##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936494_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1098968 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.504133878329487 31.0 31.0 34.0 30.0 34.0 2 31.733430818731755 31.0 31.0 34.0 30.0 34.0 3 32.015548223424155 33.0 31.0 34.0 30.0 34.0 4 35.81298090572246 37.0 35.0 37.0 35.0 37.0 5 35.63629878213015 37.0 35.0 37.0 33.0 37.0 6 35.74510813781657 37.0 35.0 37.0 33.0 37.0 7 35.686822546243384 37.0 35.0 37.0 33.0 37.0 8 35.66877743482977 37.0 35.0 37.0 33.0 37.0 9 37.31093899003429 39.0 37.0 39.0 34.0 39.0 10-11 37.19874646031549 39.0 37.0 39.0 33.0 39.0 12-13 37.07727750034578 39.0 37.0 39.0 33.0 39.0 14-15 38.18584617568482 40.0 37.0 41.0 33.0 41.0 16-17 38.11391732971296 40.0 37.0 41.0 33.0 41.0 18-19 38.19664630817276 40.0 37.5 41.0 33.5 41.0 20-21 38.27276226423335 40.0 38.0 41.0 34.0 41.0 22-23 38.19932336519353 40.0 37.5 41.0 33.0 41.0 24-25 38.08391008655393 40.0 37.0 41.0 33.0 41.0 26-27 37.87189026432071 40.0 37.0 41.0 33.0 41.0 28-29 37.790221826295216 40.0 37.0 41.0 33.0 41.0 30-31 37.76289891971376 40.0 37.0 41.0 33.0 41.0 32-33 37.571117630358664 40.0 36.5 41.0 33.0 41.0 34-35 37.375862627483244 39.0 36.0 41.0 32.0 41.0 36-37 37.12660423233434 39.0 36.0 41.0 31.5 41.0 38-39 36.96681250045497 39.0 36.0 41.0 31.0 41.0 40-41 36.746089968042746 39.0 35.0 41.0 31.0 41.0 42-43 36.49656131934688 39.0 35.0 40.5 30.0 41.0 44-45 36.19042228709116 39.0 35.0 40.0 30.0 41.0 46-47 35.7470299408172 39.0 35.0 40.0 28.5 41.0 48-49 35.23819756353234 38.0 35.0 40.0 27.0 41.0 50-51 35.03907711598518 38.0 34.0 40.0 26.0 41.0 52-53 34.78320797329859 38.0 34.0 40.0 26.0 41.0 54-55 34.525076253357696 38.0 34.0 40.0 24.5 41.0 56-57 34.23108907629704 38.0 33.5 40.0 24.0 41.0 58-59 33.86289182214587 37.5 33.0 40.0 23.0 41.0 60-61 33.47169935794309 37.0 33.0 40.0 22.0 41.0 62-63 33.20976952923106 36.5 32.0 39.0 22.0 40.5 64-65 32.78358378041945 36.0 32.0 39.0 20.5 40.0 66-67 32.358945847376816 35.5 31.0 39.0 19.5 40.0 68-69 31.91089867948839 35.0 31.0 38.0 18.5 40.0 70-71 31.41790070320519 35.0 30.5 37.5 17.5 39.5 72-73 30.997463074448028 34.5 30.0 37.0 13.5 39.0 74-75 30.643284426844094 34.0 30.0 36.5 11.0 39.0 76-77 29.205572409751696 32.5 28.5 35.0 8.0 37.0 78-79 29.999277503985557 34.0 29.5 36.0 7.5 37.5 80-81 29.8201044070437 34.0 29.5 35.0 7.0 37.0 82-83 29.47031715209178 34.0 29.0 35.0 2.0 37.0 84-85 29.0227445203136 34.0 29.0 35.0 2.0 36.0 86-87 28.664055732287018 34.0 29.0 35.0 2.0 36.0 88-89 28.246596352214077 33.0 28.5 35.0 2.0 35.0 90-91 27.779486299874065 33.0 27.0 35.0 2.0 35.0 92-93 27.335083460118945 33.0 26.0 35.0 2.0 35.0 94-95 26.81420387126832 32.5 25.5 34.5 2.0 35.0 96-97 26.133022526588583 32.0 24.5 34.0 2.0 35.0 98-99 25.301283112884086 32.0 21.5 34.0 2.0 35.0 100-101 22.52175040583529 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.45168196258174476 1101 2 0.5186710276846469 1101 3 0.302383292809175 1101 4 0.17168162477228321 1101 5 0.24191699032552805 1101 6 0.23742850277115224 1101 7 0.17218021896551505 1101 8 0.23923893423353348 1101 9 0.2890852382577904 1101 10-11 0.33400031968007227 1101 12-13 0.34115694908379623 1101 14-15 0.5415781676375389 1101 16-17 0.49734209030326326 1101 18-19 0.46226110367927475 1101 20-21 0.3155609167143325 1101 22-23 0.19599859814064757 1101 24-25 -0.011627051985293235 1101 26-27 0.2721469823229796 1101 28-29 0.22131762216081086 1101 30-31 0.1254414121692733 1101 32-33 0.0731463440095439 1101 34-35 0.27158602086613826 1101 36-37 0.13193636586937174 1101 38-39 0.2947153064374177 