##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936495_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1047989 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.54586164549437 31.0 31.0 34.0 30.0 34.0 2 31.60536036160685 33.0 31.0 34.0 30.0 34.0 3 31.624260369145095 33.0 31.0 34.0 28.0 34.0 4 35.24782321188486 37.0 35.0 37.0 33.0 37.0 5 35.26895129624452 37.0 35.0 37.0 33.0 37.0 6 35.263373947627315 37.0 35.0 37.0 33.0 37.0 7 35.18867373607929 37.0 35.0 37.0 32.0 37.0 8 35.15629171680237 37.0 35.0 37.0 32.0 37.0 9 36.67355000863559 39.0 37.0 39.0 32.0 39.0 10-11 36.54794182000002 39.0 37.0 39.0 32.0 39.0 12-13 36.461295395276096 39.0 37.0 39.0 32.0 39.0 14-15 37.56753410579691 40.0 37.0 41.0 32.0 41.0 16-17 37.49004951387849 40.0 37.0 41.0 32.0 41.0 18-19 37.48510814521909 40.0 37.0 41.0 32.0 41.0 20-21 37.35271028608125 40.0 37.0 41.0 32.0 41.0 22-23 37.46210360986613 40.0 37.0 41.0 32.0 41.0 24-25 37.50085258528477 40.0 37.0 41.0 32.0 41.0 26-27 37.40593508137967 40.0 37.0 41.0 32.0 41.0 28-29 37.27954730440872 40.0 37.0 41.0 31.0 41.0 30-31 37.07899605816473 39.0 36.0 41.0 31.0 41.0 32-33 36.8825865538665 39.0 36.0 41.0 30.5 41.0 34-35 36.68183253831863 39.0 35.5 41.0 30.0 41.0 36-37 36.477026476422935 39.0 35.0 41.0 30.0 41.0 38-39 36.27251908178425 39.0 35.0 40.0 30.0 41.0 40-41 36.09695998717544 39.0 35.0 40.0 29.5 41.0 42-43 35.86469228207548 38.5 35.0 40.0 29.0 41.0 44-45 35.57606902362525 38.0 35.0 40.0 27.5 41.0 46-47 35.36639506712379 38.0 35.0 40.0 26.5 41.0 48-49 35.173878733459986 38.5 35.0 40.0 26.0 41.0 50-51 34.18094750994524 37.5 33.5 39.5 24.0 40.5 52-53 34.285150416655135 38.0 33.5 39.5 24.0 40.5 54-55 34.72867606434801 38.0 34.0 40.0 24.5 41.0 56-57 34.61075831902816 38.0 34.0 40.0 24.5 41.0 58-59 34.380507810673585 38.0 34.0 40.0 23.5 41.0 60-61 34.05572339022642 38.0 33.5 40.0 23.0 41.0 62-63 33.72234632233735 37.0 33.0 40.0 22.0 41.0 64-65 33.37931934400075 36.5 33.0 39.5 22.0 41.0 66-67 32.8870885095168 36.0 32.0 39.0 20.0 41.0 68-69 32.49811209850485 35.5 32.0 39.0 18.5 40.5 70-71 32.08063157151459 35.0 31.5 38.5 18.0 40.0 72-73 31.67750472571754 35.0 31.0 37.5 15.5 40.0 74-75 31.250998340631437 35.0 31.0 37.0 12.0 39.0 76-77 30.787761131080572 35.0 30.5 36.5 7.5 39.0 78-79 30.282827873193327 34.0 30.0 36.0 7.0 38.5 80-81 29.803722176473226 34.0 29.0 35.5 2.0 37.0 82-83 29.384830852232227 34.0 29.0 35.0 2.0 37.0 84-85 29.030182568710167 34.0 29.0 35.0 2.0 36.5 86-87 28.70235708580911 34.0 29.0 35.0 2.0 36.0 88-89 28.292449157386194 34.0 28.5 35.0 2.0 36.0 90-91 27.854486545183207 34.0 27.0 35.0 2.0 35.0 92-93 27.41261120107177 33.5 26.5 35.0 2.0 35.0 94-95 26.931025993593444 33.0 25.5 35.0 2.0 35.0 96-97 26.34966158995944 33.0 25.0 35.0 2.0 35.0 98-99 25.63488452645972 33.0 22.0 35.0 2.0 35.0 100-101 22.742010173770907 29.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4425671605770347 1101 2 0.45701765914888526 1101 3 0.39677311609048616 1101 4 0.3819386128890798 1101 5 0.4013476550537973 1101 6 0.5008920283315561 1101 7 0.5168242536858827 1101 8 0.4953376498105797 1101 9 0.7325944776656144 1101 10-11 0.7586061389777043 1101 12-13 0.7817841514913937 1101 14-15 0.8341781340326335 1101 16-17 0.7650816442698982 1101 18-19 0.8850428395609384 1101 20-21 0.8218786273123797 1101 22-23 0.7695811415196729 1101 24-25 0.7230925214473558 1101 26-27 0.6600715969880326 1101 28-29 0.8117094602421275 1101 30-31 0.8600437012473634 1101 32-33 0.7157153386871045 