##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936492_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1205305 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.377983995752114 31.0 31.0 34.0 30.0 34.0 2 31.422663143353756 31.0 31.0 34.0 28.0 34.0 3 31.35626998975363 31.0 31.0 34.0 28.0 34.0 4 35.01957263929047 37.0 35.0 37.0 32.0 37.0 5 35.063768091893756 37.0 35.0 37.0 32.0 37.0 6 35.129868373565195 37.0 35.0 37.0 32.0 37.0 7 35.004654423569136 37.0 35.0 37.0 32.0 37.0 8 34.99847839343569 37.0 35.0 37.0 32.0 37.0 9 36.480389610928356 39.0 37.0 39.0 32.0 39.0 10-11 36.360229153616714 39.0 37.0 39.0 32.0 39.0 12-13 36.269053476091116 39.0 36.0 39.0 32.0 39.0 14-15 37.24935431280879 40.0 37.0 41.0 31.5 41.0 16-17 37.18254508195021 39.0 37.0 41.0 31.0 41.0 18-19 37.079507261647464 39.0 36.0 41.0 31.0 41.0 20-21 37.07704730337964 39.0 36.0 41.0 31.0 41.0 22-23 37.073853920791834 39.0 36.0 41.0 31.0 41.0 24-25 37.18414550673896 39.0 36.5 41.0 31.0 41.0 26-27 37.11357540207665 39.0 36.0 41.0 31.0 41.0 28-29 37.01160453163307 39.0 36.0 41.0 30.5 41.0 30-31 36.83063208067667 39.0 36.0 41.0 30.0 41.0 32-33 36.66374112776434 39.0 35.5 41.0 30.0 41.0 34-35 36.44282277099987 39.0 35.0 41.0 30.0 41.0 36-37 36.235788451885625 39.0 35.0 40.0 30.0 41.0 38-39 36.050174022342894 39.0 35.0 40.0 29.5 41.0 40-41 35.85158486856024 38.0 35.0 40.0 28.5 41.0 42-43 35.581552387155114 38.0 35.0 40.0 27.0 41.0 44-45 35.411041603577516 38.0 35.0 40.0 27.0 41.0 46-47 35.243461613450535 38.0 35.0 40.0 26.0 41.0 48-49 34.982545496782976 38.0 34.0 40.0 24.5 41.0 50-51 33.982843346704776 37.5 33.0 39.5 23.5 40.5 52-53 34.088430314318785 37.5 33.5 39.5 24.0 40.5 54-55 34.533360435740335 38.0 34.0 40.0 24.0 41.0 56-57 34.37011544795716 38.0 34.0 40.0 23.0 41.0 58-59 34.15081950211772 38.0 33.5 40.0 23.0 41.0 60-61 33.82905198269317 37.5 33.0 40.0 22.0 41.0 62-63 33.52326672501981 37.0 33.0 40.0 22.0 41.0 64-65 33.17324619079818 36.5 32.5 39.5 20.5 41.0 66-67 32.78472751710149 36.0 32.0 39.0 20.0 41.0 68-69 32.34701673020522 35.5 31.5 39.0 18.5 40.0 70-71 31.93337910321454 35.0 31.0 38.5 17.5 40.0 72-73 31.51226577505279 35.0 31.0 37.5 13.5 40.0 74-75 31.10914374369973 35.0 31.0 37.0 11.0 39.0 76-77 30.655081908728498 35.0 30.0 36.5 7.5 39.0 78-79 30.173552337375185 34.0 30.0 36.0 7.0 38.5 80-81 29.69958599690535 34.0 29.0 35.5 2.0 37.0 82-83 29.30067534773356 34.0 29.0 35.0 2.0 37.0 84-85 28.934775015452523 34.0 29.0 35.0 2.0 36.5 86-87 28.621561347542738 34.0 29.0 35.0 2.0 36.0 88-89 28.26061868157852 34.0 28.0 35.0 2.0 36.0 90-91 27.896635706315 34.0 27.0 35.0 2.0 35.0 92-93 27.48225262485429 33.5 26.5 35.0 2.0 35.0 94-95 27.00163693007164 33.0 25.5 35.0 2.0 35.0 96-97 26.413050223802273 33.0 25.0 35.0 2.0 35.0 98-99 25.70335973052464 33.0 22.0 35.0 2.0 35.0 100-101 23.14781528326855 29.5 8.