##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936494_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1098968 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.409860887669158 31.0 31.0 34.0 30.0 34.0 2 31.437494085360083 31.0 31.0 34.0 28.0 34.0 3 31.48816890027735 31.0 31.0 34.0 28.0 34.0 4 35.129518784896376 37.0 35.0 37.0 32.0 37.0 5 35.05778694193098 37.0 35.0 37.0 32.0 37.0 6 35.18482521784074 37.0 35.0 37.0 32.0 37.0 7 35.02246562229291 37.0 35.0 37.0 32.0 37.0 8 35.016600119384734 37.0 35.0 37.0 32.0 37.0 9 36.52192693508819 39.0 37.0 39.0 32.0 39.0 10-11 36.40095162006537 39.0 37.0 39.0 32.0 39.0 12-13 36.31032477742755 39.0 35.0 39.0 32.0 39.0 14-15 37.37756285897315 40.0 37.0 41.0 32.0 41.0 16-17 37.32809190076509 40.0 37.0 41.0 32.0 41.0 18-19 37.32125321210445 40.0 37.0 41.0 32.0 41.0 20-21 37.2397767723901 39.5 37.0 41.0 31.0 41.0 22-23 37.143560595030976 39.0 36.0 41.0 31.0 41.0 24-25 36.983182858827554 39.0 36.0 41.0 31.0 41.0 26-27 36.77017074200523 39.0 36.0 40.5 30.0 41.0 28-29 36.89581452781155 39.0 36.0 41.0 30.0 41.0 30-31 36.841594113750354 39.0 36.0 41.0 30.0 41.0 32-33 36.69746343842587 39.0 35.5 41.0 30.0 41.0 34-35 36.510924339926184 39.0 35.0 41.0 30.0 41.0 36-37 36.261621357491755 39.0 35.0 40.0 30.0 41.0 38-39 36.08889567303143 39.0 35.0 40.0 29.5 41.0 40-41 35.96629565192071 38.5 35.0 40.0 29.0 41.0 42-43 35.70982367093491 38.0 35.0 40.0 28.0 41.0 44-45 35.43931579445443 38.0 35.0 40.0 27.0 41.0 46-47 35.0793371599537 38.0 34.0 40.0 25.5 41.0 48-49 34.804056624032725 38.0 34.0 40.0 24.0 41.0 50-51 34.07142337174513 37.5 33.5 39.5 23.5 40.5 52-53 34.25174072402472 38.0 33.5 39.5 24.0 40.5 54-55 34.61228488909595 38.0 34.0 40.0 24.0 41.0 56-57 34.52562949967606 38.0 34.0 40.0 24.0 41.0 58-59 34.288757270457374 38.0 34.0 40.0 23.0 41.0 60-61 33.95163143967795 37.5 33.0 40.0 22.5 41.0 62-63 33.6643328104185 37.0 33.0 40.0 22.0 41.0 64-65 33.36014105961229 36.5 33.0 39.5 22.0 41.0 66-67 32.97186906261147 36.0 32.0 39.0 20.5 41.0 68-69 32.58965957152529 35.5 32.0 39.0 19.5 40.5 70-71 32.1675144317214 35.0 31.5 38.5 18.5 40.0 72-73 31.74244791477095 35.0 31.0 37.5 17.0 40.0 74-75 31.32754911880965 35.0 31.0 37.0 14.0 39.0 76-77 30.91292512611832 35.0 31.0 36.5 10.0 39.0 78-79 30.450356152317447 34.5 30.0 36.0 7.0 38.5 80-81 30.016999130092962 34.0 30.0 36.0 6.5 37.5 82-83 29.606656881729037 34.0 29.0 35.0 2.0 37.0 84-85 29.22652797897664 34.0 29.0 35.0 2.0 36.5 86-87 28.924265310727883 34.0 29.0 35.0 2.0 36.0 88-89 28.537537489717625 34.0 29.0 35.0 2.0 36.0 90-91 28.18371872520401 34.0 28.5 35.0 2.0 35.5 92-93 27.81910073814706 34.0 27.0 35.0 2.0 35.0 94-95 27.39994704122413 34.0 26.5 35.0 2.0 35.0 96-97 26.83702619184544 33.0 25.0 35.0 2.0 35.0 98-99 26.213803313654264 33.0 25.0 35.0 2.0 35.0 100-101 24.147492920630995 31.0 12.5 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.46913248655710404 1101 2 0.37901312926659614 1101 3 0.4961542435095012 1101 4 0.3553018102675125 1101 5 0.44668150257960093 1101 6 0.3856827708885362 1101 7 0.33270690244413714 1101 8 0.28553712172420376 1101 9 0.5213263558662078 1101 10-11 0.40770128337430833 1101 12-13 0.5762048199908918 1101 14-15 0.7037027329382326 1101 16-17 0.8640863250371069 1101 18-19 0.8906779509692839 1101 20-21 0.692242634269661 1101 22-23 0.7705574236625381 1101 24-25 0.7683041925570606 1101 26-27 0.751785980278477 1101 28-29 0.8509133291003508 