##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936497_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1269580 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.870160998125364 31.0 31.0 34.0 30.0 34.0 2 32.00334126246475 31.0 31.0 34.0 30.0 34.0 3 32.1125884150664 33.0 31.0 34.0 30.0 34.0 4 35.85971187321791 37.0 35.0 37.0 35.0 37.0 5 35.6290710313647 37.0 35.0 37.0 33.0 37.0 6 35.739134989524096 37.0 35.0 37.0 33.0 37.0 7 35.683633957686794 37.0 35.0 37.0 33.0 37.0 8 35.66482458765891 37.0 35.0 37.0 33.0 37.0 9 37.29431307991619 39.0 37.0 39.0 34.0 39.0 10-11 37.16309173112368 39.0 37.0 39.0 33.0 39.0 12-13 37.07441122260906 39.0 37.0 39.0 33.0 39.0 14-15 38.143223743285176 40.0 37.0 41.0 33.0 41.0 16-17 38.089183824571904 40.0 37.0 41.0 33.0 41.0 18-19 38.2729741331779 40.0 38.0 41.0 33.5 41.0 20-21 38.307582822665765 40.0 38.0 41.0 34.0 41.0 22-23 38.21913034231793 40.0 38.0 41.0 33.0 41.0 24-25 38.08669953843003 40.0 37.0 41.0 33.0 41.0 26-27 37.86135966225051 40.0 37.0 41.0 33.0 41.0 28-29 37.77834323161991 40.0 37.0 41.0 32.5 41.0 30-31 37.73133437829833 40.0 37.0 41.0 33.0 41.0 32-33 37.56430630602246 40.0 36.5 41.0 33.0 41.0 34-35 37.36422596449219 39.0 36.0 41.0 32.0 41.0 36-37 37.104883898612144 39.0 36.0 41.0 31.5 41.0 38-39 36.92307771073898 39.0 35.5 41.0 31.0 41.0 40-41 36.69489634367271 39.0 35.0 41.0 30.5 41.0 42-43 36.4282483183415 39.0 35.0 40.5 30.0 41.0 44-45 36.14498259266844 39.0 35.0 40.0 30.0 41.0 46-47 35.735935112399375 39.0 35.0 40.0 28.5 41.0 48-49 35.321585091132505 38.0 35.0 40.0 27.0 41.0 50-51 34.9878203815435 38.0 34.0 40.0 26.0 41.0 52-53 34.728536602656 38.0 34.0 40.0 25.5 41.0 54-55 34.481290663053926 38.0 34.0 40.0 24.0 41.0 56-57 34.16430079238803 38.0 33.5 40.0 23.5 41.0 58-59 33.834225098063925 37.5 33.0 40.0 23.0 41.0 60-61 33.45331251279951 37.0 33.0 40.0 22.0 41.0 62-63 33.07785291198664 36.5 32.0 39.0 21.5 40.5 64-65 32.68355282849446 36.0 31.5 39.0 20.0 40.0 66-67 32.20772696482301 35.5 31.0 39.0 18.5 40.0 68-69 31.924295436286016 35.0 31.0 38.0 18.0 40.0 70-71 31.47748743679012 35.0 31.0 37.5 15.5 40.0 72-73 31.01615337355661 34.5 30.0 37.0 12.5 39.0 74-75 30.501380771593755 34.0 29.5 36.5 8.0 39.0 76-77 29.060473148600323 32.5 28.0 35.0 7.5 37.5 78-79 29.845767497912696 34.0 29.0 36.0 7.0 37.5 80-81 29.623822051387073 34.0 29.0 35.0 4.0 37.0 82-83 29.265085697632287 34.0 29.0 35.0 2.0 37.0 84-85 28.83892350226059 34.0 29.0 35.0 2.0 36.0 86-87 28.47475936924022 34.0 29.0 35.0 2.0 36.0 88-89 28.064896658737535 33.0 27.5 35.0 2.0 35.5 90-91 27.548555427779263 33.0 26.5 35.0 2.0 35.0 92-93 27.07830699916508 33.0 26.0 34.5 2.0 35.0 94-95 26.487166622032483 32.0 25.0 34.0 2.0 35.0 96-97 25.734620110587755 32.0 23.5 34.0 2.0 35.0 98-99 24.810634225491896 32.0 18.5 34.0 2.0 35.0 100-101 21.689527639061737 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3486681364939841 1101 2 0.44652448259895294 1101 3 0.39650747185404356 1101 4 0.3499736735837402 1101 5 0.2528063540917529 1101 6 0.28755769178792434 1101 7 0.28166090865016713 1101 8 0.28917634559098104 1101 9 0.487498628584099 1101 10-11 0.4033159966177209 1101 12-13 0.43861832220037655 1101 14-15 0.5242564921391946 1101 16-17 0.5799484542798652 1101 18-19 0.5796201159152616 1101 20-21 0.4724734182197423 1101 22-23 0.4282839296756933 1101 24-25 0.40586323190672147 1101 26-27 0.4375072078621187 1101 28-29 0.4372999158989259 1101 30-31 0.5377574031114563 1101 32-33 0.52082527357841 1101 34-35 0.46936737116561034 1101 