##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936496_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1371424 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.884512740042467 31.0 31.0 34.0 30.0 34.0 2 32.03718397811326 31.0 31.0 34.0 30.0 34.0 3 32.11613184543948 33.0 31.0 34.0 30.0 34.0 4 35.82082492358308 37.0 35.0 37.0 35.0 37.0 5 35.64992810392701 37.0 35.0 37.0 33.0 37.0 6 35.74292268474228 37.0 35.0 37.0 33.0 37.0 7 35.685984786615954 37.0 35.0 37.0 33.0 37.0 8 35.662630229600765 37.0 35.0 37.0 33.0 37.0 9 37.28702866509555 39.0 37.0 39.0 34.0 39.0 10-11 37.18051200795669 39.0 37.0 39.0 33.0 39.0 12-13 37.07574717957393 39.0 37.0 39.0 33.0 39.0 14-15 38.181826335254456 40.0 37.0 41.0 33.0 41.0 16-17 38.10443159810533 40.0 37.0 41.0 33.0 41.0 18-19 38.269263553795184 40.0 38.0 41.0 33.5 41.0 20-21 38.2888198689829 40.0 38.0 41.0 34.0 41.0 22-23 38.217097702825676 40.0 38.0 41.0 33.0 41.0 24-25 38.068237102457005 40.0 37.0 41.0 33.0 41.0 26-27 37.866959087780295 40.0 37.0 41.0 33.0 41.0 28-29 37.78217713850713 40.0 37.0 41.0 33.0 41.0 30-31 37.74125762710875 40.0 37.0 41.0 33.0 41.0 32-33 37.559866970389905 40.0 36.5 41.0 32.5 41.0 34-35 37.3619682898943 39.0 36.0 41.0 32.0 41.0 36-37 37.135634202114005 39.0 36.0 41.0 31.0 41.0 38-39 36.95936085411951 39.0 36.0 41.0 31.0 41.0 40-41 36.77420221609072 39.0 35.0 41.0 31.0 41.0 42-43 36.47992378724595 39.0 35.0 41.0 30.0 41.0 44-45 36.16719628648762 39.0 35.0 40.0 30.0 41.0 46-47 35.75323933371445 39.0 35.0 40.0 28.5 41.0 48-49 35.32428519553399 38.0 35.0 40.0 27.0 41.0 50-51 35.01617661642206 38.0 34.0 40.0 26.0 41.0 52-53 34.67433995613319 38.0 34.0 40.0 24.0 41.0 54-55 34.5063849691999 38.0 34.0 40.0 24.5 41.0 56-57 34.203045885152946 38.0 33.5 40.0 23.5 41.0 58-59 33.84337301957673 37.5 33.0 40.0 23.0 41.0 60-61 33.410100377417926 37.0 32.5 40.0 22.0 41.0 62-63 33.11360563910213 36.5 32.0 39.0 22.0 40.5 64-65 32.702616769139226 36.0 31.5 39.0 20.0 40.0 66-67 32.28292344307815 35.5 31.0 39.0 19.0 40.0 68-69 31.92096390321301 35.0 31.0 38.0 18.0 40.0 70-71 31.5459405698019 35.0 31.0 38.0 17.0 40.0 72-73 31.093235206617354 35.0 30.0 37.0 13.5 39.0 74-75 30.607945099400332 34.0 30.0 36.5 9.5 39.0 76-77 29.0839302797676 32.5 28.0 35.0 8.0 37.5 78-79 29.853694772732574 34.0 29.0 36.0 7.0 37.5 80-81 29.599450279417596 34.0 29.0 35.0 4.5 37.0 82-83 29.291533836362788 34.0 29.0 35.0 2.0 37.0 84-85 28.923008857946193 34.0 29.0 35.0 2.0 36.0 86-87 28.563971827822762 34.0 29.0 35.0 2.0 36.0 88-89 28.100565543551813 33.0 27.5 35.0 2.0 35.5 90-91 27.608428538511795 33.0 26.5 35.0 2.0 35.0 92-93 26.987138186293954 33.0 25.5 34.5 2.0 35.0 94-95 26.46967495099984 32.0 25.0 34.0 2.0 35.0 96-97 25.79249451664839 32.0 24.0 34.0 2.0 35.0 98-99 24.966462596541987 32.0 19.0 34.0 2.0 35.0 100-101 21.871430717269057 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.44386422477643706 1101 2 0.3726822346561285 1101 3 0.4500435238264089 1101 4 0.3830768632650958 1101 5 0.3820194613008283 1101 6 0.39919635670458575 1101 7 0.40241509308476964 1101 8 0.3338520320952867 1101 9 0.26953515262216143 1101 10-11 0.3917245135212539 1101 12-13 0.4626203346622262 1101 14-15 0.6680293668539079 1101 16-17 0.6237868444231864 1101 18-19 0.5628368621172299 1101 20-21 0.5589004304439271 1101 22-23 0.47455425752755787 1101 24-25 0.42386232932987866 1101 26-27 0.4149822784362769 1101 28-29 0.459979385118892 1101 30-31 0.4861528290520951 1101 32-33 0.5426951285863808 1101 34-35 0.664863274891097 1101 36-37 