1101 30-31 0.6038754942175117 1101 32-33 0.6049472373925866 1101 34-35 0.7413719098869009 1101 36-37 0.7821958590790317 1101 38-39 0.7863425272388511 1101 40-41 0.5790378151223194 1101 42-43 0.5583021448508845 1101 44-45 0.40378466960915915 1101 46-47 0.5705176306288351 1101 48-49 0.5975700931860004 1101 50-51 0.6841219275536474 1101 52-53 0.8964526464301414 1101 54-55 0.9850651826001879 1101 56-57 0.8761209412416733 1101 58-59 0.7777066490663955 1101 60-61 0.8103026523192298 1101 62-63 1.028443000746634 1101 64-65 1.0735833339773748 1101 66-67 1.0323001360427213 1101 68-69 0.9359997317622444 1101 70-71 0.8407430104976541 1101 72-73 0.8925330778285101 1101 74-75 0.8584906947292161 1101 76-77 0.7515843702653839 1101 78-79 0.9034501681063922 1101 80-81 0.8484024208061562 1101 82-83 0.9572945987841344 1101 84-85 0.9279569409298425 1101 86-87 0.8803549593283364 1101 88-89 0.8419865471377541 1101 90-91 0.9603446783595402 1101 92-93 0.7538274652808639 1101 94-95 0.7466729608787439 1101 96-97 0.5657128379214029 1101 98-99 0.5641075934235538 1101 100-101 0.3532618279463371 1102 1 0.1284674153911496 1102 2 0.20625390599579063 1102 3 0.35943023473627633 1102 4 0.27508297944125104 1102 5 0.202937358209077 1102 6 0.10750364114568356 1102 7 -0.016228158245013447 1102 8 0.0013873608128918136 1102 9 0.29172286008496684 1102 10-11 0.28580320493269085 1102 12-13 0.44966107848092207 1102 14-15 0.4815488475022107 1102 16-17 0.4080277792123965 1102 18-19 0.41703985133086263 1102 20-21 0.18968963047949217 1102 22-23 -0.17111662270154682 1102 24-25 0.022155313832001866 1102 26-27 0.0772961588570169 1102 28-29 0.13601890436886777 1102 30-31 0.03419392562820178 1102 32-33 0.11341244271029183 1102 34-35 0.19617202309854775 1102 36-37 -0.1577188809293304 1102 38-39 -0.027529682745786488 1102 40-41 -0.23427103855567566 1102 42-43 -0.043112798141372366 1102 44-45 0.16798796963421836 1102 46-47 0.02818214721980894 1102 48-49 0.1620862113232988 1102 50-51 0.1236857519729142 1102 52-53 0.02799904598215619 1102 54-55 0.11434532266920883 1102 56-57 0.23494716068179144 1102 58-59 0.2757538156341113 1102 60-61 -0.12251406842018042 1102 62-63 0.032419256021348986 1102 64-65 -0.00909325694691887 1102 66-67 -0.07730407575493814 1102 68-69 -0.057530789788160064 1102 70-71 0.20109110255034324 1102 72-73 -0.17048616072181488 1102 74-75 -0.21520461867319796 1102 76-77 -0.12012575386889068 1102 78-79 -0.1263104459925124 1102 80-81 0.07711629997365677 1102 82-83 0.10202346836309673 1102 84-85 -0.007170283702741642 1102 86-87 -0.2782426752506666 1102 88-89 -0.3741784689759946 1102 90-91 -0.6072925282848196 1102 92-93 -0.5751233072400446 1102 94-95 -0.38053275806590037 1102 96-97 -0.36891047327621607 1102 98-99 -0.6339918558517503 1102 100-101 -0.41579754940309144 1103 1 0.29627959951970695 1103 2 0.4409096447997598 1103 3 0.2872194440520346 1103 4 0.22301962981152457 1103 5 0.3173743592611231 1103 6 0.20007395799697747 1103 7 0.2995092017401362 1103 8 0.04294443942749382 1103 9 0.5858043317106834 1103 10-11 0.5824100365229299 1103 12-13 0.40308551171730045 1103 14-15 0.5900851765861077 1103 16-17 0.5979496751630577 1103 18-19 0.4683447871767328 1103 20-21 0.2069371782856706 1103 22-23 0.38071755897222914 1103 24-25 0.4693351620074182 1103 26-27 0.42455813360793826 1103 28-29 0.5206004456152371 1103 30-31 0.5327692809513493 1103 32-33 0.6250065363010648 1103 34-35 