##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936496_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1371424 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.41912639708799 31.0 31.0 34.0 30.0 34.0 2 31.463519670065565 31.0 31.0 34.0 28.0 34.0 3 31.41595378234594 33.0 31.0 34.0 28.0 34.0 4 35.071056799356 37.0 35.0 37.0 32.0 37.0 5 35.1108089110297 37.0 35.0 37.0 32.0 37.0 6 35.178747054156844 37.0 35.0 37.0 32.0 37.0 7 35.040715344051144 37.0 35.0 37.0 32.0 37.0 8 35.03324646498822 37.0 35.0 37.0 32.0 37.0 9 36.52386060036867 39.0 37.0 39.0 32.0 39.0 10-11 36.41898311536039 39.0 37.0 39.0 32.0 39.0 12-13 36.341007230440766 39.0 37.0 39.0 32.0 39.0 14-15 37.329493285810955 40.0 37.0 41.0 32.0 41.0 16-17 37.27620123317078 40.0 37.0 41.0 32.0 41.0 18-19 37.17885642952143 39.5 37.0 41.0 31.0 41.0 20-21 37.18820838777796 39.5 36.5 41.0 31.0 41.0 22-23 37.18141690680636 39.0 36.5 41.0 31.0 41.0 24-25 37.28105020766736 40.0 37.0 41.0 31.0 41.0 26-27 37.2018081206104 39.5 36.5 41.0 31.0 41.0 28-29 37.11306969981567 39.0 36.0 41.0 31.0 41.0 30-31 36.929731797022654 39.0 36.0 41.0 30.5 41.0 32-33 36.77263778379262 39.0 36.0 41.0 30.0 41.0 34-35 36.568157623025414 39.0 35.0 41.0 30.0 41.0 36-37 36.347554075180255 39.0 35.0 40.5 30.0 41.0 38-39 36.16146392362974 39.0 35.0 40.0 30.0 41.0 40-41 35.98888308794363 38.5 35.0 40.0 29.5 41.0 42-43 35.697041906806355 38.0 35.0 40.0 28.0 41.0 44-45 35.540663573045244 38.0 35.0 40.0 27.0 41.0 46-47 35.39322230032434 38.0 35.0 40.0 26.5 41.0 48-49 35.145138556711856 38.5 34.5 40.0 26.0 41.0 50-51 34.133841175303914 37.5 33.0 39.5 24.0 40.5 52-53 34.24575696502322 38.0 33.5 39.5 24.0 40.5 54-55 34.7119045605152 38.0 34.0 40.0 24.5 41.0 56-57 34.56523657162191 38.0 34.0 40.0 24.0 41.0 58-59 34.34816949389831 38.0 34.0 40.0 23.5 41.0 60-61 34.02885577326924 38.0 33.5 40.0 23.0 41.0 62-63 33.73615198508995 37.0 33.0 40.0 22.0 41.0 64-65 33.389784632615445 37.0 33.0 39.5 22.0 41.0 66-67 33.00681226229088 36.0 32.0 39.0 20.5 41.0 68-69 32.5754912412208 36.0 32.0 39.0 19.5 40.5 70-71 32.16415783885947 35.0 31.0 38.5 18.0 40.0 72-73 31.752817509391697 35.0 31.0 37.5 17.0 40.0 74-75 31.351299452248174 35.0 31.0 37.0 13.5 39.0 76-77 30.90082935693119 35.0 30.5 36.5 9.5 39.0 78-79 30.43239545173484 34.5 30.0 36.0 7.0 39.0 80-81 29.952065517301726 34.0 29.5 36.0 6.0 37.5 82-83 29.54566421471405 34.0 29.0 35.0 2.0 37.0 84-85 29.171975989919964 34.0 29.0 35.0 2.0 36.5 86-87 28.86393230685769 34.0 29.0 35.0 2.0 36.0 88-89 28.501926100170337 34.0 29.0 35.0 2.0 36.0 90-91 28.13105283267611 34.0 27.5 35.0 2.0 35.5 92-93 27.716600044916817 34.0 27.0 35.0 2.0 35.0 94-95 27.241028668012227 33.5 26.0 35.0 2.0 35.0 96-97 26.6558022901743 33.0 25.0 35.0 2.0 35.0 98-99 25.96943942938143 33.0 24.5 35.0 2.0 35.0 100-101 23.540097008656694 31.0 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6117487831633603 1101 2 0.5915421022846168 1101 3 0.7042332251620671 1101 4 0.7421809477073964 1101 5 0.7483063460874462 1101 6 0.5906099962360258 1101 7 0.6965682825088635 1101 8 0.6298020266266633 1101 9 0.8461119332905938 1101 10-11 0.9247890219834147 1101 12-13 0.9064856104996508 1101 14-15 1.3024642931923438 1101 16-17 1.3698151828269758 1101 18-19 