FastQCFastQC Report
Thu 26 May 2016
SRR936491_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936491_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1011434
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111621.1035816474431352No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85130.8416762734889276No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53850.5324123966566281No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT40640.4018057530199697No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT25410.25122746516332256No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22540.22285191124680406No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22320.22067678167829044No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21240.2099988728874054No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA17590.17391149595524769No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT16570.16382680431941185No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC15610.15433532983862516No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA14520.14355855152189861No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG13180.13031003505913386No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13080.12932133980071858No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT11620.1148863890278555No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10890.10766891364142395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG10500.042.0753062
TATCACG905.6929457E-736.9478532
ATCACGC1054.6487912E-836.197393
GTACCTG13750.032.4741131
GTACATG118150.026.9774531
ACCTGGG16300.026.2320063
GTATCAA169900.026.0855771
TACATGG120300.026.0227642
TCACGCA1309.9969875E-625.5843434
ACATGGG117550.025.2196223
GTATCAC2101.2641976E-924.8820131
ATGGGAT31750.024.6934225
GAGTACT85800.024.3094612-13
CATGGGG23350.024.2147314
GTAAGGT3600.023.756894
ATGGGAG28300.023.5062125
CATGGGT7800.023.147744
CATGGGA90350.023.0337984
AGTACTT88450.022.8291212-13
GGTATCA124400.022.7582071
Produced by FastQC (version 0.11.5)