1101 40-41 0.17115260920476771 1101 42-43 0.38666589942178575 1101 44-45 0.3526562790147807 1101 46-47 0.43751793812632656 1101 48-49 0.21165021035754705 1101 50-51 0.2621203123867559 1101 52-53 0.2506860838507592 1101 54-55 0.20106603068987994 1101 56-57 0.24868430638635886 1101 58-59 0.3015891440382745 1101 60-61 0.22632631885698373 1101 62-63 0.17510498851579115 1101 64-65 0.07623101997400994 1101 66-67 0.05014053679342112 1101 68-69 0.026809418564958776 1101 70-71 0.08174330750317793 1101 72-73 0.02231806659981217 1101 74-75 0.1529811572346027 1101 76-77 0.3734025174929343 1101 78-79 0.2189031961791521 1101 80-81 0.06745792031994213 1101 82-83 -0.00388830107369742 1101 84-85 0.10746662002947716 1101 86-87 -0.03993178624370586 1101 88-89 0.20789323396206782 1101 90-91 0.28907195317650647 1101 92-93 0.28811137050508506 1101 94-95 0.013923349070523727 1101 96-97 -0.11592900638312287 1101 98-99 0.15891307547977718 1101 100-101 0.057646676211113856 1102 1 0.4817364136031834 1102 2 0.49925212228460225 1102 3 0.16000207891818974 1102 4 0.10571557340688287 1102 5 0.16012697632267958 1102 6 0.20570830036817256 1102 7 0.08769906404455696 1102 8 0.07951053411816389 1102 9 0.1699171412470193 1102 10-11 0.15759954503137408 1102 12-13 0.13742326125478854 1102 14-15 0.25403746109145686 1102 16-17 0.234411745865593 1102 18-19 0.3771882219901812 1102 20-21 0.22932484610430492 1102 22-23 0.19117595743160365 1102 24-25 0.21818956000068823 1102 26-27 -0.032329201012345266 1102 28-29 0.2263361541515465 1102 30-31 0.29009174960889794 1102 32-33 0.43158584704011815 1102 34-35 0.3756931180072698 1102 36-37 0.33272357862689006 1102 38-39 0.2832870766123605 1102 40-41 0.5679662010875788 1102 42-43 0.3423397552984966 1102 44-45 0.22001660346478502 1102 46-47 0.1992678687200211 1102 48-49 0.15322833754232335 1102 50-51 0.2988519541651016 1102 52-53 0.23837262807862203 1102 54-55 0.22670786171945423 1102 56-57 0.14907798031382669 1102 58-59 0.2102875950786256 1102 60-61 0.20934693540115035 1102 62-63 -0.018390002409034878 1102 64-65 0.15273872424302937 1102 66-67 0.15899732202834826 1102 68-69 0.31116452757845536 1102 70-71 0.2740393086866284 1102 72-73 0.14496998873235256 1102 74-75 -0.3609359050986747 1102 76-77 0.12249212964846379 1102 78-79 0.27093732449334595 1102 80-81 0.10275685379323463 1102 82-83 0.2313166721331008 1102 84-85 0.34890330019913307 1102 86-87 0.29850046205457303 1102 88-89 0.1845007752360246 1102 90-91 0.4280287848174069 1102 92-93 0.564454526097478 1102 94-95 0.24570394002723717 1102 96-97 0.31954744512061595 1102 98-99 0.054631027159476986 1102 100-101 -0.03873183438018657 1103 1 0.4609643206518861 1103 2 0.5272409107822398 1103 3 0.3228028658893294 1103 4 0.17737133283919349 1103 5 0.14178902739844546 1103 6 0.1330398184076529 1103 7 0.1679800478100546 1103 8 0.26085942121925854 1103 9 0.05990040650683426 1103 10-11 0.16902603336605182 1103 12-13 0.2996638598892005 1103 14-15 0.3353166630841571 1103 16-17 0.26798469025158766 1103 18-19 0.40705741253415084 1103 20-21 0.3475365109144164 1103 22-23 0.18168047503788642 1103 24-25 0.25711426914110547 1103 26-27 0.30489257426252436 1103 28-29 0.4506548387854963 1103 30-31 0.3500799269919881 1103 32-33 0.2229598491442104 1103 34-35 0.2741684903487993 1103 36-37 0.38304544518216943 1103 38-39 0.4013443511961867 1103 40-41 0.3925012254290152 1103 42-43 0.36676403124378965 1103 44-45 0.551448906412574 1103 46-47 0.3966717277665808 1103 48-49 0.2939087762039563 1103 50-51 0.11691064694724673 1103 52-53 0.3521804647846878 1103 54-55 0.2579146711342375 