##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936492_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1205305 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.906163999983406 31.0 31.0 34.0 30.0 34.0 2 32.062916025404355 33.0 31.0 34.0 30.0 34.0 3 32.14503714827367 33.0 31.0 34.0 30.0 34.0 4 35.84023297007811 37.0 35.0 37.0 35.0 37.0 5 35.675380090516505 37.0 35.0 37.0 33.0 37.0 6 35.77238956114842 37.0 35.0 37.0 33.0 37.0 7 35.72315140151248 37.0 35.0 37.0 33.0 37.0 8 35.69997469520163 37.0 35.0 37.0 33.0 37.0 9 37.32508701117145 39.0 37.0 39.0 34.0 39.0 10-11 37.220344643057146 39.0 37.0 39.0 33.0 39.0 12-13 37.11823521847167 39.0 37.0 39.0 33.0 39.0 14-15 38.231691148713395 40.0 38.0 41.0 33.0 41.0 16-17 38.14893782071758 40.0 37.0 41.0 33.0 41.0 18-19 38.31802531309503 40.0 38.0 41.0 33.5 41.0 20-21 38.33967502001568 40.0 38.0 41.0 34.0 41.0 22-23 38.25640149173861 40.0 38.0 41.0 33.5 41.0 24-25 38.115160063220515 40.0 37.0 41.0 33.0 41.0 26-27 37.906780856297786 40.0 37.0 41.0 33.0 41.0 28-29 37.82533176249995 40.0 37.0 41.0 33.0 41.0 30-31 37.78552441083377 40.0 37.0 41.0 33.0 41.0 32-33 37.60584374909256 40.0 36.5 41.0 33.0 41.0 34-35 37.402769008674156 39.5 36.0 41.0 32.0 41.0 36-37 37.18229701195963 39.0 36.0 41.0 31.5 41.0 38-39 37.00945528310262 39.0 36.0 41.0 31.0 41.0 40-41 36.82980863764773 39.0 35.5 41.0 31.0 41.0 42-43 36.54279663653598 39.0 35.0 41.0 30.0 41.0 44-45 36.2326149812703 39.0 35.0 40.0 30.0 41.0 46-47 35.81209942711596 39.0 35.0 40.0 28.5 41.0 48-49 35.37900614367318 38.0 35.0 40.0 27.0 41.0 50-51 35.07095755846031 38.0 34.5 40.0 26.0 41.0 52-53 34.7178307565305 38.0 34.0 40.0 25.0 41.0 54-55 34.53625513874081 38.0 34.0 40.0 25.0 41.0 56-57 34.230176594306 38.0 33.5 40.0 24.0 41.0 58-59 33.86115879383226 37.5 33.0 40.0 23.0 41.0 60-61 33.43527115543368 37.0 32.5 40.0 22.0 41.0 62-63 33.127556925425516 36.0 32.0 39.0 22.0 40.5 64-65 32.72024591286023 36.0 31.5 39.0 20.5 40.0 66-67 32.2867386263228 35.5 31.0 38.5 19.5 40.0 68-69 31.930317637444464 35.0 31.0 38.0 18.5 40.0 70-71 31.546160515388223 35.0 31.0 37.5 17.5 40.0 72-73 31.080808177183368 34.5 30.0 37.0 14.0 39.0 74-75 30.602370354391624 34.0 30.0 36.5 10.0 39.0 76-77 29.10573838157147 32.5 28.0 35.0 8.0 37.0 78-79 29.866289030577324 34.0 29.0 35.0 7.0 37.0 80-81 29.613437262767516 34.0 29.0 35.0 7.0 37.0 82-83 29.31402715495248 34.0 29.0 35.0 2.0 36.5 84-85 28.949821829329505 34.0 29.0 35.0 2.0 36.0 86-87 28.589639966647447 34.0 29.0 35.0 2.0 36.0 88-89 28.122555701668873 33.0 27.5 35.0 2.0 35.0 90-91 27.65063199771012 33.0 26.5 35.0 2.0 35.0 92-93 27.05749333156338 33.0 25.5 34.5 2.0 35.0 94-95 26.52980158549081 32.0 25.0 34.0 2.0 35.0 96-97 25.83049726002962 32.0 24.0 34.0 2.0 35.0 98-99 24.973967584968122 32.0 19.0 34.0 2.0 35.0 100-101 21.783329115866938 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.43298669552955715 1101 2 0.37260042997488085 1101 3 0.3819490738885918 1101 4 0.3086726815402159 1101 5 0.3806767792175165 1101 6 0.334979927656299 1101 7 0.3112501770985645 1101 8 0.2571559193743269 1101 9 0.3576222712666848 1101 10-11 0.4662827887574039 1101 12-13 0.5170181513383838 1101 14-15 0.6509002593114559 1101 16-17 0.7180089448987701 1101 18-19 0.5595526682074095 1101 20-21 0.382705595440882 1101 22-23 0.4364699778427408 1101 24-25 0.45581703255768957 1101 26-27 0.42214568920908135 1101 28-29 0.5742805413022083 1101 30-31 0.6780193272361643 1101 32-33 0.7280874624948694 1101 34-35 0.7169546290679776 1101 36-37 0.7173942559663473 1101 38-39 0.5609624179352082 