0.6424912827474571 1103 36-37 0.6391377469560595 1103 38-39 0.47910232831100075 1103 40-41 0.45273194739642975 1103 42-43 0.6629411037089881 1103 44-45 0.6523526554646182 1103 46-47 0.4199149534990738 1103 48-49 0.6093047376347513 1103 50-51 0.3079744997295819 1103 52-53 0.6651162613626553 1103 54-55 0.7388193000192871 1103 56-57 0.8669092251659762 1103 58-59 0.7001770601624386 1103 60-61 0.9751356546443617 1103 62-63 0.8005741284350663 1103 64-65 0.6113172598961185 1103 66-67 0.6134597142898315 1103 68-69 0.3474560467528818 1103 70-71 0.5465958006546003 1103 72-73 0.5844482024813544 1103 74-75 0.5063443891036847 1103 76-77 0.5125167234915047 1103 78-79 0.635166001601192 1103 80-81 0.8816495709062657 1103 82-83 0.8828167440402197 1103 84-85 0.7639221931419478 1103 86-87 0.8649025276290523 1103 88-89 0.8306606170854387 1103 90-91 0.7582187579952695 1103 92-93 0.7692445759829241 1103 94-95 0.6723131791826908 1103 96-97 0.6424895849988417 1103 98-99 0.5519258860670995 1103 100-101 0.6564835065615959 1104 1 0.24659062597023507 1104 2 0.21642102338434555 1104 3 0.29173369131489935 1104 4 0.13184676032373943 1104 5 0.27659435197558935 1104 6 0.2897713807399782 1104 7 0.4732691434558802 1104 8 0.41681239927611813 1104 9 0.6617825661833905 1104 10-11 0.61255424199517 1104 12-13 0.513665596917555 1104 14-15 0.6374888836065296 1104 16-17 0.3569686833188044 1104 18-19 0.5171480499820618 1104 20-21 0.5768401516934816 1104 22-23 0.5304678144717982 1104 24-25 0.5236439977389082 1104 26-27 0.3888495397156433 1104 28-29 0.36632455920882734 1104 30-31 0.21254105153803238 1104 32-33 0.08077886595116013 1104 34-35 0.3467094287818995 1104 36-37 0.36792432639871464 1104 38-39 0.41913835121994936 1104 40-41 0.29858033243679927 1104 42-43 0.32227417395365876 1104 44-45 0.08277740451850946 1104 46-47 0.3869321099153993 1104 48-49 0.19651230446938683 1104 50-51 0.18458813126379425 1104 52-53 0.3183597145025061 1104 54-55 0.5065701955326176 1104 56-57 0.3223232862430194 1104 58-59 0.373200543355118 1104 60-61 0.33775499317875557 1104 62-63 0.27626688641373676 1104 64-65 0.20140963184277183 1104 66-67 0.17940579629254216 1104 68-69 0.3829847158278241 1104 70-71 0.39154993721785303 1104 72-73 0.22166759565724448 1104 74-75 0.1313361675830329 1104 76-77 0.15847053372363007 1104 78-79 0.22250448034160186 1104 80-81 0.5115094382488685 1104 82-83 0.27792247279581517 1104 84-85 0.3711662382313001 1104 86-87 0.3771614473465341 1104 88-89 0.32027146089172476 1104 90-91 0.24934425887560607 1104 92-93 0.3211682220882004 1104 94-95 0.3485661389301278 1104 96-97 0.5163230777031913 1104 98-99 0.6732075452458588 1104 100-101 0.7229494416394076 1105 1 -0.04196063247052351 1105 2 0.14299297813309408 1105 3 0.20148989582248333 1105 4 0.2943566261485202 1105 5 0.3743493287360877 1105 6 0.13448329865631337 1105 7 0.2973724496033867 1105 8 0.11689132587438422 1105 9 0.2809661143724682 1105 10-11 0.35339217938006584 1105 12-13 0.09781231269409574 1105 14-15 0.3285657931917214 1105 16-17 0.2553901513535308 1105 18-19 0.18227564798259266 1105 20-21 -0.05770338947989728 1105 22-23 -0.20870170232202412 1105 24-25 -0.049452994280734686 1105 26-27 -0.23421018141038275 1105 28-29 -0.043856971967180414 1105 30-31 -0.2416354809534127 1105 32-33 -0.33079703512749603 1105 34-35 -0.2489693399631676 1105 36-37 -0.18333630676799118 1105 38-39 -0.2753543261456528 1105 40-41 -0.5857066972922169 1105 42-43 -0.5184081026402225 1105 44-45 -0.22625311376615542 1105 46-47 -0.18380146896734573 1105 48-49 0.006481172311175953 1105 50-51 0.02215719203727673 1105 52-53 0.04531314780953721 1105 54-55 -0.020124533924544608 1105 56-57 0.14847210672886035 1105 58-59 0.04036707847745902 1105 60-61 0.319904427538134 1105 62-63 0.2230023946133386 1105 64-65 0.10529238199624302 1105 66-67 -0.034487477407360245 1105 68-69 0.09186615053798164 1105 70-71 0.17755581286461108 1105 72-73 0.014702323360474168 1105 74-75 -0.099905610896311 1105 76-77 0.03743659528637622 1105 78-79 0.13979817498336544 1105 80-81 0.1473508834825772 1105 82-83 0.15508856943704785 1105 84-85 0.09679765101740756 1105 86-87 0.20913329685981807 1105 88-89 0.08103836433818756 1105 90-91 0.008054685956196295 1105 92-93 -0.1224869563736064 1105 94-95 0.03521000457951473 1105 96-97 0.11543296106722067 1105 98-99 -0.023764742748529244 1105 100-101 -0.0038110599829721536 1106 1 -0.0016756831494326718 1106 2 0.06387651226964053 1106 3 0.03360884782095397 1106 4 0.21902673515598536 1106 5 0.25637266015701243 1106 6 0.25562500835224 1106 7 0.3251502273811653 1106 8 0.2985321749851124 1106 9 0.4486750453344115 1106 10-11 0.42810531764742166 1106 12-13 0.4476927869721621 1106 14-15 0.3762326510995493 1106 16-17 0.29164947435983635 1106 18-19 0.2765670995029623 1106 20-21 0.3043161171179207 1106 22-23 0.2952091792996967 1106 24-25 0.11535385215261584 1106 26-27 0.33782079965674683 1106 28-29 0.13118605729271593 1106 30-31 0.14406446167424036 1106 32-33 0.16794077324886558 1106 34-35 0.09906565716821802 1106 36-37 0.15135005773765187 1106 38-39 0.07802108024180399 1106 40-41 -0.07760285334499883 1106 42-43 0.22328246917083305 1106 44-45 0.23208021956718028 1106 46-47 0.1352126828781266 1106 48-49 0.21353318990536962 1106 50-51 0.49123349725815757 1106 52-53 0.4094841174021866 1106 54-55 0.6436926395311815 1106 56-57 0.7156503434854073 1106 58-59 0.5981410314321352 1106 60-61 0.36772427837003363 1106 62-63 0.5052408723337365 1106 64-65 0.5433637149470982 1106 66-67 0.5815146262453439 1106 68-69 0.4781876269196985 1106 70-71 0.6065336288906238 1106 72-73 0.5394441478010918 1106 74-75 0.40270483087075704 1106 76-77 0.30872173584862495 1106 78-79 0.2627088653639369 1106 80-81 0.23801831511386595 1106 82-83 0.37678009745387797 1106 84-85 0.297270972876273 1106 86-87 0.5343341000726696 1106 88-89 0.6271513878608488 1106 90-91 0.5010313545352751 1106 92-93 0.6128352849727285 1106 94-95 0.5049939020742578 1106 96-97 0.4114838312144755 1106 98-99 0.7341287359280848 1106 100-101 0.9833853446833096 1107 1 0.3433605364955028 1107 2 0.44133150357442474 1107 3 0.5501547823378381 1107 4 0.3546295826831596 1107 5 0.5635052403219092 1107 6 0.4682987563035468 1107 7 0.5847556271734788 1107 8 0.4423623242425734 1107 9 0.5214571394429299 1107 10-11 0.7351715889632118 1107 12-13 0.7394567274693529 1107 14-15 0.7542518931768925 1107 16-17 0.8137791144153894 1107 18-19 0.7985566151981445 1107 20-21 0.9956121423403061 1107 22-23 0.8386577398972292 1107 24-25 1.0034582948455082 1107 26-27 1.0207594076713562 1107 28-29 0.8788696264603573 1107 30-31 0.8369619081579955 1107 32-33 0.9189285248784316 1107 34-35 0.6874903841133815 1107 36-37 0.7454836012575257 1107 38-39 0.8348815439033004 1107 40-41 0.9144676091904955 1107 42-43 0.9909525263465753 1107 44-45 0.7149462631808845 1107 46-47 0.8003292238082054 1107 48-49 