36-37 0.42308761767912273 1101 38-39 0.5489332588036575 1101 40-41 0.571725110801033 1101 42-43 0.5227661126474246 1101 44-45 0.6657271862684411 1101 46-47 0.6867620725382011 1101 48-49 0.6434384200868664 1101 50-51 0.8432805579424212 1101 52-53 0.7340467937587576 1101 54-55 0.8300235813954089 1101 56-57 0.8211249295735144 1101 58-59 0.8491533740037838 1101 60-61 0.7746321868166888 1101 62-63 0.8972908599508926 1101 64-65 0.8123913807236818 1101 66-67 0.5939111284139855 1101 68-69 0.7578458907245569 1101 70-71 0.7558946418665649 1101 72-73 0.7638881965224726 1101 74-75 0.8732262007642042 1101 76-77 0.7287292115759207 1101 78-79 0.46084235346129176 1101 80-81 0.6724006721798048 1101 82-83 0.7735304473790094 1101 84-85 0.8641162006033696 1101 86-87 0.6828639191777022 1101 88-89 0.5296681699906394 1101 90-91 0.5889890320321811 1101 92-93 0.6871924710884088 1101 94-95 0.6785555515534902 1101 96-97 0.7855119749931845 1101 98-99 1.0258325814006604 1101 100-101 1.1360495118170952 1102 1 0.3717656694427163 1102 2 0.3571906098912905 1102 3 0.3673953288963929 1102 4 0.3110038785459821 1102 5 0.20057439968061175 1102 6 0.29561547519395504 1102 7 0.3028172803025342 1102 8 0.44937286545403055 1102 9 0.3561768097200684 1102 10-11 0.4099895813463803 1102 12-13 0.3940478283211064 1102 14-15 0.6019055822381532 1102 16-17 0.5421186737817649 1102 18-19 0.3389260818643294 1102 20-21 0.29895405937165975 1102 22-23 0.31379088291429014 1102 24-25 0.4838890046190727 1102 26-27 0.11893660845309029 1102 28-29 0.3328574061667311 1102 30-31 0.43218191415690654 1102 32-33 0.5944624739911433 1102 34-35 0.5394514432072626 1102 36-37 0.6995023027314247 1102 38-39 0.7616038652480057 1102 40-41 0.6043704487307764 1102 42-43 0.5790770853972944 1102 44-45 0.7914324768270404 1102 46-47 0.6295740302325044 1102 48-49 0.6086930399452228 1102 50-51 0.5329192943994414 1102 52-53 0.6864393879147528 1102 54-55 0.6864896242639418 1102 56-57 0.5756741416179807 1102 58-59 0.43247292984075614 1102 60-61 0.6016680317582939 1102 62-63 0.5233945253512005 1102 64-65 0.5471323637920165 1102 66-67 0.821799419300909 1102 68-69 0.6407582775732017 1102 70-71 0.5849311551633143 1102 72-73 0.6215713492826609 1102 74-75 0.6341511691052766 1102 76-77 0.4263149251916083 1102 78-79 0.5901693667345889 1102 80-81 0.8860538779393856 1102 82-83 0.7990232127277253 1102 84-85 0.666575337135427 1102 86-87 0.6568414530644837 1102 88-89 0.6809014024844267 1102 90-91 0.6815359927751103 1102 92-93 0.8106382522431375 1102 94-95 0.7943554085020388 1102 96-97 1.036289670692259 1102 98-99 1.0132654527481613 1102 100-101 1.1606285653324058 1103 1 0.13922510300584534 1103 2 0.2959785320435486 1103 3 0.19568979643080553 1103 4 0.17970737024604233 1103 5 0.05526660495176117 1103 6 0.15457120053677897 1103 7 0.011371696568922118 1103 8 0.12520099317608668 1103 9 0.194562178426672 1103 10-11 0.1615503314521618 1103 12-13 0.1622041086526096 1103 14-15 0.13959923719945522 1103 16-17 0.25175615791605566 1103 18-19 0.055012976167603256 1103 20-21 0.17175417012051497 1103 22-23 0.15334766970406832 1103 24-25 -0.014358612413850835 1103 26-27 0.2826425577823741 1103 28-29 0.23024531960840733 1103 30-31 0.16909674952685094 1103 32-33 0.1092295591806618 1103 34-35 0.36627848832809207 1103 36-37 0.4659170883266057 1103 38-39 0.5233420001874123 1103 40-41 0.5308678915520062 1103 42-43 0.3359153609504162 1103 44-45 0.34625534086725906 1103 46-47 0.391779581363636 1103 48-49 0.6451077944311692 1103 50-51 0.6770598707912612 