FastQCFastQC Report
Thu 26 May 2016
SRR936494_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936494_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1098968
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT76310.6943787262231476No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44780.4074731930320082No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT43940.39982965837039836No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36740.3343136469851715No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT28950.2634289624447663No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC27570.25087172692926457No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT22150.20155272946982988No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20030.18226190389529084No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC18410.1675208013336148No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC17070.15532754365914203No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT16770.15259770985142426No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG14660.13339787873714248No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA14300.13012207816788113No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC13280.12084064322164068No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA13010.11838379279469466No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA12280.1117411971959147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTG1051.8938226E-631.683241
TACCTGG10550.031.531652
GTACCTG10400.031.5309181
GTACATG48850.024.419121
TACATGG49450.023.7373242
GAGTACT43100.023.59166112-13
ACATGGG48550.022.6112123
GTACTTT46750.021.80006614-15
GGTATGC1604.9741888E-520.789298
GTATAGG3001.6552804E-1020.5941071
CATGGGG20800.020.3342324
ACCTGGG16750.019.8602313
ATTCGCG1452.4392648E-919.6565180-81
AGTACTT44800.019.62075412-13
GTGTAGC5500.019.0099451
ACGTGGG2001.2793673E-519.0090793
CATGGGT8400.018.669634
ACTTTTT55700.018.6383916-17
TAGACAG5900.018.5266425
GTATATA5150.018.4562571
Produced by FastQC (version 0.11.5)