1101 40-41 0.6554189554298802 1101 42-43 0.8242839928469579 1101 44-45 0.7522938669715487 1101 46-47 0.7398747394647174 1101 48-49 0.6872957548953167 1101 50-51 0.772694946775168 1101 52-53 0.8367887558197893 1101 54-55 0.7327575533573878 1101 56-57 0.916153373009351 1101 58-59 0.5745238069121754 1101 60-61 0.7451347311720582 1101 62-63 0.6308761023618459 1101 64-65 0.6328637882176196 1101 66-67 0.6894305757909436 1101 68-69 0.7374013363419607 1101 70-71 0.5615199358020355 1101 72-73 0.808197016666611 1101 74-75 0.5952114359074301 1101 76-77 0.6372406077327888 1101 78-79 0.9107772403676613 1101 80-81 0.9296035017781747 1101 82-83 0.9010737591215054 1101 84-85 0.9295526811620256 1101 86-87 1.0028133662834513 1101 88-89 0.8905879760133182 1101 90-91 0.9692142491141347 1101 92-93 1.031573653684756 1101 94-95 1.2634938887580809 1101 96-97 1.656677052896395 1101 98-99 1.1926017447812072 1101 100-101 1.7355928647718386 1102 1 0.33045380898712295 1102 2 0.3514220591545083 1102 3 0.3127748334732985 1102 4 0.19135591804207053 1102 5 0.286416756556541 1102 6 0.110290712513617 1102 7 0.3262932088480426 1102 8 0.29759612091238097 1102 9 0.34031515073760943 1102 10-11 0.32484755334842674 1102 12-13 0.3569448647734461 1102 14-15 0.4518543105638386 1102 16-17 0.5143363799493557 1102 18-19 0.364017362886905 1102 20-21 0.3440694326997047 1102 22-23 0.2563222395207845 1102 24-25 0.4144376460112369 1102 26-27 0.32201751807851764 1102 28-29 0.42955464269802945 1102 30-31 0.4104219131590341 1102 32-33 0.352382320381615 1102 34-35 0.4567893325065029 1102 36-37 0.4286234934485762 1102 38-39 0.4294323198179981 1102 40-41 0.38666639401623115 1102 42-43 0.5076490676201502 1102 44-45 0.4185426716451772 1102 46-47 0.3903271554302563 1102 48-49 0.5027594611704771 1102 50-51 0.5338365939549945 1102 52-53 0.669828782177234 1102 54-55 0.6166863400308102 1102 56-57 0.4357245823180307 1102 58-59 0.5047408540743348 1102 60-61 0.645232567129078 1102 62-63 0.5457799137453705 1102 64-65 0.270978226153062 1102 66-67 0.10784703955346941 1102 68-69 0.39072655350966556 1102 70-71 0.4751972820569428 1102 72-73 0.5294097405642972 1102 74-75 0.3090693869372174 1102 76-77 0.35233707760253097 1102 78-79 0.5421948371790499 1102 80-81 0.5876728588332121 1102 82-83 0.5297953424970281 1102 84-85 0.5776727039837191 1102 86-87 0.5678853269401394 1102 88-89 0.7163666457657349 1102 90-91 0.7197275363219333 1102 92-93 0.6557188446557198 1102 94-95 0.5061592054090767 1102 96-97 0.47896818216842973 1102 98-99 0.5461993758251396 1102 100-101 1.2765483624695797 1103 1 0.09604985481627892 1103 2 0.1464797061200187 1103 3 0.15169080133424728 1103 4 0.1556062671444991 1103 5 0.16476387372757273 1103 6 0.12753793471581076 1103 7 0.06720092323057969 1103 8 7.779401866798707E-4 1103 9 0.11052643882342039 1103 10-11 0.1874216700171374 1103 12-13 0.20419963383708506 1103 14-15 0.21573669008444085 1103 16-17 0.2188875700221118 1103 18-19 0.07969746969662594 1103 20-21 0.044956249005736026 1103 22-23 0.10112127429162143 1103 24-25 0.1605557591997382 1103 26-27 0.06634956075433962 1103 28-29 0.003993074489066828 1103 30-31 -0.030322906785833936 1103 32-33 0.0046369452377064135 1103 34-35 -0.0076313617566796665 1103 36-37 -0.22573415808351172 1103 38-39 -0.06332425544511722 1103 40-41 0.02761905308315704 1103 42-43 0.066874805301822 1103 44-45 0.012031220364491446 1103 46-47 0.15324815132755987 1103 48-49 0.05562023840106889 1103 50-51 0.18519863420332427 1103 52-53 0.07497627044234889 1103 54-55 0.2109953695861435 1103 56-57 0.017687343123377275 