FastQCFastQC Report
Thu 26 May 2016
SRR936495_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936495_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1047989
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT78980.7536338644775852No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53090.5065892867196126No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48600.4637453255711654No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT44980.4292029782755354No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC30190.2880755427776437No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29200.27862887873823106No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT28630.27318989035190255No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT22620.2158419601732461No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC21080.20114714944527093No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT20410.19475395257011285No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15400.146948107279752No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA14530.13864649342693483No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG14180.13530676371603137No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC14140.13492508032049955No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA14000.13358918843613818No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA12610.12032569044140731No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC12490.11918064025481184No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA11750.11211949743747311No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG9000.027.9803032
TATCACG950.001224419125.0072212
GTACCTG10550.023.8717331
GTACATG42050.022.8268451
TACACCG1252.2549511E-422.8011465
ATCACGC1050.002194234622.6223433
GAGTACT56800.022.41202712-13
TACATGG42600.021.972312
ACCGTCC1302.9421673E-421.9220878
ACATGGG41550.021.3809743
TCACGCA1150.003720949420.6541964
GTACTTT61800.020.56032614-15
GTAAGGT3302.7284841E-1120.153494
AGTACTT58500.020.13680312-13
TAAGGTG3553.6379788E-1220.071435
GCGACAG1951.0329066E-519.48637
CTGGTCG2051.5904478E-518.5348639
CATGGGA23850.017.9262854
GTATAAC3451.0768417E-917.9054321
ACTTTTT72650.017.68502216-17
Produced by FastQC (version 0.11.5)