1.2684562848641932 1101 20-21 1.4017761183703215 1101 22-23 1.1589606962883252 1101 24-25 1.1126190257142525 1101 26-27 1.0160845293957053 1101 28-29 1.0089101416877995 1101 30-31 0.9784631521605576 1101 32-33 0.8751581954928511 1101 34-35 1.0639019386543325 1101 36-37 0.977180125515531 1101 38-39 0.9609772067650795 1101 40-41 0.9432579390067701 1101 42-43 1.1127240946414148 1101 44-45 1.078898813462466 1101 46-47 1.1832045691720765 1101 48-49 1.1875168171655375 1101 50-51 1.0429331927763386 1101 52-53 1.0751489105994168 1101 54-55 0.9551702167288241 1101 56-57 1.0705676738121 1101 58-59 0.8755814685166072 1101 60-61 0.7059183571475316 1101 62-63 0.812840869099162 1101 64-65 0.8210333066299924 1101 66-67 0.7264660794110682 1101 68-69 0.8381887658332658 1101 70-71 0.9105438714772447 1101 72-73 0.9344195973160794 1101 74-75 0.7490038626849689 1101 76-77 0.7523821961671189 1101 78-79 0.7577686621627393 1101 80-81 0.7760346269447105 1101 82-83 0.7374218711991709 1101 84-85 0.8005662389482708 1101 86-87 0.7894287119728318 1101 88-89 0.8049886083486548 1101 90-91 0.7067319508368861 1101 92-93 0.8174878433782773 1101 94-95 1.0503978847696622 1101 96-97 0.9262910526869312 1101 98-99 0.6751785989535293 1101 100-101 1.0437216171416352 1102 1 0.2756939055834202 1102 2 0.5727537258745059 1102 3 0.5313158490924685 1102 4 0.4609473555106476 1102 5 0.4625354127511798 1102 6 0.3777111287177064 1102 7 0.5859214718013632 1102 8 0.39073223236266585 1102 9 0.6525561495056209 1102 10-11 0.6355688800662023 1102 12-13 0.68446716236204 1102 14-15 0.835786550686386 1102 16-17 0.739967123204174 1102 18-19 0.7817566286728876 1102 20-21 0.8014340946222589 1102 22-23 0.4921181892309079 1102 24-25 0.7439323914146669 1102 26-27 0.5502573838866311 1102 28-29 0.40550844021919374 1102 30-31 0.3316753743949832 1102 32-33 0.49026023967171284 1102 34-35 0.34943327687202697 1102 36-37 0.2912043349202875 1102 38-39 0.32386193199272384 1102 40-41 0.5368604060813169 1102 42-43 0.30292431103219997 1102 44-45 0.3949994681269544 1102 46-47 0.35451782502946116 1102 48-49 0.5713856810590343 1102 50-51 0.5711463761373139 1102 52-53 0.41877279706565673 1102 54-55 0.6051514061091936 1102 56-57 0.6223733559922593 1102 58-59 0.6730877570303164 1102 60-61 0.6493544120251045 1102 62-63 0.6167639130693274 1102 64-65 0.6335896011850792 1102 66-67 0.49758038207256305 1102 68-69 0.6811777595612369 1102 70-71 0.49153512247956144 1102 72-73 0.5875372172372124 1102 74-75 0.6362554801236477 1102 76-77 0.6323193659514992 1102 78-79 0.5481727746859413 1102 80-81 0.7873204495042714 1102 82-83 0.7135614926800748 1102 84-85 0.6327359684048801 1102 86-87 0.601477394405638 1102 88-89 0.6009211207883745 1102 90-91 0.8079863582886375 1102 92-93 0.7505628167413398 1102 94-95 0.9534395756933307 1102 96-97 0.954240063550408 1102 98-99 1.073575851779239 1102 100-101 1.7090281066681001 1103 1 0.47860139716071615 1103 2 0.540718712220972 1103 3 0.47511054359049965 1103 4 0.552063538888909 1103 5 0.3774986214496039 1103 6 0.4326380957524165 1103 7 0.4809806379994086 1103 8 0.3542545554739718 1103 9 0.6746041769944 1103 10-11 0.5873812678705548 1103 12-13 0.5305264443151358 1103 14-15 0.7875589407475516 1103 16-17 0.9253669933572013 1103 18-19 