0.5419321439771068 1107 50-51 0.43981035922315215 1107 52-53 0.19326597379973975 1107 54-55 0.6312328314515128 1107 56-57 0.5547137622695857 1107 58-59 0.5544747776318886 1107 60-61 0.36497934223921646 1107 62-63 0.41423885804605476 1107 64-65 0.3026926193497488 1107 66-67 0.2792901520244726 1107 68-69 0.4354909836487977 1107 70-71 0.5498633340039092 1107 72-73 0.5965410713207149 1107 74-75 0.5198763205678638 1107 76-77 0.7868135423268754 1107 78-79 0.5994777477470485 1107 80-81 0.7954743073069359 1107 82-83 0.7130510379135373 1107 84-85 0.8348743458698031 1107 86-87 0.8493461732876497 1107 88-89 0.8991402780008464 1107 90-91 0.8066713600401627 1107 92-93 0.7128490471866975 1107 94-95 0.9244549237309805 1107 96-97 1.0732211230609892 1107 98-99 1.1194669736140277 1107 100-101 1.4610680380736945 1108 1 0.021104991789130167 1108 2 -0.09718115122014126 1108 3 -0.04460700064754519 1108 4 -0.1087350582864417 1108 5 0.0596964028891378 1108 6 -0.046087063817033425 1108 7 0.10562286419708045 1108 8 -0.15189865280365922 1108 9 0.24165967040184455 1108 10-11 0.07549150418461181 1108 12-13 0.08867200234110584 1108 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-2.1818437239370567 2316 72-73 -2.1774812048550807 2316 74-75 -2.2921060853921595 2316 76-77 -3.130534934962732 2316 78-79 -3.1748674184188808 2316 80-81 -3.234688883788504 2316 82-83 -2.9691813283203956 2316 84-85 -3.0836802340868132 2316 86-87 -3.2036484350500274 2316 88-89 -3.6886913424911647 2316 90-91 -3.5551238713504745 2316 92-93 -3.704796451939334 2316 94-95 -3.832802479180298 2316 96-97 -4.262068027465798 2316 98-99 -4.884172583227542 2316 100-101 -5.40175914787768 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2317.0 3 870.0 4 875.0 5 955.0 6 1161.0 7 1346.0 8 1659.0 9 2149.0 10 2576.0 11 3475.0 12 4365.0 13 5423.0 14 5813.0 15 7196.0 16 8813.0 17 10959.0 18 9900.0 19 6956.0 20 7054.0 21 7572.0 22 8269.0 23 9330.0 24 10902.0 25 12398.0 26 14471.0 27 17516.0 28 20494.0 29 24840.0 30 30010.0 31 37079.0 32 46302.0 33 59869.0 34 77928.0 35 106513.0 36 149392.0 37 196325.0 38 160288.0 39 25602.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.30116073980098 18.524147647121993 14.192078137428535 21.982613475648492 2 15.427974453850032 20.804387036184313 39.670517274956005 24.097121235009656 3 20.414239953407108 24.76212143273151 29.657014414676762 25.16662419918462 4 12.679424312955872 16.606665131355573 37.92248742243417 32.79142313325438 5 13.558373078985436 37.66331070433331 33.828883246558995 14.949432970122256 6 30.777600629357448 36.28903095160267 17.761683676860642 15.171684742179242 7 28.43458505362955 32.98599733490617 20.75523734626559 17.82418026519868 8 26.301063387274848 33.319346585246194 19.790854123071856 20.5887359044071 9 26.158026987942158 17.92349199617396 21.075786298891412 34.84269471699247 10-11 25.07211497975045 26.413360287212672 27.054918453286746 21.459606279750133 12-13 27.019272786861364 23.972679436291468 27.245593751137974 21.76245402570919 14-15 23.165013197415128 25.69290980249386 26.783971966869935 24.35810503322108 16-17 21.85015450569555 29.007386236933 27.1280599636832 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21.912812824881765 27.14199593705853 28.394228166239365 22.550963071820338 44-45 22.356708386546693 26.785000134218233 28.339474765379702 22.518816713855372 46-47 22.88589651309761 27.316161650170486 27.989218711468588 