1101 34-35 0.6142206093840414 1101 36-37 0.5401270643977725 1101 38-39 0.5179757514733154 1101 40-41 0.5259653184195088 1101 42-43 0.7306535899179849 1101 44-45 0.5578126136694692 1101 46-47 0.6871401372832935 1101 48-49 0.7014333800736736 1101 50-51 0.5568040276838175 1101 52-53 0.5646615634178858 1101 54-55 0.6031535524492355 1101 56-57 0.5790748346167689 1101 58-59 0.5531486155615184 1101 60-61 0.6926381610720398 1101 62-63 0.8042006932472319 1101 64-65 0.8707550508718214 1101 66-67 1.0366713148937023 1101 68-69 0.8104987053279515 1101 70-71 0.7047739720080841 1101 72-73 0.6794640763274238 1101 74-75 0.6882412527257529 1101 76-77 0.5208206133967437 1101 78-79 0.6370244433791363 1101 80-81 0.5490347610781932 1101 82-83 0.4711618527375343 1101 84-85 0.4143421172207482 1101 86-87 0.7572844807739472 1101 88-89 0.7967565552109441 1101 90-91 0.7437652632213165 1101 92-93 0.9357582440683316 1101 94-95 0.8093147792064705 1101 96-97 0.7196259349340295 1101 98-99 0.8219616236455884 1101 100-101 1.2103663860418017 1102 1 0.4231217168085877 1102 2 0.4438739303974266 1102 3 0.39027360129732 1102 4 0.4567069282854703 1102 5 0.5095288661990836 1102 6 0.43356775484748056 1102 7 0.40366021395069396 1102 8 0.6074410048399415 1102 9 0.7068386900308923 1102 10-11 0.6661227959756388 1102 12-13 0.5730426625237115 1102 14-15 0.6671116640152306 1102 16-17 0.6397675209676308 1102 18-19 0.715622353079695 1102 20-21 0.5928679713472533 1102 22-23 0.449298832120995 1102 24-25 0.5620367087225944 1102 26-27 0.3525868667325014 1102 28-29 0.23183618485040824 1102 30-31 0.6224778730835965 1102 32-33 0.6719956143323742 1102 34-35 0.4668889814653312 1102 36-37 0.24257214840958596 1102 38-39 0.3670448977615166 1102 40-41 0.3287934569643056 1102 42-43 0.4931833428541168 1102 44-45 0.5552063693031144 1102 46-47 0.7631725791016137 1102 48-49 0.8181800516609954 1102 50-51 0.897467347241971 1102 52-53 1.1303208008665635 1102 54-55 1.169189140478764 1102 56-57 1.0327750843223882 1102 58-59 1.1277689130045943 1102 60-61 1.0886957062042484 1102 62-63 1.3291546616550178 1102 64-65 1.1866711625264728 1102 66-67 1.3295649928676951 1102 68-69 1.3219010659156112 1102 70-71 1.2976522508048731 1102 72-73 1.1613464797141724 1102 74-75 1.3668182758258496 1102 76-77 1.3547673694140379 1102 78-79 1.0977754585964412 1102 80-81 1.4714401656583895 1102 82-83 1.4259961323680095 1102 84-85 1.073901820229029 1102 86-87 0.8801533209371257 1102 88-89 0.9468411179340457 1102 90-91 1.0057759576843814 1102 92-93 1.046739184931507 1102 94-95 0.9835531505045267 1102 96-97 1.015865947040968 1102 98-99 0.9312304016810522 1102 100-101 0.7057127812404431 1103 1 0.3353819590567113 1103 2 0.3320997071712988 1103 3 0.3416057121807192 1103 4 0.4775670423667009 1103 5 0.5692730399984711 1103 6 0.633532223430727 1103 7 0.6025213051458849 1103 8 0.6064118627333102 1103 9 0.336854488821551 1103 10-11 0.63812721202315 1103 12-13 0.275143774783956 1103 14-15 0.7063726105467865 1103 16-17 0.739204411461067 1103 18-19 0.7231721446498582 1103 20-21 0.8584104282178231 1103 22-23 0.36306789060190425 1103 24-25 0.4411521667923495 1103 26-27 0.36664905940160253 1103 28-29 0.5658105641609694 1103 30-31 0.5534565098192772 1103 32-33 0.5318032370902444 1103 34-35 0.7054437410889562 1103 36-37 0.7500169319534606 1103 38-39 0.6789384050295979 1103 40-41 0.61336235301588 1103 42-43 0.6070685129290538 1103 44-45 0.738867628003554 1103 46-47 0.8747589934106301 1103 48-49 0.7515916387097903 