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6555700803865108 1101 2 0.6535843501500018 1101 3 0.7128519938104567 1101 4 0.8366390542813349 1101 5 0.6622494043553928 1101 6 0.6100509315214637 1101 7 0.7958400726572279 1101 8 0.7381306031956143 1101 9 0.9363661240908598 1101 10-11 0.9731925530833578 1101 12-13 1.002323787413495 1101 14-15 1.2460952666740468 1101 16-17 1.296210061779206 1101 18-19 1.3712721045699183 1101 20-21 1.1839042175395633 1101 22-23 1.1723902245429727 1101 24-25 1.0384882399753863 1101 26-27 0.928949194488979 1101 28-29 1.244894064564626 1101 30-31 0.9996535874270549 1101 32-33 1.112362624430979 1101 34-35 1.139927031883552 1101 36-37 1.059982420938148 1101 38-39 1.0963970227436661 1101 40-41 0.9103925705154481 1101 42-43 0.9299141090043364 1101 44-45 0.7579392521984119 1101 46-47 0.9242171700796007 1101 48-49 0.9392418358905985 1101 50-51 0.7610281625142505 1101 52-53 1.0634142970750275 1101 54-55 0.862954309721573 1101 56-57 1.155840350002677 1101 58-59 1.0227585663138683 1101 60-61 0.9720144323280593 1101 62-63 1.1114393267942901 1101 64-65 1.113863180152471 1101 66-67 0.9050616204847728 1101 68-69 0.9851089873382577 1101 70-71 1.0126658808352538 1101 72-73 1.0023578447952879 1101 74-75 0.9292726970972005 1101 76-77 0.9097370620005627 1101 78-79 1.0143869020350067 1101 80-81 1.0591037179714071 1101 82-83 1.2733582828088288 1101 84-85 1.3456165040726624 1101 86-87 1.179756157587967 1101 88-89 1.1771811995043322 1101 90-91 1.1788995667530635 1101 92-93 1.378401128085308 1101 94-95 1.445536065729474 1101 96-97 1.6042844163793895 1101 98-99 1.4220528509476544 1101 100-101 1.484674414012833 1102 1 0.35809252037911676 1102 2 0.4491232715839111 1102 3 0.6127921270441448 1102 4 0.5774581441457656 1102 5 0.6143981792965292 1102 6 0.45247075425155003 1102 7 0.6545906652980378 1102 8 0.5425852531156607 1102 9 0.5852374949192765 1102 10-11 0.6051766743276232 1102 12-13 0.563220141567605 1102 14-15 0.8347272106162578 1102 16-17 0.857834660993305 1102 18-19 0.8862398006235352 1102 20-21 0.8454962717330474 1102 22-23 0.460422697241313 1102 24-25 0.6889075082347489 1102 26-27 0.6545524827414368 1102 28-29 0.4479518902358137 1102 30-31 0.36159841357235223 1102 32-33 0.5040308982591668 1102 34-35 0.23281494040391237 1102 36-37 0.2555127038789351 1102 38-39 0.09080811858674309 1102 40-41 0.4310345511812912 1102 42-43 0.3577505481772931 1102 44-45 0.3713144520891234 1102 46-47 0.41782970754655224 1102 48-49 0.5073211975260463 1102 50-51 0.5610370019025694 1102 52-53 0.4785039365083321 1102 54-55 0.6488974724547134 1102 56-57 0.6645391116847321 1102 58-59 0.5717870371800444 1102 60-61 0.658267375442577 1102 62-63 0.7194747325623965 1102 64-65 0.792553423878509 1102 66-67 0.643644746896058 1102 68-69 0.7740605555261055 1102 70-71 0.4785398150862825 1102 72-73 0.6595081063608355 1102 74-75 0.3616322914496344 1102 76-77 0.3838416721433582 1102 78-79 0.3501189239245477 1102 80-81 0.5505074128357208 1102 82-83 0.3022992437306975 1102 84-85 0.2570779763933224 1102 86-87 0.2797306438989686 1102 88-89 0.3236930563383851 1102 90-91 0.5283376629095393 1102 92-93 0.4288921159271304 1102 94-95 0.4233692905794051 1102 96-97 0.6180085712133341 1102 98-99 0.5189225021132486 1102 100-101 1.2311989272437884 1103 1 0.27880901555898063 1103 2 0.44142499222024867 1103 3 0.5017937782205166 1103 4 0.44471630208667534 1103 5 0.44328992029016945 1103 6 0.40783491435076513 1103 7 0.4729180020010162 1103 8 0.29083793838029237 1103 9 0.4107067102139297 1103 10-11 0.3560404174266836 1103 12-13 0.3056465416425951 1103 14-15 0.5329161441293735 1103 16-17 0.697523040509104 1103 18-19 0.810074783931519 1103 20-21 0.6410168849996651 1103 22-23 0.4836256909899177 1103 24-25 