0.701667853185775 1101 38-39 0.6227541484635992 1101 40-41 0.6705812191326217 1101 42-43 0.579637552646254 1101 44-45 0.5818801840883054 1101 46-47 0.5561380544209484 1101 48-49 0.5377625053460093 1101 50-51 0.5183962961643402 1101 52-53 0.562901118721328 1101 54-55 0.5795971525195611 1101 56-57 0.5482698765222977 1101 58-59 0.359465383148482 1101 60-61 0.49430281470402093 1101 62-63 0.5066309927588932 1101 64-65 0.5900258955612117 1101 66-67 0.4870735636702861 1101 68-69 0.3825091813234387 1101 70-71 0.34214632439038795 1101 72-73 0.47276103568944094 1101 74-75 0.4636244505199265 1101 76-77 0.2701093160562209 1101 78-79 0.49983026723906576 1101 80-81 0.4829076737469542 1101 82-83 0.38084503064036923 1101 84-85 0.5958068164456236 1101 86-87 0.5452640354957587 1101 88-89 0.396184299683096 1101 90-91 0.6322256251770426 1101 92-93 0.7327202439924676 1101 94-95 0.5512985893714664 1101 96-97 0.7854654406498334 1101 98-99 0.800981318788299 1101 100-101 1.432419844314552 1102 1 0.35664281417219357 1102 2 0.33281497996308573 1102 3 0.37137586491100194 1102 4 0.26402129367404115 1102 5 0.25523835649958926 1102 6 0.2429707281278226 1102 7 0.28328290797728783 1102 8 0.3274855298270296 1102 9 0.36678797833421584 1102 10-11 0.37783980477005485 1102 12-13 0.38996836682426306 1102 14-15 0.5075924936457241 1102 16-17 0.39177927074596397 1102 18-19 0.38203893485528795 1102 20-21 0.3438570149846214 1102 22-23 0.28288598898330264 1102 24-25 0.3860510616314201 1102 26-27 0.44028352295785567 1102 28-29 0.3683945972807052 1102 30-31 0.4963347291169029 1102 32-33 0.592255893752963 1102 34-35 0.6226008928350595 1102 36-37 0.6188978315466969 1102 38-39 0.6280969136521435 1102 40-41 0.6264044102924444 1102 42-43 0.8921415344562433 1102 44-45 0.8607770030340234 1102 46-47 0.934610192735299 1102 48-49 1.0776130089763143 1102 50-51 1.0226612265787836 1102 52-53 1.0984277886100529 1102 54-55 0.9267373891214561 1102 56-57 0.9845173913236493 1102 58-59 1.070756697859764 1102 60-61 1.1208690390693974 1102 62-63 1.150073912826116 1102 64-65 1.1213199014268298 1102 66-67 1.009576144981601 1102 68-69 1.1723959331550695 1102 70-71 1.1788395273034276 1102 72-73 1.1733853560356629 1102 74-75 1.0683953454111261 1102 76-77 0.8187600316835919 1102 78-79 0.9936400630408677 1102 80-81 1.1146182593201672 1102 82-83 1.086340614950391 1102 84-85 1.0754167912915698 1102 86-87 1.211321439465486 1102 88-89 1.241292577454164 1102 90-91 1.2810641723930694 1102 92-93 1.490505369048762 1102 94-95 1.31889585537834 1102 96-97 1.3474097722000486 1102 98-99 1.2610316818195315 1102 100-101 2.0536298477196837 1103 1 0.33031608770669507 1103 2 0.3442161909676038 1103 3 0.31429988005928067 1103 4 0.2238036953557483 1103 5 0.3105597383659884 1103 6 0.1889398694716391 1103 7 0.19700724618019194 1103 8 0.23032703837237278 1103 9 0.424667626449434 1103 10-11 0.3079952688458576 1103 12-13 0.3509917580872184 1103 14-15 0.39368832452996116 1103 16-17 0.343503584049607 1103 18-19 0.2413297881130987 1103 20-21 0.08095224643248855 1103 22-23 0.13679635112710997 1103 24-25 0.1799172620285887 1103 26-27 0.2191786475508266 1103 28-29 0.2110340721924544 1103 30-31 0.22487481413991617 1103 32-33 0.27752491036208937 1103 34-35 0.18728148171838654 1103 36-37 0.2348971142361762 1103 38-39 0.29516444070293346 1103 40-41 0.28346985269265446 1103 42-43 0.4445038779849355 1103 44-45 0.5177888060766662 1103 46-47 0.4196122310308894 1103 48-49 0.4872745300613559 1103 50-51 0.6311128556656698 1103 52-53 0.5008787231665934 1103 54-55 