21.80872312526332 48-49 22.789959980417883 27.196390776023193 27.930310602186424 22.083338641372496 50-51 21.974369135587338 27.550608604497846 28.531819279975355 21.94320297993946 52-53 22.545155418172456 28.07677660904068 27.366783611150414 22.011284361636445 54-55 21.951270559865325 27.315512887594696 27.87724366995018 22.855972882589803 56-57 22.617607902981725 27.528989641714148 27.513292511695497 22.34010994360863 58-59 23.354773603216543 27.15997031697233 27.281996497548327 22.2032595822628 60-61 22.527212041908637 27.241433279767634 28.616230588500784 21.61512408982294 62-63 22.19299742254556 27.512062623270793 28.232430428066728 22.062509526116916 64-65 21.876696242230555 28.18948137585748 27.74527918583103 22.188543196080936 66-67 22.10996676773449 27.79171049710082 27.29663891851361 22.801683816651074 68-69 21.824386426302905 27.687757646352228 27.772843505719408 22.71501242162546 70-71 22.602670539704555 27.58174466888529 27.366434338994555 22.449150452415598 72-73 22.409803336330185 27.189898920042367 28.088180160373888 22.31211758325356 74-75 21.836404411359904 28.632162670147608 27.34255104143965 22.18888187705284 76-77 22.216964585112525 27.71549247199891 27.299906590999157 22.767636351889404 78-79 22.253672541640952 27.80749855719953 27.48152578197965 22.45730311917987 80-81 22.587710615834443 27.918632035223045 27.252534216184856 22.24112313275766 82-83 22.352819132454933 27.772916982315042 27.732331994301724 22.141931890928298 84-85 22.106744116524236 28.03087559260198 27.437033693509143 22.425346597364637 86-87 22.23311705448225 28.08970870605793 27.235847082055532 22.441327157404288 88-89 22.181368311277986 27.921552604326866 26.79206153050955 23.105017553885606 90-91 22.233451582899985 27.43241300102111 27.345864511114794 22.988270904964114 92-93 22.667718116254953 27.822202483526354 27.348413110478198 22.161666289740495 94-95 22.177388531554058 27.595333777423477 27.529493773637792 22.697783917384672 96-97 21.52494259102286 27.616368349283178 28.12427911751016 22.734409942183802 98-99 21.886166504355106 28.17454105268906 26.91870466274085 23.020587780214978 100-101 21.55157816926539 27.711681875450616 27.391560035248453 23.345179920035537 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 143.0 1 113.5 2 170.0 3 250.5 4 214.5 5 208.5 6 316.5 7 589.5 8 981.0 9 1273.5 10 1250.0 11 1115.0 12 1171.5 13 1256.5 14 1393.0 15 1498.5 16 1536.0 17 1665.0 18 1779.5 19 1871.5 20 2026.5 21 2329.0 22 2763.0 23 3323.5 24 3864.0 25 4648.0 26 6004.5 27 7538.5 28 8778.5 29 10330.5 30 12628.5 31 15121.5 32 17735.5 33 19927.0 34 22221.0 35 24145.5 36 26094.5 37 28139.0 38 28954.0 39 29492.5 40 30540.0 41 32888.0 42 35036.0 43 36507.5 44 38053.0 45 39880.0 46 40789.0 47 45720.5 48 58837.0 49 57625.0 50 44287.5 51 37805.5 52 34857.0 53 34100.5 54 33090.5 55 30461.5 56 27290.5 57 24495.0 58 22479.0 59 20038.0 60 16823.5 61 13902.0 62 11432.5 63 8906.5 64 6649.5 65 5207.0 66 3998.5 67 3210.0 68 2407.0 69 1596.5 70 1192.5 71 958.0 72 774.0 73 646.0 74 515.0 75 385.0 76 274.5 77 184.0 78 115.0 79 69.0 80 43.0 81 27.5 82 21.0 83 14.0 84 10.0 85 6.5 86 3.0 87 3.0 88 5.0 89 5.5 90 3.0 91 2.0 92 1.5 93 1.5 94 1.0 95 0.0 96 0.0 97 1.5 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01692496960785027 2 0.008553479264182396 3 0.00800751250263884 4 0.03503286719904492 5 0.047772091635061256 6 0.06415109448136798 7 0.02911822728232305 8 