0.4093557773837091 1103 26-27 0.37737987592571187 1103 28-29 0.3932876174143658 1103 30-31 0.49027480463437456 1103 32-33 0.3779935052431327 1103 34-35 0.3009112446049187 1103 36-37 0.3418061974749165 1103 38-39 0.2400719390771897 1103 40-41 0.36158119390950816 1103 42-43 0.5073857030922468 1103 44-45 0.4704673529440413 1103 46-47 0.6309966974784658 1103 48-49 0.7084940254600127 1103 50-51 0.5995785709287063 1103 52-53 0.7925498113313836 1103 54-55 0.7456610345733097 1103 56-57 0.8763739844335845 1103 58-59 0.6180124648155001 1103 60-61 0.5787705636310179 1103 62-63 0.7488125555572509 1103 64-65 0.61183763771907 1103 66-67 0.6495749816899732 1103 68-69 0.6801027314256629 1103 70-71 0.6185383009070655 1103 72-73 0.6709089144037712 1103 74-75 0.6219446329715552 1103 76-77 0.6511402682829193 1103 78-79 0.3361124658300554 1103 80-81 0.7173083727772678 1103 82-83 0.5856313152480261 1103 84-85 0.6809766513663433 1103 86-87 0.6850304513818983 1103 88-89 0.583706167207545 1103 90-91 0.8323283663299001 1103 92-93 0.9438542494296662 1103 94-95 0.9622897048396091 1103 96-97 1.018966229475101 1103 98-99 1.1907824429400833 1103 100-101 1.479785392885141 1104 1 0.17893649434373415 1104 2 0.1381731263142676 1104 3 0.432025862327869 1104 4 0.5089437938727244 1104 5 0.48741388652206297 1104 6 0.3444315723572444 1104 7 0.2772220912383716 1104 8 0.4567482162333576 1104 9 0.619856756159173 1104 10-11 0.6195835233255309 1104 12-13 0.5600997601238191 1104 14-15 0.4910935721209313 1104 16-17 0.4769949345772133 1104 18-19 0.5022675071736415 1104 20-21 0.7962751920549564 1104 22-23 0.33301452001409615 1104 24-25 0.48212500883096965 1104 26-27 0.2738920585784541 1104 28-29 0.31459453388216474 1104 30-31 0.3893197604630245 1104 32-33 0.3431985180130468 1104 34-35 0.256451503440843 1104 36-37 0.455097871578765 1104 38-39 0.316847331815417 1104 40-41 0.40827622102492 1104 42-43 0.4658515284209699 1104 44-45 0.661207897808417 1104 46-47 0.6641318161758676 1104 48-49 0.6188828853347133 1104 50-51 0.4809811570843081 1104 52-53 0.5509298611770248 1104 54-55 0.7049694649205307 1104 56-57 0.6709513432318275 1104 58-59 0.7811023985089065 1104 60-61 0.8962467260898919 1104 62-63 0.7691893286361946 1104 64-65 0.6893057900589739 1104 66-67 0.7926980135916182 1104 68-69 0.7474037069633965 1104 70-71 0.5800814663033584 1104 72-73 0.8897713673291641 1104 74-75 0.7167821829108441 1104 76-77 0.7353933329522349 1104 78-79 0.6473245940536643 1104 80-81 0.6094116845813424 1104 82-83 0.6803162652591546 1104 84-85 0.633985238830892 1104 86-87 0.3940314782637273 1104 88-89 0.5986948406569184 1104 90-91 0.4393367246191069 1104 92-93 0.5301944073402929 1104 94-95 0.49208470308773755 1104 96-97 0.5960336004152289 1104 98-99 0.4545164364746981 1104 100-101 1.3284495099761102 1105 1 0.3720808054018434 1105 2 0.2879202677956272 1105 3 0.31086675040069167 1105 4 0.36550377042457427 1105 5 0.2691863619301955 1105 6 0.26659570837099267 1105 7 0.38615260024828046 1105 8 0.2976729914056122 1105 9 0.22465773378573317 1105 10-11 0.42149318281559545 1105 12-13 0.4318723440881982 1105 14-15 0.6254644964153613 1105 16-17 0.5648657898608676 1105 18-19 0.5560173243080584 1105 20-21 0.39550284019417603 1105 22-23 0.35451175880837127 1105 24-25 0.285642334666683 1105 26-27 0.2849417923313382 