0.39156400950022885 1103 56-57 0.4100144427483059 1103 58-59 0.5609516881821648 1103 60-61 0.3858032295375935 1103 62-63 0.5192157334333274 1103 64-65 0.6904601062311713 1103 66-67 0.3894529890166183 1103 68-69 0.6355740372779444 1103 70-71 0.5101142129966583 1103 72-73 0.49425692010370526 1103 74-75 0.4347202388674667 1103 76-77 0.3772746108609759 1103 78-79 0.3016779141501935 1103 80-81 0.2620208032296141 1103 82-83 0.5634227027690137 1103 84-85 1.018995603930751 1103 86-87 0.8307011936674336 1103 88-89 0.8656401308239978 1103 90-91 0.8357687678688741 1103 92-93 0.9907631028508028 1103 94-95 0.9734915033589999 1103 96-97 1.1056345399151404 1103 98-99 1.0186152936581188 1103 100-101 1.3809366778243728 1104 1 0.11329165041975031 1104 2 0.23464892731514553 1104 3 0.17179076678062444 1104 4 0.21578266698571724 1104 5 0.1635639639186266 1104 6 0.09346225845350631 1104 7 0.17233544509546306 1104 8 0.18537258461840622 1104 9 0.18102005849272018 1104 10-11 0.23008365354575488 1104 12-13 0.22225746287877968 1104 14-15 0.26197639815612206 1104 16-17 0.31924050377485713 1104 18-19 0.22146028865170564 1104 20-21 0.3106256727297705 1104 22-23 0.3374747766319359 1104 24-25 0.20317056147870005 1104 26-27 0.173320253260826 1104 28-29 0.28236854630859654 1104 30-31 0.18726121715502586 1104 32-33 0.06191507957223763 1104 34-35 -0.013991019416572215 1104 36-37 0.1326748711204928 1104 38-39 0.20806661365240586 1104 40-41 0.25001894888642795 1104 42-43 0.2100096145823116 1104 44-45 0.24727647460851898 1104 46-47 0.29358653386842803 1104 48-49 0.611333854349013 1104 50-51 0.5943431603728797 1104 52-53 0.5163084374246765 1104 54-55 0.44715312890365055 1104 56-57 0.5026268443289226 1104 58-59 0.49091241445069755 1104 60-61 0.5834069140690659 1104 62-63 0.44744084014666186 1104 64-65 0.44202823487278664 1104 66-67 0.5237882233561564 1104 68-69 0.3843583514248117 1104 70-71 0.5052283673891615 1104 72-73 0.6296801066485607 1104 74-75 0.5205936495841996 1104 76-77 0.29737204302514186 1104 78-79 0.26343169310516856 1104 80-81 0.6377088322631614 1104 82-83 0.4968122245804416 1104 84-85 0.6450089441051965 1104 86-87 0.8052952263167477 1104 88-89 0.6338736725248033 1104 90-91 0.7273896554766957 1104 92-93 0.7337617946218558 1104 94-95 0.8073432033850558 1104 96-97 1.0186112577379483 1104 98-99 0.9867811405550384 1104 100-101 1.5250579750932083 1105 1 0.06738895654585164 1105 2 0.0900103852289611 1105 3 0.049468419261941676 1105 4 0.1534873924522202 1105 5 0.23447672625809446 1105 6 0.13786475095808726 1105 7 0.12644673520151883 1105 8 0.13386567093871804 1105 9 0.10928783492803262 1105 10-11 0.13167950533794937 1105 12-13 0.29589570291098966 1105 14-15 0.404024547795899 1105 16-17 0.13460358383034787 1105 18-19 0.20641296686354593 1105 20-21 0.3614224799074677 1105 22-23 0.3279298712755079 1105 24-25 0.2766787504973678 1105 26-27 0.15336539354811407 1105 28-29 0.11357435693194162 1105 30-31 0.14870738442579778 1105 32-33 0.10958115331815321 1105 34-35 0.1935116655075788 1105 36-37 0.3009950035767517 1105 38-39 0.30191058599768894 1105 40-41 0.3733624066212471 1105 42-43 0.1418511369342994 1105 44-45 0.2280294839610022 1105 46-47 0.2919604766795416 1105 48-49 0.3535892465716799 1105 50-51 0.3931564798340972 1105 52-53 0.4826649394159972 1105 54-55 0.3317827771785886 1105 56-57 0.33511862174870544 1105 58-59 0.2902255213554312 1105 60-61 0.5886313289793392 1105 62-63 0.4331164901220461 1105 64-65 0.44272184027843764 1105 66-67 0.36896458931131093 1105 68-69 0.3727289940166578 