1103 52-53 0.6139247043578493 1103 54-55 0.5110904928336595 1103 56-57 0.49526626849386446 1103 58-59 0.5842279570924376 1103 60-61 0.6003463560906894 1103 62-63 0.6878620286487873 1103 64-65 0.4492711234047704 1103 66-67 0.7368355993362457 1103 68-69 0.6506740337763866 1103 70-71 0.6788527225707917 1103 72-73 0.5385492119540736 1103 74-75 0.5343762443896161 1103 76-77 0.5159225755736294 1103 78-79 0.4684636787228449 1103 80-81 0.5308536589304005 1103 82-83 0.6326160488940715 1103 84-85 0.5369903031641492 1103 86-87 0.44421794941847637 1103 88-89 0.38747742722248546 1103 90-91 0.5655466259101729 1103 92-93 0.667567211203842 1103 94-95 0.7558585132032682 1103 96-97 0.6677023719689856 1103 98-99 1.0998690402646467 1103 100-101 1.2059908012150373 1104 1 0.15079649479735124 1104 2 0.14138430920507972 1104 3 0.19896927991215563 1104 4 0.05811866332452098 1104 5 0.047084679319333134 1104 6 0.12903379410686 1104 7 0.10809610533168978 1104 8 0.04637983607975826 1104 9 0.07444859631585388 1104 10-11 0.10138784275841317 1104 12-13 0.10946152275274557 1104 14-15 0.12415649086057812 1104 16-17 0.20407706023109284 1104 18-19 0.044323231231004456 1104 20-21 0.02413188491615159 1104 22-23 0.04581049966548534 1104 24-25 0.06605050886383879 1104 26-27 0.06427195115292506 1104 28-29 0.0777852249304587 1104 30-31 -0.1040741546271633 1104 32-33 -0.11712568272587731 1104 34-35 -0.06903705661643755 1104 36-37 0.2231214000760744 1104 38-39 0.19385318039081056 1104 40-41 0.29164414072133127 1104 42-43 0.1719577914104775 1104 44-45 0.2715658544613717 1104 46-47 0.3668161916689314 1104 48-49 0.29840204344409216 1104 50-51 0.20676215696691003 1104 52-53 0.19688413824700746 1104 54-55 0.24610847750107467 1104 56-57 0.21433890741037942 1104 58-59 0.0035426293172804435 1104 60-61 -0.011806872632924126 1104 62-63 0.17878167309049076 1104 64-65 0.3306506989787579 1104 66-67 0.10919177466720242 1104 68-69 0.35174379386059584 1104 70-71 0.13679756054452596 1104 72-73 0.0030107021265131095 1104 74-75 0.08911994368554943 1104 76-77 -0.031787410103628844 1104 78-79 0.1995415696693037 1104 80-81 0.3583286887560604 1104 82-83 0.15090673953886835 1104 84-85 0.148526129128701 1104 86-87 0.22195509867461283 1104 88-89 0.304735280539159 1104 90-91 0.30153195822033396 1104 92-93 0.24735643011614883 1104 94-95 0.3153528928384759 1104 96-97 0.4938120811183637 1104 98-99 0.5424530832778736 1104 100-101 1.005316514558043 1105 1 0.02922614375101773 1105 2 0.14090273110340945 1105 3 0.0748393341664908 1105 4 0.07388266642477248 1105 5 0.0972311849243539 1105 6 0.08556936188697506 1105 7 0.05673691139607229 1105 8 0.051876159261667 1105 9 -0.08639565404804728 1105 10-11 0.06138944959407411 1105 12-13 -0.0916405774759923 1105 14-15 0.049575213331699786 1105 16-17 0.11755510329428631 1105 18-19 -0.0156358417193303 1105 20-21 0.050115126565337675 1105 22-23 0.03800860359940117 1105 24-25 -0.08169070552585111 1105 26-27 0.07199161504070872 1105 28-29 0.05677868416409382 1105 30-31 0.22794937133740945 1105 32-33 0.2762888903284306 1105 34-35 0.14949755651424823 1105 36-37 -0.017658435081486346 1105 38-39 0.271748792784237 1105 40-41 0.07811056730784571 1105 42-43 -0.0677690937858415 1105 44-45 -0.09168675900959045 1105 46-47 0.05533984050917695 1105 48-49 0.03454119541294176 1105 50-51 0.178423246968606 1105 52-53 0.08966731940355288 1105 54-55 -0.04501564582828621 1105 56-57 -0.05290787419230014 1105 58-59 -0.004393012188231182 1105 60-61 0.07427178669900769 1105 62-63 0.12556020387557965 