##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936493_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1127551 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.889043599801695 31.0 31.0 34.0 30.0 34.0 2 32.03189035351838 31.0 31.0 34.0 30.0 34.0 3 32.13653839161155 33.0 31.0 34.0 30.0 34.0 4 35.87888796160883 37.0 35.0 37.0 35.0 37.0 5 35.65950276306792 37.0 35.0 37.0 33.0 37.0 6 35.76891156142826 37.0 35.0 37.0 33.0 37.0 7 35.72097581395431 37.0 35.0 37.0 33.0 37.0 8 35.701354528531304 37.0 35.0 37.0 33.0 37.0 9 37.33757674819143 39.0 37.0 39.0 34.0 39.0 10-11 37.20322983173267 39.0 37.0 39.0 33.0 39.0 12-13 37.11194615587233 39.0 37.0 39.0 33.0 39.0 14-15 38.195680284084716 40.0 37.5 41.0 33.0 41.0 16-17 38.13591757712068 40.0 37.0 41.0 33.0 41.0 18-19 38.31363326359517 40.0 38.0 41.0 33.5 41.0 20-21 38.3500347212676 40.0 38.0 41.0 34.0 41.0 22-23 38.261708783017355 40.0 38.0 41.0 34.0 41.0 24-25 38.13083576707395 40.0 37.0 41.0 33.0 41.0 26-27 37.905481880642206 40.0 37.0 41.0 33.0 41.0 28-29 37.820806331598305 40.0 37.0 41.0 33.0 41.0 30-31 37.772853290006395 40.0 37.0 41.0 33.0 41.0 32-33 37.6105701648972 40.0 36.5 41.0 33.0 41.0 34-35 37.40443093039694 39.5 36.0 41.0 32.0 41.0 36-37 37.132282264837684 39.0 36.0 41.0 31.5 41.0 38-39 36.9549164516727 39.0 36.0 41.0 31.0 41.0 40-41 36.75040463801638 39.0 35.0 41.0 31.0 41.0 42-43 36.48751142963822 39.0 35.0 40.5 30.0 41.0 44-45 36.202161143930525 39.0 35.0 40.0 30.0 41.0 46-47 35.800574874218555 39.0 35.0 40.0 28.5 41.0 48-49 35.39120669486347 38.0 35.0 40.0 27.0 41.0 50-51 35.05693223632457 38.0 34.5 40.0 26.5 41.0 52-53 34.79018598715268 38.0 34.0 40.0 26.0 41.0 54-55 34.53129880599636 38.0 34.0 40.0 25.0 41.0 56-57 34.21435793148159 38.0 33.5 40.0 24.0 41.0 58-59 33.87901921952976 37.5 33.0 40.0 23.0 41.0 60-61 33.49529245240348 37.0 33.0 40.0 22.0 41.0 62-63 33.11690247270412 36.0 32.0 39.0 22.0 40.5 64-65 32.70579157838537 36.0 31.5 39.0 20.5 40.0 66-67 32.228066845756864 35.0 31.0 38.5 19.5 40.0 68-69 31.950365881454587 35.0 31.0 38.0 18.5 40.0 70-71 31.50020353846522 35.0 31.0 37.5 17.5 39.5 72-73 31.0433763084774 34.5 30.0 37.0 14.0 39.0 74-75 30.526769964285428 34.0 30.0 36.5 9.5 39.0 76-77 29.100970155673668 32.5 28.0 35.0 8.0 37.0 78-79 29.875580794128158 34.0 29.0 35.0 7.0 37.5 80-81 29.65580714309153 34.0 29.0 35.0 6.5 37.0 82-83 29.319216603062742 34.0 29.0 35.0 2.0 36.5 84-85 28.898209925759456 34.0 29.0 35.0 2.0 36.0 86-87 28.5280985072959 34.0 29.0 35.0 2.0 36.0 88-89 28.112988680778074 33.0 27.5 35.0 2.0 35.0 90-91 27.608560943141377 33.0 26.5 35.0 2.0 35.0 92-93 27.13652863595527 33.0 26.0 34.5 2.0 35.0 94-95 26.547757928466204 32.0 25.0 34.0 2.0 35.0 96-97 25.77066004109792 32.0 23.5 34.0 2.0 35.0 98-99 24.830698567071465 32.0 18.5 34.0 2.0 35.0 100-101 21.61084953141809 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3135800129243016 1101 2 0.4515340526723719 1101 3 0.28829197631734615 1101 4 0.21408533745748315 1101 5 0.19436867519665668 1101 6 0.20554027680467613 1101 7 0.1881515083212335 1101 8 0.23764137745116187 1101 9 0.32436898843189255 1101 10-11 0.36322658357973125 1101 12-13 0.2889267038740684 1101 14-15 0.42159987973581536 1101 16-17 0.518505068476145 1101 18-19 0.5036850534574384 1101 20-21 0.3873499696588709 1101 22-23 0.3354781864465224 1101 24-25 0.16628817773555937 1101 26-27 0.26829118679238917 1101 28-29 0.354633014877173 1101 30-31 0.36107304520844963 1101 32-33 0.4007884954588832 1101 34-35 0.4442466716225155 1101 