1105 28-29 0.4060177631617492 1105 30-31 0.24215418995600402 1105 32-33 0.21735985637718613 1105 34-35 0.299598993646228 1105 36-37 0.32204642263716465 1105 38-39 0.20852706400197718 1105 40-41 0.12159209231029422 1105 42-43 -0.05524637845948632 1105 44-45 0.093972034545871 1105 46-47 0.31595315740382546 1105 48-49 0.2978670676554458 1105 50-51 0.41955770552288385 1105 52-53 0.5946563180406486 1105 54-55 0.39240216650819804 1105 56-57 0.4225733545103054 1105 58-59 0.4731608886155527 1105 60-61 0.30725050436426926 1105 62-63 0.4510228540107022 1105 64-65 0.5391740492974932 1105 66-67 0.3917343959598085 1105 68-69 0.4377358942529028 1105 70-71 0.5800411454110908 1105 72-73 0.6061330167845007 1105 74-75 0.5924640841546385 1105 76-77 0.47784311664098666 1105 78-79 0.47014218698944177 1105 80-81 0.5261418049598809 1105 82-83 0.5048260433339955 1105 84-85 0.3128735297988605 1105 86-87 0.44044030833235936 1105 88-89 0.4005902844477873 1105 90-91 0.47798688453280036 1105 92-93 0.49434499863584236 1105 94-95 0.4250799095539719 1105 96-97 0.6655631021265371 1105 98-99 0.8374703140263478 1105 100-101 1.1080120903271222 1106 1 0.38135506198544533 1106 2 0.28331185778448287 1106 3 0.32410013412836136 1106 4 0.4275677207913944 1106 5 0.5211680481577616 1106 6 0.36177358440691165 1106 7 0.43130323137619797 1106 8 0.5316275885530644 1106 9 0.6535769924645223 1106 10-11 0.6844132875599058 1106 12-13 0.7500823811023167 1106 14-15 0.598505023153578 1106 16-17 0.3719089107338718 1106 18-19 0.6882500232920279 1106 20-21 0.29466296783319734 1106 22-23 0.27395823018074594 1106 24-25 0.015398818630153244 1106 26-27 0.03506852735250732 1106 28-29 0.22086501437278372 1106 30-31 0.10222973651482903 1106 32-33 0.25823021506601407 1106 34-35 0.31209444242305295 1106 36-37 0.3355187924047556 1106 38-39 0.2656085175365561 1106 40-41 0.20467257991173682 1106 42-43 0.21143082656756462 1106 44-45 0.21226937513318944 1106 46-47 0.3311253996622412 1106 48-49 0.3047574572201768 1106 50-51 0.2093094247490015 1106 52-53 0.3218513810935022 1106 54-55 0.4983881050390906 1106 56-57 0.5479396374508028 1106 58-59 0.7402239175001455 1106 60-61 0.7715896417869956 1106 62-63 0.6882735606052748 1106 64-65 0.445258528989676 1106 66-67 0.5812028780213723 1106 68-69 0.6110192527834073 1106 70-71 0.4969466732555823 1106 72-73 0.6310282699773531 1106 74-75 0.6605486347684888 1106 76-77 0.2939669578583626 1106 78-79 0.480090449133904 1106 80-81 0.6702344046834874 1106 82-83 0.5635861428041302 1106 84-85 0.9808447555799837 1106 86-87 0.9001480611194097 1106 88-89 0.9228452038892669 1106 90-91 0.8662870070474824 1106 92-93 0.9098982700475666 1106 94-95 0.973724931127645 1106 96-97 1.188019049532631 1106 98-99 1.222322994241054 1106 100-101 1.6724631541271542 1107 1 0.10752759883338925 1107 2 0.15268439313302196 1107 3 0.12755308271367127 1107 4 0.12941611567541855 1107 5 0.17986348845884237 1107 6 0.23720232130582986 1107 7 0.18995856252039545 1107 8 0.3439068944772288 1107 9 0.3314718802338561 1107 10-11 0.40302388949714185 1107 12-13 0.19884574457374526 1107 14-15 -0.018506647861372016 1107 16-17 0.10443190789998624 1107 18-19 0.20494124276033432 1107 20-21 0.14200920357007618 1107 22-23 0.11553174614194006 1107 24-25 0.09355240109007212 1107 26-27 0.1441900337324853 