1.00438141832052 1103 20-21 0.8479472317618004 1103 22-23 0.671247684705726 1103 24-25 0.5777466689137114 1103 26-27 0.5676746792816232 1103 28-29 0.6470471295593825 1103 30-31 0.6428107990716825 1103 32-33 0.693375666536376 1103 34-35 0.6595706121693965 1103 36-37 0.7196956544829192 1103 38-39 0.6468992016779112 1103 40-41 0.7935747954416001 1103 42-43 0.7242552399095885 1103 44-45 0.5550026255646898 1103 46-47 0.7817214026818959 1103 48-49 0.8503868923814153 1103 50-51 0.8013363018448203 1103 52-53 0.8570826354774255 1103 54-55 0.921247610482304 1103 56-57 0.873286880606635 1103 58-59 0.8348237968246295 1103 60-61 0.9003505809829946 1103 62-63 0.9187811767310095 1103 64-65 0.9310140114104044 1103 66-67 0.7543862894478579 1103 68-69 0.9137471536589956 1103 70-71 0.8876506529145303 1103 72-73 0.9720108879943297 1103 74-75 0.8979181995516647 1103 76-77 0.8757809931497498 1103 78-79 0.9494403323045475 1103 80-81 1.0478013813086484 1103 82-83 0.989926428910163 1103 84-85 1.0224072266888484 1103 86-87 0.9135971344044123 1103 88-89 0.9100921529923873 1103 90-91 0.9948415105674648 1103 92-93 1.2066056943220183 1103 94-95 1.1900217235085684 1103 96-97 1.2533725893178271 1103 98-99 1.323000586224687 1103 100-101 1.68064931055779 1104 1 0.1646729226308885 1104 2 0.2757545340546983 1104 3 0.452637480481215 1104 4 0.43172366799257134 1104 5 0.47714671592146374 1104 6 0.3716281537496684 1104 7 0.5067025144348989 1104 8 0.4295875897323782 1104 9 0.5542911691154799 1104 10-11 0.4286001504227528 1104 12-13 0.5207595884432834 1104 14-15 0.6573127949189725 1104 16-17 0.5391688087599178 1104 18-19 0.5049161266821756 1104 20-21 0.7547211445483342 1104 22-23 0.583726765130919 1104 24-25 0.6102365482718994 1104 26-27 0.30110597308512155 1104 28-29 0.12922177140819713 1104 30-31 0.3338839497544086 1104 32-33 0.354547189588871 1104 34-35 0.44450932419515965 1104 36-37 0.6286171311967124 1104 38-39 0.3526712582299325 1104 40-41 0.7937285862163037 1104 42-43 0.8078240723622585 1104 44-45 0.8692644701406422 1104 46-47 0.967465736599948 1104 48-49 0.876757376372403 1104 50-51 0.7709158150332129 1104 52-53 0.6727789651833618 1104 54-55 0.8679598457808382 1104 56-57 0.9413165320052599 1104 58-59 1.0020129044083248 1104 60-61 1.046641872798638 1104 62-63 0.8240695646544722 1104 64-65 0.8356024834151157 1104 66-67 0.9177855625346112 1104 68-69 0.8245678457040526 1104 70-71 0.7868541644481724 1104 72-73 0.9922423666154003 1104 74-75 0.9494327364735433 1104 76-77 0.9706622451812699 1104 78-79 0.753390807645637 1104 80-81 0.8825958389308965 1104 82-83 0.8474335677570402 1104 84-85 0.9325758189861446 1104 86-87 0.6974993369032099 1104 88-89 0.7107811893890918 1104 90-91 0.8216478470160098 1104 92-93 0.7858444769975215 1104 94-95 0.9615694899829847 1104 96-97 0.8518730956857077 1104 98-99 0.7228351100374084 1104 100-101 1.2304669106695378 1105 1 0.39578224379256355 1105 2 0.39964812156085117 1105 3 0.46193235227608653 1105 4 0.5316358485984694 1105 5 0.5453739947508396 1105 6 0.49000534084413516 1105 7 0.5412468604321248 1105 8 0.47790168110929443 1105 9 0.4610873833724227 1105 10-11 0.6053047975789738 1105 12-13 0.5911169070990283 1105 14-15 0.7987590740615857 1105 16-17 0.7555750937198624 1105 18-19 