1103 58-59 0.16994681849833881 1103 60-61 0.024695189735538747 1103 62-63 0.03763248797019969 1103 64-65 0.04703953358671953 1103 66-67 -0.05431224008177793 1103 68-69 0.021928379177161617 1103 70-71 0.03591646911095481 1103 72-73 -0.0027485416354409153 1103 74-75 0.02494177047159951 1103 76-77 -0.24326891854712684 1103 78-79 -0.29663550745294387 1103 80-81 -0.11388271995578236 1103 82-83 -0.19714406870356882 1103 84-85 0.03383060643692559 1103 86-87 -0.03893340658114397 1103 88-89 -0.1634531909432546 1103 90-91 -0.10068540197886833 1103 92-93 -0.06833911956638516 1103 94-95 -0.2103762323930063 1103 96-97 0.10771817916410598 1103 98-99 0.06469249111666642 1103 100-101 0.3847010157682078 1104 1 0.1319714371838785 1104 2 0.049799112644024035 1104 3 0.1300519111599172 1104 4 0.11676269914883619 1104 5 0.09530666671392396 1104 6 0.07552174259635791 1104 7 0.07817544730610848 1104 8 0.14786829476941676 1104 9 0.1942360384056201 1104 10-11 0.06943410498312375 1104 12-13 0.15771394958341745 1104 14-15 0.14968736449899467 1104 16-17 0.37723891785960006 1104 18-19 0.08406474742408676 1104 20-21 0.1512812130318153 1104 22-23 0.09718452668639799 1104 24-25 0.1087568980249074 1104 26-27 0.1742933510941569 1104 28-29 0.17172931790853596 1104 30-31 0.22260541421831448 1104 32-33 0.036115776267166666 1104 34-35 -0.041717062317054854 1104 36-37 -0.0902223919128815 1104 38-39 -0.12369598225929934 1104 40-41 -0.08325608822327979 1104 42-43 -0.23042428615015353 1104 44-45 -0.32083715145487446 1104 46-47 -0.31615418582022414 1104 48-49 -0.4475372579464505 1104 50-51 -0.17150452422955453 1104 52-53 -0.10991754865457182 1104 54-55 -0.27477399848488915 1104 56-57 -0.31466318056431675 1104 58-59 -0.2142133089584135 1104 60-61 0.04620985144848788 1104 62-63 0.0651062595132359 1104 64-65 0.04323390072121214 1104 66-67 0.18284760531300748 1104 68-69 0.05303948294291416 1104 70-71 -0.026830748281692962 1104 72-73 0.15272268914633003 1104 74-75 0.026172210686585373 1104 76-77 -0.11253099597754712 1104 78-79 -0.08951746452222764 1104 80-81 0.10614186130340286 1104 82-83 0.22211789854639008 1104 84-85 0.41813286667875005 1104 86-87 0.23915379463701214 1104 88-89 0.19242196138122125 1104 90-91 0.29206990265906896 1104 92-93 0.35617267084394655 1104 94-95 0.21755308023521636 1104 96-97 0.36573793892664597 1104 98-99 0.3573436201357758 1104 100-101 0.8766782260431967 1105 1 -0.02906710493671838 1105 2 -0.021965355448450907 1105 3 0.07989892438982338 1105 4 0.04602434607119221 1105 5 0.14519653381523057 1105 6 0.051473948898724586 1105 7 0.011634602500706137 1105 8 0.13269730058544837 1105 9 0.08256422565584387 1105 10-11 0.10717523554992425 1105 12-13 0.12073426355909334 1105 14-15 0.1274687435872366 1105 16-17 0.04180889812820965 1105 18-19 0.029188788445942748 1105 20-21 -0.04912034170043711 1105 22-23 0.13281656442675427 1105 24-25 0.09411860485997181 1105 26-27 0.14378387594293685 1105 28-29 0.13310138617904244 1105 30-31 0.19412981792142148 1105 32-33 0.12307114052249091 1105 34-35 0.211842677995719 1105 36-37 0.15566784944901713 1105 38-39 0.2151871600408839 1105 40-41 0.09520573176875047 1105 42-43 0.023636363708902763 1105 44-45 0.10444280735578815 1105 46-47 0.21705678911310144 1105 48-49 0.12629417280939492 1105 50-51 0.11827035595794655 1105 52-53 0.23942007730298798 1105 54-55 0.07675206974737137 1105 56-57 -0.11434175068208674 1105 58-59 -0.09723154485734398 1105 60-61 0.1267309317795977 1105 62-63 0.19875993716630802 1105 64-65 0.2144638068303948 1105 66-67 0.0808298031221355 1105 68-69 