1103 56-57 0.2927901402040902 1103 58-59 0.3006213487143725 1103 60-61 0.3144441335569752 1103 62-63 0.02418946826778523 1103 64-65 0.20473889958390856 1103 66-67 0.17867869154379434 1103 68-69 0.16894212056070046 1103 70-71 0.2544791306640626 1103 72-73 0.09479574372956634 1103 74-75 0.15524573774225914 1103 76-77 0.20620805421062727 1103 78-79 0.3589529685100956 1103 80-81 0.33231191340913924 1103 82-83 0.4597613469273334 1103 84-85 0.6828184171618119 1103 86-87 0.5651249695529721 1103 88-89 0.4732427301837312 1103 90-91 0.1920048069516156 1103 92-93 0.2795929551463061 1103 94-95 0.28273632136271587 1103 96-97 0.1197548888568214 1103 98-99 0.16536975377209373 1103 100-101 0.43732317624340666 1104 1 0.3849462434252473 1104 2 0.5149301322421778 1104 3 0.36208718884078195 1104 4 0.19888578753734976 1104 5 0.24090704795983697 1104 6 0.2084602543253311 1104 7 0.20902124648246456 1104 8 0.1263553433450184 1104 9 0.057017182456483795 1104 10-11 0.046036195165577 1104 12-13 0.12446831418260018 1104 14-15 0.17794822006308664 1104 16-17 0.07562355805965382 1104 18-19 0.1253558613229302 1104 20-21 0.09959726668719071 1104 22-23 0.14194017390256164 1104 24-25 0.2124078533193554 1104 26-27 0.22899149559901844 1104 28-29 0.17484337469709743 1104 30-31 0.18381959317294871 1104 32-33 0.18821317316824349 1104 34-35 0.14422261325167796 1104 36-37 0.25849162078372245 1104 38-39 0.24414761147182418 1104 40-41 0.016580731328268428 1104 42-43 0.07218283550582782 1104 44-45 0.1221571461470603 1104 46-47 0.25824819802744514 1104 48-49 0.4189995291434627 1104 50-51 0.36238691331439554 1104 52-53 0.20810780172030974 1104 54-55 0.39240736536449816 1104 56-57 0.46012243383127327 1104 58-59 0.4006864252124629 1104 60-61 0.3652066479762581 1104 62-63 0.302342484680473 1104 64-65 0.30183252879079703 1104 66-67 0.41140401180364705 1104 68-69 0.47827651889716805 1104 70-71 0.24927743778971845 1104 72-73 0.4066402355637031 1104 74-75 0.2896043579434213 1104 76-77 0.5318779816588695 1104 78-79 0.437037493331502 1104 80-81 0.46252530613979204 1104 82-83 0.5238778243725406 1104 84-85 0.7091914743532399 1104 86-87 0.59699095117724 1104 88-89 0.6487310885104876 1104 90-91 0.6327817153562876 1104 92-93 0.7675841593840573 1104 94-95 0.9131378604313802 1104 96-97 0.27752903230085124 1104 98-99 0.5770219252654272 1104 100-101 0.4632886357378716 1105 1 0.3470220129595809 1105 2 0.3717616678029927 1105 3 0.11174517358163882 1105 4 0.0013105880284527416 1105 5 -0.13686634500692918 1105 6 0.057337742705577455 1105 7 0.1115468976269014 1105 8 0.02038323074307158 1105 9 0.10656554692197773 1105 10-11 0.060444684159705275 1105 12-13 0.03783541056075279 1105 14-15 0.20958024672273723 1105 16-17 0.0412027915946922 1105 18-19 0.07182389605063122 1105 20-21 0.1500357477886567 1105 22-23 0.17745275831017437 1105 24-25 0.051257278909119464 1105 26-27 0.053702098072051285 1105 28-29 0.08850206100771629 1105 30-31 0.10837891966598079 1105 32-33 0.0521875658719253 1105 34-35 -0.024377755072450213 1105 36-37 0.18829956606128206 1105 38-39 -0.1849346475828142 1105 40-41 -0.04356791376512348 1105 42-43 0.09022633283109371 1105 44-45 -0.06241708870342677 1105 46-47 0.1569853724552246 1105 48-49 -0.011902426482244266 1105 50-51 -0.058539597253002285 1105 52-53 -0.12900085389663474 1105 54-55 -0.2975645718450082 1105 56-57 -0.3470335777446323 1105 58-59 -0.1594709895029709 1105 60-61 -0.18101145252361306 1105 62-63 -0.33579221671390513 1105 64-65 -0.13865243130742044 1105 66-67 -0.04480268818758759 1105 68-69 -0.32207207357849654 1105 70-71 -0.3383398559049233 1105 72-73 -0.19875965920083516 1105 74-75 -0.28787086787435 1105 76-77 -0.1443146869371148 1105 78-79 -0.24726269083056707 1105 80-81 -0.36537963115740624 1105 82-83 -0.12309884843286412 1105 84-85 0.008799033767431297 1105 86-87 -0.22702246634446155 1105 88-89 -0.006618380927381651 1105 90-91 -0.41688942381761507 1105 92-93 -0.05961263093428215 1105 94-95 -0.009823202446806789 1105 96-97 -0.11851586816393578 1105 98-99 -0.022839953187610007 1105 100-101 0.06793215060238111 1106 1 0.4239296434816673 1106 2 0.4258159802910413 1106 3 0.08744798482661764 1106 4 0.07533047710857943 1106 5 0.03277412162149318 1106 6 0.04918130744071192 1106 7 0.07683662797687418 1106 8 0.01326905977347792 1106 9 0.07439878463473093 1106 10-11 0.07993215786404306 1106 12-13 0.08729515429759971 1106 14-15 0.05618571621977253 1106 16-17 0.11180902605634202 1106 18-19 0.09077111349182587 1106 20-21 0.05262706544021256 1106 22-23 0.08576218649911738 1106 24-25 0.04062427068574692 1106 26-27 0.044250003981396446 1106 28-29 0.25001477854453213 1106 30-31 0.111333595512221 1106 32-33 0.17058496499221576 1106 34-35 -0.003408162416114635 1106 36-37 0.18061167162640146 1106 38-39 0.307410925184449 1106 40-41 0.33375566626929754 1106 42-43 0.45108883044058246 1106 44-45 0.3447162063816762 1106 46-47 0.17609036384936871 1106 48-49 0.19357185158239076 1106 50-51 0.21120127098184582 1106 52-53 0.45123437272413724 1106 54-55 0.3721523912454501 1106 56-57 0.18268529775738074 1106 58-59 0.3648214182231797 1106 60-61 0.40265177180226175 1106 62-63 0.025108529126605106 1106 64-65 0.17334795117488966 1106 66-67 0.3574109281826523 1106 68-69 0.3012067560256355 1106 70-71 0.3440277095951707 1106 72-73 0.25320534366778347 1106 74-75 0.5446688146653322 1106 76-77 0.519191719641583 1106 78-79 0.49586697067259067 1106 80-81 0.30050294677336 1106 82-83 0.3466898300907566 1106 84-85 0.7150000282214393 1106 86-87 0.5098249232137704 1106 88-89 0.3894324892198746 1106 90-91 0.29435555610206166 1106 92-93 0.6541710753193044 1106 94-95 0.8327614217643209 1106 96-97 0.8926908635247237 1106 98-99 0.5267440973000532 1106 100-101 0.5596992188754761 1107 1 0.5125983369471179 1107 2 0.5604354550749093 1107 3 0.4218697841735377 1107 4 0.31041161161545716 1107 5 0.04860316990183833 1107 6 0.2437715062421475 1107 7 0.18045358802891798 1107 8 0.13443159152040352 1107 9 0.22064357688785918 1107 10-11 0.29251707697976315 1107 12-13 0.2732789649295384 1107 14-15 0.3257499544812319 1107 16-17 0.3591823198545896 1107 18-19 0.4280729688884861 1107 20-21 0.31283800264699124 1107 22-23 0.4275417658758158 1107 24-25 0.20015210163571595 1107 26-27 0.1797627715626291 1107 28-29 0.2858540859298415 1107 30-31 0.3373879687684891 1107 32-33 0.47203625301033014 1107 34-35 0.2457379547628875 1107 36-37 0.37748514683565304 1107 38-39 0.42369537763599396 1107 40-41 0.5076299797224877 1107 42-43 0.2691686262593649 1107 44-45 0.22038961653318978 1107 46-47 0.6357570680493438 1107 48-49 0.5199214868837174 1107 50-51 0.36947924268172017 1107 52-53 0.06775778725452852 1107 54-55 0.19609363910291933 1107 56-57 0.250058841777701 1107 58-59 0.1524003945646797 1107 60-61 0.23163874774341053 1107 62-63 0.23312189098525238 1107 64-65 0.3898225724012221 1107 66-67 0.35371014373645693 1107 68-69 0.4006137297147845 1107 70-71 0.45786248796096984 1107 72-73 0.5256321224339366 1107 74-75 0.5228619610827785 1107 76-77 0.49399936142366485 1107 78-79 0.4840865155186407 1107 80-81 0.46761250650762776 1107 82-83 0.31902027335915406 1107 84-85 0.4733480621015076 1107 86-87 