1105 70-71 0.3623761389021176 1105 72-73 0.6560636816250671 1105 74-75 0.6113365345352015 1105 76-77 0.29659481341937877 1105 78-79 0.26263600464586645 1105 80-81 0.40166903944980703 1105 82-83 0.5921880020897241 1105 84-85 0.7367290295752831 1105 86-87 0.7552306203293639 1105 88-89 0.7894376184683303 1105 90-91 0.739185981605484 1105 92-93 0.42793187337962735 1105 94-95 0.8348569636213306 1105 96-97 0.8520525473965144 1105 98-99 0.4214225353731358 1105 100-101 0.8082654526069675 1106 1 0.18132828458092476 1106 2 0.11852754805463306 1106 3 0.08470424838075274 1106 4 0.18658462634732587 1106 5 0.23163130718926794 1106 6 0.17198244066088364 1106 7 0.0854656968800569 1106 8 0.1529434639526812 1106 9 0.26424475584239815 1106 10-11 0.172029286379221 1106 12-13 0.22920314087269134 1106 14-15 0.379206767658971 1106 16-17 0.3532362406279006 1106 18-19 0.23913789719485834 1106 20-21 0.12958041894306405 1106 22-23 0.09615431503187466 1106 24-25 0.20948625112401942 1106 26-27 0.08024967349090417 1106 28-29 -0.07138202948720362 1106 30-31 0.034427699667396894 1106 32-33 0.180827676027441 1106 34-35 0.24506741520162478 1106 36-37 -0.008395401558331628 1106 38-39 0.17502556882011788 1106 40-41 0.41752630251387046 1106 42-43 0.41117866822991544 1106 44-45 0.4796590800054972 1106 46-47 0.44097129191376183 1106 48-49 0.423300170670899 1106 50-51 0.23333304142023792 1106 52-53 0.061211929532134945 1106 54-55 0.19305316172024334 1106 56-57 -0.02689544837706137 1106 58-59 0.07496279545440387 1106 60-61 -0.05589126235175712 1106 62-63 0.07874140103951532 1106 64-65 0.06097184150715407 1106 66-67 0.11954193629824061 1106 68-69 -0.03755982385194656 1106 70-71 0.17306208057210526 1106 72-73 0.31657213402181483 1106 74-75 0.206860505724066 1106 76-77 0.14208315159388718 1106 78-79 0.008427162006174882 1106 80-81 0.15329295264286813 1106 82-83 0.11940886042760468 1106 84-85 0.391357420240908 1106 86-87 0.5301404012231892 1106 88-89 0.2674105791540562 1106 90-91 0.4619337907894625 1106 92-93 0.6565270295013832 1106 94-95 0.4084219361224726 1106 96-97 0.7793412313341328 1106 98-99 0.7575799386847955 1106 100-101 1.13977320831685 1107 1 0.021300149812020663 1107 2 -0.007962222545504716 1107 3 -0.04627014749264191 1107 4 -0.010257905251762622 1107 5 0.008480956522859628 1107 6 -0.026920096478527 1107 7 0.004938325118068576 1107 8 -4.524420893474712E-4 1107 9 -0.0431602416347161 1107 10-11 0.039263173777435156 1107 12-13 -0.0075145426893854506 1107 14-15 0.040891989890994296 1107 16-17 0.030345483630007664 1107 18-19 -0.13912201389486967 1107 20-21 -0.09297921147778254 1107 22-23 -0.08229410935319237 1107 24-25 -0.057397761804274694 1107 26-27 -0.0014685127086409011 1107 28-29 -0.057550933133057924 1107 30-31 -0.07627586026655564 1107 32-33 -0.11067232025530416 1107 34-35 -0.04771341772846682 1107 36-37 -0.02458404544243109 1107 38-39 -0.09534829972029968 1107 40-41 -0.10350220688965095 1107 42-43 0.014000032164979359 1107 44-45 0.19670876935614245 1107 46-47 0.10612519756855221 1107 48-49 -0.006188196099941479 1107 50-51 -0.12577476575186353 1107 52-53 0.07249816104727813 1107 54-55 0.0168329890521548 1107 56-57 -0.21655123383135333 1107 58-59 -0.09790667622026916 1107 60-61 -0.11317464095478869 1107 62-63 -0.05239332164197208 1107 64-65 -0.24513938974131122 1107 66-67 -0.019186975958717767 1107 68-69 0.0896719430949382 1107 70-71 -0.011360456851761569 1107 72-73 0.1493629097999296 1107 74-75 0.17576744861830562 1107 76-77 0.049737334426570357 1107 78-79 