0.05572573315679463 1105 70-71 0.24582041758656814 1105 72-73 0.4288623875977535 1105 74-75 0.17697312317939407 1105 76-77 0.047237696503795945 1105 78-79 -0.11823602004177403 1105 80-81 -0.23426856402514673 1105 82-83 0.12827489879606802 1105 84-85 0.17515064043822193 1105 86-87 0.061491930808934114 1105 88-89 0.10991895411467212 1105 90-91 0.18706396049680407 1105 92-93 0.09552524137836826 1105 94-95 0.24422250733219997 1105 96-97 0.04211392814545789 1105 98-99 -0.3174712268438533 1105 100-101 0.22551959486511564 1106 1 0.1119076884040382 1106 2 0.18925535128096982 1106 3 0.1061484318088759 1106 4 0.14045954239684733 1106 5 0.14623938198941744 1106 6 0.07219706022226546 1106 7 0.057550388515053896 1106 8 0.07662972722022943 1106 9 0.11341664915408955 1106 10-11 -0.02429155937507943 1106 12-13 0.1254278295154947 1106 14-15 0.050095702111548235 1106 16-17 0.2039830268030869 1106 18-19 0.36798583710569943 1106 20-21 0.12328973137385901 1106 22-23 0.11405004205071378 1106 24-25 0.05253028915466729 1106 26-27 0.1336600577001903 1106 28-29 -0.1541373586273167 1106 30-31 0.09109387112466294 1106 32-33 0.19505363585638946 1106 34-35 0.10735083923163558 1106 36-37 -0.07659211941537336 1106 38-39 0.1829967143881106 1106 40-41 0.3723264019884951 1106 42-43 0.2995795844216218 1106 44-45 0.2650571588046091 1106 46-47 0.0887315243680149 1106 48-49 -0.04308949293179154 1106 50-51 0.02747139341207827 1106 52-53 -0.12499967108491461 1106 54-55 0.015292202171110603 1106 56-57 -0.1574508866414277 1106 58-59 -0.1727943357548014 1106 60-61 -0.01366450146438325 1106 62-63 0.0804004152582607 1106 64-65 0.1480446667705877 1106 66-67 0.26992544698348553 1106 68-69 0.21036743140108172 1106 70-71 0.27722348290092924 1106 72-73 0.4435329339792702 1106 74-75 0.3799679154313509 1106 76-77 0.15887164680568944 1106 78-79 0.0031349257486006366 1106 80-81 0.4286345095301094 1106 82-83 0.23522926769561892 1106 84-85 0.5840824752267011 1106 86-87 0.8331516326682333 1106 88-89 0.573257696473739 1106 90-91 0.6342855043016691 1106 92-93 0.8006207134294883 1106 94-95 0.7981427024766639 1106 96-97 0.8540676252869623 1106 98-99 0.9099887935636062 1106 100-101 1.350433127867916 1107 1 0.0075868638797231824 1107 2 0.008467075150036862 1107 3 0.027128707100139593 1107 4 0.09566942199440831 1107 5 -0.008924015968389654 1107 6 -0.11385460561972849 1107 7 -0.004957108523612419 1107 8 0.0613816876958424 1107 9 -0.08394992230018516 1107 10-11 0.021297698493064843 1107 12-13 -0.06436415577531562 1107 14-15 0.015127639496277823 1107 16-17 0.05511784417522847 1107 18-19 0.0019722093636573845 1107 20-21 -0.06595543865898179 1107 22-23 -0.07056289521406 1107 24-25 -0.08580038272993562 1107 26-27 0.03989342364564408 1107 28-29 -0.13634969725355717 1107 30-31 -0.1985600749416463 1107 32-33 -0.12964545175400133 1107 34-35 -0.1658147759753561 1107 36-37 -0.07236168106351926 1107 38-39 -0.2120133503863073 1107 40-41 -0.31368028162147965 1107 42-43 -0.39729124535061544 1107 44-45 -0.6040600086642414 1107 46-47 -0.5915120450470681 1107 48-49 -0.642202686970144 1107 50-51 -0.40375147667923983 1107 52-53 -0.4021923674698442 1107 54-55 -0.5371254522303985 1107 56-57 -0.7315694223198363 1107 58-59 -0.7062986015698058 1107 60-61 -0.6080234015738384 1107 62-63 -0.42225494212558345 1107 64-65 -0.5403678634049882 1107 66-67 -0.5857771754806365 1107 68-69 -0.6530009131028365 1107 70-71 -0.4473587446191978 1107 72-73 -0.33331115010751233 1107 74-75 -0.518288367087056 1107 76-77 -0.651690368698187 1107 78-79 -0.6681895104740896 1107 80-81 -0.5065566368420704 1107 82-83 -0.5705742367951068 1107 84-85 -0.4996742065090167 1107 86-87 -0.3230147284941083 1107 88-89 -0.582033853988829 1107 90-91 -0.5185971539233307 1107 92-93 -0.30093136100111195 1107 94-95 -0.2116296874293262 1107 96-97 -0.14803787116693456 1107 98-99 -0.42288042621958155 1107 100-101 -0.07173238238053514 1108 1 0.05162926154054048 1108 2 0.11728410886908591 1108 3 0.028302963663534797 1108 4 -0.010550249524818867 1108 5 0.13061032520369054 1108 6 0.10954576143265626 1108 7 0.12378340410547395 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-1.128140369562054 2316 62-63 -0.8208750153728701 2316 64-65 -0.8446366257582056 2316 66-67 -0.7929478533087959 2316 68-69 -1.2471711646239783 2316 70-71 -1.4095845516958967 2316 72-73 -0.7791725809488881 2316 74-75 -0.9716525592352525 2316 76-77 -0.7157841756198629 2316 78-79 -0.9438896470861131 2316 80-81 -0.7191173129262474 2316 82-83 -0.9004662309430707 2316 84-85 -0.8955287340833245 2316 86-87 -0.9005232788564683 2316 88-89 -1.0489508549189566 2316 90-91 -1.1115593337086267 2316 92-93 -1.0871408133903664 2316 94-95 -1.3347839718148613 2316 96-97 -1.2420161634197733 2316 98-99 -2.1305615824804782 2316 100-101 -2.644126459619617 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 6.0 4 19.0 5 35.0 6 91.0 7 222.0 8 494.0 9 955.0 10 1642.0 11 2715.0 12 3691.0 13 5080.0 14 6583.0 15 8912.0 16 11265.0 17 14503.0 18 13081.0 19 8391.0 20 7462.0 21 7398.0 22 8049.0 23 9010.0 24 10224.0 25 12278.0 26 15334.0 27 18949.0 28 23197.0 29 29192.0 30 35847.0 31 44329.0 32 56030.0 33 70803.0 34 92896.0 35 126114.0 36 178486.0 37 232315.0 38 141570.0 39 8137.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.97151290790241 19.016428541198323 15.168330930917474 21.84372761998179 2 15.680570099956684 21.350867381061832 38.8494276927979 24.119134826183572 3 20.61187591220889 24.58282130704236 30.139174346715304 24.666128434033453 4 12.98360166099037 17.124877105794802 37.56144710260059 32.33007413061424 5 13.428161088815562 37.54668029566746 33.90725586651323 15.117902749003754 6 30.97639186761857 36.47392153853174 17.258204354914316 15.291482238935373 7 28.091478920273293 33.18172578724887 20.72031560476394 18.0064796877139 8 26.496778823617255 33.09054554656291 19.70663027200584 20.706045357814 9 25.98670046170886 17.49673319201364 20.995847524070673 35.520718822206824 10-11 25.160146187064687 26.23672016626497 27.024238678176893 21.57889496849345 12-13 27.003981184681418 23.85703245140503 27.53285607791291 21.606130286000642 14-15 23.1171044329462 25.32866176140552 27.33703391964437 24.21719988600391 16-17 21.572008744674584 28.574427219666394 27.726757957529426 22.1268060781296 18-19 21.22409493810828 27.330921466650572 30.37288520900148 21.072098386239666 20-21 22.348119355681757 25.589954409879656 30.320665723613526 21.74126051082506 22-23 21.735654851933496 25.714695806780536 30.50672735403463 22.042921987251336 24-25 22.427393896150765 25.832921957512827 30.509580562596188 21.230103583740213 26-27 22.04605379967908 25.54725600396918 31.162396310917078 21.244293885434658 28-29 21.162317000825546 26.81588696261818 30.790407088897464 21.231388947658814 30-31 21.559980884173356 25.879917098376815 30.562962754006524 21.997139263443305 32-33 21.67109529113798 26.051969441454 30.56655278775169 21.71038247965633 34-35 22.662644374806582 25.664180113716036 30.181791330334867 21.49138418114252 36-37 22.44058720845336 25.284992234272742 30.863829300022566 21.41059125725133 38-39 21.86083461068579 26.049485913170457 30.751456596635258 