0.7060256156516118 1105 20-21 0.6655047508489602 1105 22-23 0.608703831403794 1105 24-25 0.49806069692720456 1105 26-27 0.5166698630927371 1105 28-29 0.503205695424839 1105 30-31 0.3615146160630829 1105 32-33 0.5092382555037602 1105 34-35 0.35765762150576563 1105 36-37 0.38640452995269925 1105 38-39 0.35673779960013974 1105 40-41 0.394483161774545 1105 42-43 0.4282666932957184 1105 44-45 0.46017040829789124 1105 46-47 0.7229390663533906 1105 48-49 0.7931507960707975 1105 50-51 0.8504550149258208 1105 52-53 0.8875102490245865 1105 54-55 0.7305009586819082 1105 56-57 0.732817628349288 1105 58-59 0.8431585279819629 1105 60-61 0.622847571550146 1105 62-63 0.8467515801375285 1105 64-65 0.8545337157952986 1105 66-67 0.8393513767378593 1105 68-69 0.8579025774353823 1105 70-71 0.8735064555834384 1105 72-73 0.7301188153557234 1105 74-75 0.6688301947453432 1105 76-77 0.8393423252815033 1105 78-79 0.9357226083347712 1105 80-81 1.0761369182704037 1105 82-83 0.7881345007681837 1105 84-85 0.8339689030690209 1105 86-87 0.8924144821128586 1105 88-89 0.8848940457009 1105 90-91 0.8414734947539628 1105 92-93 0.7615674487610704 1105 94-95 0.9322764081375468 1105 96-97 1.0180486449764388 1105 98-99 1.1270448473623276 1105 100-101 1.7467403478199408 1106 1 0.2223870810356985 1106 2 0.1568238734174514 1106 3 0.3340635874392355 1106 4 0.3214075146999207 1106 5 0.3605547647188416 1106 6 0.3041810140623582 1106 7 0.4667379202316937 1106 8 0.5302189328946341 1106 9 0.6117099781439563 1106 10-11 0.6211303100800194 1106 12-13 0.581823448337488 1106 14-15 0.5063889625425446 1106 16-17 0.40259982917545045 1106 18-19 0.634384979516291 1106 20-21 0.41627439324093984 1106 22-23 0.192500086742605 1106 24-25 0.07517653002707902 1106 26-27 -0.008750145704929935 1106 28-29 0.1176867374324786 1106 30-31 0.10689184681265829 1106 32-33 0.33995117996668966 1106 34-35 0.2681788219205785 1106 36-37 0.05075689377314063 1106 38-39 0.01699359549422752 1106 40-41 0.05250175729313611 1106 42-43 0.10230862598127288 1106 44-45 0.18254314928766036 1106 46-47 0.3706326887810505 1106 48-49 0.44788915759107084 1106 50-51 0.4207365280264881 1106 52-53 0.4852552935781418 1106 54-55 0.6440071920019648 1106 56-57 0.6933896979639087 1106 58-59 0.6079995248708272 1106 60-61 0.6705388286828864 1106 62-63 0.7788270680640821 1106 64-65 0.44959138598594706 1106 66-67 0.4544563036778868 1106 68-69 0.4606010717562157 1106 70-71 0.4336102889585547 1106 72-73 0.4120935478623693 1106 74-75 0.5983425630875026 1106 76-77 0.4945328574667194 1106 78-79 0.6129152981604378 1106 80-81 0.7394906361454012 1106 82-83 0.5552913364090593 1106 84-85 0.9066041917947345 1106 86-87 1.210130264589278 1106 88-89 1.1608828004130594 1106 90-91 1.2614185523319996 1106 92-93 0.9764657042178371 1106 94-95 1.0853389428490168 1106 96-97 1.0036056012780747 1106 98-99 1.1714048784244895 1106 100-101 1.607601720649395 1107 1 0.17630573004960937 1107 2 0.18690616050522735 1107 3 0.2962574917234839 1107 4 0.2638094624120484 1107 5 0.25485317492984905 1107 6 0.14629272499829682 1107 7 0.36949324633027913 1107 8 0.3159246989383888 1107 9 0.44319163381562987 1107 10-11 0.4215905062023708 1107 12-13 0.2461503456484877 1107 14-15 0.15536246692131073 1107 16-17 