1105 64-65 -0.05253825544382096 1105 66-67 0.18616865513753567 1105 68-69 0.22020491788940788 1105 70-71 0.339558954899811 1105 72-73 0.11162405020492727 1105 74-75 -0.3772371708156719 1105 76-77 -0.39135635280576153 1105 78-79 -0.03772094947094473 1105 80-81 0.08788194118049475 1105 82-83 0.13544449072509224 1105 84-85 -0.06655213322030207 1105 86-87 0.06843565596303236 1105 88-89 0.09334376085069707 1105 90-91 0.19677808763676907 1105 92-93 0.24059543274379536 1105 94-95 0.14388604091310597 1105 96-97 0.41246720692414485 1105 98-99 0.7236162432619935 1105 100-101 0.5199994480728556 1106 1 0.0017638735271425787 1106 2 0.11235386716902696 1106 3 0.035308303958828446 1106 4 -0.08228880438084474 1106 5 -0.04989467636358569 1106 6 0.04740625958146438 1106 7 -0.028057929220949518 1106 8 -0.0857808991270943 1106 9 0.010509669843905556 1106 10-11 0.025373308640880055 1106 12-13 0.04662325677524848 1106 14-15 0.042534109929462716 1106 16-17 0.003250570952765486 1106 18-19 -0.00913475691376675 1106 20-21 -0.0060691592511332715 1106 22-23 -0.022934521594315527 1106 24-25 -0.09794103335170945 1106 26-27 -0.07691076437898658 1106 28-29 -0.07619165653637339 1106 30-31 0.046134467777811494 1106 32-33 -0.03331670536095288 1106 34-35 0.1482527123209323 1106 36-37 -0.04557817139028941 1106 38-39 0.04261163044488114 1106 40-41 0.09958882351269693 1106 42-43 0.11000937938012356 1106 44-45 0.1795003282278529 1106 46-47 0.07305763608889038 1106 48-49 0.29508705527036483 1106 50-51 0.20277057548587862 1106 52-53 -0.0036971871887274688 1106 54-55 -0.10991384099791901 1106 56-57 0.16480992138741613 1106 58-59 0.16909571060222106 1106 60-61 0.2430705135425839 1106 62-63 0.10041685456818783 1106 64-65 0.1290906814467263 1106 66-67 0.010418231944150591 1106 68-69 0.16561118353564197 1106 70-71 0.2180756322953208 1106 72-73 -0.021327515014537113 1106 74-75 0.07149146089879821 1106 76-77 -0.019745178116298945 1106 78-79 0.1721030974498774 1106 80-81 0.02713074312599062 1106 82-83 0.29882544821501966 1106 84-85 0.030799629886768543 1106 86-87 0.1424390653519616 1106 88-89 0.14669712136377555 1106 90-91 0.3122651772583964 1106 92-93 0.24697432791104745 1106 94-95 0.503093179172172 1106 96-97 0.5198465670080665 1106 98-99 0.6577303981865334 1106 100-101 0.8782627458817878 1107 1 -0.047653626520272496 1107 2 0.052171906649398636 1107 3 -0.03560803226591247 1107 4 -0.06586644564354316 1107 5 -0.034779696961841466 1107 6 0.010686493300859468 1107 7 -0.046569217221772874 1107 8 -0.07623160163910825 1107 9 -0.02945467316691719 1107 10-11 0.048778930437421764 1107 12-13 -0.10061813358571925 1107 14-15 -0.07848153872755148 1107 16-17 0.03321859607747513 1107 18-19 -0.0827086899019065 1107 20-21 -0.09283526261323516 1107 22-23 -0.032252297178956724 1107 24-25 0.09633298524721567 1107 26-27 -0.034770834895063274 1107 28-29 0.012652257805747524 1107 30-31 -0.1231227589429551 1107 32-33 -0.003988635553824338 1107 34-35 -0.19801151172425335 1107 36-37 -0.12800095000629597 1107 38-39 -0.13947315235292024 1107 40-41 -0.3477386354428518 1107 42-43 -0.4571035228646636 1107 44-45 -0.47758564815036664 1107 46-47 -0.2556809030863789 1107 48-49 -0.5278539530794646 1107 50-51 -0.44039334790783613 1107 52-53 -0.5633677066676341 1107 54-55 -0.493763095386214 1107 56-57 -0.4486335221935036 1107 58-59 -0.45585809947002787 1107 60-61 -0.36008361268132916 1107 62-63 -0.4525182741717302 1107 64-65 -0.3435200811825041 1107 66-67 -0.35819014483223555 1107 68-69 -0.24840197397184838 