21.3382228795085 40-41 21.86791802403459 26.171602279755863 30.466243177553885 21.49423651865566 42-43 21.65118242367295 26.46753909434291 29.60450360192739 22.276774880056752 44-45 22.099292121743115 25.972627379921214 29.432162496722786 22.49591800161289 46-47 22.714882421827845 26.572496825490987 29.014867197459232 21.697753555221936 48-49 22.715851593978766 26.60176727565676 28.781387893402606 21.90099323696187 50-51 21.962665865762553 26.82334629962802 29.30313460208603 21.9108532325234 52-53 22.60706337205805 27.458542565040727 27.98853399872646 21.94586006417476 54-55 21.915296452460247 26.741287959870107 28.635402717695218 22.70801286997443 56-57 22.646974258889777 26.799456824113822 28.307877668426222 22.24569124857018 58-59 23.31335667240254 26.44437980579914 28.076427221979944 22.16583629981838 60-61 22.472275147379044 26.65199791544957 29.379381539115197 21.496345398056192 62-63 22.216791729350135 26.86127377235134 28.98059045961055 21.941344038687973 64-65 21.891286245421025 27.510236698101114 28.512103347397165 22.086373709080696 66-67 22.123591301267176 27.06701104059724 28.04815316619638 22.761244491939213 68-69 21.951689995594453 26.939995801871568 28.431119581116388 22.67719462141759 70-71 22.627569476248315 26.974232226890475 28.09585797441973 22.302340322441474 72-73 22.500114079009048 26.451396119654362 28.749818510667424 22.298671290669166 74-75 21.970806038579305 27.820301198846582 27.895055395680767 22.31383736689335 76-77 22.23516527279968 26.972072879330945 27.936620536306915 22.856141311562457 78-79 22.24533949548107 27.082639296552667 28.141917470643023 22.53010373732324 80-81 22.676880221685536 27.227590704907062 27.762138623161558 22.333390450245844 82-83 22.41577819055826 27.006575767369938 28.341354062898176 22.236291979173625 84-85 22.0595191857628 27.35784543170041 28.005254963666726 22.577380418870067 86-87 22.24358357368311 27.31005453091136 27.72832952203308 22.718032373372456 88-89 22.0176956628196 27.290911882737184 27.334736038312933 23.356656416130278 90-91 22.101436644222215 26.697984315444174 28.02614147974023 23.174437560593383 92-93 22.57252144065251 26.87513382253596 27.941232611369397 22.611112125442133 94-95 22.05903650204472 26.784446431646586 28.141617891458647 23.014899174850044 96-97 21.474398350444265 26.832341651946397 28.720921931653677 22.972338065955668 98-99 21.81240645599914 27.12869413197393 27.56562710902032 23.493272303006606 100-101 21.182970838532288 26.82797747576104 28.142887622045727 23.846164063660943 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 266.0 1 204.5 2 290.0 3 416.5 4 312.0 5 260.0 6 416.0 7 786.0 8 1215.5 9 1529.0 10 1539.5 11 1409.5 12 1461.5 13 1614.5 14 1745.5 15 1913.5 16 2113.5 17 2201.5 18 2354.0 19 2535.0 20 2743.5 21 3087.5 22 3453.0 23 3975.5 24 4664.0 25 5553.5 26 6850.0 27 8576.0 28 10054.5 29 11722.0 30 14201.0 31 16852.0 32 19418.0 33 21512.5 34 23673.0 35 25819.0 36 28193.0 37 30312.5 38 31151.5 39 31738.0 40 33088.0 41 35683.0 42 37890.5 43 39390.0 44 40951.0 45 42640.0 46 43515.5 47 49491.0 48 65178.5 49 63455.0 50 47524.0 51 40821.0 52 38276.0 53 37388.0 54 35818.0 55 33195.5 56 30159.0 57 27103.0 58 25086.5 59 22585.0 60 18954.0 61 15499.0 62 12470.5 63 9837.5 64 7291.5 65 5495.5 66 4310.0 67 3492.5 68 2653.0 69 1853.5 70 1372.5 71 1024.5 72 880.5 73 759.5 74 607.0 75 506.5 76 372.5 77 235.0 78 155.5 79 105.0 80 66.0 81 47.0 82 35.5 83 18.5 84 9.0 85 5.5 86 3.5 87 0.5 88 2.0 89 2.