0.9061291432090144 1107 88-89 0.9637346824163373 1107 90-91 0.8254781774284901 1107 92-93 0.8914591685020028 1107 94-95 0.8748637876140144 1107 96-97 0.9255725460179356 1107 98-99 1.1768448116840666 1107 100-101 1.2795105514331198 1108 1 0.312320520422265 1108 2 0.3486865671159549 1108 3 0.08369790990004589 1108 4 0.04173939636770285 1108 5 0.09472865617762949 1108 6 0.002729829888714619 1108 7 0.04691470146485699 1108 8 -0.0046878424623315595 1108 9 -0.04865537229147776 1108 10-11 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-1.4401533879462889 2316 58-59 -1.4091334760871632 2316 60-61 -1.367645073943713 2316 62-63 -1.343579032507833 2316 64-65 -1.4748680444803313 2316 66-67 -1.2849385408679233 2316 68-69 -1.4573881217059323 2316 70-71 -1.3792665349988624 2316 72-73 -1.3090375781976142 2316 74-75 -1.542371517481758 2316 76-77 -1.2985611423401515 2316 78-79 -1.4656960072176162 2316 80-81 -1.1330404796011742 2316 82-83 -0.8870597590129883 2316 84-85 -1.2941397336731129 2316 86-87 -1.2970402599387683 2316 88-89 -1.4100980171472308 2316 90-91 -1.4178977274949602 2316 92-93 -1.689306750575021 2316 94-95 -1.9724905469646856 2316 96-97 -2.0227849186073676 2316 98-99 -2.413523735597373 2316 100-101 -3.037198335755864 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 14.0 5 34.0 6 61.0 7 197.0 8 440.0 9 856.0 10 1483.0 11 2406.0 12 3514.0 13 4791.0 14 6079.0 15 8123.0 16 10472.0 17 13531.0 18 11825.0 19 7476.0 20 6622.0 21 6860.0 22 7170.0 23 8034.0 24 9254.0 25 11319.0 26 13639.0 27 17073.0 28 21227.0 29 26159.0 30 32461.0 31 40089.0 32 50023.0 33 63461.0 34 83939.0 35 114007.0 36 160995.0 37 211229.0 38 135596.0 39 8503.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.72130476194858 19.13025072816539 15.228296530113608 21.920147979772416 2 15.519373735590394 21.334816118554865 39.1011190963504 24.044691049504337 3 20.5533639999454 24.63602735425397 30.35930895474172 24.45129969105891 4 12.942180786631074 17.215620497876294 37.61602567194552 32.226173043547114 5 13.318222186633278 37.62384346041013 33.97787742682225 15.080056926134336 6 30.763862096075588 36.55383960224501 17.326346172045046 15.355952129634348 7 27.959685814327624 33.2026046254304 20.76320693596174 18.074502624280235 8 26.46200799295339 33.10769740338208 19.7618129008306 20.668481702833933 9 25.834094689676007 17.505250628801473 21.01015910224257 35.65049557927995 10-11 25.013967725908632 26.291263938152547 27.102938955123108 21.591829380815717 12-13 26.973752157140368 23.84085411156755 27.586691678044783 21.5987020532473 14-15 23.03902388422593 25.37116640338936 27.443019268986905 24.146790443397805 16-17 21.4693694447882 28.611024160849084 27.804949734660156 22.114656659702558 18-19 21.127548754831807 27.348567019239866 30.5045278843424 21.019356341585922 20-21 22.197052143465505 25.53240858696523 30.4926076100487 21.77793165952057 22-23 21.645654280657535 25.672616258172535 30.65698777474965 22.02474168642028 24-25 22.276990776801508 25.83109790275968 30.709993375603297 21.18191794483552 26-27 21.942368632375388 25.53938128288598 31.28181679622009 21.23643328851854 28-29 21.040876531436766 26.794183270122513 30.95881772717677 21.20612247126395 30-31 21.49704154126487 25.856451623910626 30.69058002170219 21.955926813122318 32-33 21.529386981251317 26.001325834527293 30.76841529104889 21.7008718931725 34-35 22.606368942216175 25.60293478003709 30.37045091805146 21.420245359695276 36-37 22.398190251124493 25.280066100240507 30.99868327301747 21.323060375617526 38-39 21.83402398254359 26.062953721953182 30.870654779128742 