0.009435830967195358 1107 80-81 0.12101538427043224 1107 82-83 0.02063833431324369 1107 84-85 0.2146548125706289 1107 86-87 0.012612414336370392 1107 88-89 -0.009261773295985876 1107 90-91 0.22621863463729497 1107 92-93 0.343073897132534 1107 94-95 0.3252902234475066 1107 96-97 0.5870925799702746 1107 98-99 0.6018217981395999 1107 100-101 0.9238041087231963 1108 1 -0.039256342599447436 1108 2 0.05456166728024314 1108 3 -0.12671534142181784 1108 4 0.10134636681119247 1108 5 0.10768612796749011 1108 6 0.07279210138543846 1108 7 -0.0893224955677141 1108 8 0.028660542733589978 1108 9 0.005407493047691503 1108 10-11 0.11425642841486905 1108 12-13 0.17185083111612443 1108 14-15 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-1.2560317144592261 2316 54-55 -0.9078877705276724 2316 56-57 -0.997994858686198 2316 58-59 -1.4620275166118901 2316 60-61 -0.9879138189943788 2316 62-63 -1.0404557379144599 2316 64-65 -0.8927337094628314 2316 66-67 -1.0173387568134586 2316 68-69 -1.5608336404388332 2316 70-71 -1.0530059327299526 2316 72-73 -0.6868759514931391 2316 74-75 -0.8800092559177308 2316 76-77 -0.8674063639746556 2316 78-79 -1.0251041982729063 2316 80-81 -0.8967549241527273 2316 82-83 -1.1419571516982856 2316 84-85 -0.8113024693490729 2316 86-87 -0.9776481080850772 2316 88-89 -1.0517268387675536 2316 90-91 -1.0761557533791084 2316 92-93 -1.0169540938277635 2316 94-95 -1.175274439367218 2316 96-97 -1.1777202591620046 2316 98-99 -1.7112977535971545 2316 100-101 -2.240745391046733 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 20.0 5 40.0 6 82.0 7 271.0 8 572.0 9 1105.0 10 1901.0 11 3014.0 12 4331.0 13 6069.0 14 7670.0 15 10565.0 16 13334.0 17 16816.0 18 14801.0 19 9600.0 20 8451.0 21 8595.0 22 9285.0 23 10184.0 24 11968.0 25 14604.0 26 17743.0 27 21769.0 28 27081.0 29 33392.0 30 41053.0 31 50738.0 32 63011.0 33 79655.0 34 104732.0 35 140806.0 36 199424.0 37 260885.0 38 167264.0 39 10592.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.48437122809423 19.283422686096486 15.161502293318707 22.070703792490576 2 15.513456669117046 21.284976254652804 39.094351291116794 24.107215785113357 3 20.470411670374915 24.466132416225285 30.560131615505952 24.503324297893844 4 12.49927083090277 16.9768065893553 38.10171033903446 32.42221224070747 5 12.970175232631142 38.10432871486105 34.43393027591975 14.49156577658806 6 30.913561378537928 37.01940464801549 16.93480644935483 15.132227524091746 7 27.41568429288411 33.73408496138682 20.74356343739313 18.10666730833594 8 26.199410248034162 33.54141388804629 19.867087056956855 20.39208880696269 9 25.635252117507058 17.37959959866533 21.197018573395248 35.78812971043237 10-11 24.96678634762116 26.364676423921413 27.248793954313182 21.41974327414425 12-13 26.793927974542875 24.025013781496916 27.692131567357247 21.488926676602958 14-15 22.80536027959321 25.42439557218389 27.63837654520384 24.131867603019057 16-17 21.319344462159208 28.83762747247659 27.96616070819845 21.876867357165747 18-19 20.969305554998545 27.610562747898232 30.604318419547262 20.815813277555968 20-21 22.06906835522785 25.713747170823904 30.63035210117367 21.586832372774577 22-23 21.430959459976776 25.854468626553818 30.81358953932869 21.900982374140717 24-25 22.154745724152413 25.973331369437897 30.76615984553282 21.10576306087687 26-27 21.748481389294092 25.77738904325247 31.3638639012718 21.11026566618164 28-29 20.879459433642037 27.196834713151347 30.950435566017063 20.97327028718955 30-31 21.251086024833736 26.244266353993602 30.64378348994331 