1103 50-51 0.7904359336626214 1103 52-53 0.9524146799455337 1103 54-55 0.876315093006248 1103 56-57 1.047078700278533 1103 58-59 0.9827144420737355 1103 60-61 0.895931108185124 1103 62-63 0.9113223318727179 1103 64-65 1.0453804838129699 1103 66-67 1.2801807650098027 1103 68-69 1.2190265108514282 1103 70-71 1.2599699143604255 1103 72-73 0.9703914459910585 1103 74-75 1.018946671138238 1103 76-77 1.0833159110397261 1103 78-79 1.159359017702318 1103 80-81 1.137908660078054 1103 82-83 0.8222164779110379 1103 84-85 1.04879126041887 1103 86-87 0.9503405588136182 1103 88-89 0.8305791498742536 1103 90-91 0.9967455846679556 1103 92-93 0.9148259450373395 1103 94-95 0.9401362971921756 1103 96-97 1.276172913102119 1103 98-99 1.4786032975549688 1103 100-101 1.47153853771065 1104 1 0.431184372244271 1104 2 0.480737332504102 1104 3 0.39108172192410606 1104 4 0.4739076077096982 1104 5 0.5386380204550534 1104 6 0.5602925474549707 1104 7 0.5983633716501586 1104 8 0.5028827175455248 1104 9 0.6553102753734095 1104 10-11 0.7428629509910323 1104 12-13 0.7029010964856468 1104 14-15 0.5161528178769927 1104 16-17 0.45290180000692004 1104 18-19 0.47998968274536935 1104 20-21 0.2840264708168192 1104 22-23 0.42055953508158694 1104 24-25 0.528497094000187 1104 26-27 0.23610184363091236 1104 28-29 0.34727830335544496 1104 30-31 0.5032044280201475 1104 32-33 0.4985001158077438 1104 34-35 0.6952868272499444 1104 36-37 0.3081458618803836 1104 38-39 0.4991984174920674 1104 40-41 0.36372206524609396 1104 42-43 0.461181128348521 1104 44-45 0.4559808665465752 1104 46-47 0.36921319382981466 1104 48-49 0.6326175266608445 1104 50-51 0.5775825942868806 1104 52-53 0.6306761652454895 1104 54-55 0.454092241366304 1104 56-57 0.7227570552323712 1104 58-59 0.7110693959059446 1104 60-61 0.6870374357215212 1104 62-63 0.627631174562346 1104 64-65 0.5182406209001513 1104 66-67 0.7459882764809365 1104 68-69 0.5645343565433834 1104 70-71 0.5131166882798794 1104 72-73 0.40551861984252113 1104 74-75 0.36094519830076166 1104 76-77 0.5036693920344568 1104 78-79 0.6717138582393396 1104 80-81 0.818062175061339 1104 82-83 0.6134502625368796 1104 84-85 0.450776758634472 1104 86-87 0.4971597963369838 1104 88-89 0.4321114673273776 1104 90-91 0.46157309274023817 1104 92-93 0.1793503884747203 1104 94-95 0.09216711348402384 1104 96-97 0.4269149966006758 1104 98-99 0.32643935941413815 1104 100-101 0.14779186575687575 1105 1 0.21359141364412082 1105 2 0.02424011269753379 1105 3 0.16690062723941423 1105 4 0.07199177773631504 1105 5 0.17888626142271846 1105 6 0.3306854638910508 1105 7 0.3977651688936845 1105 8 0.33430549141434085 1105 9 0.33396734971393727 1105 10-11 0.3391367012216122 1105 12-13 0.2371422749714327 1105 14-15 0.23609774105932502 1105 16-17 0.32900135991943813 1105 18-19 0.34009983638070196 1105 20-21 0.21044094363416121 1105 22-23 0.03441570289459861 1105 24-25 0.06416130988023383 1105 26-27 -0.2239569225429605 1105 28-29 -0.25311303192798107 1105 30-31 -0.43989384061769243 1105 32-33 -0.3818883206957935 1105 34-35 -0.20222549256453703 1105 36-37 -0.11525519749189783 1105 38-39 -0.24009190848890682 1105 40-41 -0.14103325137972433 1105 42-43 -0.10298599948766451 1105 44-45 0.031143593507863443 1105 46-47 -0.013014650268530659 1105 48-49 0.017476672311367736 1105 50-51 0.02662467930019119 1105 52-53 0.1780369579300185 1105 54-55 0.19902859997172584 1105 56-57 0.036186311827087536 1105 58-59 -0.00601100570683144 1105 60-61 0.14712518969416521 