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02223503594525867 2 0.015182879022322151 3 0.010453785556352957 4 0.0 5 0.0026549296651055126 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 8.711487963627463E-4 14-15 4.1483276017273635E-5 16-17 0.0 18-19 0.0012030150045009354 20-21 0.0 22-23 1.6593310406909454E-4 24-25 0.0 26-27 0.0015763644886563981 28-29 0.003318662081381891 30-31 0.0017422975927254927 32-33 0.006429907782677414 34-35 0.00182526414476004 36-37 7.466989683109254E-4 38-39 0.0010370819004318409 40-41 4.1483276017273635E-5 42-43 0.0039409112216409956 44-45 0.0010785651764491145 46-47 4.1483276017273635E-5 48-49 2.9038293212091545E-4 50-51 4.1483276017273635E-5 52-53 2.0741638008636818E-4 54-55 0.0015763644886563981 56-57 0.01414579712189031 58-59 0.026175947166899662 60-61 0.02065867145660227 62-63 0.01460211315808032 64-65 0.01696665989106492 66-67 0.002157130352898229 68-69 4.977993122072836E-4 70-71 8.296655203454727E-5 72-73 0.0 74-75 0.0017837808687427663 76-77 0.0020741638008636818 78-79 0.008089238823368358 80-81 0.0144776633300285 82-83 0.016468860578857634 84-85 0.028955326660057 86-87 0.00970708658804203 88-89 0.040653610496928165 90-91 0.045880503275104643 92-93 0.029121259764126092 94-95 0.03090504063286886 96-97 0.05119036260531567 98-99 0.0545919912387321 100-101 0.06106338229742679 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1205305.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.1038524642879 #Duplication Level Percentage of deduplicated Percentage of total 1 83.17096751578016 49.157245933647516 2 10.026903654771994 11.852572685705459 3 2.7438962814498433 4.865245229883591 4 1.1936729543316509 2.822026807337142 5 0.6565613285485841 1.9402651948146186 6 0.39868937584213493 1.413844682931154 7 0.28573282921954146 1.1821537687676744 8 0.2119540182413094 1.002183921867787 9 0.16428972858754842 0.8739140291852727 >10 1.0166169303587358 11.399954211564948 >50 0.08281584795002829 3.3330375669205625 >100 0.04298316616146298 4.6772863141514 >500 0.0023879505391733876 1.0068054852996948 >1k 0.002107015181623577 2.7843854511405266 >5k 4.2140303632471547E-4 1.6890787167825754 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8900 0.7384023131074707 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5911 0.4904152890762089 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5534 0.45913689895918464 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4723 0.3918510252591667 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3269 0.271217658600935 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3253 0.26989019376838225 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3216 0.266820431343104 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2385 0.19787522660239523 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2262 0.18767034070214592 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2257 0.1872555079419732 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1702 0.14120907156279947 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1692 0.14037940604245397 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1664 0.13805634258548666 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1525 0.1265239918526846 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1470 0.12196083149078449 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1441 0.11955480148178263 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1423 0.11806140354516077 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1256 0.10420598935539137 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 8.296655203454727E-5 