FastQCFastQC Report
Thu 26 May 2016
SRR936492_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936492_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1205305
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT89000.7384023131074707No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59110.4904152890762089No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55340.45913689895918464No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT47230.3918510252591667No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC32690.271217658600935No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32530.26989019376838225No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT32160.266820431343104No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC23850.19787522660239523No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT22620.18767034070214592No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT22570.1872555079419732No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG17020.14120907156279947No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16920.14037940604245397No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC16640.13805634258548666No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA15250.1265239918526846No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA14700.12196083149078449No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA14410.11955480148178263No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC14230.11806140354516077No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA12560.10420598935539137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG13450.036.3846782
GTACCTG14500.034.7415851
GAGTACT64000.023.08021512-13
GTACATG51650.022.8188171
TACATGG52400.022.2145962
ACCTGGG21800.022.0088043
AGTACTT66550.021.51784112-13
ACATGGG52000.021.1941593
GTACTTT70350.020.82814214-15
GTACACG1951.0282451E-519.4969711
TGCACCG2001.2856899E-518.9985075
TACTTTT77250.018.47589314-15
AGAGTAC108850.018.37016910-11
ACTTTTT81550.018.22971316-17
CATGGGG24250.018.2150634
ATACGTC2905.2183168E-818.0158255
CCACGTC3451.0841177E-917.8971449
GTATTAG3501.3023964E-917.6517221
GTATGCT3401.751323E-816.7633884
CATGGGA29100.016.4849594
Produced by FastQC (version 0.11.5)