21.232367516374484 40-41 21.804528040912523 26.149299773415958 30.610730437789485 21.43544174788203 42-43 21.60033780601726 26.42221838995209 29.756733255646445 22.220710548384208 44-45 22.00491923214117 25.97784070641848 29.542054612030373 22.475185449409985 46-47 22.606389876076204 26.597594139451463 29.130986499281352 21.665029485190974 48-49 22.62474837375734 26.570821184290516 28.938155456785807 21.86627498516634 50-51 21.83655613989351 26.88549084160589 29.401950654456993 21.876002364043607 52-53 22.43290856346251 27.445793613528576 28.148238133196475 21.973059689812438 54-55 21.80712744189348 26.715826459198144 28.79014765683334 22.686898442075034 56-57 22.61549115190407 26.792573186633994 28.394939837315512 22.196995824146427 58-59 23.234849043229172 26.506405619146157 28.15392230997209 22.104823027652586 60-61 22.402265164989316 26.668741460245183 29.507796173370842 21.421197201394662 62-63 22.09293014223797 26.86569608884254 29.16770176161786 21.87367200730163 64-65 21.77953040397417 27.508030384179765 28.650281148766993 22.06215806307907 66-67 22.0164940460916 27.049698710214003 28.242800802954115 22.691006440740274 68-69 21.865533723942576 26.94275624290811 28.557181425829892 22.634528607319417 70-71 22.50763905444976 26.96754562048777 28.28192706181674 22.24288826324573 72-73 22.27194058425723 26.528297457250805 28.95229888404394 22.247463074448028 74-75 21.830708446469778 27.905589607704684 27.999177410079273 22.264524535746265 76-77 22.123381252903314 27.03602817028289 28.03215223126967 22.808438345544126 78-79 22.08219275319059 27.170257067350047 28.224626338664937 22.522923840794427 80-81 22.49388664052354 27.290155011316653 27.95504826528021 22.26091008287959 82-83 22.24205611601599 27.072066804477597 28.471304278979314 22.214572800527097 84-85 21.914346450788614 27.43490716103129 28.14334802692166 22.50739836125843 86-87 22.1158956307764 27.358290531710814 27.822177003971362 22.703636833541427 88-89 21.922666466426836 27.338744359721755 27.454155317846602 23.284433856004803 90-91 22.088071413879035 26.783481171761377 28.00348950872013 23.124957905639462 92-93 22.441644190625944 26.90541317696561 28.088226194506017 22.56471643790243 94-95 21.918424659154816 26.884317390520945 28.16274845616379 23.03450949416045 96-97 21.322400915483826 26.855617917456303 28.88046611413062 22.941515052929255 98-99 21.654323639097033 27.15936247003729 27.688953885187097 23.49736000567858 100-101 21.05065345053369 26.888663604702696 28.20580256429624 23.854880380467375 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 272.0 1 216.5 2 285.0 3 376.0 4 289.0 5 258.5 6 379.5 7 720.5 8 1190.5 9 1497.0 10 1453.0 11 1336.0 12 1427.0 13 1579.5 14 1706.5 15 1880.5 16 2035.5 17 2148.5 18 2272.5 19 2419.5 20 2623.5 21 2847.5 22 3202.5 23 3760.5 24 4324.0 25 5094.5 26 6333.5 27 7957.5 28 9400.5 29 10916.0 30 13093.5 31 15519.0 32 17917.0 33 19796.0 34 21953.5 35 23900.5 36 25850.0 37 27830.5 38 28816.5 39 29365.0 40 30249.5 41 32579.5 42 34722.0 43 36003.5 44 37463.0 45 38932.5 46 39702.5 47 45625.5 48 59664.5 49 57476.0 50 42843.0 51 36852.5 52 34360.5 53 33554.0 54 32463.0 55 30166.0 56 27147.0 57 24277.5 58 22448.5 59 20028.0 60 16966.5 61 13883.5 62 11148.0 63 8733.0 64 6388.5 65 4839.0 66 3803.0 67 3103.0 68 2314.5 69 1574.0 70 1207.5 71 946.5 72 781.5 73 655.5 74 554.5 75 473.5 76 336.5 77 198.0 78 115.0 79 89.0 80 65.5 81 41.5 82 34.5 83 23.5 84 8.5 85 3.5 86 4.0 87 4.0 88 2.0 89 2.0 90 1.