1107 28-29 0.16062607437908127 1107 30-31 0.07930426082772613 1107 32-33 -0.0010129307663788722 1107 34-35 -0.0039675880749001635 1107 36-37 0.0016016271850176622 1107 38-39 -7.591578566703561E-4 1107 40-41 0.2040707082760349 1107 42-43 0.022553714276924097 1107 44-45 0.08494154462623982 1107 46-47 0.04270143650269631 1107 48-49 0.1251031081880427 1107 50-51 -0.029481096745008983 1107 52-53 0.02968381853071378 1107 54-55 -0.13314251848961334 1107 56-57 0.20861991873968577 1107 58-59 0.39599223276562157 1107 60-61 0.24719755791011977 1107 62-63 0.27671800005168734 1107 64-65 0.049791613421326986 1107 66-67 -0.19776966220024406 1107 68-69 -0.11003796086719575 1107 70-71 0.1875687212966035 1107 72-73 0.09539929654685153 1107 74-75 -0.07871162305466939 1107 76-77 -0.00121980274619915 1107 78-79 -0.14261194279628597 1107 80-81 -0.007188638214568499 1107 82-83 -0.0021225897465093624 1107 84-85 -0.046155829189032715 1107 86-87 0.01966678872200589 1107 88-89 0.1329635352733689 1107 90-91 0.0013248389788707016 1107 92-93 0.11367832380133436 1107 94-95 0.12496667501380543 1107 96-97 0.23911230519664173 1107 98-99 0.4085602117901246 1107 100-101 0.9140221914324158 1108 1 0.1413710727664501 1108 2 0.15398307423293645 1108 3 0.33430777973992676 1108 4 0.2549449199556193 1108 5 0.17788824894160626 1108 6 0.279619423458378 1108 7 0.2814417029956999 1108 8 0.456188373884963 1108 9 -0.006461272683900177 1108 10-11 0.3168151828901955 1108 12-13 0.1733542676544957 1108 14-15 0.6344635991213323 1108 16-17 0.720718302076925 1108 18-19 0.5049443533737517 1108 20-21 0.2579339889377579 1108 22-23 0.3444728933361674 1108 24-25 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-3.963471645179421 2316 86-87 -4.920308698624428 2316 88-89 -6.160823161041016 2316 90-91 -5.145457390800612 2316 92-93 -4.5877632479151345 2316 94-95 -4.27813507187609 2316 96-97 -5.758075521800219 2316 98-99 -7.429909394457212 2316 100-101 -8.325986843369737 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2778.0 3 1005.0 4 1055.0 5 1174.0 6 1277.0 7 1583.0 8 1884.0 9 2488.0 10 3003.0 11 4058.0 12 5076.0 13 6182.0 14 6910.0 15 8154.0 16 9840.0 17 12469.0 18 10461.0 19 8289.0 20 8010.0 21 8741.0 22 9604.0 23 10730.0 24 12300.0 25 14250.0 26 16663.0 27 19773.0 28 22900.0 29 28055.0 30 33908.0 31 41749.0 32 51677.0 33 65602.0 34 87069.0 35 116967.0 36 161933.0 37 213369.0 38 169555.0 39 24754.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.21826932338669 18.482892853228286 14.250613638552775 22.04822418483225 2 15.455173192221123 20.759579186391612 39.646727042154396 24.138520579232868 3 20.455062193492708 24.729194845283832 29.62044958551501 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21.900853359196976 48-49 22.756662084094216 27.234916713059338 27.86053326541059 22.147887937435858 50-51 22.06098861643728 27.49075845200804 28.43032447364349 22.017928457911186 52-53 22.609552373781685 27.98604499700283 27.314429365242336 22.089973263973153 54-55 22.022853184159164 27.33251611110074 27.726442117227833 22.918188587512265 56-57 22.65908428088956 27.52177038862078 27.351224787896616 22.46792054259305 58-59 23.33866279913943 27.0851500395354 27.288137140126228 22.288050021198938 60-61 22.582335385235762 27.211478705600538 28.50916386428072 