21.860864131229352 32-33 21.284108493778835 26.35340111399081 30.72940291084427 21.633087481386085 34-35 22.366831717642153 25.93506099083816 30.432891483814466 21.265215807705225 36-37 22.030542244052114 25.602444779541084 31.108120681458235 21.258892294948563 38-39 21.547326117130286 26.422328350242395 30.930334558476364 21.10001097415095 40-41 21.585509909436933 26.573442125679954 30.623623689314726 21.21742427556839 42-43 21.35097009094386 26.83888501798058 29.737362821268675 22.07278206980688 44-45 21.747704706090815 26.32936000110836 29.62826848321378 22.294666809587042 46-47 22.40568230415946 26.85641222517902 29.211861758890723 21.526043711770797 48-49 22.367210716841733 27.00626325504781 28.938578975019535 21.687947053090923 50-51 21.424425578395578 27.309407819469268 29.55430235601999 21.711864246115166 52-53 22.2654012643086 27.78204950119274 28.095045906747124 21.857503327751534 54-55 21.506868044225282 27.035538824730427 28.96796128009042 22.48963185095387 56-57 22.34899397383435 27.11482824735065 28.447833884072704 22.088343894742295 58-59 22.971703944832374 26.77561949247331 28.239163280809237 22.013513281885075 60-61 22.053513276575945 27.04436368198066 29.581890147976186 21.32023289346721 62-63 21.818209663945066 27.194818805132332 29.15490528479105 21.83206624613155 64-65 21.52919060547327 27.724283294466012 28.831555282461203 21.914970817599514 66-67 21.743006274008803 27.2185810998037 28.356857220360336 22.681555405827154 68-69 21.74224529015242 27.122006316863285 28.69535226010339 22.440396132880903 70-71 22.33244034294331 27.073176600637876 28.390787080854764 22.20359597556405 72-73 22.014089005296682 26.673078493594975 29.15965449051497 22.15317801059337 74-75 21.546511729653577 28.082953428477257 28.185513155937453 22.18502168593171 76-77 21.870508633268216 27.15230122044858 28.197306219059477 22.779883927223725 78-79 21.750616125253096 27.307867187497152 28.43948116766082 22.502035519588937 80-81 22.33992394078481 27.40086284070849 27.927129306135605 22.33208391237109 82-83 21.85951903507995 27.13133856912444 28.630310774359575 22.37883162143604 84-85 21.590039187511056 27.4448247503141 28.311472330772496 22.653663731402343 86-87 21.800794238543578 27.42467550604357 28.022560429330746 22.751969826082107 88-89 21.56054154487208 27.29408477705664 27.536676979236503 23.608696698834784 90-91 21.616074219683288 26.75482576401167 28.149654767146103 23.47944524915894 92-93 21.91138289037908 27.02952773063946 28.357460490435386 22.701628888546065 94-95 21.3963235371886 26.976810851046835 28.388043599961925 23.23882201180264 96-97 20.77067837162212 27.06376990176198 29.03462205301446 23.130929673601436 98-99 21.083569963141198 27.400236094776442 27.645742560388026 23.870451381694338 100-101 20.472158854811752 26.738144862460594 28.305963841114547 24.483732441613107 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 344.0 1 276.5 2 210.0 3 252.5 4 253.5 5 247.0 6 386.0 7 740.5 8 1145.5 9 1409.0 10 1403.5 11 1303.0 12 1442.5 13 1642.0 14 1789.5 15 1918.0 16 2099.5 17 2260.5 18 2448.5 19 2760.5 20 3108.5 21 3498.5 22 4062.5 23 4794.5 24 5727.5 25 7081.5 26 8885.5 27 11128.0 28 13082.0 29 15391.0 30 18251.5 31 20851.0 32 24245.5 33 26363.0 34 28266.0 35 30876.0 36 33699.5 37 36695.0 38 37973.0 39 38523.5 40 39836.5 41 41953.0 42 43551.0 43 44759.0 44 46257.0 45 48295.0 46 49141.0 47 57076.5 48 77219.0 49 74151.0 50 53408.0 51 45393.5 52 41812.0 53 40045.0 54 38179.5 55 35314.5 56 32051.5 57 28491.0 58 25906.5 59 23131.0 60 19427.5 61 15911.0 62 12817.0 63 9740.5 64 6979.5 65 5364.0 66 4331.5 67 3428.5 68 2491.5 69 1727.0 70 1340.0 71 1062.0 72 873.5 73 776.0 74 654.0 75 527.5 76 400.5 77 270.5 78 172.5 79 130.0 80 104.0 81 81.0 82 61.0 83 45.5 84 27.0 85 15.0 86 10.5 87 7.5 88 5.0 89 3.0 90 1.5 91 0.5 92 0.5 93 1.0 94 0.