1107 70-71 -0.5106356343845562 1107 72-73 -0.6111155024286425 1107 74-75 -0.4256181124011036 1107 76-77 -0.500544556538852 1107 78-79 -0.5204799610329474 1107 80-81 -0.32953757820287066 1107 82-83 -0.6205584942367466 1107 84-85 -0.7232456267591836 1107 86-87 -0.572108816189921 1107 88-89 -0.7066033410914194 1107 90-91 -0.7353518148796709 1107 92-93 -0.861002844290887 1107 94-95 -0.5564105660543213 1107 96-97 -0.6938815046303048 1107 98-99 -0.2827655295786329 1107 100-101 -0.39700633292918397 1108 1 -0.11713749261262052 1108 2 -0.06621848101779904 1108 3 -0.11576672643957053 1108 4 -0.13399612145401818 1108 5 -0.218526390833226 1108 6 -0.10821851690090512 1108 7 -0.044475209816049244 1108 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-0.14317238529345744 2316 90-91 -0.30934716930905637 2316 92-93 -0.3450189164372901 2316 94-95 -0.615408767123494 2316 96-97 -0.60280449256528 2316 98-99 -0.870419298147727 2316 100-101 -0.9405162670653979 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 14.0 5 36.0 6 83.0 7 206.0 8 520.0 9 1022.0 10 1745.0 11 2797.0 12 4062.0 13 5589.0 14 7157.0 15 9771.0 16 12430.0 17 15880.0 18 13945.0 19 9126.0 20 7912.0 21 8205.0 22 8697.0 23 9839.0 24 11226.0 25 13397.0 26 16428.0 27 20400.0 28 24921.0 29 30951.0 30 37870.0 31 47296.0 32 58141.0 33 73502.0 34 96722.0 35 130399.0 36 184505.0 37 242068.0 38 153223.0 39 9488.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.52349733665332 19.240946197245247 15.181627635767642 22.053928830333785 2 15.488552059652488 21.344150309652886 39.15435065845546 24.012946972239156 3 20.47604381720409 24.514886018398688 30.593353377228254 24.415716787168968 4 12.605458353451581 16.95367827236946 38.13719057607376 32.30367279810519 5 12.896058047817615 38.119400680858305 34.541113790925145 14.443427480398938 6 30.84633700831064 37.11377884129911 16.940209219291148 15.0996749310991 7 27.425290253469655 33.736196222372755 20.807904976448903 18.03060854770869 8 26.194174451393376 33.526993178846546 19.9234392476252 20.355393122134878 9 25.61839932953572 17.335581867217826 21.215326788324848 35.83069201492161 10-11 24.942707137475693 26.298950001713173 27.348633222101753 21.40970963870938 12-13 26.67822966412958 24.09971273670472 27.739890844673816 21.482166754491885 14-15 22.82188742319135 25.4502576107616 27.698257100344925 24.029597865702126 16-17 21.294919657306384 28.835738881421126 27.96781959945035 21.901521861822147 18-19 20.87710108854897 27.633114888388288 30.691370374454547 20.798413648608204 20-21 22.017202539422488 25.629538902629218 30.690425179980778 21.662833377967516 22-23 21.366485605991386 25.919258386396503 30.85972576239252 21.85453024521959 24-25 22.08253352121862 25.99646021602607 30.842632420923948 21.07837384183136 26-27 21.653507488463156 25.766126475410577 31.500500780019525 21.07986525610674 28-29 20.7615566697256 27.21139341711927 31.101649855652404 20.925400057502728 30-31 21.212313356272958 26.246401817316094 30.72569373198447 21.815591094426477 32-33 21.167718203801286 26.35171751065321 30.863914553746547 21.616649731798958 34-35 22.321109607844296 25.90968976905489 30.5185876644157 21.250612958685107 36-37 22.057831308217406 25.54769509391394 31.188715281265228 21.20575831660343 38-39 21.54116952463027 26.35619192186942 31.013903894122286 21.088734659378026 40-41 21.5016425027165 26.60369979595237 30.700445391756887 21.194212309574247 42-43 21.289489178443922 26.881489635290617 29.73240992538827 22.096611260877186 44-45 21.73094032295479 