1105 62-63 0.2545371223315982 1105 64-65 0.31992981899222883 1105 66-67 0.3951067838728264 1105 68-69 0.3449227930469263 1105 70-71 0.20946949614347687 1105 72-73 0.06894787643724953 1105 74-75 0.37520983055100743 1105 76-77 0.19242871424701846 1105 78-79 0.2481652246187629 1105 80-81 0.3543114174256914 1105 82-83 0.1552440802299806 1105 84-85 -0.09382360503063936 1105 86-87 -0.06786774207185076 1105 88-89 0.12850248638026684 1105 90-91 0.14711476928910727 1105 92-93 -0.2042952225090211 1105 94-95 0.028082996162495988 1105 96-97 0.014164318022658051 1105 98-99 -0.0041546269352394916 1105 100-101 -0.19949777505794586 1106 1 0.25482560524327624 1106 2 0.34840524197215217 1106 3 0.43355927273017514 1106 4 0.3579279826506223 1106 5 0.32560961106577224 1106 6 0.3987371202638741 1106 7 0.48124422025594527 1106 8 0.4714328409504489 1106 9 0.554648614920751 1106 10-11 0.551498670152931 1106 12-13 0.6768364007969438 1106 14-15 0.7462111070121082 1106 16-17 0.5080848068860959 1106 18-19 0.4560044913364507 1106 20-21 0.4119422403909354 1106 22-23 0.4649616776011598 1106 24-25 0.47728930111041734 1106 26-27 0.32859514825728553 1106 28-29 0.3904700273485133 1106 30-31 0.6449100416314479 1106 32-33 0.5694605737187928 1106 34-35 0.4531821168576755 1106 36-37 0.420515236453717 1106 38-39 0.3508735737174504 1106 40-41 0.365917097541427 1106 42-43 0.681348643971063 1106 44-45 0.7055266313945552 1106 46-47 0.48454691225654045 1106 48-49 0.7196731376465166 1106 50-51 0.6053592051150005 1106 52-53 0.5708352788305291 1106 54-55 0.6784502731890214 1106 56-57 0.6246291342115242 1106 58-59 0.6933673531605749 1106 60-61 0.7079978936325588 1106 62-63 0.873529930010541 1106 64-65 0.6673242736859493 1106 66-67 0.9782783568254203 1106 68-69 1.024960759946211 1106 70-71 0.7999176125040037 1106 72-73 0.6154198959019688 1106 74-75 0.8180816788973075 1106 76-77 0.8806950707615506 1106 78-79 0.6595860422439586 1106 80-81 0.7591455127707363 1106 82-83 0.700631904084954 1106 84-85 0.7828909213189199 1106 86-87 0.7694882109775314 1106 88-89 0.5841253306892433 1106 90-91 0.683917261201092 1106 92-93 0.8226051277489894 1106 94-95 0.9394810871226298 1106 96-97 0.8387224849165911 1106 98-99 1.0197177294188684 1106 100-101 0.9637478600855012 1107 1 0.24255624726011504 1107 2 0.17346298285063355 1107 3 0.14102417810715195 1107 4 0.23006935906970938 1107 5 0.2053264878629406 1107 6 0.34061661800825505 1107 7 0.39886174673444685 1107 8 0.4223507794596273 1107 9 0.37174042934748286 1107 10-11 0.47628095231935674 1107 12-13 0.32333979837825666 1107 14-15 0.19919665764661687 1107 16-17 0.20286935239207793 1107 18-19 0.18853202434777216 1107 20-21 0.27324093434113195 1107 22-23 0.3978816942675607 1107 24-25 0.3487573340111396 1107 26-27 0.3268256282395896 1107 28-29 0.10612230637713083 1107 30-31 0.37269124871158255 1107 32-33 0.05416399907745273 1107 34-35 0.06444891829592336 1107 36-37 0.25850050417077597 1107 38-39 0.18226557898485396 1107 40-41 0.2914102740870561 1107 42-43 0.25438918346890915 1107 44-45 0.46769851145813135 1107 46-47 0.39820513769544874 1107 48-49 0.2966866096608314 1107 50-51 0.11412012958633255 1107 52-53 0.23395236035705125 1107 54-55 0.4652182243008909 1107 56-57 0.24647524769742546 1107 58-59 0.22612584968583604 1107 60-61 0.18903676648945833 1107 62-63 0.42375317759416475 1107 64-65 0.3938187207880972 1107 66-67 0.3754496967738774 1107 68-69 0.4280389017444364 1107 70-71 0.22410679682728585 1107 72-73 0.3430845924344297 