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019468814896049656 2 0.015166717222390741 3 0.011447954826516088 4 0.0 5 0.0034270947569825234 6 0.0 7 7.291690972303241E-5 8 0.0 9 0.0 10-11 0.0 12-13 0.001458338194460648 14-15 2.1875072916909723E-4 16-17 3.6458454861516205E-5 18-19 0.001421879739599132 20-21 0.0 22-23 1.0937536458454862E-4 24-25 0.0 26-27 0.0016406304687682292 28-29 0.003463553211844039 30-31 0.0017135473784912617 32-33 0.010062533541778473 34-35 0.002151048836829456 36-37 8.750029166763889E-4 38-39 0.001421879739599132 40-41 2.9166763889212964E-4 42-43 0.006088561961873206 44-45 0.002260424201414005 46-47 6.197937326457755E-4 48-49 9.114613715379052E-4 50-51 7.291690972303241E-5 52-53 1.82292274307581E-4 54-55 0.002661467204890683 56-57 0.01728130760435868 58-59 0.029349056163520546 60-61 0.02369799565998553 62-63 0.0170990153300511 64-65 0.022349032830109434 66-67 0.0037916793055976852 68-69 0.0011302121007070024 70-71 1.4583381944606482E-4 72-73 0.0 74-75 0.0020416734722449076 76-77 0.0031354271180903935 78-79 0.011119828732762443 80-81 0.01863027043423478 82-83 0.02074486081620272 84-85 0.03292198473994914 86-87 0.012651083836946124 88-89 0.04411473038243461 90-91 0.04976579088596963 92-93 0.03343240310801036 94-95 0.033505320017733396 96-97 0.05268246727489092 98-99 0.05745852486174954 100-101 0.06485959119863734 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1371424.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.2174386449754 #Duplication Level Percentage of deduplicated Percentage of total 1 77.61176132202358 42.85522668927312 2 12.967485511599117 14.320626712326634 3 4.152724331176289 6.879084028986697 4 1.8386599628010925 4.061043747397683 5 0.9426814673457429 2.602622804245947 6 0.5722903879885505 1.8960245631520174 7 0.36174227421914734 1.3982137282392746 8 0.2515379069957145 1.1111423157137117 9 0.18251617664319023 0.9070268206959765 >10 0.9934990794199471 10.13743101670509 >50 0.07520618954929367 2.846844806733662 >100 0.043312925984855574 4.363365085322115 >500 0.003422881411745097 1.2693715083465695 >1k 0.0026329857013423826 3.2305657543482935 >5k 3.949478552013574E-4 1.3407563910173022 >10k+ 1.3164928506711912E-4 0.7806540274959233 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10739 0.7830546935156452 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6609 0.4819078563595212 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6031 0.43976188253960846 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5804 0.4232097440324801 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4792 0.34941783139277127 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4137 0.30165725552418504 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3685 0.2686988123293744 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3480 0.2537508458361528 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3374 0.24602165340551133 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2764 0.20154233847446157 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2156 0.1572088573628579 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2129 0.155240100800336 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2006 