FastQCFastQC Report
Thu 26 May 2016
SRR936495_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936495_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1047989
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT76310.7281564978258359No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT44230.4220464146093137No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40260.3841643376027802No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35040.33435465448587726No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC27810.2653653807435002No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT27780.2650791181968513No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT23320.22252141959505303No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18480.1763377287357024No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT17610.16803611488288522No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC17570.1676544314873534No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC16950.16173833885661015No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG14110.1346388177738507No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA13320.12710057071209718No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC13000.1240471035478426No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA12210.11650885648608908No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA12200.11641343563720613No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA11420.10897060942433558No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGATT352.7816402E-454.3101438
TATCACG608.284605E-539.587912
TACGCTA600.003939336631.6854579
TACCTGG8750.026.0601542
GAGTACT38450.024.22300312-13
GTACATG48500.023.898531
GGCCGAT1201.7054641E-423.7606897
GTACCTG10250.023.6357481
GTACACG1452.3328637E-522.932591
TACATGG49800.022.798822
GAGTACG1903.2842217E-722.5015281
ACATGGG48050.022.2471523
GTACTTT42550.021.89260314-15
GTGTAGC4400.021.5923751
ATGGGCG1553.896767E-521.458195
AGTACTT40100.020.08600612-13
GTGCGGA1200.004761352719.7930091
ACTTTTT49900.019.3341916-17
GGGACGC1507.699577E-419.0076436
CATGGGA24800.018.583444
Produced by FastQC (version 0.11.5)