36-37 0.30634876525151356 1101 38-39 0.35385124471972773 1101 40-41 0.1702203685794217 1101 42-43 0.4175018067390823 1101 44-45 0.4845123104512865 1101 46-47 0.3403952930847396 1101 48-49 0.4829655606083847 1101 50-51 0.368525319035399 1101 52-53 0.46426119816300115 1101 54-55 0.47207521597803037 1101 56-57 0.31365319734279495 1101 58-59 0.3919216777697443 1101 60-61 0.3672758714126587 1101 62-63 0.2783362506605229 1101 64-65 0.32733243170481785 1101 66-67 0.18496219402772596 1101 68-69 0.14623107286086778 1101 70-71 0.42296363996832653 1101 72-73 0.4539090893150437 1101 74-75 0.3739748771292817 1101 76-77 0.1934903658389473 1101 78-79 0.16415642203622482 1101 80-81 0.5065289035816036 1101 82-83 0.7229142575516043 1101 84-85 0.5884756245406635 1101 86-87 0.6798638784657989 1101 88-89 0.5524578812650418 1101 90-91 0.5879761369713847 1101 92-93 0.6089327513572051 1101 94-95 0.6057554749996825 1101 96-97 0.7139541446344211 1101 98-99 0.833027392107752 1101 100-101 1.3285124450410457 1102 1 0.27699896205340124 1102 2 0.29176374433426844 1102 3 0.2822521470953063 1102 4 0.17916703350883267 1102 5 0.06421852073687262 1102 6 0.29577646357174103 1102 7 0.21068024452223 1102 8 0.27763019349865203 1102 9 0.35807487274973937 1102 10-11 0.32772310158478035 1102 12-13 0.3856688209814365 1102 14-15 0.4719783897215706 1102 16-17 0.41768804610453003 1102 18-19 0.3452128382801689 1102 20-21 0.281431196825487 1102 22-23 0.2088217799840777 1102 24-25 0.2606301988390811 1102 26-27 0.09230538447369696 1102 28-29 0.1318518355887619 1102 30-31 0.32994509905967817 1102 32-33 0.34978088814428077 1102 34-35 0.3460125360715338 1102 36-37 0.46022832249565937 1102 38-39 0.2760469985292602 1102 40-41 0.3205962805231195 1102 42-43 0.459453072418583 1102 44-45 0.5121081565531753 1102 46-47 0.6204280407864005 1102 48-49 0.5904063530854486 1102 50-51 0.31228389412067514 1102 52-53 0.3974544021798181 1102 54-55 0.5229717879233888 1102 56-57 0.49711022634560464 1102 58-59 0.18633458830679217 1102 60-61 0.33492189684130835 1102 62-63 0.16181128038335402 1102 64-65 0.5105166662906981 1102 66-67 0.5711413525342763 1102 68-69 0.35334327487471384 1102 70-71 0.38825750945506243 1102 72-73 0.3977010677983088 1102 74-75 0.2807011461800393 1102 76-77 -0.03034240190475046 1102 78-79 0.11641894552685628 1102 80-81 0.4766427596822389 1102 82-83 0.44111545982650213 1102 84-85 0.4855566624213523 1102 86-87 0.4482100762312342 1102 88-89 0.368872805903262 1102 90-91 0.658512360482181 1102 92-93 0.6217083139261028 1102 94-95 0.6256345175840643 1102 96-97 0.38983076732819555 1102 98-99 0.48938591448988333 1102 100-101 1.1755016854354032 1103 1 0.21808228667969232 1103 2 0.18149591560725753 1103 3 0.2791920708816349 1103 4 0.1908410801473721 1103 5 0.06326434862079822 1103 6 0.23420503997576247 1103 7 0.17383683083187407 1103 8 0.11469314483887416 1103 9 0.1411883681902495 1103 10-11 0.14864439109225458 1103 12-13 0.21091390930690324 1103 14-15 0.1691983801378214 1103 16-17 0.2799551926795374 1103 18-19 0.2684626320132608 1103 20-21 0.19361343469236658 1103 22-23 0.23933487117747632 1103 24-25 0.12051912095486017 1103 26-27 0.21244880452920256 1103 28-29 0.20064980690715117 1103 30-31 0.2515591691652048 1103 32-33 0.36090849260180846 1103 34-35 0.2964012906517368 1103 36-37 0.3280649759156091 1103 38-39 0.39519030929633914 1103 40-41 0.37940006454515185 1103 42-43 0.3791095584470696 1103 44-45 0.36822722810587294 1103 46-47 0.3730972006705784 1103 48-49 0.4297409426353127 1103 50-51 