 0.0 0.0 0.013776092575342107 0.0 38-39 1.6400110208740604E-4 0.0 0.0 0.021566144924493894 0.0 40-41 1.6400110208740604E-4 0.0 0.0 0.03587524108162007 0.0 42-43 1.6400110208740604E-4 0.0 0.0 0.05768738765924507 0.0 44-45 1.6400110208740604E-4 0.0 0.0 0.07872052900195489 0.0 46-47 1.6400110208740604E-4 0.0 0.0 0.12599384667864968 0.0 48-49 2.46001653131109E-4 0.0 0.0 0.16937213818076857 0.0 50-51 2.46001653131109E-4 0.0 0.0 0.2028283630065994 0.0 52-53 2.46001653131109E-4 0.0 0.0 0.240220614282528 0.0 54-55 2.46001653131109E-4 0.0 0.0 0.27010981513795773 0.0 56-57 3.280022041748121E-4 0.0 0.0 0.3538323777535785 0.0 58-59 3.280022041748121E-4 0.0 0.0 0.4556360618743358 0.0 60-61 3.280022041748121E-4 0.0 0.0 0.5572347446174839 0.0 62-63 3.280022041748121E-4 0.0 0.0 0.6712975111192747 0.0 64-65 3.280022041748121E-4 0.0 0.0 0.8226705283459506 0.0 66-67 3.280022041748121E-4 0.0 0.0 1.032632939293352 0.0 68-69 4.100027552185151E-4 0.0 0.0 1.2655965048085123 0.0 70-71 4.100027552185151E-4 0.0 0.0 1.4243905719046432 0.0 72-73 4.510030307403666E-4 0.0 0.0 1.555919455778743 0.0 74-75 5.330035817840696E-4 0.0 0.0 1.7336556501659692 0.0 76-77 5.740038573059211E-4 0.0 0.0 1.955426140463664 0.0 78-79 5.740038573059211E-4 0.0 0.0 2.1621905299203608 0.0 80-81 6.560044083496242E-4 0.0 0.0 2.389742059066637 0.0 82-83 6.560044083496242E-4 0.0 0.0 2.681049016649392 0.0 84-85 6.560044083496242E-4 0.0 0.0 3.1919534499271833 0.0 86-87 6.560044083496242E-4 0.0 0.0 3.8341407654259436 0.0 88-89 6.560044083496242E-4 0.0 0.0 4.468292026922422 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGATTCG 110 9.498226E-5 25.900602 9 GTACATG 5205 0.0 24.627613 1 TACATGG 5295 0.0 23.322945 2 TAAGGTG 545 0.0 22.653128 5 ACATGGG 5200 0.0 22.557884 3 GAGTACT 6330 0.0 22.316936 12-13 GTACTTT 6815 0.0 20.624203 14-15 CATGGGG 2075 0.0 20.598194 4 AGTACTT 6485 0.0 19.769928 12-13 GTAAGGT 545 0.0 19.170387 4 GTAGGAC 250 2.1769847E-7 18.996113 3 ATGGGAG 1405 0.0 18.588215 5 ACTTTTT 7730 0.0 18.21363 16-17 GTACTAG 215 2.1845266E-5 17.86419 1 ATTACGA 160 0.0011936914 17.806665 5 GGTAAGG 510 0.0 17.692457 3 GTGTAGG 570 0.0 17.687899 1 CATGGGA 3095 0.0 17.492382 4 ACGATTC 165 0.0014670974 17.26707 8 GTATAAG 425 4.9112714E-11 16.94471 1 >>END_MODULE