26.301387450834284 29.701979073827633 22.26569315238329 46-47 22.385924782939856 26.884660190504096 29.268363764681048 21.461051261875 48-49 22.293667912885706 27.0154898475748 28.998951222595622 21.691891016943877 50-51 21.451743059720922 27.288946161416895 29.633295223121962 21.62601555574022 52-53 22.18771722884425 27.786899611474215 28.192242481051245 21.833140678630294 54-55 21.411878132524855 27.058752637760204 29.074359828694263 22.455009401020682 56-57 22.33715249826318 27.10123648943494 28.467731390826128 22.093879621475754 58-59 22.8943164740021 26.811881874407277 28.296643572123976 21.997158079466654 60-61 21.978295938975936 27.06198032030701 29.625444652045008 21.334279088672044 62-63 21.694057984942127 27.239027587195608 29.21594451997713 21.85096990788514 64-65 21.409772555581025 27.76404482875695 28.906032755840506 21.920149859821514 66-67 21.662149271742376 27.287310255628626 28.40511934665945 22.64542112596955 68-69 21.650960041842325 27.09877214208745 28.810038061389744 22.44022975468048 70-71 22.28397940102932 27.114245836017954 28.40510783898272 22.196666923970014 72-73 21.937889578409553 26.723715235536048 29.215126640182458 22.12326854587194 74-75 21.457691430605973 28.117082269758853 28.26032758553463 22.164898714100545 76-77 21.77765524846647 27.14724850585351 28.281480460403884 22.793615785276135 78-79 21.660040849795475 27.358886642211406 28.488203105246118 22.492869402747004 80-81 22.21939962087869 27.414141390271634 28.02902211829798 22.337436870551695 82-83 21.771683066617513 27.16862174245933 28.72468720362721 22.335007987295945 84-85 21.56547041859886 27.47761514993734 28.28143745121099 22.67547698025281 86-87 21.685286432999956 27.474598847289034 28.05646818623285 22.783646533478162 88-89 21.446064819738172 27.386075485699024 27.471032936077496 23.696826758485308 90-91 21.48712511097684 26.800897110387588 28.225812169285074 23.486165609350493 92-93 21.841361536146998 27.014034717375857 28.495249557722875 22.649354188754266 94-95 21.322310534319037 27.007826785937226 28.433252556712002 23.236610123031735 96-97 20.7343147984998 27.066564951997318 29.039012984373773 23.160107265129103 98-99 21.023300583912743 27.43956664584546 27.555553630097222 23.98157914014457 100-101 20.326729658953912 26.773927636458016 28.353421626511743 24.54592107807633 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 342.0 1 245.5 2 187.5 3 262.5 4 262.0 5 243.5 6 378.0 7 716.5 8 1094.5 9 1312.0 10 1305.0 11 1268.5 12 1342.0 13 1473.0 14 1705.5 15 1852.0 16 1992.5 17 2154.0 18 2363.5 19 2589.5 20 2906.0 21 3331.0 22 3846.0 23 4534.0 24 5408.5 25 6593.5 26 8224.0 27 10317.0 28 12285.5 29 14558.5 30 17119.0 31 19427.5 32 22610.0 33 24785.0 34 26456.5 35 28781.0 36 31374.0 37 34022.0 38 35386.0 39 35923.5 40 36928.0 41 38892.0 42 40273.5 43 41367.0 44 42815.5 45 44387.0 46 45247.0 47 52830.5 48 71159.0 49 68335.0 50 49450.5 51 41969.5 52 38439.5 53 36828.5 54 35200.5 55 32458.5 56 29592.5 57 26448.5 58 23890.0 59 21113.0 60 17629.0 61 14429.5 62 11666.0 63 9013.5 64 6517.5 65 4935.5 66 3929.5 67 3080.0 68 2249.5 69 1562.0 70 1179.5 71 968.5 72 804.0 73 723.0 74 608.0 75 523.0 76 418.0 77 270.5 78 183.5 79 122.0 80 91.5 81 82.5 82 64.0 83 40.5 84 22.5 85 11.0 86 8.5 87 5.0 88 3.5 89 2.5 90 2.5 91 1.