FastQCFastQC Report
Thu 26 May 2016
SRR936497_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936497_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1269580
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT100850.7943571889916351No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT51520.4058034940689047No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48120.3790229839789537No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT41050.32333527623308495No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39380.3101813198065502No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC33850.2666236078073063No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT30830.2428362135509381No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC25950.20439830495124373No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC25890.20392570771436222No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT24180.19045668646323982No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23330.18376155894075205No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG21200.16698435703145922No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA19440.15312150474960223No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC18670.1470565068762898No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA15850.12484443674285985No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA15650.12326911261992156No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA13230.10420769073236817No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAC459.595684E-442.2488061
ATGGGCG2059.513315E-1025.5028465
CGTACAC1151.2760481E-424.799193
GTACATG58650.024.3930111
TACATGG58700.023.9683252
ACATGGG59900.022.5359343
GAGTACT46750.021.24824312-13
AGTACTT48200.020.11599512-13
TACTGCG1200.004746455219.8041277
GTACTTT50700.019.73303814-15
TAATACC3707.2759576E-1219.2696424
CATGGGG25150.018.8993174
TAAGGTG5800.018.8473225
GTACCGT1300.00754128718.278576
TAGACTG4700.018.2022255
CATGGGA34900.017.9776344
GTATAGC5050.017.882541
ACTTTTT58800.017.66030716-17
TACTTTT57100.017.22995214-15
GTGTAGC7750.017.1720941
Produced by FastQC (version 0.11.5)