0.028117288219493195 9 0.016469997306563976 10-11 0.049910461451106856 12-13 0.048045075015833036 14-15 0.024386515348945557 16-17 0.02757132145794964 18-19 0.03421391705672958 20-21 0.024932482110489117 22-23 0.016333505616178086 24-25 0.004140247941705309 26-27 0.0030938116487468246 28-29 0.0020928725859169694 30-31 0.008234998653281988 32-33 0.0013194196737302633 34-35 0.002183867046174229 36-37 0.0059601371468504995 38-39 0.00805300973276747 40-41 0.0046407174731202365 42-43 9.554418327012252E-4 44-45 0.0010464362929584847 46-47 0.002456850426946007 48-49 5.459667615435572E-4 50-51 0.001774391975016561 52-53 0.0012284252134730036 54-55 0.00391276179106216 56-57 0.003730772870547641 58-59 0.002138369816045599 60-61 5.459667615435572E-4 62-63 0.0014104141339875227 64-65 0.002866325498103676 66-67 0.0032758005692613435 68-69 0.007097567900066244 70-71 0.008098506962896098 72-73 0.0032758005692613435 74-75 0.0029573199583609352 76-77 0.0034122922596472325 78-79 0.03730772870547641 80-81 0.023249084595729812 82-83 0.0035942811801617514 84-85 0.00969091001739814 86-87 0.007097567900066244 88-89 0.0060056343769791295 90-91 0.014741102561676047 92-93 0.015241572093090974 94-95 0.008371490343667878 96-97 0.004959198084020644 98-99 0.023476570746372962 100-101 0.043677340923484576 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1098968.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.521395925634415 #Duplication Level Percentage of deduplicated Percentage of total 1 84.79748336662833 53.01657031062369 2 9.074394461037585 11.346876177678297 3 2.464661474641253 4.622822276361113 4 1.0743376312503796 2.6867635360485287 5 0.5876604246662953 1.8370675040193956 6 0.3649217370036439 1.3689249840645032 7 0.2782460275729595 1.21774310382368 8 0.18897877870640986 0.945217363603704 9 0.15043397950972043 0.8464808154236405 >10 0.9135939566970831 10.818453565450554 >50 0.06850198348382756 2.963162922260859 >100 0.03255285086232388 3.6432786102803143 >500 0.0016057450808066968 0.6554777916624444 >1k 0.0024816060339739863 3.3347044767143403 >5k 1.4597682552788154E-4 0.6964565619849782 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7631 0.6943787262231476 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4478 0.4074731930320082 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4394 0.39982965837039836 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3674 0.3343136469851715 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2895 0.2634289624447663 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2757 0.25087172692926457 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2215 0.20155272946982988 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2003 0.18226190389529084 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1841 0.1675208013336148 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1707 0.15532754365914203 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1677 0.15259770985142426 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1466 0.13339787873714248 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1430 0.13012207816788113 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1328 0.12084064322164068 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1301 0.11838379279469466 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1228 0.1117411971959147 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 