0.158628973744662 1107 18-19 0.33096390927666164 1107 20-21 0.3220754438340663 1107 22-23 0.2403149713412418 1107 24-25 0.032041992238596606 1107 26-27 0.005310935576552822 1107 28-29 -0.013823912825259299 1107 30-31 0.13471711386507934 1107 32-33 0.22495338284409883 1107 34-35 0.10082397828931988 1107 36-37 0.0646503753570542 1107 38-39 0.1375333344007288 1107 40-41 0.16585879054899522 1107 42-43 -0.05539913380391681 1107 44-45 0.10280852247900896 1107 46-47 0.25516781053406845 1107 48-49 0.3682663716606527 1107 50-51 0.05647773728497185 1107 52-53 0.2221263542983678 1107 54-55 0.4719165659807274 1107 56-57 0.5053219883325681 1107 58-59 0.6496982326561493 1107 60-61 0.4914819058779827 1107 62-63 0.30606500888603705 1107 64-65 0.4371090065809611 1107 66-67 0.5403326432229179 1107 68-69 0.496060448466352 1107 70-71 0.4329062732568687 1107 72-73 0.47176097963701125 1107 74-75 0.5266460953521381 1107 76-77 0.5762457102139784 1107 78-79 0.6714567250237735 1107 80-81 0.6518033471198024 1107 82-83 0.5921744216398928 1107 84-85 0.4347714462724923 1107 86-87 0.7591606914497078 1107 88-89 0.7153231257083945 1107 90-91 0.7125886956014575 1107 92-93 0.8786893012855366 1107 94-95 0.6807405049363844 1107 96-97 0.9870549291176332 1107 98-99 1.1810635988937292 1107 100-101 1.5742624720326184 1108 1 0.012585662595984815 1108 2 0.05906692497965338 1108 3 0.20997081381454663 1108 4 0.2784008058634271 1108 5 0.313143225520065 1108 6 0.17809935793815157 1108 7 0.22911438179964705 1108 8 0.2041495443571577 1108 9 -0.2718100525250122 1108 10-11 -0.021569134044959526 1108 12-13 -0.03307112708900206 1108 14-15 0.31048556978808506 1108 16-17 0.3863293672241426 1108 18-19 0.2760662139422081 1108 20-21 0.09027611837031913 1108 22-23 0.2687337459337087 1108 24-25 0.17793462855113518 1108 26-27 0.14511644428931447 1108 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-4.912848633215145 2316 82-83 -4.285370862915752 2316 84-85 -3.905443635450659 2316 86-87 -4.256945582425775 2316 88-89 -5.648741630791921 2316 90-91 -5.063955236567626 2316 92-93 -4.465620541819778 2316 94-95 -3.982598729951878 2316 96-97 -5.622838906625397 2316 98-99 -7.3350968469222195 2316 100-101 -7.868793258785095 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3023.0 3 1111.0 4 1127.0 5 1197.0 6 1294.0 7 1584.0 8 1921.0 9 2535.0 10 3179.0 11 4047.0 12 5280.0 13 6481.0 14 7571.0 15 9328.0 16 11288.0 17 14189.0 18 11399.0 19 8735.0 20 8588.0 21 9320.0 22 10415.0 23 11885.0 24 13591.0 25 15613.0 26 18365.0 27 21924.0 28 26050.0 29 31101.0 30 37320.0 31 46574.0 32 57426.0 33 73530.0 34 97794.0 35 131676.0 36 184441.0 37 244064.0 38 203214.0 39 33233.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.195078716741605 18.748763950483582 14.078302961483418 21.977854371291393 2 15.30367372780599 20.924259907890573 39.647609224947445 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22.35325472112573 46-47 22.72969888764524 27.70186267468272 28.094559269076104 21.47387916859594 48-49 22.524936032941305 27.600810553233934 28.074155849932442 21.80009756389232 50-51 21.58988450865775 28.06311645422584 28.647773828452134 21.699225208664274 52-53 22.40570182314548 28.41698501043808 27.457323462306615 21.719989704109825 54-55 