0.4335256029114234 1103 52-53 0.3919689633627286 1103 54-55 0.3772761910463913 1103 56-57 0.28705004564738346 1103 58-59 0.3744767329302121 1103 60-61 0.3614048268282488 1103 62-63 0.20776436561992995 1103 64-65 0.17707044160568586 1103 66-67 0.2902484540007819 1103 68-69 0.3493242226576676 1103 70-71 0.2991231819572526 1103 72-73 0.3244237718103946 1103 74-75 0.38427866110511744 1103 76-77 0.16787589634218847 1103 78-79 0.11660779834655699 1103 80-81 0.40222033705785165 1103 82-83 0.5975207229654309 1103 84-85 0.47878318132044484 1103 86-87 0.4826335316913948 1103 88-89 0.3444762642217363 1103 90-91 0.33542812071535977 1103 92-93 0.5369863569184936 1103 94-95 0.4963279827360658 1103 96-97 0.607847086133372 1103 98-99 0.7285573520171305 1103 100-101 0.9815762609819352 1104 1 0.25036949808330533 1104 2 0.2774580663669681 1104 3 0.19295271409080073 1104 4 0.10736645755829954 1104 5 0.0632829546133209 1104 6 0.060561707549474875 1104 7 0.18788377375101106 1104 8 0.13930073282777755 1104 9 0.14584393638237714 1104 10-11 0.2321492484191765 1104 12-13 0.16209149420255642 1104 14-15 0.19596222822918463 1104 16-17 0.3725516938226434 1104 18-19 0.21668796918873312 1104 20-21 0.14727172168515068 1104 22-23 0.21605179728145174 1104 24-25 0.2031759520287224 1104 26-27 0.09054088791196335 1104 28-29 0.09495294271462029 1104 30-31 0.09731316538353241 1104 32-33 0.021502047020845794 1104 34-35 0.10451216608694125 1104 36-37 0.195941497246217 1104 38-39 0.1882657984595113 1104 40-41 0.23543619738263288 1104 42-43 0.29767141988359924 1104 44-45 0.3645226005629567 1104 46-47 0.3532396644295943 1104 48-49 0.1753502191180374 1104 50-51 0.12433761494019535 1104 52-53 0.2003345083206156 1104 54-55 0.1429575541214021 1104 56-57 0.17020586618328082 1104 58-59 0.11723289674667114 1104 60-61 0.10373101778620253 1104 62-63 -0.028427371458789708 1104 64-65 0.15174826721636947 1104 66-67 0.11339500642665001 1104 68-69 0.2788243726684776 1104 70-71 0.2756979466495899 1104 72-73 0.23058880928984138 1104 74-75 0.3262174985561934 1104 76-77 0.14450182751397378 1104 78-79 0.3064537523571964 1104 80-81 0.2720560112576216 1104 82-83 0.37072530283048977 1104 84-85 0.22493610164725908 1104 86-87 0.2478846311892653 1104 88-89 0.13435501129420047 1104 90-91 0.31477546901256304 1104 92-93 0.4753487737438924 1104 94-95 0.481854264863383 1104 96-97 0.813215089408029 1104 98-99 1.064413250930773 1104 100-101 1.5177274137394612 1105 1 0.006146105467227159 1105 2 0.14095479062780214 1105 3 0.13816143964678673 1105 4 0.15854710758819834 1105 5 0.08322984298751379 1105 6 0.12223738717722199 1105 7 -0.04375824762419711 1105 8 0.01701212832660559 1105 9 0.04199099343951218 1105 10-11 -0.011444847155637206 1105 12-13 0.005417094058422833 1105 14-15 0.08751065146147852 1105 16-17 0.2113161957249048 1105 18-19 0.126407344487383 1105 20-21 0.2686907995691499 1105 22-23 0.16415437531036758 1105 24-25 0.21769645901757428 1105 26-27 0.2967241230209936 1105 28-29 0.18985831315123392 1105 30-31 0.22398726524070156 1105 32-33 0.24518254690725882 1105 34-35 0.1537112485144121 1105 36-37 0.09045982389054785 1105 38-39 0.07591506066748366 1105 40-41 -0.0915123386531107 1105 42-43 0.09831514375536443 1105 44-45 0.34919523554194853 1105 46-47 0.3558413899046329 1105 48-49 0.11646492351815141 1105 50-51 0.26921498035288494 1105 52-53 0.12978436953581962 1105 54-55 0.3063782927229042 1105 56-57 0.0530877463452768 1105 58-59 0.22214826804498955 1105 60-61 0.14649514628813876 1105 62-63 0.21526431738124074 