FastQCFastQC Report
Thu 26 May 2016
SRR936496_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936496_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1371424
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT107390.7830546935156452No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66090.4819078563595212No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60310.43976188253960846No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT58040.4232097440324801No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT47920.34941783139277127No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC41370.30165725552418504No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT36850.2686988123293744No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34800.2537508458361528No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC33740.24602165340551133No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT27640.20154233847446157No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC21560.1572088573628579No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA21290.155240100800336No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG20060.146271320904403No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC20000.1458338194460648No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA18940.13810462701542336No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA16560.12075040250134168No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16200.11812539375131251No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA15770.11498996663322211No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG58700.023.0668471
TATACCG1050.00219944122.6137285
GAGTACT72350.022.35042812-13
TACATGG58900.022.178672
ACATGGG58000.022.0257933
TACCGGT1100.002881577521.585834
AGTACTT73750.021.41099512-13
GTACTTT76300.021.06811114-15
GTCCTAC3054.5529305E-918.6923071
CATGGGA32650.018.6173654
TACTTTT85700.018.45248414-15
ACTTTTT89450.018.18325616-17
AGAGTAC123900.018.14913610-11
GGACGCT3156.828486E-918.0909826
CATGGGG24650.017.7240244
GTATAGC5700.017.5035421
GTGTAGG6150.016.9953081
GTATAGG3401.745866E-816.7680991
AGTACAT47950.016.7423422
TAGTACT3753.2905518E-916.4627934
Produced by FastQC (version 0.11.5)