21.614104244004558 27.640462383068026 28.061486768794612 22.683946604132807 56-57 22.43593366203104 27.83589275407867 27.60090901699503 22.127264566895256 58-59 23.1613887164148 27.435582294204785 27.455452767884065 21.947576221496355 60-61 22.187653703411346 27.64563107681078 28.706884199189048 21.45983102058883 62-63 21.915510786834417 27.795075667532455 28.440696109214418 21.84871743641871 64-65 21.67466994891778 28.484781975424678 27.959886730252993 21.88066134540455 66-67 21.887710384434214 27.94268414152359 27.4878993858911 22.681706088151095 68-69 21.71553618162236 27.819892910243965 27.99606671562004 22.468504192513635 70-71 22.385227845304513 27.78226632667124 27.559609185126632 22.272896642897614 72-73 22.13259897372467 27.365531093108668 28.395999918318054 22.105870014848612 74-75 21.49958248706439 28.889707303376934 27.580138782029078 22.0305714275296 76-77 21.96616550538594 27.872196033084013 27.531050447609996 22.630588013920054 78-79 22.007303156095297 28.04400271881026 27.663602656966074 22.285091468128368 80-81 22.392037407876835 28.097584560791443 27.384008307675707 22.12636972365602 82-83 22.094121722010048 27.8464301014502 27.943304560245743 22.116143616294014 84-85 21.855114714614423 28.146033762304658 27.661485881208108 22.33736564187281 86-87 22.056840268498462 28.194817794458167 27.437018035160303 22.31132390188307 88-89 21.872490016577967 28.020557117424293 26.992190818350757 23.114762047646984 90-91 21.988660601972544 27.417497675605713 27.585583286237764 23.008258436183983 92-93 22.43735476547895 27.85604038873354 27.71113858759759 21.995466258189925 94-95 21.96727850969871 27.739063817808635 27.7024910673883 22.59116660510436 96-97 21.245700125650767 27.8836778702231 28.24334681727257 22.62727518685356 98-99 21.651341352456697 28.45078486395023 26.894002797029387 23.003870986563683 100-101 21.539274450192707 27.722343350184282 27.499499503879804 23.238882695743214 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 183.0 1 144.0 2 162.0 3 220.0 4 213.5 5 228.5 6 371.0 7 645.5 8 991.0 9 1246.5 10 1203.5 11 1128.5 12 1242.0 13 1364.0 14 1478.0 15 1602.5 16 1696.5 17 1818.5 18 1991.0 19 2128.5 20 2373.5 21 2899.0 22 3468.0 23 4164.0 24 5086.5 25 6422.0 26 8337.0 27 10530.5 28 12374.5 29 14712.5 30 17490.5 31 20371.0 32 24074.0 33 26748.5 34 29014.0 35 31183.5 36 33720.5 37 36757.5 38 38202.5 39 38902.0 40 40017.0 41 41856.0 42 43403.0 43 45220.5 44 47360.0 45 49491.5 46 50733.5 47 57680.5 48 76022.0 49 74317.5 50 55086.0 51 46350.5 52 42517.5 53 40793.5 54 39223.0 55 36272.5 56 32705.0 57 28732.5 58 25742.0 59 22861.0 60 19116.5 61 15725.0 62 12733.5 63 9796.0 64 7270.5 65 5676.0 66 4345.0 67 3304.0 68 2439.0 69 1708.0 70 1317.0 71 1067.0 72 851.5 73 709.5 74 595.5 75 485.0 76 341.0 77 227.0 78 157.5 79 110.0 80 71.5 81 49.0 82 38.5 83 31.0 84 22.0 85 12.0 86 8.0 87 7.5 88 8.0 89 7.5 90 3.5 91 1.0 92 0.0 93 1.5 94 3.5 95 2.