FastQCFastQC Report
Thu 26 May 2016
SRR936494_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936494_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1098968
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT78570.7149434742412881No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55070.5011064926367282No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50010.4550632957465549No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT42730.38881932867927No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30530.2778060871654134No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC29590.269252607901231No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT28750.2616090732396212No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC22880.20819532506860983No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT22160.20164372393008714No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT20330.18499173770300864No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15900.14468119180904268No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG14980.13630970146537477No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC14120.12848417788325048No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA13970.12711926097939155No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA13000.11829279833443741No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA12830.11674589251006398No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC12770.11619992574852044No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA12350.11237815841771553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG9700.033.2977872
GTACCTG11250.031.1686461
CGTATAG650.005533295729.5593031
GTACATG46600.022.6769351
ATATCGT1100.00287939921.5881796
ATCGCTC1100.00287939921.5881798
GAGTACT60300.021.38119312-13
ACATGGG46650.021.078343
TACATGG48300.020.8481242
AGTACTT62500.020.28666312-13
ACCTGGG16200.019.939383
GTACTTT64350.019.77723714-15
GTGTAGC4950.019.4076251
TATACAG5100.018.6250955
GTGTATC2402.7771293E-618.0127031
CATGGGA26550.017.7120424
ACGTCTA2956.346636E-817.7096255
TACTTTT72200.017.5611714-15
CATGGGG20750.017.3977644
AGAGTAC100200.017.25095210-11
Produced by FastQC (version 0.11.5)