1105 64-65 0.004737558721899404 1105 66-67 0.2426032671906384 1105 68-69 0.3180895051733721 1105 70-71 0.03537665264775569 1105 72-73 0.10024827081002385 1105 74-75 0.0018919039227114354 1105 76-77 -0.019573993640332077 1105 78-79 0.13473889180785292 1105 80-81 0.43990173947963385 1105 82-83 0.22965048437354696 1105 84-85 0.016079006910636906 1105 86-87 -0.010100391018916355 1105 88-89 -0.05199818623979269 1105 90-91 -0.010367428417239921 1105 92-93 0.03236608855169365 1105 94-95 0.11999867417280186 1105 96-97 0.4219930650560606 1105 98-99 0.5924434075761233 1105 100-101 0.621413566854482 1106 1 0.06139984049624303 1106 2 0.06631594632603566 1106 3 0.1151339053832956 1106 4 0.10835572593614273 1106 5 -0.016103408031128197 1106 6 -0.08297313264004202 1106 7 -0.10586468051714348 1106 8 -0.0905320129254008 1106 9 -0.0216588398732398 1106 10-11 -0.06728507434418418 1106 12-13 0.04298346027647426 1106 14-15 0.07868240106019897 1106 16-17 0.15961228166143826 1106 18-19 0.059670710335019805 1106 20-21 0.060239138870294084 1106 22-23 0.08053294056533389 1106 24-25 -0.18096386941802933 1106 26-27 0.06731308462118335 1106 28-29 -0.06304327963799494 1106 30-31 0.1861739549554855 1106 32-33 -0.05593487499668015 1106 34-35 0.0645953082748818 1106 36-37 0.07900779277559877 1106 38-39 0.09860304683861187 1106 40-41 0.16598858738714029 1106 42-43 0.11723723290216981 1106 44-45 0.14509596402695024 1106 46-47 -0.252073167448124 1106 48-49 -0.08309675076262124 1106 50-51 -0.15583749526138746 1106 52-53 -0.20362098915044413 1106 54-55 -0.43605904522005545 1106 56-57 -0.3350465912272611 1106 58-59 -0.28174110101292626 1106 60-61 -0.20777698607388118 1106 62-63 -0.1181410413050159 1106 64-65 0.10981225930222394 1106 66-67 0.13842373885380965 1106 68-69 0.120595987455129 1106 70-71 0.1950877280891703 1106 72-73 0.16272511980372784 1106 74-75 0.09770620198419877 1106 76-77 -0.21080130565416155 1106 78-79 -0.08464004386321733 1106 80-81 0.07744958489264775 1106 82-83 0.26508695801227233 1106 84-85 0.0769198514593441 1106 86-87 0.04289997939718404 1106 88-89 0.14478536728533342 1106 90-91 0.04680012313233206 1106 92-93 -0.11663378797627288 1106 94-95 0.3420631265743346 1106 96-97 0.25002739028139587 1106 98-99 0.7892578291096477 1106 100-101 0.9161413110204286 1107 1 -0.023638649036627157 1107 2 0.041459227649092156 1107 3 -0.013179399896301902 1107 4 -0.12598093817580036 1107 5 -0.16325778552258186 1107 6 -0.05774164111641511 1107 7 -0.06908391901728095 1107 8 -0.1435304367103427 1107 9 0.012341854239814154 1107 10-11 -0.04302244895795582 1107 12-13 -0.1353668402648367 1107 14-15 -0.10855339010817744 1107 16-17 0.0894664938190175 1107 18-19 0.08174675909646822 1107 20-21 0.09329024776410222 1107 22-23 -0.03601900463829111 1107 24-25 0.09048735708937272 1107 26-27 0.022631966906359935 1107 28-29 -0.15602008469345208 1107 30-31 -0.23188877069151204 1107 32-33 0.04520739889841252 1107 34-35 -0.06810380335681998 1107 36-37 -0.007843103064061552 1107 38-39 0.09062737453709957 1107 40-41 0.1214400259846471 1107 42-43 -0.22723263825309203 1107 44-45 -0.13466654326364846 1107 46-47 0.17001565492159898 1107 48-49 -0.060614597662493 1107 50-51 -0.008412582387038015 1107 52-53 -0.06217195584747515 1107 54-55 0.06594436268223092 1107 56-57 0.060243562489866065 1107 58-59 0.20151884432567613 1107 60-61 0.2876275275890663 1107 62-63 0.42184276272373467 1107 64-65 0.43527235021175414 1107 66-67 0.36779857918370595 1107 68-69 0.3402918068700522 1107 70-71 -0.006252054711289645 