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08246902489674966 2 0.03252094173647246 3 0.015750052500175 4 0.025593835312784374 5 0.02865634552115174 6 0.03660428868096227 7 0.08575028583428611 8 0.059791865972886576 9 0.07736484121613739 10-11 0.06588042793475979 12-13 0.04458869029563432 14-15 0.04965641552138507 16-17 0.06205229017430058 18-19 0.05888040460134867 20-21 0.019724024080080265 22-23 0.013526086753622513 24-25 0.023880287934293115 26-27 0.02468237394124647 28-29 0.006416688055626852 30-31 9.479198263994213E-4 32-33 0.009442739809132697 34-35 0.012031290104300346 36-37 0.004338556128520428 38-39 0.008239610798702661 40-41 0.004666682222274074 42-43 0.0025156333854446183 44-45 6.197937326457755E-4 46-47 6.562521875072917E-4 48-49 0.001093753645845486 50-51 0.0016406304687682292 52-53 2.1875072916909723E-4 54-55 5.104183680612269E-4 56-57 0.0038281377604592014 58-59 0.003098968663228877 60-61 0.005359392864642882 62-63 0.002187507291690972 64-65 5.46876822922743E-4 66-67 4.3750145833819446E-4 68-69 0.003718762395874653 70-71 0.0024062580208600694 72-73 0.018375061250204168 74-75 0.015239634132113773 76-77 0.0224948666495555 78-79 0.017208390694635648 80-81 0.011812539375131251 82-83 0.004484389947966493 84-85 0.003098968663228877 86-87 0.001421879739599132 88-89 0.0018593811979373265 90-91 0.007218774062580208 92-93 0.010973994913316378 94-95 0.01345316984389948 96-97 0.011885456284854282 98-99 0.023807371024570083 100-101 0.02074486081620272 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1371424.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.26270098939694 #Duplication Level Percentage of deduplicated Percentage of total 1 79.06011788449173 46.06256008490615 2 12.476045653633285 14.53776234895403 3 3.7970514303287564 6.636794163798189 4 1.6348683229632572 3.8100737703138035 5 0.8321550810116303 2.4241801330894006 6 0.4939330226070151 1.7266723202964938 7 0.32854369504986725 1.3399290146649419 8 0.21139212037007632 0.985302072050909 9 0.17345506776829425 0.9095364673631741 >10 0.8843062493975075 9.438995246439672 >50 0.06976172543229947 2.8231970645594937 >100 0.03335412828280772 3.7558193412638845 >500 0.0021316379320072062 0.8359783503650298 >1k 0.002633199798361843 3.529694485527083 >5k 1.2539046658865917E-4 0.41415374187008247 >10k+ 1.2539046658865917E-4 0.7693513945376325 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10531 0.7678879762932543 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5669 0.41336596121987074 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4879 0.3557616025386751 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4428 0.3228760762535875 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4156 0.3030426768089227 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3692 0.2692092306974357 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3314 0.2416466388221294 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2658 0.19381314604382016 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2654 0.193521478404928 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2560 0.18666728889096296 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2258 0.16464638215460717 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2195 0.16005261684205616 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2137 0.15582343607812027 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2026 0.14772965909886365 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1706 0.1243962479874933 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1549 