21.69702204488298 62-63 22.230754232471696 27.45952135007654 28.181976577765056 22.127747839686705 64-65 22.004299365963824 28.081405864461285 27.684697675421933 22.22959709415296 66-67 22.20828834090893 27.7029989741105 27.20785068889745 22.88086199608312 68-69 21.899522595012222 27.621288578620817 27.70434078452391 22.774848041843047 70-71 22.606084314882978 27.54663959687359 27.30918430123469 22.53809178700874 72-73 22.421042760845804 27.103458691789804 28.025784800809262 22.449713746555126 74-75 21.853232514961984 28.5274501872193 27.27423219342191 22.3450851043968 76-77 22.277675727284382 27.629814935683296 27.231645122648 22.860864214384318 78-79 22.381895423941685 27.752059950268265 27.419878836461585 22.446165789328465 80-81 22.702136442747108 27.84275084376938 27.143879534417703 22.311233179065805 82-83 22.40643725616768 27.657699666620754 27.686200356113417 22.249662721098144 84-85 22.131301238986804 27.945155356649483 27.39361415264271 22.529929251720997 86-87 22.28329137911294 28.003944305150164 27.168662603440524 22.544101712296374 88-89 22.155441704175395 27.903590466905893 26.785090539503432 23.155877289415276 90-91 22.221355614374257 27.443930933198363 27.305656275669797 23.02905717675758 92-93 22.740541473468994 27.710064091287766 27.300117163255212 22.249277271988024 94-95 22.18964359106594 27.57500610935771 27.42182615236593 22.81352414721042 96-97 21.60544996660153 27.5890038128192 27.947549879889973 22.857996340689294 98-99 21.941466462904817 28.064612367702445 26.92789179100607 23.066029378386666 100-101 21.717568828599536 27.528825794651713 27.274643466339928 23.47896191040882 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 138.0 1 117.5 2 192.5 3 280.5 4 247.0 5 227.5 6 325.0 7 604.0 8 986.0 9 1262.0 10 1244.5 11 1140.0 12 1211.0 13 1317.5 14 1412.0 15 1526.0 16 1628.0 17 1759.5 18 1824.5 19 1899.0 20 2162.0 21 2482.5 22 2853.5 23 3482.5 24 4166.5 25 5005.0 26 6362.0 27 8038.0 28 9532.0 29 11254.5 30 13698.0 31 16529.5 32 19553.0 33 21728.0 34 23970.5 35 26180.0 36 28405.0 37 30609.5 38 31522.5 39 32157.5 40 33350.5 41 36037.0 42 38365.0 43 39958.0 44 41743.5 45 43622.0 46 44694.0 47 50033.0 48 63915.5 49 63116.5 50 48901.0 51 41911.0 52 39174.5 53 37874.5 54 36319.0 55 33807.5 56 30722.0 57 27310.0 58 24685.0 59 22260.0 60 18862.5 61 15418.5 62 12680.0 63 10057.5 64 7613.0 65 5897.0 66 4378.0 67 3478.0 68 2648.5 69 1764.0 70 1296.5 71 1002.5 72 838.0 73 723.5 74 570.5 75 449.5 76 326.5 77 197.5 78 116.0 79 79.0 80 52.0 81 32.5 82 18.5 83 10.0 84 8.5 85 7.0 86 5.5 87 3.0 88 1.5 89 1.0 90 0.5 91 0.0 92 1.0 93 1.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08404511721099639 2 0.033269587365853455 3 0.016178477646736716 4 0.023562500777811426 5 0.02820862769174607 6 0.03584155047892442 7 0.08462588307523822 8 0.06239084712997955 9 0.07865229132875082 10-11 0.06811553922036331 12-13 0.04409672240636187 14-15 0.04417968895839642 16-17 0.05596093934730214 18-19 0.053886775546438455 20-21 0.021322403872878648 22-23 0.015265845574356698 24-25 0.02534628164655419 26-27 0.02771082837953879 28-29 0.007218090027005613 30-31 0.0010370819004318409 32-33 0.010578235384404777 34-35 0.013274648325527563 36-37 0.005143926226141931 38-39 0.010536752108387504 40-41 0.00547579243428012 42-43 0.0026134463890882392 