1107 72-73 -0.022072840275132677 1107 74-75 0.10309823125263406 1107 76-77 0.19939962561418767 1107 78-79 0.0373810542814752 1107 80-81 0.4872311561910756 1107 82-83 0.5884622996460358 1107 84-85 0.511062862849549 1107 86-87 0.5736650944319663 1107 88-89 0.5361994539241373 1107 90-91 0.7746216444931804 1107 92-93 0.7148371291688029 1107 94-95 0.8506407250395718 1107 96-97 0.8933707737933183 1107 98-99 0.8642395122373969 1107 100-101 1.1139641338432504 1108 1 -0.14623448902377234 1108 2 -0.08072985106172226 1108 3 -0.07202419787153502 1108 4 -0.02047660441059662 1108 5 -0.15491415761373872 1108 6 -0.021143169735331924 1108 7 -0.1871200153142496 1108 8 -0.07081809225886815 1108 9 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-0.719204076048463 2316 52-53 -0.6872462525758749 2316 54-55 -0.30562242087795255 2316 56-57 -0.7156265583378527 2316 58-59 -0.5009004694272932 2316 60-61 -0.264717347917923 2316 62-63 -0.0705259018880966 2316 64-65 -0.3119089734304552 2316 66-67 -1.2931957091668522 2316 68-69 -0.7961668714123782 2316 70-71 -0.48948859002133815 2316 72-73 -0.3290343729671079 2316 74-75 -0.2646461625285177 2316 76-77 -0.26107982178797684 2316 78-79 -0.3799893085412904 2316 80-81 -0.8968054487456278 2316 82-83 -0.5708691571642142 2316 84-85 -0.7006980262204792 2316 86-87 -0.9922318581285126 2316 88-89 -0.5496150363436989 2316 90-91 -1.0003721287333924 2316 92-93 -0.783381518319402 2316 94-95 -0.6645639235298297 2316 96-97 -0.47835167910255194 2316 98-99 -0.7682842697915682 2316 100-101 -1.1531329585365917 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 4.0 4 19.0 5 34.0 6 65.0 7 208.0 8 426.0 9 905.0 10 1517.0 11 2446.0 12 3620.0 13 5019.0 14 6228.0 15 8126.0 16 10501.0 17 13734.0 18 12153.0 19 7752.0 20 6978.0 21 7257.0 22 7591.0 23 8533.0 24 9687.0 25 11802.0 26 14505.0 27 17777.0 28 22089.0 29 27218.0 30 33594.0 31 41525.0 32 51516.0 33 66329.0 34 87000.0 35 117693.0 36 167492.0 37 217114.0 38 131714.0 39 7376.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.94544315679458 19.01708605487624 15.153515426068964 21.883955362260206 2 15.720731228153578 21.244056398689814 39.04803041846678 23.987181954689824 3 20.55332988618192 24.593858453340616 30.253454320661355 24.599357339816105 4 12.8871360116643 17.16280443406593 37.58765741084819 32.36240214342158 5 13.389574061624748 37.575097513689165 33.90602855434958 15.129299870336505 6 30.834546143087604 36.491605227986064 17.293913247075288 15.379935381851045 7 28.044762498547737 33.16763498945946 20.726778655688303 18.060823856304502 8 26.54629369314559 33.07894720504882 19.6838989988036 20.69086010300199 9 25.947109449277008 17.454786366022905 20.99950688935874 35.59859729534134 10-11 25.126779974883462 26.283054142454752 27.035663353270472 21.554502529391314 12-13 27.02738178225479 23.802302006672946 27.572241467693324 21.598074743378938 14-15 23.15232969824253 25.27263181047389 27.33541872130892 24.23961976997467 16-17 21.47814341171556 28.600969038828072 27.808514167070953 22.112373382385417 18-19 21.177046537141113 27.308432168478408 30.440086523802474 21.074434770578005 20-21 22.27083298227752 25.606957024560305 30.382217744474527 21.739992248687642 22-23 21.686184933751417 25.674492154778548 30.59594238219973 22.04338052927031 24-25 22.341336005817976 25.85411604022917 30.58108803235362 21.223459921599233 26-27 21.97116879166044 25.53398312274084 31.226078021735827 21.268770063862895 28-29 21.10087349161165 26.76440028328499 30.93904006832898 