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.122234678352782 2 0.012284252134730039 3 0.004822706393634755 4 0.016469997306563976 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0047317119333774955 10-11 5.459667615435572E-4 12-13 1.364916903858893E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0011829279833443739 24-25 0.0 26-27 4.549723012862977E-5 28-29 0.0 30-31 4.549723012862977E-5 32-33 0.0034122922596472325 34-35 9.099446025725953E-4 36-37 0.0028208282679750454 38-39 1.8198892051451908E-4 40-41 0.0034577894897758624 42-43 0.010236876778941698 44-45 0.0014559113641161526 46-47 0.0024113531968173777 48-49 0.010555357389842106 50-51 3.184806109004084E-4 52-53 0.0018198892051451906 54-55 0.009326932176369103 56-57 0.0028208282679750454 58-59 0.001000939062829855 60-61 0.005778148226335981 62-63 0.003184806109004084 64-65 0.01569654439437727 66-67 0.0023658559666887478 68-69 9.099446025725954E-5 70-71 9.099446025725953E-4 72-73 0.0 74-75 0.0 76-77 7.734529121867062E-4 78-79 0.010191379548813068 80-81 0.013194196737302634 82-83 0.010873838000742514 84-85 0.006915578979551725 86-87 0.009099446025725953 88-89 0.0259789184034476 90-91 0.022384637223285847 92-93 0.0018198892051451906 94-95 0.007461545741095282 96-97 0.008689970954568287 98-99 0.009281434946240473 100-101 0.009918396168041289 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1098968.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.2572976779583 #Duplication Level Percentage of deduplicated Percentage of total 1 83.84210308552231 50.52098563570384 2 9.633360257060042 11.609605132973597 3 2.573235668606966 4.6516868303637 4 1.148638946183269 2.7685551561864625 5 0.6208894356845253 1.8706559775570994 6 0.4268679514193491 1.5433145530713575 7 0.29159113950566107 1.229934586541338 8 0.21109469371781692 1.0175996638073541 9 0.1617474120892131 0.8771815763009194 >10 0.970283498211661 11.056955320004622 >50 0.07562494885376835 3.118373816905757 >100 0.03954392807463355 4.3013383948531985 >500 0.0022813795775737523 0.9381853455434287 >1k 0.0022813795775737523 2.812539813361559 >5k 4.562759155147504E-4 1.6830881968257365 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7857 0.7149434742412881 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5507 0.5011064926367282 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5001 0.4550632957465549 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4273 0.38881932867927 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3053 0.2778060871654134 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2959 0.269252607901231 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2875 0.2616090732396212 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2288 0.20819532506860983 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2216 0.20164372393008714 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2033 0.18499173770300864 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1590 0.14468119180904268 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1498 0.13630970146537477 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1412 0.12848417788325048 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1397 0.12711926097939155 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1300 0.11829279833443741 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1283 0.11674589251006398 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1277 0.11619992574852044 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1235 0.11237815841771553 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 9.099446025725954E-5 0.0 0.0 0.0 0.0 8 9.099446025725954E-5 0.0 