1107 74-75 0.37389331760658706 1107 76-77 0.1868536691370899 1107 78-79 0.14991463799607985 1107 80-81 0.24291273858515083 1107 82-83 0.32505256631753454 1107 84-85 0.20002285858326374 1107 86-87 1.5106083067450982E-4 1107 88-89 -0.18221238014855246 1107 90-91 -0.03750138974565331 1107 92-93 0.3330674148536126 1107 94-95 0.39781249147338826 1107 96-97 0.25508231475460974 1107 98-99 0.16837207117285402 1107 100-101 0.34037707877039836 1108 1 0.38167212250695215 1108 2 0.25953182284957776 1108 3 0.14645329059095857 1108 4 0.26942972154297706 1108 5 0.2758965137402285 1108 6 0.16710267796189981 1108 7 0.3003947553221522 1108 8 0.15859591235280135 1108 9 0.34875851757773546 1108 10-11 0.3136266433093269 1108 12-13 0.1185203010421958 1108 14-15 0.2821001557768241 1108 16-17 0.3380892924710537 1108 18-19 0.3553575767781041 1108 20-21 0.5130971328825353 1108 22-23 0.2995434987486476 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-1.80495596669099 2316 70-71 -1.6683100013543317 2316 72-73 -1.604291560182471 2316 74-75 -1.4379976192761603 2316 76-77 -1.4723548589872593 2316 78-79 -2.162436849363594 2316 80-81 -2.233822514370214 2316 82-83 -2.1211682398443124 2316 84-85 -1.7646537138889187 2316 86-87 -1.6267028037238127 2316 88-89 -1.8097114123970108 2316 90-91 -2.0863733388468866 2316 92-93 -2.594632115662815 2316 94-95 -2.984051348366215 2316 96-97 -3.271921427218018 2316 98-99 -4.263128985909599 2316 100-101 -5.0063439804957 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2021.0 3 819.0 4 836.0 5 912.0 6 1001.0 7 1232.0 8 1540.0 9 1939.0 10 2487.0 11 3244.0 12 4154.0 13 5188.0 14 5694.0 15 7014.0 16 8688.0 17 11139.0 18 9123.0 19 6649.0 20 6746.0 21 7288.0 22 7944.0 23 8982.0 24 10316.0 25 11795.0 26 13867.0 27 16115.0 28 19291.0 29 22869.0 30 28347.0 31 34756.0 32 43611.0 33 55857.0 34 74075.0 35 102147.0 36 144188.0 37 191711.0 38 152572.0 39 21827.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.23990395443562 18.553094186494835 14.273798571532186 21.93320328753736 2 15.48244253682558 20.798305092833385 39.66903501949239 24.050217350848648 3 20.510796763547727 24.754393432422166 29.636557633320415 25.09825217070969 4 12.649303623562558 16.615781351255656 37.95554605399411 32.77936897118769 5 13.491203671444648 37.59756331533437 33.90807834273371 15.00315467048727 6 30.835274574199957 36.200355151607724 17.75261590162682 15.211754372565492 7 28.41072943475701 33.04981959517941 20.707667849007702 17.83178312105588 8 26.23662297698178 33.242212405936094 19.857171764220784 20.663992852861337 9 26.097016649262038 17.935995509796548 21.078947016744717 34.8880408241967 10-11 25.097847171309873 26.38265212609971 27.15593116141251 21.363569541177906 12-13 27.003149485291345 23.990258024887567 27.3192434553562 21.687349034464887 14-15 23.16473293947691 25.656012435159965 26.853903574058545 24.32535105130458 16-17 21.825974075558864 28.99137124639673 27.20792050856194 21.974734169482467 18-19 21.53481258068134 28.039810349641 29.222476478630927 21.202900591046728 20-21 22.837230662297685 26.49687220732797 28.86047524117229 21.80542188920205 22-23 22.079387688394853 26.44358711912448 29.209678073547064 22.267347118933607 24-25 22.780293791521384 26.547253028281776 29.142118833460245 21.530334346736595 26-27 22.328729215868698 26.254777070063696 29.919797705097928 21.49669600896968 28-29 21.480235002466628 27.63612022645276 29.437045617845225 21.446599153235386 30-31 21.83234872290095 