0.1129482931609772 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 7.291690972303241E-5 0.0 0.0 0.0 0.0 7 7.291690972303241E-5 0.0 0.0 0.0 0.0 8 7.291690972303241E-5 0.0 0.0 0.0 0.0 9 7.291690972303241E-5 0.0 0.0 0.0 0.0 10-11 7.291690972303241E-5 0.0 0.0 0.0 0.0 12-13 7.291690972303241E-5 0.0 0.0 0.0 0.0 14-15 7.291690972303241E-5 0.0 0.0 0.0 0.0 16-17 7.291690972303241E-5 0.0 0.0 0.0 0.0 18-19 7.291690972303241E-5 0.0 0.0 0.0 0.0 20-21 7.291690972303241E-5 0.0 0.0 0.0 0.0 22-23 1.4583381944606482E-4 0.0 0.0 1.0937536458454862E-4 0.0 24-25 4.3750145833819446E-4 0.0 0.0 3.2812609375364583E-4 0.0 26-27 4.3750145833819446E-4 0.0 0.0 4.3750145833819446E-4 0.0 28-29 4.3750145833819446E-4 0.0 0.0 8.750029166763889E-4 0.0 30-31 4.3750145833819446E-4 0.0 0.0 0.0024427164757215855 0.0 32-33 5.104183680612269E-4 0.0 0.0 0.005250017500058334 0.0 34-35 5.833352777842593E-4 0.0 0.0 0.008093776979256598 0.0 36-37 5.833352777842593E-4 0.0 0.0 0.011958373194577316 0.0 38-39 5.833352777842593E-4 0.0 0.0 0.018958396527988426 0.0 40-41 6.197937326457754E-4 0.0 0.0 0.03190114800382668 0.0 42-43 8.020860069533565E-4 0.0 0.0 0.05217204890682969 0.0 44-45 8.750029166763889E-4 0.0 0.0 0.07240649135497118 0.0 46-47 0.0010208367361224538 0.0 0.0 0.11491704972349909 0.0 48-49 0.0010208367361224538 0.0 0.0 0.1572453158177194 0.0 50-51 0.0010208367361224538 0.0 0.0 0.18797979326597755 0.0 52-53 0.0010208367361224538 0.0 0.0 0.21809447698158993 0.0 54-55 0.0010208367361224538 0.0 0.0 0.24766228387427958 0.0 56-57 0.0010208367361224538 0.0 0.0 0.32211044870149563 0.0 58-59 0.0010208367361224538 0.0 0.0 0.41788680962269875 0.0 60-61 0.0010208367361224538 0.0 0.0 0.5092881559605199 0.0 62-63 0.0010208367361224538 0.0 0.0 0.6174603915346384 0.0 64-65 0.0010208367361224538 0.0 0.0 0.7558931446438155 0.0 66-67 0.001093753645845486 0.0 0.0 0.9513104627015424 0.0 68-69 0.001093753645845486 0.0 0.0 1.173269535898453 0.0 70-71 0.001093753645845486 0.0 0.0 1.3278169260564203 0.0 72-73 0.001093753645845486 0.0 0.0 1.4534892199640665 0.0 74-75 0.001093753645845486 0.0 0.0 1.6226199920666402 0.0 76-77 0.001093753645845486 0.0 0.0 1.833568611895373 0.0 78-79 0.001093753645845486 0.0 0.0 2.020017150057167 0.0 80-81 0.0012760459201530672 0.0 0.0 2.229142847142824 0.0 82-83 0.0013125043750145833 0.0 0.0 2.506956273187577 0.0 84-85 0.0013854212847376158 0.0 0.0 2.9760672118907063 0.0 86-87 0.001458338194460648 0.0 0.0 3.5559389364631215 0.0 88-89 0.001458338194460648 0.0 0.0 4.137925251417505 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 6090 0.0 25.129059 1 TACATGG 6145 0.0 24.440983 2 ACATGGG 5985 0.0 23.74523 3 ATTCGCG 110 2.1791493E-9 23.744284 80-81 GAGTACT 5005 0.0 22.889996 12-13 GTATAGG 385 0.0 22.220274 1 GTACTTT 5325 0.0 21.509342 14-15 TCGCGCT 100 5.409911E-7 21.367516 60-61 GTGTAGC 715 0.0 20.60598 1 GTATTAG 410 0.0 19.706192 1 CATGGGA 3490 0.0 19.47511 4 AGTACTT 5115 0.0 19.144955 12-13 CATGGGG 2560 0.0 19.123465 4 ACTTTTT 6275 0.0 18.40174 16-17 ATAGGAC 420 1.8189894E-12 18.10673 3 AATTCGC 125 4.5689976E-6 17.09838 78-79 TACTTTT 5980 0.0 16.490986 14-15 CAATTCG 130 6.635566E-6 16.440748 78-79 ATAGCCC 405 5.0931703E-10 16.43018 3 TAGCCCT 885 0.0 16.111921 4 >>END_MODULE