0.0 0.0 0.0 9 9.099446025725954E-5 0.0 0.0 0.0 0.0 10-11 9.099446025725954E-5 0.0 0.0 0.0 0.0 12-13 9.099446025725954E-5 0.0 0.0 0.0 0.0 14-15 9.099446025725954E-5 0.0 0.0 0.0 0.0 16-17 9.099446025725954E-5 0.0 0.0 0.0 0.0 18-19 9.099446025725954E-5 0.0 0.0 0.0 0.0 20-21 9.099446025725954E-5 0.0 0.0 0.0 0.0 22-23 9.099446025725954E-5 0.0 0.0 0.0 4.549723012862977E-5 24-25 9.099446025725954E-5 0.0 0.0 0.0 9.099446025725954E-5 26-27 9.099446025725954E-5 0.0 0.0 2.729833807717786E-4 9.099446025725954E-5 28-29 9.099446025725954E-5 0.0 0.0 6.824584519294465E-4 9.099446025725954E-5 30-31 9.099446025725954E-5 0.0 0.0 0.0017288947448879312 9.099446025725954E-5 32-33 9.099446025725954E-5 0.0 0.0 0.003093811648746824 9.099446025725954E-5 34-35 1.364916903858893E-4 0.0 0.0 0.004413231322477088 9.099446025725954E-5 36-37 1.8198892051451908E-4 0.0 0.0 0.006051131607107759 9.099446025725954E-5 38-39 4.549723012862977E-4 0.0 0.0 0.009599915557140883 9.099446025725954E-5 40-41 4.549723012862977E-4 0.0 0.0 0.012011268753958259 9.099446025725954E-5 42-43 5.004695314149275E-4 0.0 0.0 0.018790356043124096 9.099446025725954E-5 44-45 7.279556820580763E-4 0.0 0.0 0.026479387934862528 9.099446025725954E-5 46-47 8.189501423153359E-4 0.0 0.0 0.03353145860480014 9.099446025725954E-5 48-49 0.001000939062829855 0.0 0.0 0.043677340923484576 9.099446025725954E-5 50-51 0.001000939062829855 0.0 0.0 0.05559761521718558 9.099446025725954E-5 52-53 0.001000939062829855 0.0 0.0 0.07766377182957102 9.099446025725954E-5 54-55 0.001000939062829855 0.0 0.0 0.09249586885150432 9.099446025725954E-5 56-57 0.001000939062829855 0.0 0.0 0.1289846474146654 9.099446025725954E-5 58-59 0.001000939062829855 0.0 0.0 0.17780317534268514 9.099446025725954E-5 60-61 0.001000939062829855 0.0 0.0 0.2200701021321822 9.099446025725954E-5 62-63 0.001000939062829855 0.0 0.0 0.29218321188606033 9.099446025725954E-5 64-65 0.001000939062829855 0.0 0.0 0.3416387010358809 9.099446025725954E-5 66-67 0.0010919335230871144 0.0 0.0 0.44159611562848056 9.099446025725954E-5 68-69 0.0010919335230871144 0.0 0.0 0.5602073945738184 9.099446025725954E-5 70-71 0.0012284252134730036 0.0 0.0 0.6381441497841611 9.099446025725954E-5 72-73 0.001364916903858893 0.0 0.0 0.7733164204963201 9.099446025725954E-5 74-75 0.001364916903858893 0.0 0.0 0.9096716191918237 9.099446025725954E-5 76-77 0.001364916903858893 0.0 0.0 0.9961618536663488 9.099446025725954E-5 78-79 0.001364916903858893 0.0 0.0 1.090887086794156 9.099446025725954E-5 80-81 0.0016833975147593015 0.0 0.0 1.2640950418938495 9.099446025725954E-5 82-83 0.0020928725859169694 0.0 0.0 1.404363002380415 9.099446025725954E-5 84-85 0.002183867046174229 0.0 0.0 1.6250245685042695 1.8198892051451908E-4 86-87 0.0022748615064314883 0.0 0.0 1.9705760313312126 1.8198892051451908E-4 88-89 0.0022748615064314883 0.0 0.0 2.4262307910694396 1.8198892051451908E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 970 0.0 33.297787 2 GTACCTG 1125 0.0 31.168646 1 CGTATAG 65 0.0055332957 29.559303 1 GTACATG 4660 0.0 22.676935 1 ATATCGT 110 0.002879399 21.588179 6 ATCGCTC 110 0.002879399 21.588179 8 GAGTACT 6030 0.0 21.381193 12-13 ACATGGG 4665 0.0 21.07834 3 TACATGG 4830 0.0 20.848124 2 AGTACTT 6250 0.0 20.286663 12-13 ACCTGGG 1620 0.0 19.93938 3 GTACTTT 6435 0.0 19.777237 14-15 GTGTAGC 495 0.0 19.407625 1 TATACAG 510 0.0 18.625095 5 GTGTATC 240 2.7771293E-6 18.012703 1 CATGGGA 2655 0.0 17.712042 4 ACGTCTA 295 6.346636E-8 17.709625 5 TACTTTT 7220 0.0 17.56117 14-15 CATGGGG 2075 0.0 17.397764 4 AGAGTAC 10020 0.0 17.250952 10-11 >>END_MODULE