0.146271320904403 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2000 0.1458338194460648 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1894 0.13810462701542336 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1656 0.12075040250134168 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1620 0.11812539375131251 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1577 0.11498996663322211 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 7.291690972303241E-5 0.0 0.0 0.0 0.0 12-13 7.291690972303241E-5 0.0 0.0 0.0 0.0 14-15 7.291690972303241E-5 0.0 0.0 7.291690972303241E-5 0.0 16-17 7.291690972303241E-5 0.0 0.0 7.291690972303241E-5 0.0 18-19 7.291690972303241E-5 0.0 0.0 7.291690972303241E-5 0.0 20-21 7.291690972303241E-5 0.0 0.0 7.291690972303241E-5 0.0 22-23 7.291690972303241E-5 0.0 0.0 1.82292274307581E-4 0.0 24-25 7.291690972303241E-5 0.0 0.0 4.0104300347667827E-4 0.0 26-27 7.291690972303241E-5 0.0 0.0 5.104183680612269E-4 0.0 28-29 1.0937536458454862E-4 0.0 0.0 0.0011302121007070024 0.0 30-31 1.4583381944606482E-4 0.0 0.0 0.002916676388921296 0.0 32-33 1.4583381944606482E-4 0.0 0.0 0.005651060503535012 0.0 34-35 1.4583381944606482E-4 0.0 0.0 0.008604195347317824 0.0 36-37 2.1875072916909723E-4 0.0 0.0 0.012760459201530672 0.0 38-39 2.1875072916909723E-4 0.0 0.0 0.020234442448141494 0.0 40-41 2.1875072916909723E-4 0.0 0.0 0.033359486198287325 0.0 42-43 2.9166763889212964E-4 0.0 0.0 0.05373976246587489 0.0 44-45 3.64584548615162E-4 0.0 0.0 0.07415649718832396 0.0 46-47 4.0104300347667827E-4 0.0 0.0 0.11684934783115944 0.0 48-49 4.3750145833819446E-4 0.0 0.0 0.1595057400191334 0.0 50-51 4.3750145833819446E-4 0.0 0.0 0.18972979909933033 0.0 52-53 4.3750145833819446E-4 0.0 0.0 0.21977156590521968 0.0 54-55 4.3750145833819446E-4 0.0 0.0 0.24963104043680145 0.0 56-57 4.3750145833819446E-4 0.0 0.0 0.3234594115313718 0.0 58-59 4.3750145833819446E-4 0.0 0.0 0.41927223090743637 0.0 60-61 4.3750145833819446E-4 0.0 0.0 0.5111110787035957 0.0 62-63 4.3750145833819446E-4 0.0 0.0 0.6199031080103601 0.0 64-65 4.3750145833819446E-4 0.0 0.0 0.7570598151993839 0.0 66-67 5.104183680612269E-4 0.0 0.0 0.9524771332571109 0.0 68-69 5.104183680612269E-4 0.0 0.0 1.174545581818606 0.0 70-71 5.833352777842593E-4 0.0 0.0 1.3293846396154654 0.0 72-73 6.562521875072917E-4 0.0 0.0 1.4551298504328347 0.0 74-75 6.562521875072917E-4 0.0 0.0 1.622948118160394 0.0 76-77 6.562521875072917E-4 0.0 0.0 1.8341154887182958 0.0 78-79 6.927106423688079E-4 0.0 0.0 2.0203088176960593 0.0 80-81 8.020860069533565E-4 0.0 0.0 2.2278303427678092 0.0 82-83 8.750029166763889E-4 0.0 0.0 2.503966679888933 0.0 84-85 9.479198263994213E-4 0.0 0.0 2.9701609422031403 0.0 86-87 0.0010208367361224538 0.0 0.0 3.5427045173483913 0.0 88-89 0.0010208367361224538 0.0 0.0 4.117544975149917 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 5870 0.0 23.066847 1 TATACCG 105 0.002199441 22.613728 5 GAGTACT 7235 0.0 22.350428 12-13 TACATGG 5890 0.0 22.17867 2 ACATGGG 5800 0.0 22.025793 3 TACCGGT 110 0.0028815775 21.58583 4 AGTACTT 7375 0.0 21.410995 12-13 GTACTTT 7630 0.0 21.068111 14-15 GTCCTAC 305 4.5529305E-9 18.692307 1 CATGGGA 3265 0.0 18.617365 4 TACTTTT 8570 0.0 18.452484 14-15 ACTTTTT 8945 0.0 18.183256 16-17 AGAGTAC 12390 0.0 18.149136 10-11 GGACGCT 315 6.828486E-9 18.090982 6 CATGGGG 2465 0.0 17.724024 4 GTATAGC 570 0.0 17.503542 1 GTGTAGG 615 0.0 16.995308 1 GTATAGG 340 1.745866E-8 16.768099 1 AGTACAT 4795 0.0 16.742342 2 TAGTACT 375 3.2905518E-9 16.462793 4 >>END_MODULE