21.195686156774382 30-31 21.52517592572473 25.84401998872763 30.61406935746147 22.016734728086167 32-33 21.655894762748353 25.99145490544744 30.657289382487214 21.695360949316992 34-35 22.62994622761794 25.614457564166525 30.27773158157326 21.477864626642276 36-37 22.491530407378647 25.331920662790246 30.81956031294585 21.356988616885257 38-39 21.85427820293125 26.033495471139823 30.835719278078756 21.276507047850167 40-41 21.859601323953555 26.13145347537808 30.529643504574594 21.47930169609377 42-43 21.585967744081167 26.44608419176175 29.701386640887943 22.26656142326914 44-45 22.062187842422702 25.97488061351505 29.481333403693277 22.481598140368973 46-47 22.623953428249997 26.58812177370326 29.027125614329467 21.760799183717268 48-49 22.607534987672278 26.62273622221828 28.876048743281835 21.89368004682761 50-51 21.918368903520598 26.891336507215733 29.300084431893968 21.8902101573697 52-53 22.506193911674178 27.426822708452235 28.121223731584006 21.945759648289584 54-55 21.89365145038689 26.721016541324044 28.72414822225445 22.66118378603462 56-57 22.591505325800643 26.839684975122612 28.337117415944586 22.23169228313216 58-59 23.25180027112156 26.468940971860384 28.15522485484975 22.124033902168307 60-61 22.379951006564976 26.704171537333103 29.456593271118887 21.459284184983034 62-63 22.16021066486441 26.910236829454764 29.02256185930014 21.906990646380684 64-65 21.88285945787009 27.546436631842425 28.53790079770379 22.032803112583693 66-67 22.041001853642097 27.12685475073392 28.15371925747887 22.678424138145118 68-69 21.878607244044485 27.006963602394844 28.469750258424458 22.64467889513621 70-71 22.52674158419442 26.93869279527046 28.217570646471867 22.316994974063256 72-73 22.348717489353984 26.504599194082314 28.877475589438728 22.26920772712498 74-75 21.933223323454325 27.835352373823092 27.95384729322038 22.27757700950221 76-77 22.230095998978282 27.019712428781244 27.956509553778584 22.79368201846189 78-79 22.155954698024523 27.114223009213745 28.2103644299936 22.51945786276813 80-81 22.564946511507245 27.25906452898951 27.864615553814215 22.311373405689032 82-83 22.262671272211563 27.059468381521118 28.41026659860938 22.267593747657944 84-85 22.0126051128225 27.410798782061573 28.093117477132612 22.483478627983313 86-87 22.123241958407892 27.347599440353527 27.798553877059597 22.730604724178992 88-89 21.9214830951211 27.31075032862467 27.45031602961081 23.317450546643418 90-91 22.069341120098493 26.71749529662945 28.02685511489884 23.186308468373216 92-93 22.439756832274313 26.900285534500274 28.054618284407262 22.605339348818152 94-95 21.928372065797443 26.8233585006399 28.17530671269094 23.07296272087172 96-97 21.357322515644732 26.954571738729406 28.74373855912361 22.944367186502248 98-99 21.740318126769115 27.204056029832202 27.562812276470243 23.49281356692844 100-101 21.154490884127846 26.888408172532824 28.132283917111018 23.82481702622831 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 223.0 1 184.5 2 269.0 3 364.5 4 291.0 5 255.5 6 371.5 7 760.5 8 1205.0 9 1476.5 10 1511.0 11 1391.5 12 1431.5 13 1589.0 14 1725.5 15 1874.0 16 2009.0 17 2150.0 18 2275.0 19 2383.0 20 2547.5 21 2832.0 22 3235.0 23 3741.5 24 4310.0 25 5176.5 26 6543.0 27 8073.0 28 9327.0 29 11013.5 30 13460.0 31 15786.5 32 18149.0 33 20117.0 34 22173.5 35 24209.5 36 26455.0 37 28514.0 38 29522.5 39 29879.5 40 30743.0 41 33515.5 42 35700.0 43 37108.5 44 38415.5 45 39957.0 46 41186.0 47 46948.5 48 61218.5 49 59385.5 50 44477.0 51 37934.5 52 35171.5 53 34487.5 54 33441.0 55 30864.5 56 27995.0 57 25267.0 58 23180.5 59 20680.0 60 17398.5 61 14253.0 62 11661.5 63 9123.5 64 6646.5 65 5106.5 66 4010.5 67 3236.5 68 2413.0 69 1607.0 70 1202.5 71 988.0 72 823.5 73 665.0 74 561.0 75 487.5 76 352.0 77 210.5 78 138.0 79 105.5 80 68.5 81 39.5 82 32.0 83 22.0 84 10.0 85 4.5 86 3.0 87 3.0 88 2.5 89 2.0 90 1.0 91 0.0 92 0.