44-45 2.488996561036418E-4 46-47 7.881822443281991E-4 48-49 0.0011615317284836618 50-51 0.0012030150045009354 52-53 2.0741638008636818E-4 54-55 2.0741638008636818E-4 56-57 0.004604643637917374 58-59 0.004646126913934647 60-61 0.006803257266832876 62-63 0.003318662081381891 64-65 9.1263207238002E-4 66-67 7.881822443281991E-4 68-69 0.0032356955293473435 70-71 0.0021156470768809556 72-73 0.021073504216775008 74-75 0.013896897465786668 76-77 0.024184749918070528 78-79 0.01987048921227407 80-81 0.012237566425095723 82-83 0.005890625194452857 84-85 0.0038164613935891744 86-87 0.0019082306967945872 88-89 0.002488996561036418 90-91 0.007881822443281991 92-93 0.012362016253147543 94-95 0.015473261954443067 96-97 0.013274648325527563 98-99 0.025180348542485097 100-101 0.021944653013137753 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1205305.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.189018273429284 #Duplication Level Percentage of deduplicated Percentage of total 1 84.52419809923303 52.5647690014016 2 9.295212473298529 11.561202767147401 3 2.5095425668379003 4.681979656410925 4 1.0691083899878362 2.6594720480492042 5 0.5932662661732933 1.8447323334030048 6 0.37581036865706346 1.402276673025499 7 0.26391267616926767 1.148872916861615 8 0.19393783599060424 0.9648642901063216 9 0.13759955467775925 0.7701463098243811 >10 0.9250849960005115 10.8301902965636 >50 0.07370421559609278 3.1577686255774347 >100 0.03446527544991436 3.7734812680511904 >500 0.0017433762924693272 0.7477565382652392 >1k 0.002279799767075274 3.212617623300834 >5k 1.341058686514867E-4 0.6798696520118481 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8152 0.6763433321856294 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4908 0.40719983738555804 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4372 0.36272976549504066 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3730 0.30946523908886137 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3030 0.25138865266467825 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2964 0.2459128602303981 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2454 0.20359991869277902 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2044 0.16958363235861462 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1935 0.160540278186849 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1873 0.15539635196070706 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1773 0.14709969675725232 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1520 0.12610915909251186 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1465 0.12154599873061177 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1411 0.11706580492074621 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1357 0.11258561111088065 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1335 0.11076034696612061 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1280 0.1061971866042205 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 8.296655203454727E-5 8.296655203454727E-5 0.0 0.0 0.0 16-17 8.296655203454727E-5 8.296655203454727E-5 0.0 0.0 0.0 18-19 1.6593310406909454E-4 8.296655203454727E-5 0.0 0.0 0.0 20-21 2.488996561036418E-4 8.296655203454727E-5 0.0 4.1483276017273635E-5 0.0 22-23 2.488996561036418E-4 