FastQCFastQC Report
Thu 26 May 2016
SRR936497_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936497_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1269580
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT96430.7595425258746987No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63130.49725105940547265No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55880.44014555994895954No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT53410.42069030703067156No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT46270.36445123584177447No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC38470.30301359504718095No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT35590.2803289276768695No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34230.2696167236408891No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC30470.24000063012964917No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT25010.19699428157343374No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC19700.15516942610942203No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA19570.15414546542951213No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG18710.14737157170087747No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC18520.14587501378408607No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA18080.14240930071362182No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15580.12271774917689314No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA14720.11594385544825848No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA14430.11365963546999797No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT12970.10215976937254839No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGAC650.005847356329.2276427
GTACATG56500.025.3215521
TACATGG55950.025.1318422
ACATGGG56300.023.9593053
GAACCGT1100.002879316721.5885986
ATAAGGG3151.4551915E-1121.1096843
AGTACTT69300.020.87267912-13
GAGTACT67350.020.84222212-13
GTACTTT71400.019.72613714-15
CATGGGA32600.018.793264
TAAGGTG4600.018.5842365
TACTTTT78650.018.51174514-15
CATGGGG23300.018.141124
TATAACG1600.001190389217.8141022
CATGGGC11950.017.486984
ACTTTTT81400.017.41709316-17
AGAGTAC115350.017.25556410-11
GTATAGT3059.2091796E-817.1421431
ATGGGAG13150.016.97475
GTGTACA9900.016.8037091
Produced by FastQC (version 0.11.5)