26.628233302575193 29.35061514693772 22.18880282758614 32-33 21.867476490149958 26.889590778317977 29.33494916337855 21.907983568153515 34-35 22.939135305396956 26.611265295372995 28.846881430473715 21.602717968756334 36-37 22.65142153752201 26.18019341879893 29.566787692571932 21.601597351107124 38-39 22.122131052902272 26.82952779084513 29.44262773750488 21.605713418747715 40-41 22.10703148557404 27.03259247384472 29.183794986888962 21.676581053692274 42-43 21.877542178998983 27.22195688798405 28.372924329485006 22.527576603531962 44-45 22.261923619367877 26.80163975411322 28.377269404806665 22.559167221712237 46-47 22.882005690002227 27.30723960462929 28.001385524607613 21.809369180760864 48-49 22.745131717371233 27.24086518620301 27.920838372778174 22.09316472364758 50-51 21.950309486219 27.591246308852597 28.530845801765643 21.927598403162758 52-53 22.586918549033136 28.09038030935651 27.370736853254858 21.951964288355498 54-55 21.91303949523057 27.291482970139057 27.903645684229012 22.891831850401363 56-57 22.640248248775503 27.545203586706247 27.4757843892208 22.33876377529745 58-59 23.356014804586426 27.169336164978482 27.324405476189227 22.15024355424587 60-61 22.498722517425527 27.29241922039926 28.63903614595793 21.569822116217278 62-63 22.20210423059894 27.528187453243564 28.24904769538466 22.020660620772837 64-65 21.928382357712252 28.316135200795433 27.639978549249893 22.115503892242426 66-67 22.09440323346464 27.77090883929786 27.271525612179893 22.863162315057608 68-69 21.808211044031786 27.637764005513997 27.836138063762274 22.717886886691947 70-71 22.58076528298284 27.651111164156305 27.355882844389278 22.412240708471582 72-73 22.36479444785897 27.18216626646765 28.163878634716916 22.289160650956468 74-75 21.777518770472597 28.64261239731784 27.356087475103557 22.223781357106002 76-77 22.204680743226312 27.743609543222114 27.23534147739448 22.816368236157093 78-79 22.25085172369014 27.757885128064103 27.553691057283192 22.43757209096256 80-81 22.59654300470627 27.878023796391144 27.25061663837174 22.274816560530848 82-83 22.370521503846692 27.79312274082151 27.698984336362436 22.137371418969366 84-85 22.090769879025352 28.05260447908271 27.412012905362428 22.444612736529514 86-87 22.210970721867522 28.099142791560865 27.25787309421085 22.432013392360762 88-89 22.053435890436717 27.92406333050662 26.85137507677228 23.17112570228439 90-91 22.212044477645424 27.428024544328476 27.418716601789512 22.941214376236584 92-93 22.623693908581473 27.731660314578686 27.444122147168 22.20052362967184 94-95 22.164505221729037 27.572094026433604 27.515835061503118 22.74756569033424 96-97 21.540642726627635 27.6202183588868 28.08988227860968 22.74925663587589 98-99 21.87793870967434 28.16001092185622 26.978464658152586 22.98358571031686 100-101 21.651856053444256 27.68434009216766 27.330164950698133 23.333638903689955 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 115.0 1 101.0 2 169.0 3 246.5 4 212.0 5 196.0 6 301.0 7 567.5 8 913.5 9 1178.0 10 1165.5 11 1052.5 12 1105.5 13 1216.5 14 1359.0 15 1428.5 16 1468.0 17 1597.5 18 1708.5 19 1797.0 20 1985.5 21 2285.5 22 2630.5 23 3116.5 24 3683.5 25 4490.5 26 5653.0 27 7067.0 28 8420.5 29 9924.5 30 12092.5 31 14600.5 32 17142.5 33 19108.5 34 21195.0 35 23121.0 36 24768.5 37 26704.5 38 27669.5 39 28162.0 40 29169.0 41 31315.5 42 33356.0 43 34714.5 44 36224.0 45 37922.5 46 38947.5 47 43922.5 48 56668.5 49 55562.5 50 42229.5 51 36010.5 52 