FastQCFastQC Report
Thu 26 May 2016
SRR936493_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936493_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1127551
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT83280.7385918685717985No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57650.5112850771273317No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51200.45408145618246976No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT43530.38605792553950996No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31550.2798099598155649No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC30290.2686352989798244No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT29010.25728326257526263No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT22500.1995475149239369No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC21880.19404887229047735No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT21560.1912108631893369No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC16170.14340814739200267No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15310.13578099793268775No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG14920.13232217434067284No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA14260.12646878056957067No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA13640.1209701379361111No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA13330.1182208166193813No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC13060.11582624644029406No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA11500.10199095207223442No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA651.3287966E-436.5355264
TATCACG753.076659E-431.669742
TACCTGG11400.027.9193782
ATAACGC1056.905325E-527.1430853
GTACCTG12400.026.829021
GAGTACT63200.022.13330312-13
GTACATG46600.021.3151721
AGTACTT65450.020.97327212-13
TACATGG48300.020.7525332
GTACTTT68250.020.495614-15
CGTACAC1404.8513847E-420.3573153
ACATGGG47150.020.1486323
TAAGGTG4450.019.2136445
TATAACG1250.0060276919.0018462
GTACACG1559.5894746E-418.3970411
TACTTTT76350.018.10347414-15
ACTTTTT78400.018.05299816-17
GTGTAGC5600.017.8221341
AGAGTAC105500.017.51514210-11
CATGGGA26300.017.3370114
Produced by FastQC (version 0.11.5)