0.0 0.0 0.0 0.0 3 8.296655203454727E-5 0.0 0.0 0.0 0.0 4 8.296655203454727E-5 0.0 0.0 0.0 0.0 5 8.296655203454727E-5 0.0 0.0 0.0 0.0 6 8.296655203454727E-5 0.0 0.0 0.0 0.0 7 8.296655203454727E-5 0.0 0.0 0.0 0.0 8 1.6593310406909454E-4 0.0 0.0 0.0 0.0 9 1.6593310406909454E-4 0.0 0.0 0.0 0.0 10-11 1.6593310406909454E-4 0.0 0.0 0.0 0.0 12-13 1.6593310406909454E-4 0.0 0.0 0.0 0.0 14-15 1.6593310406909454E-4 0.0 0.0 0.0 0.0 16-17 1.6593310406909454E-4 0.0 0.0 0.0 0.0 18-19 3.733494841554627E-4 0.0 0.0 0.0 0.0 20-21 4.977993122072836E-4 0.0 0.0 1.244498280518209E-4 0.0 22-23 4.977993122072836E-4 0.0 0.0 2.488996561036418E-4 0.0 24-25 4.977993122072836E-4 0.0 0.0 4.977993122072836E-4 0.0 26-27 4.977993122072836E-4 0.0 0.0 6.222491402591045E-4 0.0 28-29 7.052156922936518E-4 0.0 0.0 9.1263207238002E-4 0.0 30-31 7.466989683109254E-4 0.0 0.0 0.0022400969049327763 0.0 32-33 7.466989683109254E-4 0.0 0.0 0.0041483276017273635 0.0 34-35 7.881822443281991E-4 0.0 0.0 0.006139524850556499 0.0 36-37 8.296655203454727E-4 0.0 0.0 0.008255171927437454 0.0 38-39 9.541153483972936E-4 0.0 0.0 0.01198866676899208 0.0 40-41 9.955986244145672E-4 0.0 0.0 0.015182879022322151 0.0 42-43 9.955986244145672E-4 0.0 0.0 0.021322403872878648 0.0 44-45 0.0010785651764491145 0.0 0.0 0.028913843384039725 0.0 46-47 0.0012030150045009354 0.0 0.0 0.03857944669606449 0.0 48-49 0.001244498280518209 0.0 0.0 0.049779931220728366 0.0 50-51 0.001244498280518209 0.0 0.0 0.06218343074989319 0.0 52-53 0.001244498280518209 0.0 0.0 0.08296655203454727 0.0 54-55 0.001244498280518209 0.0 0.0 0.09951837916543946 0.0 56-57 0.001244498280518209 0.0 0.0 0.13382504843172474 0.0 58-59 0.001244498280518209 0.0 0.0 0.18003741791496758 0.0 60-61 0.0012859815565354827 0.0 0.0 0.2243415567014158 0.0 62-63 0.0013274648325527563 0.0 0.0 0.29108814781320913 4.1483276017273635E-5 64-65 0.0013274648325527563 0.0 0.0 0.3370931009163656 8.296655203454727E-5 66-67 0.0013274648325527563 0.0 0.0 0.42632362762952114 8.296655203454727E-5 68-69 0.0013274648325527563 0.0 0.0 0.5445094810027338 8.296655203454727E-5 70-71 0.0013274648325527563 0.0 0.0 0.6194282774899299 1.244498280518209E-4 72-73 0.0013274648325527563 0.0 0.0 0.7432973396775091 2.488996561036418E-4 74-75 0.0013274648325527563 0.0 0.0 0.8768734884531302 2.488996561036418E-4 76-77 0.0013274648325527563 0.0 0.0 0.9590518582433492 2.488996561036418E-4 78-79 0.0013274648325527563 0.0 0.0 1.0507298982415239 2.488996561036418E-4 80-81 0.0015763644886563981 0.0 0.0 1.2198157312879312 2.488996561036418E-4 82-83 0.0017422975927254927 0.0 0.0 1.3600706875023334 2.488996561036418E-4 84-85 0.00182526414476004 0.0 0.0 1.5671552013805634 2.488996561036418E-4 86-87 0.0019082306967945872 0.0 0.0 1.8887750403424861 2.488996561036418E-4 88-89 0.0019082306967945872 0.0 0.0 2.310701440714176 2.488996561036418E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1345 0.0 36.384678 2 GTACCTG 1450 0.0 34.741585 1 GAGTACT 6400 0.0 23.080215 12-13 GTACATG 5165 0.0 22.818817 1 TACATGG 5240 0.0 22.214596 2 ACCTGGG 2180 0.0 22.008804 3 AGTACTT 6655 0.0 21.517841 12-13 ACATGGG 5200 0.0 21.194159 3 GTACTTT 7035 0.0 20.828142 14-15 GTACACG 195 1.0282451E-5 19.496971 1 TGCACCG 200 1.2856899E-5 18.998507 5 TACTTTT 7725 0.0 18.475893 14-15 AGAGTAC 10885 0.0 18.370169 10-11 ACTTTTT 8155 0.0 18.229713 16-17 CATGGGG 2425 0.0 18.215063 4 ATACGTC 290 5.2183168E-8 18.015825 5 CCACGTC 345 1.0841177E-9 17.897144 9 GTATTAG 350 1.3023964E-9 17.651722 1 GTATGCT 340 1.751323E-8 16.763388 4 CATGGGA 2910 0.0 16.484959 4 >>END_MODULE