8.296655203454727E-5 0.0 1.244498280518209E-4 0.0 24-25 2.488996561036418E-4 8.296655203454727E-5 0.0 4.1483276017273635E-4 0.0 26-27 2.488996561036418E-4 8.296655203454727E-5 0.0 5.392825882245573E-4 0.0 28-29 4.977993122072836E-4 8.296655203454727E-5 0.0 7.881822443281991E-4 0.0 30-31 4.977993122072836E-4 8.296655203454727E-5 0.0 0.0019911972488291345 0.0 32-33 4.977993122072836E-4 8.296655203454727E-5 0.0 0.0035675617374855326 0.0 34-35 4.977993122072836E-4 1.6593310406909454E-4 0.0 0.005434309158262846 0.0 36-37 5.807658642418309E-4 1.6593310406909454E-4 0.0 0.007632922787178349 0.0 38-39 5.807658642418309E-4 1.6593310406909454E-4 0.0 0.011076034696612062 0.0 40-41 5.807658642418309E-4 1.6593310406909454E-4 0.0 0.014145797121890308 0.0 42-43 5.807658642418309E-4 1.6593310406909454E-4 0.0 0.020326805248464083 0.0 44-45 6.637324162763782E-4 1.6593310406909454E-4 0.0 0.02795972803564243 0.0 46-47 6.637324162763782E-4 1.6593310406909454E-4 0.0 0.037625331347667185 0.0 48-49 6.637324162763782E-4 1.6593310406909454E-4 0.0 0.048659882768261976 0.0 50-51 6.637324162763782E-4 1.6593310406909454E-4 0.0 0.060980415745392244 0.0 52-53 6.637324162763782E-4 1.6593310406909454E-4 0.0 0.08201243668614998 0.0 54-55 6.637324162763782E-4 1.6593310406909454E-4 0.0 0.09902057985323218 0.0 56-57 6.637324162763782E-4 1.6593310406909454E-4 0.0 0.13370059860367292 0.0 58-59 6.637324162763782E-4 1.6593310406909454E-4 0.0 0.18012038446700213 0.0 60-61 6.637324162763782E-4 1.6593310406909454E-4 0.0 0.2240511737692949 0.0 62-63 6.637324162763782E-4 1.6593310406909454E-4 0.0 0.29104666453719186 0.0 64-65 6.637324162763782E-4 1.6593310406909454E-4 0.0 0.3370516176403483 0.0 66-67 6.637324162763782E-4 1.6593310406909454E-4 0.0 0.4265725272856248 0.0 68-69 6.637324162763782E-4 1.6593310406909454E-4 0.0 0.544550964278751 0.0 70-71 7.466989683109254E-4 1.6593310406909454E-4 0.0 0.6188475116256881 0.0 72-73 8.711487963627463E-4 1.6593310406909454E-4 0.0 0.7437951389897164 0.0 74-75 9.955986244145672E-4 1.6593310406909454E-4 0.0 0.8784083696657692 0.0 76-77 0.0010370819004318409 1.6593310406909454E-4 0.0 0.9605867394559884 0.0 78-79 0.0010785651764491145 1.6593310406909454E-4 0.0 1.0529285118704395 0.0 80-81 0.001244498280518209 1.6593310406909454E-4 0.0 1.2242959250977967 0.0 82-83 0.001244498280518209 1.6593310406909454E-4 0.0 1.3651731304524581 0.0 84-85 0.001244498280518209 1.6593310406909454E-4 0.0 1.574041425199431 0.0 86-87 0.0012859815565354827 1.6593310406909454E-4 0.0 1.9005148074553744 0.0 88-89 0.0013274648325527563 1.6593310406909454E-4 0.0 2.3268384350848956 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACGGT 45 9.5947366E-4 42.24948 6 TACCTGG 1170 0.0 33.714134 2 GTACCTG 1280 0.0 31.560713 1 ATAACGC 95 0.001222575 25.014061 3 GTACATG 5165 0.0 24.476488 1 TACATGG 5350 0.0 23.36263 2 ATCACGC 105 0.002189116 22.631771 3 ACATGGG 5325 0.0 22.223763 3 GAGTACT 4245 0.0 22.0 12-13 TACCGTG 110 0.0028666896 21.604849 7 TATAACG 110 0.0028687625 21.602158 2 TCTATAC 335 1.8189894E-12 21.28062 3 TAGACTG 385 0.0 20.987566 5 AGTACTT 4480 0.0 20.103376 12-13 GTACTTT 4645 0.0 20.051 14-15 ACCTGGG 1860 0.0 19.93056 3 GTGTAGC 550 0.0 19.010689 1 GTACACG 180 1.2280783E-4 18.482613 1 CATGGGG 2355 0.0 18.365652 4 GTATTAG 460 0.0 17.564222 1 >>END_MODULE