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03938310307345737 2 0.007404023377809984 3 0.002599284802848186 4 2.362986184407442E-4 5 4.725972368814884E-4 6 2.362986184407442E-4 7 0.0 8 0.0 9 9.451944737629768E-4 10-11 5.119803399549457E-4 12-13 0.0010239606799098914 14-15 0.002284219978260527 16-17 0.003898927204272279 18-19 0.0 20-21 0.0 22-23 0.0018903889475259535 24-25 8.664282676160619E-4 26-27 0.004765355471888341 28-29 0.005907465461018605 30-31 0.0012208761952771782 32-33 7.876620614691472E-4 34-35 0.009648860252997054 36-37 0.011539249200523008 38-39 0.009097496809968651 40-41 0.001851005844452496 42-43 5.907465461018605E-4 44-45 0.0016934734321586665 46-47 7.876620614691474E-5 48-49 5.119803399549457E-4 50-51 0.001299642401424093 52-53 0.0029537327305093025 54-55 0.002914349627435845 56-57 0.001417791710644465 58-59 0.0012602592983506358 60-61 0.003780777895051907 62-63 0.006143764079459348 64-65 0.01000330818065817 66-67 0.003386946864317333 68-69 0.0040958427196395655 70-71 1.5753241229382947E-4 72-73 0.0014571748137179226 74-75 0.00626191338867972 76-77 0.003347563761243876 78-79 0.004017076513492651 80-81 0.005159186502622915 82-83 0.005041037193402543 84-85 0.006537595110193922 86-87 0.004017076513492651 88-89 0.009530710943776681 90-91 0.01346902125112242 92-93 0.0011027268860568063 94-95 0.0023236030813339843 96-97 0.0012208761952771782 98-99 0.003229414452023504 100-101 0.004686589265741426 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1269580.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.047840532738135 #Duplication Level Percentage of deduplicated Percentage of total 1 78.20877843024697 43.83433141718732 2 12.760238477828297 14.303676227300594 3 4.015374601414566 6.7515922601787155 4 1.6964279881662672 3.8032450142406695 5 0.9162071458700797 2.567571600334069 6 0.529707902329076 1.781339042320277 7 0.3586891251428598 1.407262562078405 8 0.2515137351086408 1.1277441373729953 9 0.1757587245512782 0.886580726929874 >10 0.9649745863224087 9.927742850099259 >50 0.07442278600996352 2.8587897446520785 >100 0.04186551696548366 4.344668474050476 >500 0.0030907339760170784 1.2429965644350516 >1k 0.002388294436013197 3.045522685874758 >5k 5.619516320031051E-4 2.1169366929454174 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9643 0.7595425258746987 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6313 0.49725105940547265 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5588 0.44014555994895954 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5341 0.42069030703067156 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4627 0.36445123584177447 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3847 0.30301359504718095 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3559 0.2803289276768695 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3423 0.2696167236408891 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3047 0.24000063012964917 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2501 0.19699428157343374 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1970 0.15516942610942203 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1957 0.15414546542951213 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1871 0.14737157170087747 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1852 0.14587501378408607 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1808 0.14240930071362182 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1558 0.12271774917689314 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1472 0.11594385544825848 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1443 0.11365963546999797 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1297 