FastQCFastQC Report
Thu 26 May 2016
SRR936492_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936492_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1205305
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT81520.6763433321856294No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT49080.40719983738555804No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43720.36272976549504066No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37300.30946523908886137No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT30300.25138865266467825No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC29640.2459128602303981No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT24540.20359991869277902No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20440.16958363235861462No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC19350.160540278186849No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT18730.15539635196070706No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC17730.14709969675725232No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG15200.12610915909251186No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC14650.12154599873061177No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA14110.11706580492074621No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA13570.11258561111088065No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA13350.11076034696612061No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA12800.1061971866042205No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGT459.5947366E-442.249486
TACCTGG11700.033.7141342
GTACCTG12800.031.5607131
ATAACGC950.00122257525.0140613
GTACATG51650.024.4764881
TACATGG53500.023.362632
ATCACGC1050.00218911622.6317713
ACATGGG53250.022.2237633
GAGTACT42450.022.012-13
TACCGTG1100.002866689621.6048497
TATAACG1100.002868762521.6021582
TCTATAC3351.8189894E-1221.280623
TAGACTG3850.020.9875665
AGTACTT44800.020.10337612-13
GTACTTT46450.020.05114-15
ACCTGGG18600.019.930563
GTGTAGC5500.019.0106891
GTACACG1801.2280783E-418.4826131
CATGGGG23550.018.3656524
GTATTAG4600.017.5642221
Produced by FastQC (version 0.11.5)