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03840181064980653 2 0.006917647184029813 3 0.004434389220531932 4 7.09502275285109E-4 5 0.0015076923349808568 6 3.547511376425545E-4 7 0.0 8 0.0 9 0.0013303167661595796 10-11 6.208144908744704E-4 12-13 0.0011529411973383022 14-15 0.0020398190414446884 16-17 0.003724886945246823 18-19 0.0 20-21 0.0 22-23 0.001552036227186176 24-25 9.755656285170248E-4 26-27 0.005099547603611721 28-29 0.005720362094486192 30-31 0.0013746606583648988 32-33 3.104072454372352E-4 34-35 0.010066063530607485 36-37 0.012017194787641535 38-39 0.010199095207223442 40-41 0.001685067903802134 42-43 5.321267064638318E-4 44-45 0.0013746606583648988 46-47 4.4343892205319315E-5 48-49 9.755656285170248E-4 50-51 0.0013303167661595796 52-53 0.0028823529933457554 54-55 0.0036805430530415036 56-57 0.0018624434726234114 58-59 0.001108597305132983 60-61 0.004700452573763847 62-63 0.00647420826197662 64-65 0.011307692512356426 66-67 0.0036361991608361843 68-69 0.004035294190684058 70-71 0.0 72-73 0.0014633484427755375 74-75 0.006784615507413855 76-77 0.0034588235920149065 78-79 0.0044787331127372505 80-81 0.0058090498788968305 82-83 0.0061638010165393844 84-85 0.006385520477565981 86-87 0.004744796465969167 88-89 0.01108597305132983 90-91 0.014899547780987291 92-93 0.0014633484427755375 94-95 0.002660633532319159 96-97 0.0013303167661595796 98-99 0.0039466064062734195 100-101 0.005942081555512789 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1127551.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.54389262113145 #Duplication Level Percentage of deduplicated Percentage of total 1 83.46642409864613 49.69915793999603 2 9.8356118844892 11.713012358262983 3 2.6867377509298187 4.799364724275159 4 1.1456158973216481 2.7285771990072547 5 0.6503479049468901 1.936212290926771 6 0.4178888197192413 1.4929636205360317 7 0.28878719307839945 1.2036859530512718 8 0.2150086292869198 1.0241960587901606 9 0.16127433672222716 0.8642611609499239 >10 1.0050261528341946 11.317356181062143 >50 0.08063153058189493 3.269584643870551 >100 0.04156260733479052 4.483570093792418 >500 0.00239209134526906 0.9756758468880756 >1k 0.0022425856361897437 2.782011486414165 >5k 4.4851712723794875E-4 1.7103704421770616 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8328 0.7385918685717985 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5765 0.5112850771273317 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5120 0.45408145618246976 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4353 0.38605792553950996 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3155 0.2798099598155649 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3029 0.2686352989798244 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2901 0.25728326257526263 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2250 0.1995475149239369 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2188 0.19404887229047735 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2156 0.1912108631893369 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1617 0.14340814739200267 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1531 