33410.0 53 32427.0 54 31300.5 55 28988.0 56 26135.0 57 23325.0 58 21228.5 59 18938.5 60 15886.5 61 13006.0 62 10669.0 63 8383.5 64 6323.0 65 4901.5 66 3714.5 67 2946.5 68 2250.5 69 1481.0 70 1122.5 71 913.5 72 717.0 73 606.5 74 494.0 75 370.5 76 253.5 77 169.5 78 106.0 79 68.0 80 41.5 81 26.0 82 18.5 83 12.5 84 12.5 85 8.5 86 6.5 87 4.5 88 3.5 89 2.5 90 2.0 91 1.5 92 1.5 93 2.0 94 1.5 95 1.0 96 1.0 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014599389879092244 2 0.013740602239145642 3 0.016698648554517272 4 0.019847536567654814 5 0.03206140522467316 6 0.05238604603674276 7 0.006488617724040998 8 0.028339992118237886 9 0.03578281833110844 10-11 0.0416989109618517 12-13 0.03411295347565671 14-15 0.030009856973689613 16-17 0.03139345928249247 18-19 0.025906760471722513 20-21 0.057729613574188286 22-23 0.01493336285018259 24-25 0.0033397297109034546 26-27 0.0013358918843613817 28-29 0.0 30-31 0.0026717837687227635 32-33 0.00229010037319094 34-35 4.771042444147791E-5 36-37 3.816833955318233E-4 38-39 0.0 40-41 0.0016221544310102494 42-43 0.004246227775291535 44-45 0.0025763629198398076 46-47 0.0010019189132710363 48-49 6.20235517739213E-4 50-51 0.004055386077525623 52-53 0.003769123530876756 54-55 0.00620235517739213 56-57 7.633667910636466E-4 58-59 0.006727169846248387 60-61 0.002814915042047197 62-63 4.771042444147792E-4 64-65 6.679459421806909E-4 66-67 0.018750196805500823 68-69 0.04513406152163811 70-71 0.06335944365828268 72-73 0.016889490252283184 74-75 0.038120629128740854 76-77 0.029914436124806657 78-79 0.06689001506695204 80-81 0.09365556317862116 82-83 0.05677540508535873 84-85 0.019799826143213337 86-87 0.021708243120872453 88-89 0.02361666009853157 90-91 0.04737645147038757 92-93 0.028817096362652665 94-95 0.014885652425741112 96-97 0.02084945548092585 98-99 0.052290625187859804 100-101 0.07318779109322712 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1047989.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.83827648434295 #Duplication Level Percentage of deduplicated Percentage of total 1 85.00609114274489 53.41636258081059 2 9.029575889249655 11.348059725300534 3 2.359596403607087 4.448189136039702 4 1.059246054350336 2.6624478571286305 5 0.5762870110596259 1.8106441267650184 6 0.37216815284901567 1.4031843172436194 7 0.2502635255009177 1.1008290026561152 8 0.1847380825379871 0.9286898166167521 9 0.14581847985079113 0.8246683758051545 >10 0.9094957483526468 10.751885220223285 >50 0.07217131249447518 3.094358116667391 >100 0.029982357187995766 3.3408706390763925 >500 0.00197853075968395 0.8375845539723101 >1k 0.0024351147811494766 3.302424324414003 >5k 1.521946738218423E-4 0.729802207280509 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7631 0.7281564978258359 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4423 0.4220464146093137 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4026 0.3841643376027802 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3504 0.33435465448587726 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2781 0.2653653807435002 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2778 0.2650791181968513 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2332 0.22252141959505303 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1848 0.1763377287357024 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1761 0.16803611488288522 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1757 0.1676544314873534 