FastQCFastQC Report
Thu 26 May 2016
SRR936493_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936493_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1127551
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT79190.7023185647478473No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT45690.40521448697220785No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43370.38463892098893976No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35540.3151963857954097No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT28680.2543565656897116No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC28670.254267877905301No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT22830.20247421180948802No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20200.17914932450949003No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC19090.16930498043990916No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT17860.1583963829574006No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC16880.149704980085158No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG15590.13826425589618563No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA13690.12141357685816428No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC13130.11644706093116852No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA12660.11227873506386851No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA12360.10961810153154936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG9800.031.0378842
GTACCTG10850.028.9090061
GTACATG51650.024.659451
TACATGG51850.023.5571422
GTGTAAG3800.022.5116771
ACATGGG52700.022.4557193
GTGTAGC4050.022.2955151
GAGTACT40850.021.4674812-13
GTACTTT44450.019.621914-15
ATTCGCG1603.965397E-1019.29575780-81
CATGGGA28500.019.1783164
CGCGGAA1756.366463E-1118.99721382-83
AGTACTT42700.018.7563712-13
GTATTAG2803.4673576E-818.6703991
TAAGGTG4350.018.572035
CATGGGG25400.018.5250344
TCGCGCT1708.949428E-1018.15869960-61
CGCTCCG1851.4188117E-1017.96874648-49
ACGTAAC1350.00937224617.6017231
ATTAGAC3007.630297E-817.426483
Produced by FastQC (version 0.11.5)