FastQCFastQC Report
Thu 26 May 2016
SRR936496_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936496_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1371424
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT105310.7678879762932543No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT56690.41336596121987074No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48790.3557616025386751No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT44280.3228760762535875No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41560.3030426768089227No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC36920.2692092306974357No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT33140.2416466388221294No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC26580.19381314604382016No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC26540.193521478404928No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT25600.18666728889096296No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22580.16464638215460717No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG21950.16005261684205616No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA21370.15582343607812027No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC20260.14772965909886365No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA17060.1243962479874933No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA15490.1129482931609772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG60900.025.1290591
TACATGG61450.024.4409832
ACATGGG59850.023.745233
ATTCGCG1102.1791493E-923.74428480-81
GAGTACT50050.022.88999612-13
GTATAGG3850.022.2202741
GTACTTT53250.021.50934214-15
TCGCGCT1005.409911E-721.36751660-61
GTGTAGC7150.020.605981
GTATTAG4100.019.7061921
CATGGGA34900.019.475114
AGTACTT51150.019.14495512-13
CATGGGG25600.019.1234654
ACTTTTT62750.018.4017416-17
ATAGGAC4201.8189894E-1218.106733
AATTCGC1254.5689976E-617.0983878-79
TACTTTT59800.016.49098614-15
CAATTCG1306.635566E-616.44074878-79
ATAGCCC4055.0931703E-1016.430183
TAGCCCT8850.016.1119214
Produced by FastQC (version 0.11.5)