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1695 0.16173833885661015 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1411 0.1346388177738507 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1332 0.12710057071209718 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1300 0.1240471035478426 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1221 0.11650885648608908 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1220 0.11641343563720613 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1142 0.10897060942433558 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 9.542084888295584E-5 0.0 18-19 4.771042444147792E-5 0.0 0.0 1.4313127332443376E-4 0.0 20-21 9.542084888295584E-5 0.0 0.0 2.385521222073896E-4 0.0 22-23 9.542084888295584E-5 0.0 0.0 3.8168339553182334E-4 0.0 24-25 9.542084888295584E-5 0.0 0.0 4.7710424441477916E-4 0.0 26-27 9.542084888295584E-5 0.0 0.0 7.156563666221688E-4 0.0 28-29 9.542084888295584E-5 0.0 0.0 0.0013358918843613817 0.0 30-31 9.542084888295584E-5 0.0 0.0 0.002433231646515374 0.0 32-33 9.542084888295584E-5 0.0 0.0 0.004007675653084145 0.0 34-35 9.542084888295584E-5 0.0 0.0 0.005868382206301784 0.0 36-37 9.542084888295584E-5 0.0 0.0 0.0076336679106364675 0.0 38-39 9.542084888295584E-5 0.0 0.0 0.010925687197098443 0.0 40-41 9.542084888295584E-5 0.0 0.0 0.013931443936911552 0.0 42-43 9.542084888295584E-5 0.0 0.0 0.021421980574223586 0.0 44-45 9.542084888295584E-5 0.0 0.0 0.02929420060706744 0.0 46-47 2.385521222073896E-4 0.0 0.0 0.03859773337315564 0.0 48-49 3.8168339553182334E-4 0.0 0.0 0.05057304990796659 0.0 50-51 3.8168339553182334E-4 0.0 0.0 0.06712856718915944 0.0 52-53 3.8168339553182334E-4 0.0 0.0 0.09189027747428646 0.0 54-55 3.8168339553182334E-4 0.0 0.0 0.10858892602880374 0.0 56-57 3.8168339553182334E-4 0.0 0.0 0.1508126516595117 0.0 58-59 3.8168339553182334E-4 0.0 0.0 0.20505940424947208 0.0 60-61 3.8168339553182334E-4 0.0 0.0 0.25787484410618816 0.0 62-63 3.8168339553182334E-4 0.0 0.0 0.34599599804959785 0.0 64-65 3.8168339553182334E-4 0.0 0.0 0.40329621780381286 0.0 66-67 3.8168339553182334E-4 0.0 0.0 0.5133641669903024 0.0 68-69 3.8168339553182334E-4 0.0 0.0 0.6533942627260401 0.0 70-71 3.8168339553182334E-4 0.0 0.0 0.7397978413895565 0.0 72-73 3.8168339553182334E-4 0.0 0.0 0.8830722459873148 0.0 74-75 3.8168339553182334E-4 0.0 0.0 1.0403258049464261 0.0 76-77 3.8168339553182334E-4 0.0 0.0 1.1409948005179444 0.0 78-79 4.2939381997330125E-4 0.0 0.0 1.241997769060553 0.0 80-81 5.72525093297735E-4 0.0 0.0 1.433364281495321 0.0 82-83 5.72525093297735E-4 0.0 0.0 1.592192284461001 0.0 84-85 5.72525093297735E-4 0.0 0.0 1.827118414410838 0.0 86-87 6.20235517739213E-4 0.0 0.0 2.1904332965326927 0.0 88-89 6.679459421806909E-4 0.0 0.0 2.6776998613535063 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCGATT 35 2.7816402E-4 54.310143 8 TATCACG 60 8.284605E-5 39.58791 2 TACGCTA 60 0.0039393366 31.685457 9 TACCTGG 875 0.0 26.060154 2 GAGTACT 3845 0.0 24.223003 12-13 GTACATG 4850 0.0 23.89853 1 GGCCGAT 120 1.7054641E-4 23.760689 7 GTACCTG 1025 0.0 23.635748 1 GTACACG 145 2.3328637E-5 22.93259 1 TACATGG 4980 0.0 22.79882 2 GAGTACG 190 3.2842217E-7 22.501528 1 ACATGGG 4805 0.0 22.247152 3 GTACTTT 4255 0.0 21.892603 14-15 GTGTAGC 440 0.0 21.592375 1 ATGGGCG 155 3.896767E-5 21.45819 5 AGTACTT 4010 0.0 20.086006 12-13 GTGCGGA 120 0.0047613527 19.793009 1 ACTTTTT 4990 0.0 19.33419 16-17 GGGACGC 150 7.699577E-4 19.007643 6 CATGGGA 2480 0.0 18.58344 4 >>END_MODULE