0.10215976937254839 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 7.876620614691472E-5 0.0 0.0 0.0 0.0 7 1.5753241229382944E-4 0.0 0.0 0.0 0.0 8 2.362986184407442E-4 0.0 0.0 0.0 0.0 9 2.362986184407442E-4 0.0 0.0 0.0 0.0 10-11 2.362986184407442E-4 0.0 0.0 0.0 0.0 12-13 2.362986184407442E-4 0.0 0.0 0.0 0.0 14-15 2.362986184407442E-4 0.0 0.0 0.0 0.0 16-17 2.362986184407442E-4 0.0 0.0 3.938310307345736E-5 0.0 18-19 2.756817215142015E-4 0.0 0.0 7.876620614691472E-5 0.0 20-21 3.150648245876589E-4 0.0 0.0 1.1814930922037208E-4 0.0 22-23 3.150648245876589E-4 0.0 0.0 2.756817215142015E-4 0.0 24-25 3.9383103073457366E-4 0.0 0.0 5.907465461018604E-4 0.0 26-27 3.9383103073457366E-4 0.0 0.0 6.695127522487751E-4 0.0 28-29 3.9383103073457366E-4 0.0 0.0 0.0018116227413790386 0.0 30-31 3.9383103073457366E-4 0.0 0.0 0.0036626285858315346 0.0 32-33 3.9383103073457366E-4 0.0 0.0 0.006261913388679721 0.0 34-35 5.119803399549457E-4 0.0 0.0 0.009727626459143969 0.0 36-37 6.301296491753178E-4 0.0 0.0 0.014532365034105769 0.0 38-39 6.301296491753178E-4 0.0 0.0 0.021936388411915753 0.0 40-41 6.695127522487751E-4 0.0 0.0 0.03564170828147892 0.0 42-43 7.876620614691472E-4 0.0 0.0 0.05982293356858173 0.0 44-45 8.66428267616062E-4 0.0 0.0 0.08171993887742403 0.0 46-47 0.0010239606799098914 0.0 0.0 0.12850706532869138 0.0 48-49 0.001102726886056806 0.0 0.0 0.1759637045322075 0.0 50-51 0.001102726886056806 0.0 0.0 0.2111721986798784 0.0 52-53 0.001102726886056806 0.0 0.0 0.24705020557979804 0.0 54-55 0.001102726886056806 0.0 0.0 0.2807227587076041 0.0 56-57 0.0012602592983506355 0.0 0.0 0.36094613966823674 0.0 58-59 0.0012602592983506355 0.0 0.0 0.4644449345452827 0.0 60-61 0.0012602592983506355 0.0 0.0 0.5656201263409946 3.938310307345736E-5 62-63 0.0012602592983506355 0.0 0.0 0.6792009956048457 7.876620614691472E-5 64-65 0.0012602592983506355 0.0 0.0 0.8272026969548985 7.876620614691472E-5 66-67 0.0012602592983506355 0.0 0.0 1.031246553978481 7.876620614691472E-5 68-69 0.0013390255044975503 0.0 0.0 1.2624647521227492 7.876620614691472E-5 70-71 0.0014571748137179224 0.0 0.0 1.4283463822681517 7.876620614691472E-5 72-73 0.00149655791679138 0.0 0.0 1.5589407520597363 7.876620614691472E-5 74-75 0.00149655791679138 0.0 0.0 1.7330534507474913 7.876620614691472E-5 76-77 0.00149655791679138 0.0 0.0 1.9604121047905607 7.876620614691472E-5 78-79 0.00149655791679138 0.0 0.0 2.160596417712944 7.876620614691472E-5 80-81 0.0015753241229382947 0.0 0.0 2.3711384867436474 7.876620614691472E-5 82-83 0.0015753241229382947 0.0 0.0 2.659737866065943 7.876620614691472E-5 84-85 0.0015753241229382947 0.0 0.0 3.1601395737172924 7.876620614691472E-5 86-87 0.0015753241229382947 0.0 0.0 3.7725074434064805 7.876620614691472E-5 88-89 0.0015753241229382947 0.0 0.0 4.371681973566061 7.876620614691472E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCGAC 65 0.0058473563 29.227642 7 GTACATG 5650 0.0 25.321552 1 TACATGG 5595 0.0 25.131842 2 ACATGGG 5630 0.0 23.959305 3 GAACCGT 110 0.0028793167 21.588598 6 ATAAGGG 315 1.4551915E-11 21.109684 3 AGTACTT 6930 0.0 20.872679 12-13 GAGTACT 6735 0.0 20.842222 12-13 GTACTTT 7140 0.0 19.726137 14-15 CATGGGA 3260 0.0 18.79326 4 TAAGGTG 460 0.0 18.584236 5 TACTTTT 7865 0.0 18.511745 14-15 CATGGGG 2330 0.0 18.14112 4 TATAACG 160 0.0011903892 17.814102 2 CATGGGC 1195 0.0 17.48698 4 ACTTTTT 8140 0.0 17.417093 16-17 AGAGTAC 11535 0.0 17.255564 10-11 GTATAGT 305 9.2091796E-8 17.142143 1 ATGGGAG 1315 0.0 16.9747 5 GTGTACA 990 0.0 16.803709 1 >>END_MODULE