9.099446025725954E-5 0.0 2 0.0 0.0 0.0 9.099446025725954E-5 0.0 3 0.0 0.0 0.0 9.099446025725954E-5 0.0 4 0.0 0.0 0.0 9.099446025725954E-5 0.0 5 0.0 0.0 0.0 9.099446025725954E-5 0.0 6 0.0 0.0 0.0 9.099446025725954E-5 0.0 7 0.0 0.0 0.0 9.099446025725954E-5 0.0 8 0.0 0.0 0.0 9.099446025725954E-5 0.0 9 0.0 0.0 0.0 9.099446025725954E-5 0.0 10-11 0.0 0.0 0.0 9.099446025725954E-5 0.0 12-13 9.099446025725954E-5 0.0 0.0 9.099446025725954E-5 0.0 14-15 9.099446025725954E-5 0.0 0.0 9.099446025725954E-5 0.0 16-17 9.099446025725954E-5 0.0 0.0 9.099446025725954E-5 0.0 18-19 9.099446025725954E-5 0.0 0.0 9.099446025725954E-5 0.0 20-21 9.099446025725954E-5 0.0 0.0 9.099446025725954E-5 0.0 22-23 9.099446025725954E-5 0.0 0.0 1.8198892051451908E-4 0.0 24-25 9.099446025725954E-5 0.0 0.0 1.8198892051451908E-4 0.0 26-27 9.099446025725954E-5 0.0 0.0 4.5497230128629766E-4 0.0 28-29 9.099446025725954E-5 0.0 0.0 8.644473724439656E-4 0.0 30-31 9.099446025725954E-5 0.0 0.0 0.0019108836654024503 0.0 32-33 9.099446025725954E-5 0.0 0.0 0.0032303033391327136 0.0 34-35 9.099446025725954E-5 0.0 0.0 0.004413231322477088 0.0 36-37 9.099446025725954E-5 0.0 0.0 0.0061421260673650184 0.0 38-39 9.099446025725954E-5 0.0 0.0 0.00964541278726951 0.0 40-41 9.099446025725954E-5 0.0 0.0 0.012102263214215519 0.0 42-43 2.2748615064314883E-4 0.0 0.0 0.018881350503381356 0.0 44-45 2.729833807717786E-4 0.0 0.0 0.026797868545762934 0.0 46-47 3.6397784102903816E-4 0.0 0.0 0.03412292259647233 0.0 48-49 3.6397784102903816E-4 0.0 0.0 0.044405296605542655 0.0 50-51 3.6397784102903816E-4 0.0 0.0 0.0566895487402727 0.0 52-53 3.6397784102903816E-4 0.0 0.0 0.07889219704304401 0.0 54-55 3.6397784102903816E-4 0.0 0.0 0.09381528852523459 0.0 56-57 3.6397784102903816E-4 0.0 0.0 0.13025856985826703 0.0 58-59 3.6397784102903816E-4 0.0 0.0 0.1791225950164154 0.0 60-61 3.6397784102903816E-4 0.0 0.0 0.22143501903604107 0.0 62-63 3.6397784102903816E-4 0.0 0.0 0.2935936260200479 0.0 64-65 3.6397784102903816E-4 0.0 0.0 0.3430946123999971 0.0 66-67 3.6397784102903816E-4 0.0 0.0 0.443506999293883 0.0 68-69 3.6397784102903816E-4 0.0 0.0 0.5631192173020506 0.0 70-71 3.6397784102903816E-4 0.0 0.0 0.6410104752822648 0.0 72-73 3.6397784102903816E-4 0.0 0.0 0.7758187681533948 0.0 74-75 3.6397784102903816E-4 0.0 0.0 0.9113550167065829 0.0 76-77 3.6397784102903816E-4 0.0 0.0 0.9985277096330376 0.0 78-79 3.6397784102903816E-4 0.0 0.0 1.093480428911488 0.0 80-81 7.279556820580763E-4 0.0 0.0 1.2659604283291235 0.0 82-83 7.279556820580763E-4 0.0 0.0 1.4063648805060749 0.0 84-85 7.279556820580763E-4 0.0 0.0 1.6286643469145599 0.0 86-87 7.279556820580763E-4 0.0 0.0 1.9746252848126606 0.0 88-89 7.279556820580763E-4 0.0 0.0 2.4353757343252944 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACGTG 105 1.8938226E-6 31.68324 1 TACCTGG 1055 0.0 31.53165 2 GTACCTG 1040 0.0 31.530918 1 GTACATG 4885 0.0 24.41912 1 TACATGG 4945 0.0 23.737324 2 GAGTACT 4310 0.0 23.591661 12-13 ACATGGG 4855 0.0 22.611212 3 GTACTTT 4675 0.0 21.800066 14-15 GGTATGC 160 4.9741888E-5 20.78929 8 GTATAGG 300 1.6552804E-10 20.594107 1 CATGGGG 2080 0.0 20.334232 4 ACCTGGG 1675 0.0 19.860231 3 ATTCGCG 145 2.4392648E-9 19.65651 80-81 AGTACTT 4480 0.0 19.620754 12-13 GTGTAGC 550 0.0 19.009945 1 ACGTGGG 200 1.2793673E-5 19.009079 3 CATGGGT 840 0.0 18.66963 4 ACTTTTT 5570 0.0 18.63839 16-17 TAGACAG 590 0.0 18.526642 5 GTATATA 515 0.0 18.456257 1 >>END_MODULE