0.13578099793268775 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1492 0.13232217434067284 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1426 0.12646878056957067 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1364 0.1209701379361111 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1333 0.1182208166193813 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1306 0.11582624644029406 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1150 0.10199095207223442 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 4.4343892205319315E-5 0.0 0.0 0.0 0.0 14-15 8.868778441063863E-5 0.0 0.0 0.0 0.0 16-17 8.868778441063863E-5 0.0 0.0 0.0 0.0 18-19 8.868778441063863E-5 0.0 0.0 0.0 0.0 20-21 8.868778441063863E-5 0.0 0.0 8.868778441063863E-5 0.0 22-23 8.868778441063863E-5 0.0 0.0 8.868778441063863E-5 0.0 24-25 1.7737556882127726E-4 0.0 0.0 3.104072454372352E-4 0.0 26-27 1.7737556882127726E-4 0.0 0.0 9.75565628517025E-4 0.0 28-29 1.7737556882127726E-4 0.0 0.0 0.0015963801193914955 0.0 30-31 1.7737556882127726E-4 0.0 0.0 0.003503167484220226 0.0 32-33 1.7737556882127726E-4 0.0 0.0 0.0051438914958170405 0.0 34-35 1.7737556882127726E-4 0.0 0.0 0.006740271615208536 0.0 36-37 1.7737556882127726E-4 0.0 0.0 0.008824434548858544 0.0 38-39 1.7737556882127726E-4 0.0 0.0 0.012504977601900048 0.0 40-41 1.7737556882127726E-4 0.0 0.0 0.01485520388878197 0.0 42-43 3.104072454372352E-4 0.0 0.0 0.02146244382737455 0.0 44-45 4.434389220531932E-4 0.0 0.0 0.02979909556197458 0.0 46-47 5.321267064638318E-4 0.0 0.0 0.037514932805700146 0.0 48-49 6.208144908744704E-4 0.0 0.0 0.04722624519866507 0.0 50-51 6.208144908744704E-4 0.0 0.0 0.06052941286026087 0.0 52-53 6.208144908744704E-4 0.0 0.0 0.0851846169264184 0.0 54-55 6.208144908744704E-4 0.0 0.0 0.10039457195284293 0.0 56-57 7.09502275285109E-4 0.0 0.0 0.1350271517651973 0.0 58-59 7.09502275285109E-4 0.0 0.0 0.18349502594561135 0.0 60-61 7.09502275285109E-4 0.0 0.0 0.2303221761144285 0.0 62-63 7.09502275285109E-4 0.0 0.0 0.3030018154389469 0.0 64-65 7.09502275285109E-4 0.0 0.0 0.3540859792594747 0.0 66-67 7.09502275285109E-4 0.0 0.0 0.4559438996550932 0.0 68-69 7.981900596957476E-4 0.0 0.0 0.5855167526790362 0.0 70-71 7.981900596957476E-4 0.0 0.0 0.666045260923896 0.0 72-73 7.981900596957476E-4 0.0 0.0 0.7956624578400445 0.0 74-75 7.981900596957476E-4 0.0 0.0 0.9321529580480173 0.0 76-77 8.868778441063864E-4 0.0 0.0 1.0206633668898348 0.0 78-79 9.312217363117057E-4 0.0 0.0 1.1136968527365947 0.0 80-81 0.0012416289817489409 0.0 0.0 1.284686901080306 0.0 82-83 0.0012416289817489409 0.0 0.0 1.4239710664972138 0.0 84-85 0.0012859728739542603 0.0 0.0 1.6421873600395902 0.0 86-87 0.0013303167661595796 0.0 0.0 1.9794226602610436 0.0 88-89 0.0013303167661595796 0.0 0.0 2.4312869218332476 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACGCA 65 1.3287966E-4 36.535526 4 TATCACG 75 3.076659E-4 31.66974 2 TACCTGG 1140 0.0 27.919378 2 ATAACGC 105 6.905325E-5 27.143085 3 GTACCTG 1240 0.0 26.82902 1 GAGTACT 6320 0.0 22.133303 12-13 GTACATG 4660 0.0 21.315172 1 AGTACTT 6545 0.0 20.973272 12-13 TACATGG 4830 0.0 20.752533 2 GTACTTT 6825 0.0 20.4956 14-15 CGTACAC 140 4.8513847E-4 20.357315 3 ACATGGG 4715 0.0 20.148632 3 TAAGGTG 445 0.0 19.213644 5 TATAACG 125 0.00602769 19.001846 2 GTACACG 155 9.5894746E-4 18.397041 1 TACTTTT 7635 0.0 18.103474 14-15 ACTTTTT 7840 0.0 18.052998 16-17 GTGTAGC 560 0.0 17.822134 1 AGAGTAC 10550 0.0 17.515142 10-11 CATGGGA 2630 0.0 17.337011 4 >>END_MODULE